배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

Achieving High Performance Ambipolar MoS₂ One-Dimensional Electrical Contact FET Through Plasma Etching

Zheng Yang,Won Jong Yoo 한국표면공학회 2017 한국표면공학회 학술발표회 초록집 Vol.2017 No.11

Improvement of the Spectral Reconstruction Process with Pretreatment of Matrix in Convex Optimization

Zheng-shuai Jiang,Xin-yang Zhao,WEI HUANG,Tao Yang 한국광학회 2021 Current Optics and Photonics Vol.5 No.3

In this paper, a pretreatment method for a matrix in convex optimization is proposed to optimize the spectral reconstruction process of a disordered dispersion spectrometer. Unlike the reconstruction process of traditional spectrometers using Fourier transforms, the reconstruction process of disordered dispersion spectrometers involves solving a large-scale matrix equation. However, since the matrices in the matrix equation are obtained through measurement, they contain uncertainties due to out of band signals, background noise, rounding errors, temperature variations and so on. It is difficult to solve such a matrix equation by using ordinary nonstationary iterative methods, owing to instability problems. Although the smoothing Tikhonov regularization approach has the ability to approximatively solve the matrix equation and reconstruct most simple spectral shapes, it still suffers the limitations of reconstructing complex and irregular spectral shapes that are commonly used to distinguish different elements of detected targets with mixed substances by characteristic spectral peaks. Therefore, we propose a special pretreatment method for a matrix in convex optimization, which has been proved to be useful for reducing the condition number of matrices in the equation. In comparison with the reconstructed spectra gotten by the previous ordinary iterative method, the spectra obtained by the pretreatment method show obvious accuracy.

从《倩女幽魂》到《九层妖塔》

??(Yang Zheng) 중국영화포럼 2016 영화중국 Vol.3 No.1

Since the 1990s, Chinese culture and society resulted in many changes, Chinese Monster films had shown a history of promoting hidden behind the text in context. By combing the period from Ghost Story to Chronicles of the Ghostly Tribe, monster image’s changes in Chinese monster films, this paper argues that the Chinese monster film increasing emphasis on the story of the text and the ideological content of will become the new trend.

The effect of SiO2 on the performance of inorganic sludge-based structural concretes

Zheng Xun Yang,Na Ra Ha,이종국,Min Sik Jang,황규홍 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.3

As a relatively new material, geopolymer concrete offers benefits as a construction material for sustainable development. It utilizes waste materials such as recycled concrete sludge, fly ash, etc. It has a very low rate of green house gas emission when compared to ordinary Portland cement. In this study, the components of geopolymer are concrete sludge, metakaolin and water glass. NaOH was used as an alkaline activator. To improve the mechanical properties, silica fume was added as a bonding matrix from 0%-10% to replace part of the concrete sludge, and the specimens were cured in air, then their mechanical properties such as compressive strength and bending strength were measured and their microstructures were investigated.

Friendship Influence on Mobile Behavior of Location Based Social Network Users

Yang Song,Zheng Hu,Xiaoming Leng,Hui Tian,Kun Yang,Xin Ke 한국통신학회 2015 Journal of communications and networks Vol.17 No.2

In mobile computing research area, it is highly desirable to understand the characteristics of user movement so that the user friendly location aware services could be rendered effectively. Location based social networks (LBSNs) have flourished recently and are of great potential for movement behavior exploration and datadriven application design. While there have been some efforts on user check-in movement behavior in LBSNs, they lack comprehensive analysis of social influence on them. To this end, the socialspatial influence and social-temporal influence are analyzed synthetically in this paper based on the related information exposed in LBSNs. The check-in movement behaviors of users are found to be affected by their social friendships both from spatial and temporal dimensions. Furthermore, a probabilistic model of user mobile behavior is proposed, incorporating the comprehensive social influence model with extent personal preference model. The experimental results validate that our proposed model can improve prediction accuracy compared to the state-of-the-art social historical model considering temporal information (SHM+T), which mainly studies the temporal cyclic patterns and uses them to model user mobility, while being with affordable complexity.

