Extraskeletal Ewing Sarcomas in Late Adolescence and Adults: A Study of 37 Patients

Tao, Hai-Tao,Hu, Yi,Wang, Jin-Liang,Cheng, Yao,Zhang, Xin,Wang, Huan,Zhang, Su-Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Background: Extraskeletal Ewing sarcoma (EES)/primitive neuroectodermal tumours (PNET) are rare soft tissue sarcomas. Prognostic factors and optimal therapy are still unconfirmed. Materials and Methods: We performed a retrospective analysis on patients to explore the clinic characteristics and prognostic factors of this rare disease. A total of 37 patients older than 15 years referred to our institute from Jan., 2002 to Jan., 2012 were reviewed. The characteristics, treatment and outcome were collected and analyzed. Results: The median age was 28 years (range 15-65); the median size of primary tumours was 8.2 cm (range 2-19). Sixteen patients (43%) had metastatic disease at the initial presentation. Wide surgical margins were achieved in 14 cases (38%). Anthracycline or platinum-based chemotherapy was performed on 29 patients (74%). Radiotherapy was delivered in 13 (35%). At a median follow-up visit of 24 months (range 2-81), the media event-free survival (EFS) and overall survival (OS) were 15.8 and 30.2 months, respectively. The 3-year EFS and OS rates were 24% and 43%, respectively. Metastases at presentation and wide surgical margins were significantly associated with OS and EFS. Tumour size was significantly associated with OS but not EFS. There were no significant differences between anthracycline and platinum based chemotherapy regarding EFS and OS. Conclusions: EES/PNET is a malignant tumour with high recurrence and frequent distant metastasis. Multimodality therapy featuring wide surgical margins, aggressive chemotherapy and adjuvant local radiotherapy is necessary for this rare disease. Platinum-based chemotherapy can be used as an adjuvant therapy.

An Improved Method for Rapid Preparation of Oligodendrocytespecific Rabbit Polyclonal Antibody

Tao Wang,Wei Wang,Lintao Jia,Xin Bu,Fang Wang,Libo Yao,Bin Zhang 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3

Antibodies are important tools in the study of protein function and diagnostic tests. However, traditional antiserum preparation requires a time-consuming immunization protocol and subsequent purification of polyclonal antibodies. In this study, a rapid and efficient method for polyclonal antibody preparation has been developed. Juxtanodin (JN) and silent information regulator-2 (Sirt2),both of which are oligodendrocyte-specific proteins, were used for antibody preparation. The N-terminal 170 amino acids of JN (JN170) and amino acids 231-351 of Sirt2 (Sirt2-121) were expressed as GST-tagged proteins from a pET-41a(+) vector in E. coli strain BL21 (DE3) cells. The fusion proteins were purified and used to immunize rabbits following both a traditional protocol, in which antigen was presented biweekly, and a modified rapid protocol, in which the immunization on day 1 was boosted on days 5and 28. ELISA, Western blot analysis and immunofluorescent staining showed that antibodies produced via the rapid protocol could recognize these two oligodendrocytespecific proteins in vitro and in the rat central nervous system (CNS), respectively, similar to those produced with the traditional protocol. Thus, our study provides a novel rapid method to prepare high specificity antibodies via a modified immunization protocol and subsequent antibody purification.

Development and Validation of Nomograms to Provide Individualized Predictions of Sur-vival Benefits from Surgery in Patients with Intermediate/Advanced Hepatocel-lular Carcinoma

( Wen-tao Yan ),( Jia-he Wang ),( Ming-da Wang ),( Zheng Wang ),( Bing Quan ),( Ya-hao Zhou ),( Wei-min Gu ),( Hong Wang ),( Ting-hao Chen ),( Tian Yang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: According to the BCLC treatment guidelines, surgery does not be recommended for intermediate/advanced hepatocellular carcinoma (HCC). In real world, however, liver resections are often performed in patients with intermediate/ advanced but resectable HCC, especially in the East. Methods: We retrospectively evaluated multicentric data of 1,325 patients newly diagnosed with intermediate/advanced HCC who underwent curative resection. We randomly divided the subjects into development (n = 875) and validation (n = 450) samples. Multivariate Cox proportional hazards models were developed and separately validated on the basis of patients’ clinicopathological variables assessed for associations with 1-year recurrence and 3-year mortality. The discriminatory accuracy of these models was compared with conventional tools by analyzing receiver operating characteristic (ROC) curves. Results: He statistical nomograms built based on performance status, Child-Pugh grade, portal hypertension, preoperative alpha-fetoprotein level, tumor rupture, largest tumor diameter, tumor number, macrovascular and microvascular invasion, and satellites had good calibration and discriminatory abilities, with c-indices of 0.70 (1-year recurrence) and 0.68 (3-year survival), respectively. These models showed satisfactory goodness-of-fit and discrimination abilities in the validation cohort (c-index, 0.68 for 1-year recurrence and 0.69 for 3-year survival). The areas under the ROC curve using these nomograms exceeded those of traditional staging systems, indicating superior discriminatory capability (c-indices, 0.60-0.63 and 0.56-0.62, respectively). Conclusions: Our proposed online nomograms, which present graphically postoperative prognostic models for recurrence and survival in patients with intermediate/advanced but resectable HCC, offer valuable guidance to surgeons and hepatologists for individually predicting survival benefits from surgery and planning recurrence surveillance and adjuvant therapy.

