여러 종류의 동해방지제를 이용한 소 체외수정란의 동결 및 일단계 융해후의 체외배양 생존율 및 수태율

T. Suzuki,M. Takagi,M. Yamamoto,A. Boediono,S. Saha,H. Sakakibara,M. Oe 한국수정란이식학회 1997 한국수정란이식학회 학술대회 Vol.1997 No.2

치밀 난구세포로 둘러싸인 소 난자를 . 5% 배양기에시 5% superovulated cow serum(SCS)이 첨가된 m-TCM 199 medium 으로 시간 배양하였으며, 수정능이 획득된 정자와 체외수정하였다. 7일8일경의 수정란을 1.3M methyl cellosolve(MC), 1.1M diethylene glycol(DEG), 1.8M ethylene glycol(EG), 1.6M propylene glycol(PG) 및 1.1M 1,3-butylene glycol(BG) 용액에서 10분간 평형시킨 후 0.25 ml 스트로내에 장전하였다. 스트로를 의 alcohol bath freezer에 넣고 까지 /분 속도로 냉각, 식빙 후 10분간 정체시켰으며, /분 또는 /분으로 까지 냉각 후 스트로를 액체질소에 침지하여 보관하였다. 수정란이 들어있는 스트로를 온수에서 융해하였으며, 수정란을 TCM 199 medium 으로 옮긴 후 5% SCS가 첨가된 TCM 199 medium 에서 48시간 배양하였다. 수정란이 양호한 형태를 유지하며 나중의 발육단계로 진행된 것을 생존한 것으로 간주하였다. 각 종류의 동해방지제에서 동결된 수정란의 일부는 융해 후 동해방지제를 제거하지 않고 직접 비외과적으로 이식하였다. 동결-융해 후 동해방지제의 종류에 따른 탈출배반포 발달율은 EG 50.0%, MC 53.6%, DEG 56.9%, PG 58.0% 그리고 BG 11.5%였다. /분 또는 /분 으로 냉각한 수정란의 생존율은 두 그룹간에 유의적인 차이가 없었으나 (P<0.05), 탈출배반포 발달율은 -0.5분 /분(22.6%, 12/53)보다 /분(64.6%, 31/48) 냉각시에 유의적으로 높았다(P<0.01). 동해방지제의 종류에 따른 수정란의 수태율은 MC 48%(10/21). DEG 30%(3/10), EG 74%(20/27) 및 PG 40%(4/10) 였다. 이러한 결과로 보아 MC, DEG, EG 그리고 PG는 소의 체외수정란의 동결을 위한 동해방지제로서 이용될 수 있음을 보여주었다.

메니에르병의 진단기준

Jose A. Lopez-Escamez,John Careyb,Won-Ho Chung,Joel A. Goebeld,Måns Magnusson,Marco Mandalà,David E. Newman-Tokerg,Michael Strupp,Mamoru Suzuki,Franco Trabalzini,Alexandre Bisdorff 대한평형의학회 2015 Research in Vestibular Science Vol.14 No.3

This paper presents diagnostic criteria for Ménierè’s disease jointly formulated by the Classification Committee of the Bárány Society, The Japan Society for Equilibrium Research, the European Academy of Otology and Neurotology, the Equilibrium Committee of the American Academy of Otolaryngology-Head and Neck Surgery, and the Korean Balance Society. The classification includes two categories: definite Ménière's disease and probable Menière's disease. The diagnosis of definite Ménière's disease is based on clinical criteria and requires the observation of an episodic vertigo syndrome associated with low- to me-dium-frequency sensorineural hearing loss and fluctuating aural symptoms (hearing, tinnitus and/or fullness) in the affected ear. Duration of vertigo episodes is limited to a period between 20 minutes and 12 hours. Probable Ménierè's disease is a broader concept defined by episodic vestibular symptoms (vertigo or dizziness) associated with fluctuating aural symptoms occurring in a period from 20 minutes to 24 hours. Res Vestib Sci 2015;14(3):67-74

MOA-2011-BLG-293Lb: A TEST OF PURE SURVEY MICROLENSING PLANET DETECTIONS

Yee, J. C.,Shvartzvald, Y.,Gal-Yam, A.,Bond, I. A.,Udalski, A.,Kozłowski, S.,Han, C.,Gould, A.,Skowron, J.,Suzuki, D.,Abe, F.,Bennett, D. P.,Botzler, C. S.,Chote, P.,Freeman, M.,Fukui, A.,Furusawa, K. IOP Publishing 2012 The Astrophysical journal Vol.755 No.2

