Genetic Analysis and Molecular Mapping of Gene Associated with Dominant Genic Male Sterility in Rapeseed (Brassica napus L.)

Jun Liu,Deng Feng Hong,Wei Lu,Ping Wu Liu,Qing Biao He,Guang Sheng Yang 한국유전학회 2008 Genes & Genomics Vol.30 No.6

Rs1046AB is a dominant genic male sterility (DGMS) line derived from a spontaneous mutant in Brassica napus. The sterility of this mutant was previously regarded as to be conditioned by the interaction of a dominant male sterility gene Ms and its non-allelic dominant restorer gene Mf (or Rf in previous reports). Recent genetic analyses, however, indicated that Ms and Mf may be allelic. In this research, we confirmed that a multiple allele model should be more appropriate to elucidate the heredity of DGMS line Rs1046AB. Based on this result, the present study emphasized on identifying DNA markers linked to Ms/Mf in an F2 population constructed by crossing Rs1046A with a double haploid (DH) restorer line (19514A). Twenty amplified fragment length polymorphism (AFLP) markers linked to the Ms/Mf locus were identified by combination of bulked segregant analysis (BSA) with AFLP technique. The target locus was detected to be co-segregating with the marker S05T05 and bracketed by two nearest markers, E14M01 and E01M02, with a genetic distance of 0.1 cM and 1.2 cM, respectively. Furthermore, we successfully converted two AFLP markers into sequence characterized amplified region (SCAR) markers. These findings provided direct molecular marker proofs on the inheritance model of this DGMS line, and the high density molecular markers linkage map around the Ms/Mf locus will be more informative for both marker-assisted selection (MAS) of elite male sterile lines and isolation of the Ms/Mf genes by positional cloning in future.

The Future Development of Chinese Enterprises In Northeast Asian Era

( Jun Sheng Liu ) 한국경영사학회 2004 經營史學 Vol.35 No.-

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

Liu, Wei-Jun,Ren, Jian-Guo,Li, Ting,Yu, Guo-Zheng,Zhang, Jin,Li, Chang-Sheng,Liu, Zhi-Su,Liu, Quan-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

Thoracolumbar Injury Classification and Severity Score Is Predictive of Perioperative Adverse Events in Operatively Treated Thoracic and Lumbar Fractures

Liu Gabriel Ka-Po,Tan Jiong Hao,Kong Jun Cheong,Tan Yong Hao Joel,Kumar Nishant,Liang Shen,Shawn Seah Jing Sheng,Ting Chiu Shi,Lim Lau Leok,Dennis Hey Hwee Weng,Kumar Naresh,Thambiah Joseph,Wong Hee-K 대한척추외과학회 2022 Asian Spine Journal Vol.16 No.6

Study Design: A retrospective cohort study of patients with surgically treated thoracolumbar fractures.Purpose: This study aimed to describe the incidence of adverse events (AEs) after surgical stabilization of thoracolumbar spine injuries and to identify predictive factors for the occurrence of AEs. Overview of Literature: Thoracolumbar spine fractures are frequently present in patients with blunt trauma and are associated with significant morbidity. AEs can occur due to the initial spinal injury or secondary to surgical treatment. There is a lack of emphasis in the literature on the AEs that can occur after operative management of thoracolumbar fractures.Methods: We performed a retrospective review of 199 patients with surgically treated thoracolumbar fractures operated between January 2007 and January 2018. The potential risk factors for the development of AEs as well as the development of common complications were evaluated by univariate analysis, and a multivariate logistic regression analysis was performed to identify independent risk factors predictive of the above.Results: The overall rate of AEs was 46.7%; 83 patients (41.7%) had nonsurgical AEs, whereas 24 (12.1%) had surgical adverse events. The most common AEs were urinary tract infections in 43 patients (21.6%), and hospital-acquired pneumonia in 21 patients (10.6%). On multivariate logistic regression, a Thoracolumbar Injury Classification and Severity (TLICS) score of 8–10 (odds ratio [OR], 6.39; 95% confidence interval [CI], 2.33–17.51), the presence of polytrauma (OR, 2.64; 95% CI, 1.17–5.99), and undergoing open surgery (OR, 2.31; 95% CI, 1.09–4.88) were significant risk factors for AEs. The absence of neurological deficit was associated with a lower rate of AEs (OR, 0.47; 95% CI, 0.31–0.70).Conclusions: This study suggests the presence of polytrauma, preoperative American Spinal Injury Association score, and TLICS score are predictive of AEs in patients with surgically treated thoracolumbar fractures. The results might also suggest a role for minimally invasive surgical methods in reducing AEs in these patients.