Classicality in Imaginary Weak Amplification

Tao Wang,Gang Li,Chang-Bao Fu,Rui Zhang,Xuemei Su,Qian-qian Bao 한국물리학회 2017 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.71 No.12

Weak ampli¯cation is an interesting phenomenon. In this paper we show an example that the imaginary weak ampli¯cation e®ect can be only related to the classical probability distribution of the measured quantity. A general result for any two orthogonal postselections and any meter state is also given.

Stochastic analysis for uncertain deformation of foundations in permafrost regions

Wang, Tao,Zhou, Guoqing,Wang, Jianzhou,Zhao, Xiaodong,Yin, Leijian Techno-Press 2018 Geomechanics & engineering Vol.14 No.6

For foundations in permafrost regions, the displacement characteristics are uncertain because of the randomness of temperature characteristics and mechanical parameters, which make the structural system have an unexpected deviation and unpredictability. It will affect the safety of design and construction. In this paper, we consider the randomness of temperature characteristics and mechanical parameters. A stochastic analysis model for the uncertain displacement characteristic of foundations is presented, and the stochastic coupling program is compiled by Matrix Laboratory (MATLAB) software. The stochastic displacement fields of an embankment in a permafrost region are obtained and analyzed by Neumann stochastic finite element method (NSFEM). The results provide a new way to predict the deformation characteristics of foundations in permafrost regions, and it shows that the stochastic temperature has a different influence on the stochastic lateral displacement and vertical displacement. Construction disturbance and climate warming lead to three different stages for the mean settlement of characteristic points. For the stochastic settlement characteristic, the standard deviation increases with time, which imply that the results of conventional deterministic analysis may be far from the true value. These results can improve our understanding of the stochastic deformation fields of embankments and provide a theoretical basis for engineering reliability analysis and design in permafrost regions.

Prognostic Value of MGMT Promoter Methylation and TP53 Mutation in Glioblastomas Depends on IDH1 Mutation

Wang, Kai,Wang, Yin-Yan,Ma, Jun,Wang, Jiang-Fei,Li, Shao-Wu,Jiang, Tao,Dai, Jian-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Several molecular markers have been proposed as predictors of outcome in patients with glioblastomas. We investigated the prognostic significance of $O^6$-methylguanine-DNA methyltransferase (MGMT) promoter methylation and TP53 mutation status dependent on isocitrate dehydrogenase 1 (IDH1) mutation in glioblastoma patients. A cohort of 78 patients with histologically confirmed glioblastomas treated with radiation therapy and chemotherapy were reviewed retrospectively. We evaluated the prognostic value of MGMT promoter methylation and TP53 mutation status with regard to progression-free survival (PFS) and overall survival (OS). It was revealed that mutations in IDH1, promoter methylation of MGMT, TP53 mutation, age, Karnofsky performance status (KFS), and extension of resection were independent prognostic factors. In patients with an IDH1 mutation, those with an MGMT methylation were associated with longer PFS (p=0.016) and OS (p=0.013). Nevertheless, the presence of TP53 mutation could stratify the PFS and OS of patients with IDH1 wild type (p=0.003 and 0.029 respectively, log-rank). The MGMT promoter methylation and TP53 mutation were associated with a favorable outcome of patients with and without mutant IDH1, respectively. The results indicate that glioblastomas with MGMT methylation or TP53 mutations have improved survival that may be influenced by IDH1 mutation status.

Pharmacokinetic Behavior of Gentiopicroside From Decoction of Radix Gentianae, Gentiana Macrophylla After Oral Administration in Rats: a Pharmacokinetic Comaprison with Gentiopicroside after Oral and Intravenous Administration Alone

Wang, Chang-Hong,Cheng, Xue-Mei,He, Yu-Qi,White, Kenneth N.,Bligh, S.W.Annie,Branford-White, Christopher J.,Wang, Zheng-Tao 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.9

