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현병근(Byung-Keun Hyun),정석재(Sug-Jae Jung),손연규(Yeon-Kyu Sonn),박찬원(Chan-Won Park),장용선(Young-Seon Zhang),송관철(Kwan-Cheol Song),김이현(Lee-Hyun Kim),최은영(Eun-Young Choi),홍석영(Suk-Young Hong),권순익(Sun-Ik Kwon),장병춘(B 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.5
산채류 주산단지인 강원 평창, 홍천, 횡성, 양구 등 4개 지역 53농가포장을 중심으로 실시한 세부정밀토양 조사 결과에 의해 밝혀진 토성, 배수등급, 유효토심, 지형, 경사, 자갈함량, 해발 등의 토양특성과 산채류 수량을 통하여 작물재배적지기준 설정방법인 최대저해인자법과 다변량분석법의 두 가지 방법을 비교분석하였다. 산채류재배지의 수량과 토양의 형태 및 물리적특성을 비교하여 보면 토성 (미사)식양질, 유효토심은 >100 cm, 경사는 2~15%, 지형은 곡간 및 선상지, 해발 500 m이상인 토양에서 수량이 가장 높았다. 토양특성과 산채류 수량에 미치는 기여도산출결과 경사 0.30, 해발 0.22,지형 0.13, 배수등급 0.09 등 순으로 나타났다. 강원도 홍천군을 대상으로 최대저해인자법을 이용하여 산채류재배적지기준을 적용한 결과 최적지 0.2%, 적지 15.0%, 가능지 16.7% 그리고 저위생산지 68.0%로 나타났다. 그러나, 다변량분석법을 이용하여 산채류재배적지기준을 적용한 결과 최적지 35.1%, 적지 30.7%, 가능지 10.3%, 저위생산지 23.9%로 나타나 두 방법간에 많은 차이가 있었다. 따라서, 작물재배적지기준을 설정할 때 분석방법을 충분히 고려하여 기준을 설정할 필요가 있고, 앞으로 작물재배적지기준설정시 다변량 분석에 의한 방법을 적극 활용하는 것이 좋을 것으로 판단된다. The objective of this study was analysis of two methods of land suitability classification for wild edible green. One method was Maximum limiting factor method (MLFM) and the other was Multi-regression method (MRM) for land suitability classification for wild edible green. The investigation was carried out in Pyeongchang, Hongcheong, Hoeingseong, and Yanggu regions in Korea. The obtained results showed that factors related to the decision classification of the land suitability for wild edible green cultivation were land slope, altitude, soil morphology and gravel contents so on. The classification of the best suitability soil for wild edible greens were fine loamy (silty), valley or fan of soil morphology, well drainage class, B-slope (2~7%), available soil depth deeper than 100cm, and altitude higher than 501m. Contribution of soil that influence to crop yields using Multi-regression method were slope 0.30, altitude 0.22, soil morphology 0.13, drainage classes 0.09, available soil depth 0.07, and soil texture 0.01 orders. Using MLFM, area of best suitable land was 0.2%, suitable soil 15.0%, possible soil 16.7%, and low productive soil 68.0% in Hongcheon region of Gangwon province. But, area of best suitable land was 35.1%, suitable soil 30.7%, possible soil 10.3%, and low productive soil 23.9% by MRM. There was big difference of suitable soil area between two methods (MLFM and MRM). When decision classificatin of the land suitability for wild edible green cultivation should consider enough analysis methods. Furthermore, to establishment of land suitability classification for crop would be better use MRM than MLFM.
