MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Zhang, Lei-Lei,Wu, Jiang,Liu, Qiang,Zhang, Yan,Sun, Zhu-Lei,Jing, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

Melatonin Attenuates Noise Stress-induced Gastrointestinal Motility Disorder and Gastric Stress Ulcer: Role of Gastrointestinal Hormones and Oxidative Stress in Rats

( Lei Zhang ),( Ji T Gong ),( Hu Q Zhang ),( Quan H Song ),( Guang H Xu ),( Lei Cai ),( Xiao D Tang ),( Hai F Zhang ),( Fang E Liu ),( Zhan S Jia ),( Hong W Zhang ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.2

Background/Aims There are increasing evidences for gastrointestinal motility disorder (GIMD) and gastric stress ulcer induced by noise stress. The present study was to investigate the reversed effect of melatonin on GIMD and gastric stress ulcer induced by noise stress and potential mechanism. Methods Noise stress was induced on rats, and melatonin (15 mg/kg) was administered to rats by intraperitoneal injection. Differences were assessed in gastric residual rate (GRR), small intestine propulsion rate (SPR), Guth injury score, cortisol, gastrointestinal hormones (calcitonin-gene-related peptide and motilin) and oxidative stress markers (superoxide dismutase and malondialde hyde) in blood plasma as well as gastric mucosa homogenate with or without melatonin. The pathological examination of gastric mucosa was also performed. Results The GRR and SPR were improved by noise stress compared with control (P < 0.05). The pathological examination and Guth injury score revealed gastric stress ulcer. Moreover, the levels of cortisol, motilin and malondialdehyde in blood plasma and malondialdehyde in gastric mucosa homogenate were increased by noise stress (P < 0.05). CGRP and superoxide dismutase activity in both of blood plasma and gastric mucosa homogenate were significantly decreased (P< 0.05). Furthermore, melatonin reversed changes in GRR, SPR, pathological examination, Guth injury score, cortisol, motilin, CGRP, superoxide dismutase activity and malondialdehyde (P < 0.05). Conclusions Melatonin is effective in reversing the GIMD and gastric stress ulcer induced by noise stress. The underlying mechanism may be involved in oxidative stress and gastrointestinal hormones. (J Neurogastroenterol Motil 2015;21:189-199)

Anomalous magnetization jumps in granular Pb superconducting films

Zhang An-Lei,Jiang Wan-Yan,Chen Xing-Hong,Zhang Xiao-Ke,Lu Wen-Lai,Chen Fei,Feng Zhen-Jie,Cao Shi-Xun,Zhang Jin-Cang,Ge Jun-Yi 한국물리학회 2022 Current Applied Physics Vol.35 No.-

In granular superconductors, the grain boundaries are closely related to the vortex dynamics and the macroscopic superconducting properties. In our research, Pb films with different grain sizes were prepared by tuning the substrate temperature. With the grain size decreasing, Pb films are prone to feature the anomalous magnetization jumps in the M − T curves, while in the M − H curves flux avalanche happens. Both phenomena appear in the same region of the H − T phase diagram and thus are considered to have the same origin. The further theoretical analysis shows that with grain size decreasing the pinning mechanism evolves from a mixed δTc and δl pinning to the δl pinning mechanism. The results shed light on the study of pinning mechanism for granular superconductors and is beneficial to the potential application of manipulating vortex pinning by regulation of intrinsic defects.

Preparation and Characterization of a Novel Hyperbranched Poly(aryl ether ketone) Terminated with Cobalt Phthalocyanine to be Used for Oxidative Decomposition of 2,4,6-Trichlorophenol

Lei Lei,Yunhe Zhang,Xiujie Li,Jianxin Mu,Guibin Wang,Zhenhua Jiang,Shuling Zhang 한국고분자학회 2010 Macromolecular Research Vol.18 No.4

A novel hyperbranched poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-HPAEK)was prepared using 1,3,5-tris[4-(4-fluorobenzoyl) phenoxy] benzene (B3), as a ‘core’ molecule, 4,4'-(hexafluoroisopropylidene)diphenol (6F-BPA) as an A2 monomer, and cobalt phthalocyanine as an end-capping reagent. CoPc-THPAEK showed good solubility, thermal stability and catalytic activity in the oxidative decomposition of 2,4,6-trichlorophenol (TCP) under H2O2 or KHSO5. Moreover, the oxidative effect of KHSO5 was superior to that of H2O2,and the catalytic activity of CoPc-T-HPAEK was much higher than that of linear poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-LPAEK) and linear poly(aryl ether ketone) with a cobalt phthalocyanine pendant unit (CoPc-S-LPAEK).