The pharmacokinetics in rats of gentiopicroside (GPS) from orally administered decoctions of Radix Gentianae (DRG) and Gentiana macrophlla (DGM) were compared with that of GPS alone administered at 150 mg/kg orally and 30 mg/kg intravenously. The metabolic profile of GPS after intravenous injection could be fitted to two-compartment model whereas oral administration decoctions DRG or DGM, or GPS alone, could all be fitted to a one-compartment model. After oral administration of GPS alone, GPS was absorbed quickly and reached a maximum plasma concentration ($C_{max}$) value, 5.78 ${\pm}$ 2.24 ${\mu}g/mL$ within 0.75 ${\pm}$ 0.62 h. The plasma level of GPS declined with a $T_{1/2ke}$, 3.35 ${\pm}$ 0.76 h. After oral administration of decoctions DRG and DGM, GPS was absorbed and reached significantly higher maximum concentrations of 10.53 ${\pm}$ 3.20 ${\mu}g/mL$ (p < 0.01) and 7.43 ${\pm}$ 1.64 ${\mu}g/mL$ (p < 0.05) at later time points 1.60 ${\pm}$ 0.76 (p < 0.01) and 2.08${\pm}$0.74 h (p < 0.05), for DRG and DGM respectively, compared with oral GPS alone. Significantly larger AUC values were found for decoctions of GPS (83.49 ${\pm}$ 20.8 ${\mu}g{\cdot}h/mL$ for DRG and 59.43 ${\pm}$ 12.9 ${\mu}g{\cdot}h/mL$ for DGM) compared with oral GPS alone (32.67 ${\pm}$ 12.9 ${\mu}g{\cdot}h/mL$. The results demonstrate that the bioavailability of GPS was markedly improved when administered as a decoction than as purified GPS. The decoction from Radix Gentianae provided 2.5 times better bioavailability and that from Gentiana macrophlla 1.8 times higher. The study confirms the importance of careful pharmacokinetic analysis in the characterization of herbal medicines when applied for future clinical applications.

Fault Diagnosis of Transformer Based on Self-powered RFID Sensor Tag and Improved HHT

Tao Wang,Yigang He,Bing Li,Tiancheng Shi 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.5

This work introduces a fault diagnosis method for transformer based on self-powered radio frequency identification (RFID) sensor tag and improved Hilbert-Huang transform (HHT). Consisted by RFID tag chip, power management circuit, MCU and accelerometer, the developed RFID sensor tag is used to acquire and wirelessly transmit the vibration signal. A customized power management including solar panel, low dropout (LDO) voltage regulator, supercapacitor and corresponding charging circuit is presented to guarantee constant DC power for the sensor tag. An improved band restricted empirical mode decomposition (BREMD) which is optimized by quantumbehaved particle swarm optimization (QPSO) algorithm is proposed to deal with the raw vibration signal. Compared with traditional methods, this improved BREMD method shows great superiority in reducing mode aliasing. Then, a promising fault diagnosis approach on the basis of Hilbert marginal spectrum variations is brought up. The measured results show that the presented power management circuit can generate 2.5V DC voltage for the rest of the sensor tag. The developed sensor tag can achieve a reliable communication distance of 17.8m in the test environment. Furthermore, the measurement results indicate the promising performance of fault diagnosis for transformer.

Degrees of Freedom of 3-user MIMO Interference Channels with Instantaneous Relay Using Interference Alignment

( Wang Qiang ),( Shu Yuquan ),( Dong Minhua ),( Xu Ji ),( Tao Xiaofeng ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.5

Instantaneous relay (relay-without-delay) using interference alignment is a promising approach to neutralizing interference and improving system capacity. In Wang Chenwei`s work, a 2-user scenario required both source and relay to access the global channel state information (CSI). This paper shows a new method of interference alignment improves the degrees of freedom (DoF) prominently for the 3-user MIMO interference channel with instantaneous relay. This new method is focused on the relay node that completes the alignment interference neutralization so the global CSI is obtained only once and the pressure on the base station can be mitigated. In addition, the 3-user MIMO interference channels with instantaneous relay can achieve 2M DoF when source and destination have M antennas, respectively. This method shows 33% improvement over the conventional method using interference alignment which obtains 3M/2 DoF.

Fault Diagnosis of Transformer Based on Self-powered RFID Sensor Tag and Improved HHT

Wang, Tao,He, Yigang,Li, Bing,Shi, Tiancheng The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.5

This work introduces a fault diagnosis method for transformer based on self-powered radio frequency identification (RFID) sensor tag and improved Hilbert-Huang transform (HHT). Consisted by RFID tag chip, power management circuit, MCU and accelerometer, the developed RFID sensor tag is used to acquire and wirelessly transmit the vibration signal. A customized power management including solar panel, low dropout (LDO) voltage regulator, supercapacitor and corresponding charging circuit is presented to guarantee constant DC power for the sensor tag. An improved band restricted empirical mode decomposition (BREMD) which is optimized by quantum-behaved particle swarm optimization (QPSO) algorithm is proposed to deal with the raw vibration signal. Compared with traditional methods, this improved BREMD method shows great superiority in reducing mode aliasing. Then, a promising fault diagnosis approach on the basis of Hilbert marginal spectrum variations is brought up. The measured results show that the presented power management circuit can generate 2.5V DC voltage for the rest of the sensor tag. The developed sensor tag can achieve a reliable communication distance of 17.8m in the test environment. Furthermore, the measurement results indicate the promising performance of fault diagnosis for transformer.