A New Report of Biscogniauxia petrensis Isolated from Mosquitoes in Korea

( Kallol Das ),( Joung-ho Kim ),( Kwang-shik Choi ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.2

A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 μm (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as Biscogniauxia petrensis, new to Korea.

Taxonomy of Arthrinium minutisporum sp. nov., Pezicula neosporulosa, and Acrocalymma pterocarpi: New Records from Soil in Korea

( Kallol Das ),( Seung-yeol Lee ),( Hyo-won Choi ),( Ahn-heum Eom ),( Young-je Cho ),( Hee-young Jung ) 한국균학회 2020 Mycobiology Vol.48 No.6

The strains 17E-042, 17E-039, and NC13-171 belong to Ascomycota and were isolated from soil collected from Sancheong-gun and Yeongam-gun, Korea. The strain 17E-042 produced white mycelial colonies that developed a sienna color with a round margin on potato dextrose agar (PDA), and the reverse side developed a light sienna color. Morphologically, this strain was similar to the strains of Arthrinium phragmites and A. hydei, but the shorter conidial size of the newly identified strain (17E-042) was distinct. The strain 17E-039 produced macroconidia that were pale yellow to orange-brown, elongated-ellipsoid to oblong, round at both ends, primarily straight but sometimes slightly curved, 0-septate, thin-walled, and filled with numerous droplets, having diameters of 20.4-34.3×8.0-12.0 lm. And the strain NC13-171 formed hyaline to light brown chlamydospores, solitary or in a chain. Multigene phylogenetic analyses were conducted using sequence data obtained from internal transcribed spacer (ITS) regions, 28S rDNA large subunit (LSU), b-tubulin (TUB2), translation elongation factor 1-alpha (TEF1-a), and RNA polymerase II large subunit (RPB2) genes. The results of molecular phylogeny, the detailed descriptions and illustrations of each species strongly support our proposal that these strains from soil in Korea be designated as Arthrinium minutisporum sp. nov. and two new records of Pezicula neosporulosa and Acrocalymma pterocarpi.

Unreported Post-harvest Disease of Apples Caused by Plenodomus collinsoniae in Korea

( Kallol Das ),( Yeong-hwan Kim ),( Jingi Yoo ),( Leonid N. Ten ),( Sang-jae Kang ),( In-kyu Kang ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.4

This study was conducted to isolate and identify the fungal pathogen caused unreported post-harvest disease on apples (cv. Fuji) fruit in Korea. The disease symptoms on apples appeared as irregular, light to dark brown, slightly sunken spots. The three fungal strains were isolated from infected tissues of apple fruits and their cultural and morphological characteristics were completely consistent with those of Plenodomus collinsoniae. The phylogenetic analysis using the internal transcribed spacer (ITS) regions, beta-tubulin (TUB), and the second largest subunit of RNA polymerase II (RPB 2) sequences revealed the closest relationship of the isolates with Plenodomus collinsoniae at the species level. The pathogenicity test showed the same dark brown spots on Fuji apple cultivar. Therefore, P. collinsoniae is a newly reported fungal agent causing post-harvest disease on apples in Korea.

Molecular and Morphological Characterization of Two Novel Species Collected from Soil in Korea

( Kallol Das ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 Mycobiology Vol.48 No.1

Two fungal species of ascomycetes were discovered during the screening of soil microflora from the Gangwon Province in Korea: Didymella chlamydospora sp. nov. (YW23-14) and Microdochium salmonicolor sp. nov. (NC14-294). Morphologically, YW23-14 produces smaller chlamydospores (8.0-17.0×7.0-15.0 mm) than D. glomerata and D. musae. The strain NC14- 294 was characterized by smaller conidiogenous cells (4.9-8.8×2.0-3.2 mm) compared with the closest strains M. fisheri and M. phragmitis. The detailed descriptions, illustrations, and discussions regarding the morphological and phylogenetical analyses of the closely related species are provided to support the novelty of each species. Thus, YW23-14 and NC14-294 are described here as newly discovered species.

Pestalotiopsis kaki sp. nov., a Novel Species Isolated from Persimmon Tree (Diospyros kaki) Bark in Korea

( Kallol Das ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2021 Mycobiology Vol.49 No.1

During the screening of Korean microflora, a fungal strain (KNU-PT-1804) belonging to the genus Pestalotiopsis was isolated from persimmon tree (Diospyros kaki) bark collected from North Gyeongsang Province, Korea. The strain, KNU-PT-1804, produced smaller conidia compared with related species P. kenyana, P. neglecta, and P. telopeae. The novelty of the strain was confirmed based on phylogenetic analysis using molecular datasets of internal transcribed spacer (ITS) regions, b-tubulin (TUB2), and translation elongation factor 1-alpha (TEF1a) genes. Molecular phylogeny strongly supports that the strain is distinct from previously known Pestalotiopsis species, and we proposed the novel species, Pestalotiopsis kaki sp. nov., and provide a detailed description and illustration.