Association between Polymorphisms of MSTN and MYF5 Genes and Growth Traits in Three Chinese Cattle Breeds

Zhang, R.F.,Chen, H.,Lei, C.Z.,Zhang, C.L.,Lan, X.Y.,Zhang, Y.D.,Zhang, H.J.,Bao, B.,Niu, H.,Wang, X.Z. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.12

The objective of this study was to assess the association of polymorphisms in MSTN and MYF5 genes with growth traits in three Chinese cattle breeds. Only one homozygous animal with BB genotype at MSTN locus was observed in Jiaxian population which was at Hardy-Weinberg disequilibrium (p<0.05). The frequencies of allele A at MSTN locus and allele B at MYF5 locus in the three Chinese breeds were 0.9550/0.9730/0.9720 and 0.8275/0.7581/0.7523, respectively. Allele A at MSTN locus and allele B at MYF5 locus were dominant in these three populations. No statistically significant differences in growth traits were observed between the genotypes of the Jiaxian breed at MSTN and MYF5 loci and the Nanyang breed at MYF5 locus. However, there were statistically significant differences between the genotypes at MSTN locus of the Nanyang breed for WH, HG, HGI and HGBLR (p<0.05), and of the Qinchuan breed for BLI (p<0.05). The SNP in MYF5 had significant effects on WH and HHC of Qinchuan animals (p<0.05). These results suggest that MSTN and MYF5 are strong candidate genes that influence growth traits in cattle. Other SNPs of MSTN and MYF5 or other linked genes should also be studied, which could lead to the development of selection plans to improve the performance of Chinese cattle and also promote the breeding of genuine beef cattle in China.

Chinese Society of Allergy Guidelines for Diagnosis and Treatment of Allergic Rhinitis

Lei Cheng,Jianjun Chen,Qingling Fu,Shaoheng He,Huabin Li,Zheng Liu,Guolin Tan,Zezhang Tao,Dehui Wang,Weiping Wen,Rui Xu,Yu Xu,Qintai Yang,Chonghua Zhang,Gehua Zhang,Ruxin Zhang,Yuan Zhang,Bing Zhou,Do 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

Allergic rhinitis (AR) is a global health problem that causes major illnesses and disabilities worldwide. Epidemiologic studies have demonstrated that the prevalence of AR has increased progressively over the last few decades in more developed countries and currently affects up to 40% of the population worldwide. Likewise, a rising trend of AR has also been observed over the last 2-3 decades in developing countries including China, with the prevalence of AR varying widely in these countries. A survey of self-reported AR over a 6-year period in the general Chinese adult population reported that the standardized prevalence of adult AR increased from 11.1% in 2005 to 17.6% in 2011. An increasing number of original articles and imporclinical trials on the epidemiology, pathophysiologic mechanisms, diagnosis, management and comorbidities of AR in Chinese subjects have been published in international peer-reviewed journals over the past 2 decades, and substantially added to our understanding of this disease as a global problem. Although guidelines for the diagnosis and treatment of AR in Chinese subjects have also been published, they have not been translated into English and therefore not generally accessible for reference to non-Chinese speaking international medical communities. Moreover, methods for the diagnosis and treatment of AR in China have not been standardized entirely and some patients are still treated according to regional preferences. Thus, the present guidelines have been developed by the Chinese Society of Allergy to be accessible to both national and international medical communities involved in the management of AR patients. These guidelines have been prepared in line with existing international guidelines to provide evidence-based recommendations for the diagnosis and management of AR in China.