<P>Because of the development of large-format, wide-field cameras, microlensing surveys are now able to monitor millions of stars with sufficient cadence to detect planets. These new discoveries will span the full range of significance levels including planetary signals too small to be distinguished from the noise. At present, we do not understand where the threshold is for detecting planets. MOA-2011-BLG-293Lb is the first planet to be published from the new surveys, and it also has substantial follow-up observations. This planet is robustly detected in survey+follow-up data (Delta chi(2) similar to 5400). The planet/host mass ratio is q = (5.3 similar to 0.2) x 10(-3). The best-fit projected separation is s = 0.548 +/- 0.005 Einstein radii. However, due to the s <-> s(-1) degeneracy, projected separations of s-1 are only marginally disfavored at Delta chi(2) = 3. A Bayesian estimate of the host mass gives M-L = 0.43(-0.17)(+0.27) M-circle dot, with a sharp upper limit of M-L < 1.2 M-circle dot from upper limits on the lens flux. Hence, the planet mass is m(p) = 2.4(-0.9)(+1.5) M-Jup, and the physical projected separation is either r(perpendicular to) similar or equal to 1.0 AU or r(perpendicular to) similar or equal to 3.4 AU. We show that survey data alone predict this solution and are able to characterize the planet, but the Delta chi(2) is much smaller (Delta chi(2) similar to 500) than with the follow-up data. The Delta chi(2) for the survey data alone is smaller than for any other securely detected planet. This event suggests a means to probe the detection threshold, by analyzing a large sample of events like MOA-2011-BLG-293, which have both follow-up data and high-cadence survey data, to provide a guide for the interpretation of pure survey microlensing data.</P>

DISCOVERY AND MASS MEASUREMENTS OF A COLD, 10 EARTH MASS PLANET AND ITS HOST STAR

Muraki, Y.,Han, C.,Bennett, D. P.,Suzuki, D.,Monard, L. A. G.,Street, R.,Jorgensen, U. G.,Kundurthy, P.,Skowron, J.,Becker, A. C.,Albrow, M. D.,Fouqué,, P.,Heyrovský,, D.,Barry, R. K.,Beau IOP Publishing 2011 The Astrophysical journal Vol.741 No.1

<P>We present the discovery and mass measurement of the cold, low-mass planet MOA-2009-BLG-266Lb, performed with the gravitational microlensing method. This planet has a mass of m(p) = 10.4 +/- 1.7 M-circle plus and orbits a star of mass M-star = 0.56 +/- 0.09 M-circle dot at a semimajor axis of a = 3.2(-0.5)(+1.9) AU and an orbital period of P = 7.6(-1.5)(+7.7) yrs. The planet and host star mass measurements are enabled by the measurement of the microlensing parallax effect, which is seen primarily in the light curve distortion due to the orbital motion of the Earth. But the analysis also demonstrates the capability to measure the microlensing parallax with the Deep Impact (or EPOXI) spacecraft in a heliocentric orbit. The planet mass and orbital distance are similar to predictions for the critical core mass needed to accrete a substantial gaseous envelope, and thus may indicate that this planet is a 'failed' gas giant. This and future microlensing detections will test planet formation theory predictions regarding the prevalence and masses of such planets.</P>

On the Superconducting Symmetry of Fe-based Systems - Impurity Effect Studies and Neutron Scattering Measurements-

Masatoshi Sato,Yoshiaki Kobayashi,Takayuki Kawamata,Yukio Yasui,Kazunori Suzuki,M. Itoh,Ryoichi Kajimoto,Kazuhiko Ikeuchi,M. Arai,Phillipe Bourges 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.12

To identify the superconducting symmetry of Fe-based superconductors, we studied effects ofnonmagnetic-impurities on Tc, magnetic excitation spectra 00 and NMR 1/T1 − T curve, whichare sensitive to the relative signs between the order parameters on the disconnected Fermi surfacesin reciprocal space, because the symmetry is closely connected with the pairing mechanism:If the signs are opposite (symmetry S±), the mechanism is considered to be a magneticone, but when the signs are the same (symmetry S++), a novel mechanism is plausible becausethe ordinary phonon mechanism cannot realize the Tc value as high as 55 K found in Ln1111(Ln=lanthanide). Results are as follows: (a) The very small rates of Tc-suppression by impurityatoms M of LnFe1−yMyAs0.89−xF0.11+x (M=Ni, Co, Ru) can be explained only by S++. (b) The00-data for Ba(Fe, Co)2As2 (Tc 23 K) and Ca-Fe-Pt-As crystals seem to be well explained byS++ rather than by S±. (c) The nuclear magnetic resonance data can be consistently understoodby S++, too. These results suggest the S++ symmetry and a novel pairing mechanism, which canbe considered to be related to the elastic softening of C66 induced by the orbital fluctuation of thesystem.