Biocompatible Peritoneal Dialysis Solution Preserves Residual Renal Function
Kim, Sejoong,Oh, Kook-Hwan,Oh, Jieun,Kim, Soo Jin,Chung, Wookyung,Song, Young Rim,Na, Ki Young,Oh, Yun Kyu,Ahn, Curie,Kim, Sung Gyun,Tan, Kathryn C.B. S. Karger AG 2012 American journal of nephrology Vol.36 No.4
<P>Abstract</P><P><B><I>Background/Aims:</I></B> The long-term effects of biocompatible peritoneal dialysis (PD) solution on residual renal function (RRF), inflammation, adipokines and metabolic acidosis are controversial. We evaluated the effects of biocompatible PD solution in continuous ambulatory PD (CAPD) patients for an additional 12-month period. <B><I>Method:</I></B> Among 91 incident patients who started CAPD with either biocompatible PD solution (Balance®, Fresenius; LS, n = 48) or conventional PD solution (CAPD/DPCA®, Fresenius; CS, n = 43), 63 patients, who were followed for 12 months, were enrolled and followed for an additional 12 months. <B><I>Results:</I></B> After 24 months of treatment, the glomerular filtration rate (GFR) of the LS group was twofold higher compared to the CS group (33.5 ± 30.7 vs. 16.3 ± 17.9 l/week/1.73 m<SUP>2</SUP>, respectively, p <I>= </I>0.021). In a subgroup of patients with an initial GFR >2 ml/min/1.73 m<SUP>2</SUP>, the GFR of the LS group was significantly higher than the rate of the CS group after 24 months (43.7 ± 30.5 vs. 18.6 ± 19.0 l/week/1.73 m<SUP>2</SUP>, respectively, p = 0.042). Over a 24-month period, effluent cancer antigen-125 levels were significantly increased in the LS group compared to the CS group, while effluent interleukin-6 levels did not differ between the two groups. The serum tCO<SUB>2</SUB> levels were consistently higher in the LS group compared to the CS group. <B><I>Conclusions:</I></B> We found that the effect of LS on preserving RRF may be maintained over a 24-month treatment period in CAPD patients, and LS use may have other benefits, such as the correction of metabolic acidosis.</P><P>Copyright © 2012 S. Karger AG, Basel</P>
Oxidative Stress Interferes With White Matter Renewal After Prolonged Cerebral Hypoperfusion in Mice
Miyamoto, Nobukazu,Maki, Takakuni,Pham, Loc-Duyen D.,Hayakawa, Kazuhide,Seo, Ji Hae,Mandeville, Emiri T.,Mandeville, Joseph B.,Kim, Kyu-Won,Lo, Eng H.,Arai, Ken American Heart Association, Inc. 2013 Stroke Vol.44 No.12
<P><B>Background and Purpose—</B></P><P>White matter injury caused by cerebral hypoperfusion may contribute to the pathophysiology of vascular dementia and stroke, but the underlying mechanisms remain to be fully defined. Here, we test the hypothesis that oxidative stress interferes with endogenous white matter repair by disrupting renewal processes mediated by oligodendrocyte precursor cells (OPCs).</P><P><B>Methods—</B></P><P>In vitro, primary rat OPCs were exposed to sublethal CoCl<SUB>2</SUB> for 7 days to induce prolonged chemical hypoxic stress. Then, OPC proliferation/differentiation was assessed. In vivo, prolonged cerebral hypoperfusion was induced by bilateral common carotid artery stenosis in mice. Then, reactive oxygen species production, myelin density, oligodendrocyte versus OPC counts, and cognitive function were evaluated. To block oxidative stress, OPCs and mice were treated with the radical scavenger edaravone.</P><P><B>Results—</B></P><P>Prolonged chemical hypoxic stress suppressed OPC differentiation in vitro. Radical scavenging with edaravone ameliorated these effects. After 28 days of cerebral hypoperfusion in vivo, reactive oxygen species levels were increased in damaged white matter, along with the suppression of OPC-to-oligodendrocyte differentiation and loss of myelin staining. Concomitantly, mice showed functional deficits in working memory. Radical scavenging with edaravone rescued OPC differentiation, ameliorated myelin loss, and restored working memory function.</P><P><B>Conclusions—</B></P><P>Our proof-of-concept study demonstrates that after prolonged cerebral hypoperfusion, oxidative stress interferes with white matter repair by disrupting OPC renewal mechanisms. Radical scavengers may provide a potential therapeutic approach for white matter injury in vascular dementia and stroke.</P>
Kim, Kyu-Il,Kayes, Terrence B.,Amundson, Clyde H. 濟州大學校 農科大學 動物科學硏究所 1992 動物科學論叢 Vol.7 No.1
Kim, K.I., Kayes, T.B. and Amundson, C.H., 1992. Requirements for sulfur amino acids and utiliza-tion of D-methionine by rainbow trout (Uncorhynchus mykiss). Aquacul~ure, 101 : 95-103. The methionine (Met) requirement and replacement values ofcystine (Cys) and D-Met for L-Met were estimated at 15℃ for fingerling rainbow trout (Uncorhynchus mykiss) by feeding diets contain-ing various levels of L-Met, D-Met and L-Cys. Four tanks, each containing 28 (Experiment Ⅰ) or 30 (Experiment 2 ) trout (11-15 g initial weight), were assigned to each diet. Weight gain and nitrogen retention during a 6-week period increased with increasing levels of L-Met up to 0.5% of diet, when the diets contained excess L-Cys (0.5%). Feed efficiency increased as L-Met increased. but no signif-icant (P>0.05) differences were detected at levels above 0.4%. With diets containing 0.5% L-Met, the Cys requirement was found to be 0.3% of the diet. Increasing D-Met level above 0.27% in a diet containing 0.23% L-Met did not significantly ( P<0.05) influence weight gain. feed eficiency or ni-trogen retention. Analysis of the results showed that l . the Met requirement of fingerling rainbow trout is 0.52 (I.49)% of diet (dietary protein). when a diet contains excess Cys; 2. the Cys replace-ment value (on an equal molar sulfur basis) of L-Cys for L-Met is approximately 42%: 3. the require-ment oftrout for total sulfuraminoacidsisabout 0.8 (2.3)%ofdiet (dietarypr0tein);and 4. rainbow trout utilize D-Met as efficiently as L-Met for weight gain but less efficiently for protein accretion.