Annealing Effects on CuInS2 Thin Films Grown on Glass Substrates by Using Pulsed Laser Deposition

Lei Zhang,Jingang Fang,Mingkai Li,Xunzhong Shang,Yunbin He,강태원 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.3

CuInS2 thin films have been deposited on glass substrates at low temperature by using a self-madeceramic target and the pulsed laser deposition method. Polycrystalline and near-stoichiometricCuInS2 films with a band gap energy of 1.45 eV are obtained by post-annealing/sulfurization ata temperature of 500 C. X-ray diffraction reveals a highly (112) preferential orientation of theCuInS2 thin films while the Raman spectrum indicates the coexistence of a CuInS2 chalcopyritephase and CuAu orderings. A polymorphic transformation of metastable CuAu ordering into theequilibrium chalcopyrite structure takes place at a post-annealing temperature of 500 C with theCuxS phase being segregated at the surface. Further, the morphologies and the chemical statesof the films before and after annealing are characterized by atomic force microscopy and X-rayphotoelectron spectroscopy, respectively.

Adaptive Truncation technique for Constrained Multi-Objective Optimization

( Lei Zhang ),( Xiaojun Bi ),( Yanjiao Wang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.11

The performance of evolutionary algorithms can be seriously weakened when constraints limit the feasible region of the search space. In this paper we present a constrained multi-objective optimization algorithm based on adaptive ε-truncation (ε-T-CMOA) to further improve distribution and convergence of the obtained solutions. First of all, as a novel constraint handling technique, ε-truncation technique keeps an effective balance between feasible solutions and infeasible solutions by permitting some excellent infeasible solutions with good objective value and low constraint violation to take part in the evolution, so diversity is improved, and convergence is also coordinated. Next, an exponential variation is introduced after differential mutation and crossover to boost the local exploitation ability. At last, the improved crowding density method only selects some Pareto solutions and near solutions to join in calculation, thus it can evaluate the distribution more accurately. The comparative results with other state-of-the-art algorithms show that ε-T-CMOA is more diverse than the other algorithms and it gains better in terms of convergence in some extent.

A Novel Kinetic Analysis of Crystallization of Polypropylene (PP) in Dynamically-vulcanized PP/Ethylene-propylene-diene Rubber (EPDM) Blends Using an In-situ Measurement Technique

Lei Hu,Bin Yang,Yan-Li Deng,Fei-Xue Lu,Ru Xia,Zheng-Zhi Zheng,Ji-Bin Miao,Jia-Sheng Qian,Chuan-Ru Zhang,Peng Chen,Yu-Chuan Zhang 한국고분자학회 2017 폴리머 Vol.41 No.4

The effects of cooling medium temperatures and plastic/rubber ratios on solidification and crystallization kinetics of dynamically-vulcanized polypropylene/ethylene-propylene-diene rubber (PP/EPDM) blends were investigated with the aid of an in-situ measurement technique. The cooling medium temperature heavily influenced the solidification kinetics primarily due to a combination of latent heat liberated from the molten polymer and the heat transferred away via the metallic wall during the cooling period. Interestingly, the parameter C in three-parameter model was not only affected by the material properties, but also by the cooling condition, different from the previous literature. The crystallization kinetics analysis indicated that the effect of EPDM in the blends consisted of both nucleation-promoting effect (low EPDM loading) and steric effect (higher EPDM loading). The present kinetic analysis may be helpful to further studies on improving the product performances for industrial applications.

A Review of Li-ion Battery Equivalent Circuit Models

Zhang, Xiaoqiang,Zhang, Weiping,Lei, Geyang The Korean Institute of Electrical and Electronic 2016 Transactions on Electrical and Electronic Material Vol.17 No.6

Batteries are critical components of electric vehicles and energy storage systems. The connection of a battery to the power grid for charge and discharge greatly affects energy storage. Therefore, an accurate and easy-to-observe battery model should be established to achieve systematic design, simulation, and SOC (state of charge) estimations. In this review, several equivalent circuit models of representative significance are explained, and their respective advantages and disadvantages are compared to determine and outline their reasonable applications to Li-ion batteries. Numerous commonly used model parameter identification principles are summarized as well, and basic model verification methods are briefly introduced for the convenient use of such models.