Up-regulating of RASD1 and Apoptosis of DU-145 Human Prostate Cancer Cells Induced by Formononetin in Vitro

Liu, Xiao-Jia,Li, Yun-Qian,Chen, Qiu-Yue,Xiao, Sheng-Jun,Zeng, Si-En Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Prostate cancer is one of the most prevalent malignant cancers in men. The isoflavone formononetin is a main active component of red clover plants. In the present study, we assessed the effect of formononetin on human prostate cancer DU-145 cells in vitro, and elucidated posssible mechanisms. DU-145 cells were treated with different concentrations of formononetin and cell proliferation was assessed by MTT assay, cell apoptosis by Hoechst 33258 and flow cytometry, and protein levels of RASD1, Bcl-2 and Bax by Western blotting. The results showed that formononetin inhibited the proliferation of DU-145 cells in a dose-dependent manner. DU-145 cells treated with different concentrations of formononetin displayed obvious morphological changes of apoptosis under fluorescence microscopy. In addition, formononetin increased the proportion of early apoptotic DU-145 cells, down-regulated the protein levels of Bcl-2 and up-regulated those of RASD1 and Bax. The level of RASD1 reached its maximum at 48h post-treatment, and rapidly decreased thereafter. Together, we present evidence that formononetin triggered cell apoptosis through the mitochondrial apoptotic pathway by up-regulating RASD1.

Study on the response surface optimization of online upgrading of bio-oil with MCM-41 and catalyst durability analysis

Liu Sha,Cai Yi-xi,Fan Yong-sheng,Li Xiao-hua,Wang Jia-jun 대한환경공학회 2017 Environmental Engineering Research Vol.22 No.1

Direct catalysis of vapors from vacuum pyrolysis of biomass was performed on MCM-41 to investigate the effects of operating parameters including catalyzing temperature, catalyzing bed height and system pressure on the organic yields. Optimization of organic phase yield was further conducted by employing response surface methodology. The statistical analysis showed that operating parameters have significant effects on the organic phase yield. The organic phase yield first increases and then decreases as catalyzing temperature and catalyzing bed height increase, and decreases as system pressure increases. The optimal conditions for the maximum organic phase yield were obtained at catalyzing temperature of 502.7℃, catalyzing bed height of 2.74 cm and system pressure of 6.83 kPa, the organic phase yield amounts to 15.84% which is quite close to the predicted value 16.19%. The H/C, O/C molar ratios (dry basis), density, pH value, kinematic viscosity and high heat value of the organic phase obtained at optimal conditions were 1.287, 0.174, 0.98 g/cm3, 5.12, 5.87 mm2/s and 33.08 MJ/kg, respectively. Organic product compositions were examined using gas chromatography/mass spectrometry and the analysis showed that the content of oxygenated aromatics in organic phase had decreased and hydrocarbons had increased, and the hydrocarbons in organic phase were mainly aliphatic hydrocarbons. Besides, thermo-gravimetric analysis of the MCM-41 zeolite was conducted within air atmosphere and the results showed that when the catalyst continuously works over 100 min, the index of physicochemical properties of bio-oil decreases gradually from 1.15 to 0.45, suggesting that the refined bio-oil significantly deteriorates. Meanwhile, the coke deposition of catalyst increases from 4.97% to 14.81%, which suggests that the catalytic activity significantly decreases till the catalyst completely looses its activity.

Exogenous p53 Upregulated Modulator of Apoptosis (PUMA) Decreases Growth of Lung Cancer A549 Cells

