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Zhao, Rong Jie,Zhao, Zheng Lin,Zhao, Xiu Feng,Zhao, Guang Wen,Li, Meng Quan,Wu, Yi Yan,Li, Jing Qiu,Guan, Li Xin,Kim, Sang-Chan The Korean Medicine Society for the Herbal Formula 2009 大韓韓醫學方劑學會誌 Vol.17 No.2
The effects of aqueous extract of Schizandrae Fructus (AESC) on lead (Pb)-induced changes of monoamine neurotransmitters in the hippocampus (HIP) of adult rats were investigated. Male Sprague-Dawley rats were received intraperitoneal (i.p.) administration of Pb acetate (5 mg/kg/d) for 28 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in HIP were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Pb treated rats (P < 0.05), while pretreatment with AESC (100 mg/kg/d or 300 mg/kg/d, p.o., 2 h before Pb) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC (300 mg/kg/d) significantly increased the reduction of glutathione contents and superoxide dismutase activities in HIP induced by chronic Pb. These results suggest that AESC ameliorates Pb-induced depletion of monoamine neurotransmitters in HIP through its antioxidant activity.
Zhao, Li-Wei,Zhong, Xiu-Hong,Yang, Shu-Yan,Zhang, Yi-Zhong,Yang, Ning-Jiang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7
Inonotus obliquus is a medicinal mushroom that has been used as an effective agent to treat various diseases such as diabetes, tuberculosis and cancer. Inotodiol, an included triterpenoid shows significant anti-tumor effect. However, the mechanisms have not been well documented. In this study, we aimed to explore the effect of inotodiol on proliferation and apoptosis in human cervical cancer HeLa cells and investigated the underlying molecular mechanisms. HeLa cells were treated with different concentrations of inotodiol. The MTT assay was used to evaluate cell proliferating ability, flow cytometry (FCM) was employed for cell cycle analysis and cell apoptosis, while expression of cyclinE, p27, bcl-2 and bax was detected by immunocytochemistry. Proliferation of HeLa cells was inhibited by inotodiolin a dose-dependent manner at 24h (r=0.9999, p<0.01). A sub-$G_1$ peak (apoptotic cells) of HeLa cells was detected after treatment and the apoptosis rate with the concentration and longer incubation time (r=1.0, p<0.01), while the percentage of cells in S phase and $G_2$/M phase decreased significantly. Immunocytochemistry assay showed that the expression of cyclin E and bcl-2 in the treated cells significantly decreased, while the expression of p27 and bax obviously increased, compared with the control group (p<0.05). The results of our research indicate that inotodiol isolated from Inonotus obliquus inhibited the proliferation of HeLa cells and induced apoptosis in vitro. The mechanisms may be related to promoting apoptosis through increasing the expression of bax and cutting bcl-2 and affecting the cell cycle by down-regulation the expression of cyclin E and up-regulation of p27. The results further indicate the potential value of inotodiol for treatment of human cervical cancer.
( Yan-jun Wang ),( Xiu-qiong Lang ),( Dan Wu ),( Yu-qin He ),( Chun-hui Lan ),( Xiao-xiao ),( Bin Wang ),( Duo-wu Zou ),( Ji-min Wu ),( Yong-bin Zhao ),( Peter W Dettmar ),( Dong-feng Chen ),( Min Yan 대한소화기 기능성질환·운동학회 2020 Journal of Neurogastroenterology and Motility (JNM Vol.26 No.1
Background/Aims To determine the value of salivary pepsin in discriminating sub-types of gastroesophageal reflux disease (GERD) and GERD-related disorders. Methods Overall, 322 patients with different sub-types of GERD and 45 healthy controls (HC) were studied. All patients took Gastroesophageal Reflux Disease Questionnaire (GerdQ) and underwent endoscopy and 24-hour esophageal pH monitoring and manometry. Salivary pepsin concentration (SPC) was detected by using colloidal gold double-antibody immunological sandwich assay. Oral esomeprazole treatment was administrated in the patients with non-erosive reflux disease (NERD) and extra-esophageal symptoms (EES). Results Compared to HC, patients with erosive esophagitis, NERD, EES, EES plus typical GERD symptoms, or Barrett’s esophagus had a higher prevalence of saliva and SPC (all P < 0.001). There was no significant difference in the positive rate for pepsin in patients with functional heartburn or GERD with anxiety and depression, compared to HC. After esomeprazole treatment, the positive rate and SPC were significantly reduced in NERD (both P < 0.001) and in EES (P = 0.001 and P = 0.002, respectively). Of the 64 NERD patients, 71.9% (n = 46) were positive for salivary pepsin, which was significantly higher than the rate (43.8%, n = 28) of pathological acid reflux as detected by 24-hour esophageal pH monitoring (P = 0.002). Conclusions Salivary pepsin has an important significance for the diagnosis of GERD and GERD-related disorders. Salivary pepsin and 24-hour esophageal pH monitoring may complement with each other to improve the diagnostic efficiency.