Rate-based model for predicting and evaluating H2S absorption in the haloalkaliphilic biological desulfurization process

Zheng Chen,Gama Yang,Tingzhen Mu,Maohua Yang,Nadia A. Samak,Sumit Peh,Yunpu Jia,Xuemi Hao,Xuhao Zhao,Jianmin Xing 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

The highly efficient performance of H2S absorption is the crucial indicator for haloalkaliphilic biologicaldesulfurization (HBDS) because it immediately concerns the H2S removal efficiency and pH change ofalkaline solutions. Therefore, we investigated the effect of operating parameters on the H2S absorption’sperformance under haloalkaline conditions. The gas–liquid ratio and packing height significantly improveH2S removal efficiency, from 80% to 90% and 66% to 99%, respectively. The absorption temperature had atrivial impact on the H2S removal efficiency, and the maximum value appeared at 45 C. Additionally, alloperating parameters caused pH changes that varied in the acceptable range (0.1 to 0.5) during theabsorption process. A rate-based model was successfully developed to predict the haloalkaliphilic H2Sabsorption process accurately. Moreover, this model could be implemented to effectively evaluate theHBDS system’s stability and provide reliable theoretical guidance for the industrial HBDS process toensure good process stability.

Research on the Influence of Polarization Aberration on Heterodyne Efficiency in Space Coherent Laser Communication System

Yang Zheng,Yu Piao 한국광학회 2022 Current Optics and Photonics Vol.6 No.1

Heterodyne efficiency is an indicator to evaluate the performance of space coherent laser communication systems. It is affected by signal light and local oscillator (LO) light amplitude, phase and polarization state. In this paper, based on the common heterodyne efficiency, a heterodyne efficiency model that can reflect polarization aberration of optical system is proposed. The heterodyne efficiency is analyzed when the signal light and the LO light are linearly polarized or circularly polarized. For a coherent communication optical system, when the incident signal light is right-circularly polarized light and the incident LO light is 45° linear polarized light. Based on the three-dimensional ray tracing theory and the heterodyne efficiency proposed in this paper, the change of polarization states and the distribution of heterodyne efficiency of the signal light and LO light influenced by the optical system’s polarization aberration are analyzed. Analysis shows that the heterodyne efficiency model proposed in this paper can be used to evaluate coherent communication systems and reflect the influence of optical system polarization aberration.

Icariside II Promotes the Differentiation of Adipose Tissue-Derived Stem Cells to Schwann Cells to Preserve Erectile Function after Cavernous Nerve Injury

Zheng, Tao,Zhang, Tian-biao,Wang, Chao-liang,Zhang, Wei-xing,Jia, Dong-hui,Yang, Fan,Sun, Yang-yang,Ding, Xiao-ju,Wang, Rui Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6

Icariside II (ICA II) is used in erectile dysfunction treatment. Adipose tissue-derived stem cells (ADSCs) are efficient at improving erectile function. This study aimed to explore the action mechanism of ADSCs in improving erectile function. ADSCs were isolated from the adipose tissues of rats. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay. The expressions of mRNA and protein were determined separately through qRT-PCR and western blot. The endogenous expressions of related genes were regulated using recombinant plasmids and cell transfection. A Dual-Luciferase Reporter Assay was performed to determine the interaction between miR-34a and STAT3. Rat models with bilateral cavernous nerve injuries (BCNIs) were used to assess erectile function through the detection of mean arterial pressure (MAP) and intracavernosal pressure (ICP). ICA II promoted ADSCs' proliferation and differentiation to Schwann cells (SCs) through the inhibition of miR-34a. Suppressed miR-34a promoted the differentiation of ADSCs to SCs by upregulating STAT3. ICA II promoted the differentiation of ADSCs to SCs through the miR-34a/STAT3 pathway. The combination of ICA II and ADSCs preserved the erectile function of the BCNI model rats. ADSCs treated with ICA II markedly preserved the erectile function of the BCNI model rats, which was reversed through miR-34a overexpression. ICA II promotes the differentiation of ADSCs to SCs through the miR34a/STAT3 pathway, contributing to erectile function preservation after the occurrence of a cavernous nerve injury.

Applying Artificial Neural Network to Fault Diagnosis of Analogue Circuit

Yang, ZhEng Rong,Musgrave, G. 대한전자공학회 1993 ICVC : International Conference on VLSI and CAD Vol.3 No.1

In this paper, we have applied artificial neural networks to fault diagnosis of analogue circuits. The experiments presented have proved that the artificial neural network can be used both for catastrophic faults and parametric faults. We have applied three artificial neural network, models to the fault diagnosis of DC, frequency, parametric, and steady state response. Two important techniques are introduced for the selection of artificial neural network models. These two techniques are: pattern distinction and quantity factor graph.