Liu, Chun-Ju,Zhang, Xia-Li,Luo, Da-Ya,Zhu, Wei-Feng,Wan, Hui-Fang,Yang, Jun-Ping,Yang, Xiao-Jun,Wan, Fu-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Purpose: To investigate the influence of exogenous p53 upregulated modulator of apoptosis (PUMA) expression on cell proliferation and apoptosis in human non-small cell lung cancer A549 cells and transplanted tumor cell growth in nude mice. Materials and Methods: A549 cells were divided into the following groups: control, non-carrier (NC), PUMA (transfected with pCEP4-(HA) 2-PUMA plasmid), DDP ($10{\mu}g/mL$ cisplatin treatment) and PUMA+DDP (transfected with pCEP4-(HA)2-PUMA plasmid and $10{\mu}g/mL$ cisplatin treatment). The MTT method was used to detect the cell survival rate. Cell apoptosis rates were measured by flow cytometry, and PUMA, Bax and Bcl-2 protein expression levels were measured by Western blotting. Results: Compared to the control group, the PUMA, DDP and PUMA+DDP groups all had significantly decreased A549 cell proliferation (p<0.01), with the largest reduction in the PUMA+DDP group. Conversely, the apoptosis rates of the three groups were significantly increased (P<0.01), and the PUMA and DDP treatments were synergistic. Moreover, Bax protein levels significantly increased (p<0.01), while Bcl-2 protein levels significantly decreased (p<0.01). Finally, both the volume and the weights of transplanted tumors were significantly reduced (p<0.01), and the inhibition ratio of the PUMA+DDP group was significantly higher than in the single DDP or PUMA groups. Conclusions: Exogenous PUMA effectively inhibited lung cancer A549 cell proliferation and transplanted tumor growth by increasing Bax protein levels and reducing Bcl-2 protein levels.

Similarity Distance Noise Reduction of Entropy Based on Lifting KNN Classification Performance

Liu Jin-sheng,Guoxi Sun,Qinghua Zhang,He jun 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.2

To overcome the drawback of KNN algorithms based on distance measure which did not measure the contributions for each feature accurately. In this paper, a K-Nearest Neighbor (KNN) de-noise method based on likelihood distance entropy is proposed. The relations of feature parameters are used to measure their contributions for de-noise energy, then according to the contributions for each feature leading de-noise of the feature parameters. In order to compare the performance of these relative methods, the Letter corpora and Pima Indians Diabetes data-base are employ to carry out the experiments, the experiment results show that comparing with the other de-noise methods mentioned in this paper, this proposed method have a better ability for de-noise.

Hybridizing gaining–sharing knowledge and differential evolution for large-scale power system economic dispatch problems

Liu Qinghua,Xiong Guojiang,Fu Xiaofan,Mohamed Ali Wagdy,Zhang Jing,Al-Betar Mohammed Azmi,Chen Hao,Chen Jun,Xu Sheng 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.2

Economic dispatch (ED) of thermal power units is significant for optimal generation operation efficiency of power systems. It is a typical nonconvex and nonlinear optimization problem with many local extrema when considering the valve-point effects, especially for large-scale systems. Considering that differential evolution (DE) is efficient in locating global optimal region, while gain-sharing knowledge-based algorithm (GSK) is effective in refining local solutions, this study presents a new hybrid method, namely GSK-DE, to integrate the advantages of both algorithms for solving large-scale ED problems. We design a dual-population evolution framework in which the population is randomly divided into two equal subpopulations in each iteration. One subpopulation performs GSK, while the other executes DE. Then, the updated individuals of these two subpopulations are combined to generate a new population. In such a manner, the exploration and the exploitation are harmonized well to improve the searching efficiency. The proposed GSK-DE is applied to six ED cases, including 15, 38, 40, 110, 120, and 330 units. Simulation results demonstrate that GSK-DE gives full play to the superiorities of GSK and DE effectively. It possesses a quicker global convergence rate to obtain higher quality dispatch schemes with greater robustness. Moreover, the effect of population size is also examined.

Correlation between population size of pear Psylla (Cacopsylla chinensis) and leaf structure features in different pear cultivars

Sheng-jie Xu,Lun Wu,Qi Zhi Liu,Jun Liu,He Wang,Jie-wen Wang,Ting Zhang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2

The feeding preference of pear psylla (Cacopsylla chinensis) varies among pear cultivars. To clarify whether leaf structural factors are related to C. chinensis resistance, eleven pear cultivars of three pear species and their hybrids in China were studied. The population size of C. chinensis in plots of different cultivars was investigated, and leaf paraffin sections of each pear cultivar were observed. Correlation analysis results showed that the thickness of palisade tissue (PT) and the thickness ratio (TR) of PT to leaf (L) were the main factors influencing resistance to C. chinensis. The leaves of cultivar Wujiuxiang and those species from France and the USA exhibited the thickest PT and the highest TR; these three cultivars demonstrated much higher resistance to C. chinensis than did the other cultivars. The cultivar Gold Nijisseiki, with a large C. chinensis population size, showed low PT and TR. The findings of this study may provide valuable information to pear growers, experts and scientists regarding plant protection, cultivation and breeding when selecting suitable varieties to culture from the perspective of pest control.