Chemoenzymatic Synthesis of GDP-L-Fucose Derivatives as Potent and Selective -1,3-Fucosyltransferase Inhibitors

Wei-Che Hsieh,Yu-Nong Lin,Ting-Chien Lin,Chun-Hung Hans Lin 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Fucosyltransferases (FucTs) usually catalyze the final step of glycosylation and are critical to many biological processes. High levels of specific FucT activities are often associated with various cancers. Here we report the development of a chemoenzymatic method for synthesizing a library of GDP-L-fucose derivatives, followed by in situ screening for inhibitory activity against bacterial and human -1,3-FucTs (4). Several compounds incorporating appropriate hydrophobic moieties were identified from the initial screening. These were individually synthesized, purified and characterized in detail for their inhibition kinetics. Compound 5 had a Ki of 29 nM for human FucT-VI, and is 269 and 11 times more selective than for Helicobacter pylori FucT (Ki = 7.8 □ M) and for human FucT-V (Ki = 0.31 □ M).

Tellurium-enhanced nonresonant third-order optical nonlinearity in a germanosilicate optical fiber.

Lin, Aoxiang,Liu, Xueming,Watekar, Pramod R,Zhao, Wei,Peng, Bo,Lu, Min,Wei, Wei,Sun, Chuandong,Wang, Yishan,Han, Won-Taek,Toulouse, Jean Optical Society of America 2009 Applied Optics Vol.48 No.26

<P>A tellurium-doped germanosilicate optical fiber was developed by modified chemical vapor deposition and solution doping techniques. Using the continuous-wave self-phase modulation method, the nonresonant nonlinear refractive index, n2, was measured to be 5.52 x 10(-20) m2/W, which is 2 to 3 times that of the undoped germanosilicate glass fiber. Polyhedron structures TeO3 and TeO4 with GeO4 and SiO4 are believed responsible for lower phonon energy, more nonbridging oxygens, and a larger hyperpolarization, leading to the observed higher nonresonant optical nonlinearity.</P>

Facile Preparation of Holey Anderson-type Polyoxometalate/Polyaniline/Graphene Nanocomposites for Supercapacitors

Jingjing Lin,Song Yan,Xiaojie Zhang,Yueran Liu,Jun Lian,Hualin Lin,Wei Wei,Deli Lu,Sheng Han 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.4

Holey Fe-Anderson-type polyoxometalate/polyaniline/graphene (PPG) hybrid materials were first prepared by anchoring Anderson-type polyoxometalates [FeMo6O24H6]3- (FeMo6) onto graphene modified with polyaniline via a facile hydrothermal treatment. The as-prepared materials exhibited an excellent electrochemical performance with a high specific capacitance of 1366 F g -1 at 1 A g -1 and outstanding cycling stability (97.6% capacitance retention after 5000 cycle times). The uptake of polyaniline/FeMo6 nanoparticles on graphene not only provided the pseudocapacitance but also weakened the aggregation between the graphene layers, resulting in a higher surface area compared with pure graphene. In addition, the AC//PPG-15 asymmetric supercapacitor device showed a high energy density of 24.65 W h kg -1 at a low power density of 326.25 W kg -1 and good cycling stability (94.82% capacitance retention after 5000 cycles). Hence, the as-prepared PPG hybrid materials in this work possess tremendous potential as electrodes for high-performance supercapacitors.

Nuclear Theranostics in Taiwan

Ko-Han Lin,Yi-Wei Chen,Rheun-Chuan Lee,Ling-Wei Wang,Fong-In Chou,Chi-Wei Chang,Sang-Hue Yen,Wen-Sheng Huang 대한핵의학회 2019 핵의학 분자영상 Vol.53 No.2

Boron neutron capture therapy and Y-90 radioembolization are emerging therapeutic methods for uncontrolled brain cancers and hepatic cancers, respectively. These advanced radiation therapies are heavily relied on theranostic nuclear medicine imaging before the therapy for the eligibility of patients and the prescribed-dose simulation, as well as the post-therapy scanning for assessing the treatment efficacy. In Taiwan, the Taipei Veterans General Hospital is the only institute performing the BNCT and also the leading institute performing Y-90 radioembolization. In this article, we present our single institute experiences and associated theranostic nuclear medicine approaches for these therapies.