Molecular and Morphological Characterization of Three New Fungal Species of the Class Sordariomycetes from Korea

Das Kallol,반재호,Choi So-Young,Lee Seung-Yeol,정희영 한국균학회 2023 韓國菌學會誌 Vol.51 No.1

Three fungal strains belonging to the class Sordariomycetes were isolated from soil collected on Jeju Island and Gyeongsangbuk-do, Korea. They were identified as Diaporthe endophytica (KNU-JJ-1809), Faurelina indica (KNU-JJ-1830), and Trichoderma ivoriense (KNU-4-KH1). KNU-JJ-1809 produced beta conidia that were straight, curved, hyaline, smooth-walled, with a diameter of 16.5-25.0×0.6-1.7 μm. The conidia of strain KNU-JJ-1830 were hyaline to light green, thin, clavate, round, truncate base, had guttules at both ends, with a diameter of 2.5-5.2×1.7-3.8 μm. The conidia of strain KNU-4-KH1 were oblong or ellipsoidal, smooth-walled, greenish, with a diameter of 2.2-4.4×2.2-3.6 μm. Internal transcribed spacer regions, partial large subunit, translation elongation factor 1-alpha, β-tubulin, and calmodulin genes were used to confirm the strains, and their cultural and morphological characteristics. To our knowledge, this is the first report on D. endophytica, F. indica, and T. ivoriense in Korea.

Penicillifer diparietisporus: a New Record from Field Soil in Korea

( Kallol Das ),( Chang-gi Back ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.3

A fungus was isolated from field soil collected from Daegu, Korea. The colony of the isolated fungus showed short, branched, and light to dark yellow pigments with hyaline, yellowish red to orange brown aerial mycelia. In addition, the fungus produced solitary to aggregated perithecia, ovoid to pyriform, short neck, and asci as well as biseriately arranged ascospores. Phylogenetic analysis using the internal transcribed spacer region and translation elongation factor 1-α sequences and morphological characteristics identified the isolated fungus as Penicillifer diparietisporus , which belongs to the family Nectriaceae. To our knowledge, this is the first report of Penicillifer diparietisporus in Korea.

A New Record on Umbelopsis vinacea and Mucor hiemalis f. corticola Isolated from Korea

( Kallol Das ),( Ki-hun Ha ),( Sang-jae Suh ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.3

In the screening of fungal diversity, two strains were collected from the soil of Yeongcheon and dissected guts from the bodies of Chinese rice grasshopper (Oxya chinensis), Chinese grasshopper (Acrida cinerea), and Far eastern devil grasshopper (Oedaleus infernalis) from Daejeon in Korea. They were identified as Umbelopsis vinacea (KNU-YC-1801B) and Mucor hiemalis f. corticola (KNU-20F7, KNU-20F8, KNU-20F9). Multigene phylogenetic analyses of the internal transcribed spacer (ITS) regions, and large subunit (LSU) sequence data confirmed two unreported taxa along with their morphology. The results of molecular phylogeny firmly supported the detailed description and illustration for each taxon. As far as we know, both Umbelopsis vinacea and Mucor hiemalis f. corticola are the first reported taxa in Korea.

Molecular Phylogeny and Morphology of Tolypocladium globosum sp. nov. Isolated from Soil in Korea

Das Kallol,Ryu Jung-Joo,Hong Soo-Min,Lim Seong-Keun,Lee Seung-Yeol,Jung Hee-Young 한국균학회 2023 Mycobiology Vol.51 No.2

In this study, fungal strains designated as KNUF-22-14A and KNUF-22-15A were isolated from soil samples in Korea. These two strains were identified based on cultural and morphological characteristics as well as phylogenetic analyses and were found to be morphologically and phylogenetically identical. Upon their morphological comparison with closely related species, such as Tolypocladium album, T. amazonense, T. endophyticum, T. pustulatum, and T. tropicale, a difference in the size of short phialides [0.6–2.4(–9.3) × 0.8–1.4 µm] was observed. Meanwhile, these strains had larger conidia (1.2–3.0 × 1.2–3.0 µm) than T. album, T. amazonense, T. endophyticum, and T. tropicale and smaller conidia than T. pustulatum. Phylogenetic analyses using a multi-locus datasets based on ITS, LSU, and SSU showed that KNUF-22-14A and KNUF-22-15A formed a distinct cluster from previously identified Tolypocladium species. Thus, these fungal strains isolated from soil in Korea are proposed as a novel species according to their characteristics and are named Tolypocladium globosum sp. nov.

A New Species of Thelonectria and a New Record of Cephalotrichum hinnuleum from Gunwi and Ulleungdo in Korea

( Kallol Das ),( Young-hyun You ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2020 Mycobiology Vol.48 No.5

Three fungal strains belonging to the class Sordariomycetes were isolated from soils collected from Gyeongsangbuk-do in Korea. They were identified as Cephalotrichum hinnuleum (UD CT 1-3-3 and KNU-19GWF1) and Thelonectria chlamydospora sp. nov. (UD ST 1-2-1). T. chlamydospora sp. nov. was morphologically identical to T. truncata, but its specific macroconidial dimensions, lower number of septations, and chlamydospore diameter render it distinct from the strains of the genus Thelonectria. The strains UD CT 1-3-3 and KNU- 19GWF1 were developed flat, velvety to felty, and golden gray to brown-gray after 14 days of incubation at 25 ℃ on PDA. These strains were produced polyblastic conidiogenous cells and conidia were pale brown to brown, smooth, thin-walled, subglobose to ellipsoidal, arranged in chains, and the diameters of 6.7-9.0×3.7-5.1 μm. The strains were also confirmed by using the multi-locus genes using internal transcribed spacer (ITS) regions, partial large subunit (LSU), translation elongation factor 1α (TEF1-α), b-tubulin (TUB2), and actin (ACT) genes. This is the discovery of T. chlamydospora sp. nov. and Cephalotrichum hinnuleum, a new record from Korea.