Ultra-trace analysis of plutonium by thermal ionization mass spectrometry with a continuous heating technique without chemical separation

Lee, C.G.,Suzuki, D.,Esaka, F.,Magara, M.,Song, K. Pergamon Press ; Elsevier Science Ltd 2015 Talanta Vol.141 No.-

Thermal ionization mass spectrometry (TIMS) with a continuous heating technique is known as an effective method for measuring the isotope ratio in trace amounts of uranium. In this study, the analytical performance of thermal ionization mass spectrometry with a continuous heating technique was investigated using a standard plutonium solution (SRM 947). The influence of the heating rate of the evaporation filament on the precision and accuracy of the isotope ratios was examined using a plutonium solution sample at the fg level. Changing the heating rate of the evaporation filament on samples ranging from 0.1fg to 1000fg revealed that the influence of the heating rate on the precision and accuracy of the isotope ratios was slight around the heating rate range of 100-250mA/min. All of the isotope ratios of plutonium (SRM 947), <SUP>238</SUP>Pu/<SUP>239</SUP>Pu, <SUP>240</SUP>Pu/<SUP>239</SUP>Pu, <SUP>241</SUP>Pu/<SUP>239</SUP>Pu and <SUP>242</SUP>Pu/<SUP>239</SUP>Pu, were measured down to sample amounts of 70fg. The ratio of <SUP>240</SUP>Pu/<SUP>239</SUP>Pu was measured down to a sample amount of 0.1fg, which corresponds to a PuO<SUB>2</SUB> particle with a diameter of 0.2μm. Moreover, the signals of <SUP>239</SUP>Pu could be detected with a sample amount of 0.03fg, which corresponds to the detection limit of <SUP>239</SUP>Pu of 0.006fg as estimated by the 3-sigma criterion. <SUP>238</SUP>Pu and <SUP>238</SUP>U were clearly distinguished owing to the difference in the evaporation temperature between <SUP>238</SUP>Pu and <SUP>238</SUP>U. In addition, <SUP>241</SUP>Pu and <SUP>241</SUP>Am formed by the decay of <SUP>241</SUP>Pu can be discriminated owing to the difference in the evaporation temperature. As a result, the ratios of <SUP>238</SUP>Pu/<SUP>239</SUP>Pu and <SUP>241</SUP>Pu/<SUP>239</SUP>Pu as well as <SUP>240</SUP>Pu/<SUP>239</SUP>Pu and <SUP>242</SUP>Pu/<SUP>239</SUP>Pu in plutonium samples could be measured by TIMS with a continuous heating technique and without any chemical separation processes.

A surface sensitive optical method for the evaluation of processed ZnO:exploitation of LO phonon interaction

C.Harada,H.Makino,H.Goto,T.Minegishi,T.Suzuki,M.W.Cho,T.Yao 한국물리학회 2004 Current Applied Physics Vol.4 No.6

We report on a surface property of bulk ZnO crystals and an optical method to evaluate it. Bulk ZnO crystals have a damagedsurface layer due to chemomechanical polishing. We prepared the ZnO crystals by etching, and evaluated the improvement of thesurface by high-resolution X-ray diraction (XRD) and photoluminescence (PL). In PL measurements, the relative intensity of therst order longitudinal optical phonon replica of free exciton (FX-1LO) to second order process (FX-2LO) was compared. Therelative intensity becomes weak with increasing etched depth and nally saturates at the etched depth of 5l m. This result agrees wellwith XRD results.

