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- 저자 : ( Natarajan Sakthivel ) (검색결과 5 건)
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베드리칸난,Natarajan Sakthivel,한성수 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4
Metal- and carbon-based nanoparticles (MBNPs/CBNPs) with different nanometric dimensions have unique combinations of physical and chemical properties. The physico-chemical properties, morphology, and interactions of nanomaterials with biological systems differ according to the source of synthesis and the extent and type of functionalization involved, which have many advantages for exciting nanotechnological applications in the fields of chemical and biological sciences. The biosynthesis and biofabrication of nanomaterials are achieved in a myriad of ways using plants and microorganisms. Greener synthesis has been conducted using the extracts of plants or other biological entities. This review focuses on the recent insights in the green and biological synthesis of MBNPs based on cadmium, cerium, copper, gold, iron, manganese, nickel, palladium, platinum, selenium, silver, titanium, yttrium, zinc, and zirconium, and CBNPs, such as carbon dots (CDs), as well as their chemical and biological applications, such as chemical catalysis, anticancer, antioxidant, antimicrobial, antibiofilm, antidiabetic, insecticidal, scolicidal, antileshmanial, angiogenesis, and immunostimulant agents, and as tools for bioimaging, biosensing, and drug delivery.
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Biosynthesis of Silver Nanoparticles by Phytopathogen Xanthomonas oryzae pv. oryzae Strain BXO8
( Kannan Badri Narayanan ),( Natarajan Sakthivel ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9
Extracellular biogenic synthesis of silver nanoparticles with various shapes using the rice bacterial blight bacterium Xanthomonas oryzae pv. oryzae BXO8 is reported. The synthesized silver nanoparticles were characterized by UV-Vis spectroscopy, powder X-ray diffractometry (XRD), scanning electron microscopy, energy dispersive X-ray spectrometry, and highresolution transmission electron microscopy (HR-TEM). Based on the evidence of HR-TEM, the synthesized particles were found to be spherical, with anisotropic structures such as triangles and rods, with an average size of 14.86 nm. The crystalline nature of silver nanoparticles was evident from the bright circular spots in the SAED pattern, clear lattice fringes in the high-resolution TEM images, and peaks in the XRD pattern. The FTIR spectrum showed that biomolecules containing amide and carboxylate groups are involved in the reduction and stabilization of the silver nanoparticles. Using such a biological method for the synthesis of silver nanoparticles is a simple, viable, cost-effective, and environmentally friendly process, which can be used in antimicrobial therapy.
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Jayakumar Pathma,Niraikulam Ayyadurai,Natarajan Sakthivel 한국미생물학회 2010 The journal of microbiology Vol.48 No.6
Antagonistic fluorescent pseudomonads isolated from rice rhizospheric soil were characterized using biochemical, taxonomical and molecular tools. Production of cyclopropane fatty acid (CFA) was correlated with their antagonistic potential. Strains were grouped into 18 different genotypes on the basis of amplified ribosomal DNA restriction analysis (ARDRA) and repetitive (rep)-PCR based genotypic fingerprinting analyses. High phylogenetic resolution among antagonistic fluorescent pseudomonad strains was obtained based on the DNA gyrase B subunit (gyrB) and RNA polymerase sigma factor 70 (rpoD) gene sequence analyses. Combined gyrB and rpoD sequence analysis resulted in the accurate estimation of molecular phylogeny and provided a significant correlation between the genetic distances among strains. Present study demonstrated the genetic and functional relationship of fluorescent pseudomonads. The knowledge on genetic and functional potential of fluorescent pseudomonads associated with rice rhizosphere is useful to understand their ecological role and for their utilization in sustainable agriculture.
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Asha, Balachandrababu Malini,Revathi, Masilamani,Yadav, Amit,Sakthivel, Natarajan The Korean Society for Microbiology and Biotechnol 2012 Journal of microbiology and biotechnology Vol.22 No.11
A novel bacterial strain, MG7, with high cellulase activity was isolated and identified by morphological characteristics and molecular phylogeny analysis as Paenibacillus barcinonensis. Maximum production of cellulase by MG7 was observed at pH 7.0 and $35^{\circ}C$. The enzyme was purified with a specific activity of 16.88 U/mg, the cellulase activity was observed in a zymogram, and its molecular mass (58.6 kDa) was confirmed by SDS-PAGE. The purified enzyme showed maximum activity at pH 6.0 and $65^{\circ}C$ and degraded cellulosic substrates such as carboxy methyl cellulose (CMC), Avicel, filter paper, and ${\beta}$-glucan. The enzyme showed stability with 0.5% concentration of various surfactants. The $K_m$ and $V_{max}$ of cellulase for CMC and Avicel were found to be 0.459mg/ml and 10.46mg/ml/h, and 1.01 mg/ml and 10.0 mg/ml/h, respectively. The high catalytic activity and its stability to temperature, pH, surfactants, and metal ions indicated that the cellulase enzyme by MG7 is a good candidate for biotechnological applications.
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Medium constituents mediated engineering for size and shape tuning of gold nanocrystallites
Anil K. Suresh,Veena Vijay,Bahrudeen S. Hameed,Sureshkumar Ramasamy,Natarajan Sakthivel 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.51 No.-
The current investigation introduces the utilization of cell culture mediums as a novel source for the oneplotsynthesis as well as stabilization of metal nanoparticles. By varying the medium constituents wecould control the size and shape distributions of the gold nanoparticles. Nanospheres of diameter24 6 nm and 19 5 nm were produced using DMEM and M199 mediums respectively, nanoneedles of150 50 nm using RPMI medium and nanoflowers of 60 25 nm using IMDM medium, with an overallyield of 91 2%. A remarkable decrease in the reaction duration (<3 min) was noted, irrespective of thegrowth mediums used. Fourier transform infrared spectroscopy and zeta potential measurementsrevealed them to have a common protenacious encapping agent with different overall surface charges of23 3,211,24 2, and20 1 mV for Au@DMEM, Au@RPMI, Au@IMDM andAu@M199 respectively. X-ray diffraction confirmed the purity and crystallinity of the particles withBragg peaks at (111), (200), (220) and (311) for gold nanocrystals. This approach could lead to the creativeutilization of novel eco-friendly sources for the production and size/shape control of nanoparticles. Moreover, the adopted methodology is absolutely green, robust and facile, offering new insights forsustainable synthesis for various biomedical and engineering applications.
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