Meng Zhao,Gui-Xin Wang,Xiu-Li Li,Rui Liu,Kang-Ping Yan 대한금속·재료학회 2010 METALS AND MATERIALS International Vol.16 No.6
Microscale Fe-P alloy was prepared using Ca3(PO4)2 and Fe2O3 in an electrothermal reduction process, and the electrochemical performance was investigated in detail. The initial discharge capacity could reach ~566.8mAh/g at 0.3 C rate, and the fade trend was so slight that the normalized capacities from 1.0 to 2.0 C rate were adjacent. The energy density and the power density could reach ~1133.6 Wh/kg and ~912.4 W/kg,respectively. The rate capability and the cycle performance were comparable to those of the Fe-P alloy synthesized using zerovalent iron and phosphorus. At 0.5 mV/s scan rate, the oxidation peak and the reduction peak for the reaction of lithium ions with P were positioned at ~1.3 and ~0.5 V, respectively. The reaction occurred under diffusion control, and the lithium ion diffusion efficient was ~1.5×10−9 cm2/s.
( Ming Yi Zhao ),( Ming Hua Yang ),( Liang Chun Yang ),( Yan Yu ),( Min Xie ),( Shan Zhu ),( Rui Kang ),( Dao Lin Tang ),( Zhi Gang Jiang ),( Wu Zhou Yuan ),( Xiu Shan Wu ),( Li Zhi Cao ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9
HMGB1 is associated with human cancers and is an activator of autophagy which mediates chemotherapy resistance. We here show that the mRNA levels of HMGB1 are high in leukemia cells and it is involved in the progression of childhood chronic myeloid leukemia (CML). HMGB1 decreases the sensitivity of human myeloid leukemia cells K562 to anti-cancer drug induced death through up-regulating the autophagy pathway, which is confirmed by the observation with an increase in fusion of autophagosomes and autophagolysosomes. When overexpressing HMGB1, both mRNA levels of Beclin-1, VSP34 and UVRAG which are key genes involved in mammalian autophagy and protein levels of p-Bcl-2 and LC3-II are increased. Luciferase assays document that over-expression of HMGB1 increases the transcriptional activity of JNK and ERK, which may be silenced by siRNA. The results suggest that HMGB1 regulates JNK and ERK required for autophagy, which provides a potential drug target for therapeutic interventions in childhood CML. [BMB reports 2011; 44(9): 601-606]
Overexpression of ENA1 from Yeast Increases Salt Tolerance in Arabidopsis
( Xiang Qiang Kong ),( Xiu Hua Gao ),( Wei Huan Li ),( Ji Qiang Zhao ),( Yan Xiu Zhao ),( Hui Zhang ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.2
In yeast, the plasma membrane Na+/H+ antiporter and Na+ -ATPase are key enzymes for salt tolerance. Saccharomyces cerevisiae Na+ -ATPase (Ena1p ATPase) is encoded by the ENA1/PMR2A gene; expression of ENA1 is tightly regulated by Na+ and depends on ambient pH. Although Ena1p is active mainly at alkaline pH values in S. cerevisiae, no Na+ -ATPase has been found in flowering plants. To test whether this yeast enzyme would improve salt tolerance in plants, we introduced ENA1 into Arabidopsis (cv. Columbia) under the control of the cauliflower mosaic virus 35S promoter. Transformants were selected for their ability to grow on a medium containing kanamyin. Southern blot analyses confirmed that ENA1 was transferred into the Arabidopsis genome and northern blot analyses showed that ENA1 was expressed in the transformants. Several transgenic homozygous lines and wild-type (WT) plants were evaluated for salt tolerance. No obvious morphological or developmental differences existed between the transgenic and WT plants in the absence of stress. However, overexpression of ENA1 in Arabidopsis improved seed germination rates and salt tolerance in seedlings. Under saline conditions, transgenic plants accumulated a lower amount of Na+ than did the wild type, and fresh and dry weights of the former were higher. Other experiments revealed that expression of ENA1 promoted salt tolerance in transgenic Arabidopsis under both acidic and alkaline conditions.
