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한우에 감염된 Theileria sergenti merozoite의 순수분리와 genomic DNA probe에 관한 연구
채준석,이주묵,권오덕,채건상 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from bovine crythrocytes. The infected erythrocytes were lysed by Aeromonas hydrophila(Ah-1) hemolysin, and the parasites were isolated by ultracentrifugation on a Percoll discontinuous density gradient. For construction of a T sergenti genomic DNA library, T sergenti DNA was digested with PstI and the fragments were ligated into the PstI site of pUC19 before transformation of Escherichia coli JM83. Out of thousands of transformants obtained by transformation of E coli JM83 with the genomic library, three plasmids were chosen. The sizes of the inserted DNAs were 2.9kb(2.4kb and 0.5kb) in pKTS1, 4.3kb in pKTS2 and 1.5kb in pKTS3, respectively. The DNA fragments used as probe KTS1(2.4kb), KTS2(4.3kb) and KTS3(1.5kb) were labeled digoxigenin-11-dUTP for the Southern hybridization. In Southern hybridization, all of the probes(KTS1, KTS2 and KTS3) reacted specifically to T sergenti DNA, but not to bovine leucocyte DNA. In order to find out the sensitivities of the digoxigenin-11-dUTP-labeled KTS1 and KTS3 as the probes, purified merozoite DNA and bovine DNA(control) were checked by dot blot hybridization with the probes. Both of the probes, KTS1 and KTS3, detected as minimum amount of 975pg of the T sergenti DNA, but not bovine DNA even to 500ng.
Si Bulk Micromachining을 위한 Wafer Rolling Etching 및 그 특성
김건년,이보나,박효덕,신상모,공경준,장동근,김병철,권혁채,이봉희 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1
A wafer rolling etching system for the silicon bulk micromachining has been designed and fabricated. The silicon diaphragms were anisotropically etched in a 24.5 weight percent KOH solution. Compared to the conventional KOH etching systems, pyramidal hillocks, and wave-shaped structures on the etched surfaces were greatly reduced by using this system. After etching for time of 438 minutes, the average etched depth and the etch-rate were measured to be 537μm and 1.22μm/min, respectively. The average etching uniformity of etching depth was 0.87% in 5-inch wafer. Our results showed that the wafer rolling method enhanced etch uniformity and etch rate.
Chromosomal Assignment of Short cDNA Sequences by PCR Using Overlapping and Tailed Short Primers
CHAE, Keon-Sang,MURAKAWA, Katsuji,OKUBO, Kousaku,MATSUBARA, Kenichi 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
Overlapping primers and tailed short primers are effective agents for mapping very short cDNA sequences. By using such primers, human cDNAs as short as 32 nucleotides in length can produce PCR bands. Using these and other primers of ordinary size, 44 cDNAs were assigned to chromosomes, of which 24 were assigned to single chromosomes, and 2 were assigned to two chromosomes and two were assigned to three chromosomes, respectively. Among the 24 cDNAs, all of which matched GenBank entries, 6 cDNAs were observed to map to the same chromosomes as reported previously.
Chae, Keon-Sang,Katsuji Murakawa,Kousaku Okubo,Kenichi Mastsubara 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
For the PCR-based chromosomal assignment of very short cDNA fragments specifically designed primers are required. We tested primers with very short core sequences that are identical or complementary to known cDNA sequences, with or without tails at the 5' ends. The lower limit of the core length for PCR using human chromosome templates was 14 nucleotides (nt) when they have tails. The minimal length of the tail was 2 nt when it was attached to the 5' end of a 14-nt core. In the absence of a tail. 15 nt are needed for the core to act properly. The overall size of the short cDNA fragments that could be assigned was further reduced by using a pair of primers that overlap at the 3' ends. The limits of the free energy of overlap were about -1.9 kcal mol at 45 C, -2.9 ㎉/mol at 50 C and -4.5 ㎉/mol at 55 C. A combination of these features in a primer pair allowed cDNA fragments as short as 30 nt to be assigned.