Systemic Inflammatory Biomarkers, Especially Fibrinogen to Albumin Ratio, Predict Prognosis in Patients with Pancreatic Cancer

Lin Fang,Fei-Hu Yan,Chao Liu,Jing Chen,Dan Wang,Chun-Hui Zhang,Chang-Jie Lou,Jie Lian,Yang Yao,Bo-Jun Wang,Rui-Yang Li,Shu-Ling Han,Yi-Bing Bai,Jia-Ni Yang,Zhi-Wei Li,Yan-Qiao Zhang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1

Purpose Systemic inflammatory response is a critical factor that promotes the initiation and metastasis of malignancies including pancreatic cancer (PC). This study was designed to determine and compare the prognostic value of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), and fibrinogen-to-albumin ratio (FAR) in resectable PC and locally advanced or metastatic PC. Materials and Methods Three hundred fifty-three patients with resectable PC and 807 patients with locally advan-ced or metastatic PC were recruited in this study. These patients were classified into a training set (n=758) and a validation set (n=402). Kaplan-Meier survival plots and Cox proportional hazards regression models were used to analyze prognosis. Results Overall survival (OS) was significantly better for patients with resectable PC with low preoperative PLR (p=0.048) and MLR (p=0.027). Low FAR, MLR, NLR (p < 0.001), and PLR (p=0.003) were significantly associated with decreased risk of death for locally advanced or metastatic PC patients. FAR (hazard ratio [HR], 1.522; 95% confidential interval [CI], 1.261 to 1.837; p < 0.001) and MLR (HR, 1.248; 95% CI, 1.017 to 1.532; p=0.034) were independent prognostic factors for locally advanced or metastatic PC. Conclusion The prognostic roles of FAR, MLR, NLR, and PLR in resectable PC and locally advanced or metastatic PC were different. FAR showed the most prognostic power in locally advanced or metastatic PC. Low FAR was positively correlated with OS in locally advanced or metastatic PC, which could be used to predict the prognosis.

Intense green upconversion emission in Tb^3+/Yb^3+ codoped alumino-germano-silicate optical fibers

Lin, Aoxiang,Liu, Xueming,Watekar, Pramod R.,Guo, Haitao,Peng, Bo,Wei,Lu, Min,Han, Won-Taek,Toulouse, Jean The Optical Society 2010 Applied optics Vol.49 No.9

<P>We observed intense green upconversion emission from Tb(3+) centered at 546 nm due to transition D(4)5-->F(5)7 in Tb(3+)/Yb(3+) codoped alumino-germano-silicate optical fibers by direct excitation of Yb(3+) ions with a laser diode at 976 nm. A two-photon cooperative energy transfer upconversion among a pair of Yb(3+) donor ions and a Tb(3+) acceptor ion are responsible for the observed green emission. Appropriately increasing the concentration of Tb(3+) relative to Yb(3+) was found to facilitate the energy transfer upconversion process from Yb(3+) to Tb(3+), while an excess of Tb(3+) ions inversely introduced more of the concentration quenching effect and phonon-assistant backward energy transfer from Tb(3+) to Yb(3+) and, therefore, damaged the observed green emission. The intensity of the observed green emission was found optimum for a concentration ratio of Tb(3+) to Yb(3+) of 2:1 in alumino-germano-silicate optical glass fibers.</P>

Controlled Layer-by-Layer Etching of MoS₂

Lin, TaiZhe,Kang, BaoTao,Jeon, MinHwan,Huffman, Craig,Jeon, JeaHoo,Lee, SungJoo,Han, Wei,Lee, JinYong,Lee, SeHan,Yeom, GeunYoung,Kim, KyongNam American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.29

<P>Two-dimensional (2D) metal dichalcogenides like molybdenum disulfide (MoS<SUB>2</SUB>) may provide a pathway to high-mobility channel materials that are needed for beyond-complementary metal-oxide-semiconductor (CMOS) devices. Controlling the thickness of these materials at the atomic level will be a key factor in the future development of MoS<SUB>2</SUB> devices. In this study, we propose a layer-by-layer removal of MoS<SUB>2</SUB> using the atomic layer etching (ALET) that is composed of the cyclic processing of chlorine (Cl)-radical adsorption and argon (Ar)<SUP>+</SUP> ion-beam desorption. MoS<SUB>2</SUB> etching was not observed with only the Cl-radical adsorption or low-energy (<20 eV) Ar<SUP>+</SUP> ion-beam desorption steps; however, the use of sequential etching that is composed of the Cl-radical adsorption step and a subsequent Ar<SUP>+</SUP> ion-beam desorption step resulted in the complete etching of one monolayer of MoS<SUB>2</SUB>. Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM) indicated the removal of one monolayer of MoS<SUB>2</SUB> with each ALET cycle; therefore, the number of MoS<SUB>2</SUB> layers could be precisely controlled by using this cyclical etch method. In addition, no noticeable damage or etch residue was observed on the exposed MoS<SUB>2</SUB>.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-29/acsami.5b03491/production/images/medium/am-2015-03491k_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b03491'>ACS Electronic Supporting Info</A></P>