Haar Cascade와 DNN 기반의 실시간 얼굴 표정 및 음성 감정 분석기 구현
유찬영(Chan-Young Yu),서덕규(Duck-Kyu Seo),정유철(Yuchul Jung) 한국컴퓨터정보학회 2021 한국컴퓨터정보학회 학술발표논문집 Vol.29 No.1
본 논문에서는 인간의 표정과 목소리를 기반으로 한 감정 분석기를 제안한다. 제안하는 분석기들은 수많은 인간의 표정 중 뚜렷한 특징을 가진 표정 7가지를 별도의 클래스로 구성하며, DNN 모델을 수정하여 사용하였다. 또한, 음성 데이터는 학습 데이터 증식을 위한 Data Augmentation을 하였으며, 학습 도중 과적합을 방지하기 위해 콜백 함수를 사용하여 가장 최적의 성능에 도달했을 때, Early-stop 되도록 설정했다. 제안하는 표정 감정 분석 모델의 학습 결과는 val loss값이 0.94, val accuracy 값은 0.66이고, 음성 감정 분석모델의 학습 결과는 val loss 결과값이 0.89, val accuracy 값은 0.65로, OpenCV 라이브러리를 사용한 모델 테스트는 안정적인 결과를 도출하였다.
Effects of Acteoside on Maturation and Development of Porcine Oocytes In Vitro
K. J. Kim,J. H. Lee,X. X. Li,E. Y. Kim,Y. B. Park,K. S. Park,Y. N. Ha,M. Kim,K. W. Han,J. Y. Park,J. Yu,D. S. Lee,Min Kyu Kim 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
Acteoside (verbascoside) is a typical phenylethanoid glycoside, extracted from various plants. It has various biological functions such as anti-oxidant, anti-inflammation, and anti-hypertension. Specially, it was powerful anti-oxidants either by direct scavenging of reactive oxygen and nitrogen species, or by acting as chain-breaking peroxyl radical scavengers. We examined the role of acteoside in IVM medium on the morphological progress of meiosis, developmental competence, and ROS in porcine oocytes. And we investigated effect of acteoside on the oocytes condition represented by cytoplasmic maturation by homogeneous distribution and formation of cytoplasmic organelles and regulation of apoptosis-related genes. The selected COCs were cultured in TCM-199 with various concentration of acteoside: 0 (control), 10, 30, and 50 μM. After 22 h of maturation with hormones, the oocytes were washed twice in a fresh maturation medium before being cultured in hormone-free medium for additional 22 h. The oocytes maturation rates of supplemented with acteoside were no significantly different compared with control group (71.13, 75.96, 72.95 and 73.68%, respectively). Level of ROS was significantly decreased in acteoside treated group. Furthermore, the parthenogenetic blastocyst rate was significantly improved in 10 μM acteoside treated group compared with control group (40.03 vs. 22.95%). During IVM, 10 μM acteoside treated oocytes showed that the mitochondria and lipid droplet were smaller and homogeneous distribution in cytoplasm compare with non-treated control oocytes. And reverse transcription polymerase chain reaction (RT-PCR) witarthenogenetic blstocysts revealed that acteoside increased the anti-apoptoticgenes, otherwise reibued pro-apoptotic genes. In conclusion, our results represents that addition of acteoside to the IVM medium has a beneficial effect in physiology of porcine oocytes such as viability and activation, providing a improved method for porcine oocytes in vitro.