Proteus vulgaris로부터 N4 파아지에 대해 내성을 갖게 하는 유전자의 분리
채건상 전북대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.1 No.1
대장균에 감염하는 파아지 중의 하나인 N4가 대장균에 감염하지 못하도록 해주는 rtn(resistant to N4)유전자를 Proteus vulgaris로부터 대장균에 분리하였다. 이 rtn 유전자를 갖고 있는 재조합 플라스미드인 pRMG101을 갖고 있는 대장균은 전부 N4의 감염을 저해하였으나, 다른 파아지인 T4, T7, Ø80, lambda가 감염하는 데에는 아무 영향을 주지 못하였다. 이 유전자의 전체 염기 서열을 밝혀본 결과, promoter 부분과 두 개의 단백질을 만들 수 있는 open reading frame(rtn A와 rtn B)이 있다는 것을 알아내었는데 이 rtn 유전자의 promoter는 대장균내에서도 자신의 기능을 발휘하는 것이 확산되었다.
Sang-Chae Jeon,Keon Sik Kim,Dong-Joo Kim,Dong Seok Kim,Jong Hun Kim,Jihae Yoon,Jae Ho Yang 한국방사성폐기물학회 2019 방사성폐기물학회지 Vol.17 No.1
사고저항성 핵연료의 일환으로 UO2 입자가 세라믹 셀 벽으로 둘러싸인 미세구조를 갖는 세라믹 미소셀 UO2 소결체를 개발 중이다. 이는 핵분열생성물들을 UO2 펠렛 내에 포집하여 펠렛 외부로의 방출을 저감함으로써 봉내압 상승을 완화하고 응력부식균열 발생률을 낮춘다. 생성량이나 방사능 측면에서 위험한 핵분열생성물 중 하나로 여겨지는 세슘은 세라믹 미소셀 소결체 내에서 셀 물질과 화학반응 하여 포집될 수 있다. 따라서, 세슘 포집능은 해당 화학반응의 열역학적, 속도론적 특성에 의해 결정된다. 역으로, 미소셀 소결체의 조성설계 시 해당 반응에 대한 열역학적 예측이 필수적이다. 본 연구는 세라믹 현재 개발 중인 여러 미소셀 조성(Si-Ti-O, Si-Cr-O, Si-Al-O)에 대해 세슘의 포집능을 평가하는 열역학적 계산을 다룬다. 계 산에 앞서 먼저 HSC Chemistry를 이용해 세슘과 셀 물질의 물리/화학적 상태를 정의한 후, LWR 정상운전 모사환경에서 계 산된 세슘포텐셜(ΔGCs)과 산소포텐셜(ΔG02)에 근거하여 세슘포집 반응성을 평가하였다. 계산 결과에 근거하면, 세슘 포집 반응은 상기 모든 조성에서 자발적일 것으로 예상되며 이로써 조성설계의 근거를 제시함과 동시에 세슘의 포집능을 평가하는 효과적인 방법을 제공한다. As candidates for accident-tolerant fuels, ceramic microcell fuels, which are distinguished by their peculiar microstructures, are being developed; these fuels have UO2 grains surrounded by cell walls. They contribute to nuclear fuel safety by retention of fission products within the UO2 pellet, reducing rod pressure and incidence of SCC failure. Cesium, a hazardous fission product in terms of amount and radioactivity, can be captured by chemical reactions with ceramic cell materials. The capture-ability of cesium therefore depends on the thermodynamics of the capturing reaction. Conversely, compositional design of cell materials should be based on thermodynamic predictions. This study proposes thermodynamic calculations to evaluate the cesium capture-ability of three ceramic microcell compositions: Si-Ti-O, Si-Cr-O and Si-Al-O. Prior to the calculations, the chemical and physical states of the cesium and the cell materials were defined. Then, the reactivity was evaluated by calculating the cesium potential (ΔGCs) and oxygen potential (ΔG02) under simulated LWR circumstances of normal operation. Based on the results, cesium capture is expected to be spontaneous in all cell compositions, providing a basis for the compositional design of ceramic microcell fuels as well as a facile way for evaluating cesium capture.