http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
배성준,김우미,김기찬,장명웅,강구일,Bae, Sung-Jun,Kim, Woo-Mi,Kim, Ki-Chan,Chang, Myung-Woong,Kang, Koo-Il The Korean Society of Pharmacology 1991 대한약리학잡지 Vol.27 No.2
염증성 질환의 원인 인자중 하나로 알려진 사람 호중구 Cathepsin G를 두단계의 크로마토그라피를 거쳐 분리하였다. 이 순수 분리된 효소를 이용하여 토끼에서 항체를 In Vivo 합성하고 그 혈액으로부터 순수 항체를 분리하였다. NSAIDs 약제중 phenylbutazone, sulindac, oxyphenbutazone, salicilic acid등은 이 효소를 강력하게 억제하였으며 $IC_{50}$은 $0.3{\sim}0.8\;mM$ 이었다. 고려인삼의 지용성분획도 tetracycline, novobiosin, rifamycin이 Cathepsin G의 효소 활성도에 대해서 강력한 억제 작용을 나타내었으나 다른 항생제는 그 작용이 무시할 수 있을 정도였다. 그러나 tetracycline계열의 항생제의 경우 실제 치료 효과를 나타내는 혈중농도에서 강한 억제 작용을 보였다. 특히 항균 작용과 관계하는 tetracycline의 4번 위치의 N-dimethy radical을 제거한 tetracycline은 감염군의 약제 저항성을 피할 수 있을 것으로 생각되므로 만성 염증성질환의 장기 치료에 이용될 수 있는 새로운 약제로써 제시한다. Human neutrophil cathepsin-G, which has been known as one of the active enzymes causing inflammatory diseases, was purified by two steps procedure involving one size exclusion (Ultorogel AcA54) and one ion exchange (CM-Sephadex) chromatography. Purified HNCGs were cross-reacted with Anti-HNCathepsin-G antibodies which were radised in rabbits and purified by cathepsin-G labeled Sepharose 4B affinity chromatography. HNCGs were effectively inhibited by NSAIDs including phenylbutazone, sulindac, oxyphenbutazone, salicylic acid and salicyluric acid. $IC_{50}_s$ of these drugs for inhibition of Cathepsin G were 0.3-0.8 mM. Other NSAIDs including aspirin showed little or no inhibition effect on the activity of Cathepsin G. These results strongly indicated that NSAIDs which showed inhibition effect on the activity of HNCGs possibly be at least a part of mechanism of action which might be related to direct inhibition of cathepsin G at the tissue destruction sites beside of their known mechanism of action as an anticyclo-oxygenase in treatment of inflammatory diseases. Lipid soluble component of Korean Red Ginseng which was known as an anti-inflammatory agent inhibited HNCGs strongly, but no other fractions did inhibited HNCGs. Antibiotics including novobiosin and rifamycin showed some inhibition effect on HNCGs, i. e.., $IC_{50}$ of these drugs were 2.6 mM and 1.5 mM respectively, and other antibiotics including penicillin G showed no or negligible inhibition effect on the activity of HNCGs. However. tetracyclines inhibited HNCGs very effectively at the concentration of therapeutic range. The inhibition effect of the activity of HNCGs by tetracycline are not related to the N-dimethyl radical on the 4 position of the tetracycline molecule. Furthermore, N-dedimethylated tetracyclines may have beneficial effect for long term treatment of chronic inflammatory diseases without developing any drug resistance to microorganisms.
사람중성구 Cathepsin G의 분리와 Anti-Human Cathepsin G Antibody의 in vivo 합성 및 분리
배성준,김사열,강구일 고신대학교 의학부 1989 高神大學校 醫學部 論文集 Vol.5 No.1
Human neurtrophil cathepsin G was purified by two step procedure involving ultrogel AcA54 gel filtration and CM-Sephadex ion exchange chromatography. Highly purified human neutrophil cathepsin G was injected to rabbit for in vivo synthesis of anti-human cathepsin G antibodies. For purificaion of Ab, precipitation by ammonium sulfate, DEAD-cellulose ion exchange chromatography and CNBr-activated sepharose 4B affinity chromatography was applied Purified Antibodies detected by immumoprecipitation showed specificity against human neutrophil cathepsin G but not human neutrophil elastsae.
스케일러블 비디오 코딩을 이용한 환경적응형 미디어 시스템
배성준,정순흥,강정원,유정주,Bae, Seong-Jun,Jung, Soon-heung,Kang, Jung-won,Yoo, Jeong-Ju 대한임베디드공학회 2008 대한임베디드공학회논문지 Vol.3 No.4
This paper describes the SVC-based media adaptation system which can adapt video contents optimally to various consumption environments in an IP-based transmission scenario. As key technologies, we will present scalable video coding, SVC-based adaptive media transmission, and SVC-based adaptation signaling technology.
비스테로이드성 항염증제에 의한 사람 백혈구 Cathepsin G의 효소 활성도 억제
배성준,김우미,강구일 고신대학교(의대) 고신대학교 의과대학 학술지 1990 고신대학교 의과대학 학술지 Vol.6 No.2
Human leukocyte cathepsin-Gs are active participant in the active phase of inflammations like rheumatoid arthritis, emphysema and glomerular injury. Inhibition effects of human leukocyte cathepsin-Gs by non-steroidal anti-inflammatory drugs were various. Among them, especially, sulindac, salicylate, phenylbutazone, oxyphenbutazone, and salicyluric acid inhibited human leukocyte cathepsin-Gs effectively IC50s of each drug were 8.3mM, 14.3mM, 6.5mM, 11mM, and 15mM respectively. The drugs which have same chemical structure and same degree of inhibition effect on cyclooxygenase showed different degree or no effect on inhibition of cathepsin G. These different inhibition effects may be, beside of inhibition of cyclooxygenase in the prostaglandin synthesis pathway, another possible mechanism of antiinflammatory effect of NSAIDs and the effect could be a direct protection of tissue destruction in inflammatory disease.