OGLE-2012-BLG-0563Lb: A SATURN-MASS PLANET AROUND AN M DWARF WITH THE MASS CONSTRAINED BY<i>SUBARU</i>AO IMAGING

Fukui, A.,Gould, A.,Sumi, T.,Bennett, D. P.,Bond, I. A.,Han, C.,Suzuki, D.,Beaulieu, J.-P.,Batista, V.,Udalski, A.,Street, R. A.,Tsapras, Y.,Hundertmark, M.,Abe, F.,Bhattacharya, A.,Freeman, M.,Itow, IOP Publishing 2015 The Astrophysical journal Vol.809 No.1

<P>We report the discovery of a microlensing exoplanet OGLE-2012-BLG-0563Lb with the planet-star mass ratio of similar to 1 x 10(-3). Intensive photometric observations of a high-magnification microlensing event allow us to detect a clear signal of the planet. Although no parallax signal is detected in the light curve, we instead succeed at detecting the flux from the host star in high-resolution JHK'-band images obtained by the Subaru/AO188 and Infrared Camera and Spectrograph instruments, allowing us to constrain the absolute physical parameters of the planetary system. With the help of spectroscopic information about the source star obtained during the high-magnification state by Bensby et al., we find that the lens system is located at 1.3(-0.8)(+0.6) kpc from us, and consists of an M dwarf (0.34(-0.20)(+0.12)M(circle dot)) orbited by a Saturn-mass planet (0.39(-0.23)(+0.14)M(Jup)) at the projected separation of 0.74(-0.42)(+0.26)AU (close model) or 4.3(-2.5)(+1.5)AU (wide model). The probability of contamination in the host star's flux, which would reduce the masses by a factor of up to three, is estimated to be 17%. This possibility can be tested by future high-resolution imaging. We also estimate the (J - K-s) and (H - K-s) colors of the host star, which are marginally consistent with a low metallicity mid-to-early M dwarf, although further observations are required for the metallicity to be conclusive. This is the fifth sub-Jupiter-mass (0.2 < m(p)/M-Jup < 1) microlensing planet around an M dwarf with the mass well constrained. The relatively rich harvest of sub-Jupiters around M dwarfs is contrasted with a possible paucity of similar to 1-2 Jupiter-mass planets around the same type of star, which can be explained by the planetary formation process in the core-accretion scheme.</P>

PREGNANCY RATE AND SURVIVAL IN CULTURE OF IN VITRO FERTILIZED BOVINE EMBRYOS FROZEN IN VARIOUS CRYYOPROTECTANTS AND THAWED USING A ONE-STEP SYSTEM

T. Suzuki,M. Takagi,M. Yamamoto,A. Boediono,S. Saha,H. Sakakibara,M. Oe 한국수정란이식학회 1997 한국수정란이식학회 학술대회 Vol.1997 No.2

Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours(, 5% ) in modified TCM-199 medium supplemented with 5% superovulated cow serum(SCS) and inseminated by in vitro capacitated spermatozoa. Day 7 to 8 embryos were equilibrated for 10 minutes in 1.3M methyl cellosolve(MC) <1.1M diethylene glycol(DEG), 1.8M ethylene glycol(EG), 1.6M propylene glycol(PG) and 1.1 M 1,3-butylene glycol(BG) solutions. They were then loaded into 0.25ml straws, placed into an alcohol bath freezer at , cooled from to at /minute, seeded, held for 10 minutes, and stored in liquid nitrogen. After thawing in water, the embryos wee rehydrated in TCM-199 medium and then cultured for 48 hours in TCM-199 plus 5% SCS. Embryos were considered viable if they progressed to later developmental stages with a good morphology. Some of the embryos frozen in each cryoprotectant were thawed and transferred non-surgically without removing the cryoprotectant. Hatched embryos survived freezing and one-step dilution as follows : EG(50.0%), MC(53.6%), DEG(56.9%), PG(58.0%) and BG(11.5%). The survival rate of embryos cooled at vs. /minute was not significantly different(P<0.05), however, blastocysts hatched most often (P<0.01) in vitro when cooled at a rate of /minute(64.6%), 31/48) than at /minute(22.6%, 12/53). Pregnancy rates resulting from embryos frozen in the different cryoprotectants were as follows : MC(48%, 10/21); DEG(30%, 3/10); EG(74%, 20/27); and PG(40%, 4/10). These results indicate that MC, DEG, EG and PG have utility as cryoprotectants for the freezing and thawing of IVF Bovine embryos.

Coulomb-interaction effect on the two-dimensional electronic structure of the van der Waals ferromagnet Cr2Ge2Te6

Suzuki, M.,Gao, B.,Koshiishi, K.,Nakata, S.,Hagiwara, K.,Lin, C.,Wan, Y. X.,Kumigashira, H.,Ono, K.,Kang, Sungmo,Kang, Seungjin,Yu, J.,Kobayashi, M.,Cheong, S.-W.,Fujimori, A. American Physical Society 2019 Physical Review B Vol.99 No.16