Synthesis of rod-like titanium doped hydroxyapatite nanopowder
Li-li Wang,Xiu-feng Wang,Cheng-long Yu,Yan-qi Zhao 한양대학교 세라믹연구소 2013 Journal of Ceramic Processing Research Vol.14 No.6
Rod-like titanium doped hydroxyapatite (Ti-doped HA) nanopowder was synthesized using Ca(NO3)2 • 4H2O, (NH4)2HPO4 and tetraethylorthotitanate as precursor materials via a combination of chemical co-precipitation and microwave techniques route. The effect of the content of titanium in Ti-doped HA (0.2-2.4 wt.%) on sintering behavior, crystal size, shape and morphology was investigated. The results suggested that the addition of titanium to HA structure did restrain HA decomposition and thermal reaction between HA and titanium, which resulted in improved thermal stability of HA at high sintering temperature. The grain size of sintered Ti-doped HA, which was much smaller than that of pure HA. 0.8 wt.% addition of titanium to HA, was optimum for producing nanometer-sized rod-like Ti-doped HA crystal with improved thermal stability.
Rong Jie Zha,Zheng Lin Zhao,Xiu Feng Zhao,Guang Wen Zha,Meng Quan Li,Yi Yan Wu,Jing Qiu Li,Li Xin Guan,김상찬 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2
The effects of aqueous extract of Schizandrae Fructus (AESC) on lead (Pb)-induced changes of monoamine neurotransmitters in the hippocampus (HIP) of adult rats were investigated. Male Sprague-Dawley rats were received intraperitoneal (i.p.) administration of Pb acetate (5 mg/kg/d) for 28 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in HIP were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Pb treated rats (P < 0.05), while pretreatment with AESC (100 mg/kg/d or 300 mg/kg/d, p.o., 2 h before Pb) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC (300 mg/kg/d) significantly increased the reduction of glutathione contents and superoxide dismutase activities in HIP induced by chronic Pb. These results suggest that AESC ameliorates Pb-induced depletion of monoamine neurotransmitters in HIP through its antioxidant activity.
Cloning and expression of a Chk1 gene in Daphnia pulex during different modes of reproduction
Xiaoge Guo,Shanliang Xu,Xuemei Yan,Wei Zhou,Xinyu Dai,Xiu Zou,Chunlin Wang,Danli Wang,Yunlong Zhao 한국유전학회 2015 Genes & Genomics Vol.37 No.9
The full-length cDNA of a Chk1 gene (DpChk1) was cloned from Daphnia pulex using RACE method. This cDNA is 1767 bp in length, consisting of a 1497 bp open reading frame encoding 498 amino acid protein. The deduced DpChk1 protein sequence contains phosphorylation sites, three conserved Ser-Gln and Thr- Gln motifs and shares 51–55 % similarity with A. pisum, M. rotundata, D. melanogaster, A. echinatior, B. terrestris, R. pulchellus and M. occidentalis, respectively. Gene expression analysis showed that Chk1 is differentially expressed in D. pulex during different reproductive modes. DpChk1 expression in sexually-reproducing females and males is significantly higher than in parthenogenetic Daphnia. Whole-mount in situ hybridization revealed that DpChk1 was expressed during two kinds of reproductive modes. In parthenogenetic females, hybridization signals were found in the antennae and thoracic limb, whereas expression levels in the corresponding sites of sexuallyreproducing females were relatively strong, DpChk1 could not be detected in the gonads of ephippial and parthenogenetic females. Taken together, these different reproductive stages and sex specific expression patterns are regulated temporally and spatially. We speculate that DpChk1 may be involved in conversion between different stages of reproduction and in sexual differentiation in D. pulex.