BMB Reports : Characterization of a novel posttranslational modification in polypyrimidine tract-binding proteins by SUMO1

( Wei Han ),( Lin Wang ),( Bin Yin ),( Xiao Zhong Peng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.4

Polypyrimidine tract-binding protein 1 (PTBP1) and its brainspecific homologue, PTBP2, are associated with pre-mRNAs and influence pre-mRNA processing, as well as mRNA metabolism and transport. They play important roles in neural differentiation and glioma development. In our study, we detected the expression of the two proteins in glioma cells and predicted that they may be sumoylated using SUMOplot analyses. We confirmed that PTBP1 and PTBP2 can be modified by SUMO1 with co-immunoprecipitation experiments using 293ET cells transiently co-expressing SUMO1 and either PTBP1 or PTBP2. We also found that SUMO1 modification of PTBP2 was enhanced by Ubc9 (E2). The mutation of the sumoylation site (Lys137) of PTBP2 markedly inhibited its modification by SUMO1. Interestingly, in T98G glioma cells, the level of sumoylated PTBP2 was reduced compared to that of normal brain cells. Overall, this study shows that PTBP2 is posttranslationally modified by SUMO1. [BMB Reports 2014; 47(4): 233-238]

Recent advances in the food application of electrospun nanofibers

Wei-Hua Han,Xiao Li,Gui-Feng Yu,Bin-Chang Wang,Lin-Peng Huang,Jingkai Wang,Yun-Ze Long 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

Electrospinning (e-spinning) has excellent advantages in producing functional and versatile nanofibersand electrospun (e-spun) nanofibers have a variety of potential applications, including in food industry. This paper reviews the recent progress of e-spinning and food-related e-spun nanofibers, covering ediblenanofibers (natural materials and food ingredients), active food packaging (antibacterial packaging,antioxidant packaging, high temperature and humidity resistant packaging), food filtration membranes,and food sensors. Additionally, we discussed the new trend of combining nanomaterials with food andthe challenges of applying e-spinning in food science. The results indicated that e-spinning is a promisingand efficient alternative for the production of daily and functional food.

Electroencephalographic spectrogram–guided total intravenous anesthesia using dexmedetomidine and propofol prevents unnecessary anesthetic dosing during craniotomy: a propensity score–matched analysis

Lin Feng-Sheng,Shih Po-Yuan,Sung Chao-Hsien,Chou Wei-Han,Wu Chun-Yu 대한마취통증의학회 2024 Korean Journal of Anesthesiology Vol.77 No.1

Background: The bispectral index (BIS) may be unreliable to gauge anesthetic depth when dexmedetomidine is administered. By comparison, the electroencephalogram (EEG) spectrogram enables the visualization of the brain response during anesthesia and may prevent unnecessary anesthetic consumption. Methods: This retrospective study included 140 adult patients undergoing elective craniotomy who received total intravenous anesthesia using a combination of propofol and dexmedetomidine infusions. Patients were equally matched to the spectrogram group (maintaining the robust EEG alpha power during surgery) or the index group (maintaining the BIS score between 40 and 60 during surgery) based on the propensity score of age and surgical type. The primary outcome was the propofol dose. Secondary outcome was the postoperative neurological profile.Results: Patients in the spectrogram group received significantly less propofol (1585 ± 581 vs. 2314 ± 810 mg, P < 0.001). Fewer patients in the spectrogram group exhibited delayed emergence (1.4% vs. 11.4%, P = 0.033). The postoperative delirium profile was similar between the groups (profile P = 0.227). Patients in the spectrogram group exhibited better in-hospital Barthel’s index scores changes (admission state: 83.6 ± 27.6 vs. 91.6 ± 17.1; discharge state: 86.4 ± 24.3 vs. 85.1 ± 21.5; group–time interaction P = 0.008). However, the incidence of postoperative neurological complications was similar between the groups.Conclusions: EEG spectrogram–guided anesthesia prevents unnecessary anesthetic consumption during elective craniotomy. This may also prevent delayed emergence and improve postoperative Barthel index scores.