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Cloning and expression of a Chk1 gene in Daphnia pulex during different modes of reproduction
Xiaoge Guo,Shanliang Xu,Xuemei Yan,Wei Zhou,Xinyu Dai,Xiu Zou,Chunlin Wang,Danli Wang,Yunlong Zhao 한국유전학회 2015 Genes & Genomics Vol.37 No.9
The full-length cDNA of a Chk1 gene (DpChk1) was cloned from Daphnia pulex using RACE method. This cDNA is 1767 bp in length, consisting of a 1497 bp open reading frame encoding 498 amino acid protein. The deduced DpChk1 protein sequence contains phosphorylation sites, three conserved Ser-Gln and Thr- Gln motifs and shares 51–55 % similarity with A. pisum, M. rotundata, D. melanogaster, A. echinatior, B. terrestris, R. pulchellus and M. occidentalis, respectively. Gene expression analysis showed that Chk1 is differentially expressed in D. pulex during different reproductive modes. DpChk1 expression in sexually-reproducing females and males is significantly higher than in parthenogenetic Daphnia. Whole-mount in situ hybridization revealed that DpChk1 was expressed during two kinds of reproductive modes. In parthenogenetic females, hybridization signals were found in the antennae and thoracic limb, whereas expression levels in the corresponding sites of sexuallyreproducing females were relatively strong, DpChk1 could not be detected in the gonads of ephippial and parthenogenetic females. Taken together, these different reproductive stages and sex specific expression patterns are regulated temporally and spatially. We speculate that DpChk1 may be involved in conversion between different stages of reproduction and in sexual differentiation in D. pulex.
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Optimal sensing period in cooperative relay cognitive radio networks
Zhang, Shibing,Guo, Xin,Zhang, Xiaoge,Qiu, Gongan Korean Society for Internet Information 2016 KSII Transactions on Internet and Information Syst Vol.10 No.12
Cognitive radio is an efficient technique to improve spectrum efficiency and relieve the pressure of spectrum resources. In this paper, we investigate the spectrum sensing period in cooperative relay cognitive radio networks; analyze the relationship between the available capacity and the signal-to-noise ratio of the received signal of second users, the target probability of detection and the active probability of primary users. Finally, we derive the closed form expression of the optimal spectrum sensing period in terms of maximum throughput. We simulate the probability of false alarm and available capacity of cognitive radio networks and compare optimal spectrum sensing period scheme with fixed sensing period one in these performance. Simulation results show that the optimal sensing period makes the cognitive networks achieve the higher throughput and better spectrum sensing performance than the fixed sensing period does. Cooperative relay cognitive radio networks with optimal spectrum sensing period can achieve the high capacity and steady probability of false alarm in different target probability of detection. It provides a valuable reference for choosing the optimal spectrum sensing period in cooperative relay cognitive radio networks.
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Optimal sensing period in cooperative relay cognitive radio networks
( Shibing Zhang ),( Xin Guo ),( Xiaoge Zhang ),( Gongan Qiu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.12
Cognitive radio is an efficient technique to improve spectrum efficiency and relieve the pressure of spectrum resources. In this paper, we investigate the spectrum sensing period in cooperative relay cognitive radio networks; analyze the relationship between the available capacity and the signal-to-noise ratio of the received signal of second users, the target probability of detection and the active probability of primary users. Finally, we derive the closed form expression of the optimal spectrum sensing period in terms of maximum throughput. We simulate the probability of false alarm and available capacity of cognitive radio networks and compare optimal spectrum sensing period scheme with fixed sensing period one in these performance. Simulation results show that the optimal sensing period makes the cognitive networks achieve the higher throughput and better spectrum sensing performance than the fixed sensing period does. Cooperative relay cognitive radio networks with optimal spectrum sensing period can achieve the high capacity and steady probability of false alarm in different target probability of detection. It provides a valuable reference for choosing the optimal spectrum sensing period in cooperative relay cognitive radio networks.
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