Transcriptome-based deep learning analysis identifies drug candidates targeting protein synthesis and autophagy for the treatment of muscle wasting disorder

Lee Min Hak,Lee Bada,Park Se Eun,Yang Ga Eul,Cheon Seungwoo,Lee Dae Hoon,Kang Sukyeong,Sun Ye Ji,Kim Yongjin,Jung Dong-sub,Kim Wonwoo,Kang Jihoon,Kim Yi Rang,Choi Jin Woo 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

Sarcopenia, the progressive decline in skeletal muscle mass and function, is observed in various conditions, including cancer and aging. The complex molecular biology of sarcopenia has posed challenges for the development of FDA-approved medications, which have mainly focused on dietary supplementation. Targeting a single gene may not be sufficient to address the broad range of processes involved in muscle loss. This study analyzed the gene expression signatures associated with cancer formation and 5-FU chemotherapy-induced muscle wasting. Our findings suggest that dimenhydrinate, a combination of 8-chlorotheophylline and diphenhydramine, is a potential therapeutic for sarcopenia. In vitro experiments demonstrated that dimenhydrinate promotes muscle progenitor cell proliferation through the phosphorylation of Nrf2 by 8-chlorotheophylline and promotes myotube formation through diphenhydramine-induced autophagy. Furthermore, in various in vivo sarcopenia models, dimenhydrinate induced rapid muscle tissue regeneration. It improved muscle regeneration in animals with Duchenne muscular dystrophy (DMD) and facilitated muscle and fat recovery in animals with chemotherapy-induced sarcopenia. As an FDA-approved drug, dimenhydrinate could be applied for sarcopenia treatment after a relatively short development period, providing hope for individuals suffering from this debilitating condition.

Leiomyomatosis peritonealis disseminata: A case report

( Ga Eul Lee ),( Jong Hyun Kim ),( Chul Hee Rheu ),( Byung Chan Oh ),( Dong Hyu Cho ) 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-

The effect of combined hemostatic techniques during cesarean section in placenta previa; preliminary study

( Ga Eul Lee ),( Young Ju Jeong ),( Sung Nam Cho ),( Jeong Heon Lee ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: Our objective was to evaluate the effect of combined use of transient bilateral ovarian artery clamping, uterine artery ligation, and Bakri balloon tamponade during cesarean section in women with placenta previa. 방법: We performed a retrospective cohort study of all cesarean deliveries performed due to placenta previa from April 2013 until June 2016 at Chonbuk National University Hospital. Charts were reviewed to determine the amount of RBC transfusion, degree of blood loss and incidence of postpartum hysterectomy and uterine artery embolization (UAE) between conventional method and combined technique to control hemorrhage during cesarean section for placenta previa. Patients with unsortable technique were excluded. The outcome was analyzed using independent t-test. 결과: There was a total of 118 cesarean deliveries for placenta previa during the study period, with two groups of 100 patients using conventional hemostatic method and 17 patients using combined hemostatic technique. There were not different between two groups in the mean amount of PRC transfusion during operation (0.88 ± 2.12 unit vs. 0.24 ± 0.66 units, respectively, p=0.22) and the index of blood loss (0.22 ± 0.13 vs. 0.23 ± 0.14, respectively, p=0.78). The incidence of postpartum hysterectomies (0.05 ± 0.22 vs. 0 ± 0, respectively, p=0.03) and postpartum care (0.13 ± 0.46 vs. 0 ± 0, respectively, p=0.01) for continuous bleeding after cesarean section were significantly low in combined technique group. 결론: Our findings show that utilization of the combined technique (transient bilateral ovarian artery clamping, uterine artery ligation, and Bakri balloon tamponade) during cesarean section for placenta previa is associated with a decreased rate of postpartum hysterectomy and additional hemostatic procedure after cesarean section.

디자인 분야가 상·장례(喪·葬禮)문화에 미치는 역할에 대한 연구- 변화 하는 상장례문화를 중심으로

이가을(Ga eul Lee),김선철(Sunchul Kim) 경희대학교 산학협력단 예술디자인연구원 2016 예술· 디자인학연구 Vol.19 No.1

The people today are questioning the existing funeral industries, and expecting a new kind of funeral-related industries. According to the research done with many unspecified people, the future funerals are expected to be different from the traditional 3-day funerals, creating unique and ingenious funerary culture and service design. Currently, new types of funerary culture design products are already being launched, and in the future the field of design will give a great influence on the funerary culture.

일선 도장 검도사범의 지도체험에 관한 연구

이가을(Ga-Eul Lee),김영학(Young-Hak Kim) 대한검도학회 2007 대한검도학회지 Vol.23 No.1

본 연구는 일선 검도장 사법의 검도지도체험을 질적 연구 방법을 사용하여 탐색하였다. 이를 위하여 집단 심층 면담(focus group interview) 방법을 사용하였으며, 2개의 도장 2명의 사범을 대상으로, 집단 심층 면담(focus group interview), 비참여 관찰(nonparticipant observatiom), 관련 문서 수집(document analysis) 등의 방법을 이용하였으며, 귀납적 내용분석(inductive content analysis) 방법을 사용하여 분석하였다. 이에 대한 결론은, 첫째, 검도선수에서 검도사법으로의 변화에 있어서 연구대상자는 검도실력, 노력, 공부 기록, 전문지식 습득의 필요성을 강조하는 것으로 나타났다. 둘째, 검도 사범들은 학교운동부와의 차별화에 있어 흥미와 기초 및 기본교육, 그리고 예법을 중시하고 있는 것으로 나타났다. 셋째 일선 검도사법들은 지도철학으로 예의, 겸손이라는 주제어로 나타났다. 넷째, 검도사범들이 지도자로서의 길을 걸으면서, 학교운동부에서의 생활과는 달리 지도하는 언어에 대한 정리, 프로그램의 개발, 지도자로서의 행동규범 등 변화가 이루어지고 있음을 알 수 있었다. 다섯째, 사회교육의 일환으로 사회교육자 입장에 서 있는 점에서 수련생들에게 건강과 용기를 줄 수 있다는 것에 대해 물질적인 보상보다 크고 검도에 대한 열정에 보람과 성취감을 찾고 있는 것으로 나타났다. 여섯째, 일선 도장의 검도사범들은 직업으로서 급여가 낮고, 안정성이 부족한 직장이라는 점, 그리고 사회적인 인지도가 높지 않는 등 열악한 환경에 있는 것으로 나타났다. This research was conducted for the purpose of studying the quality of Kumdo master experience by Kumdo School master. For the research, the focus group interview was done on 2 masters from 2 Kumdo school. As for the research, the focus group interview, non participant observation and document analysis were conducted and analyzed through inductive content analysis. The conclusion are as follows; First of all, in order for a Kumdo student to become a master, the research subject needs to have a Kumdo skill, show effort, study, and record and acquire professional knowledge. Secondly, the Kumdo master needs to bo differentiated from school sports unit by putting importance on the interest, basic skill, basic education and manner. Thirdly, the Kumdo masters emphasized the terms courtesy and humbleness. Fourth, the Kumdo masters realized that unlike school sports department, the coaching experience brought change in speaking, on developing program and in the behavior as the leader. Fifth, as the member of social education, the coaches experienced passion, worthiness and sense of accomplishment about Kumdo as they encouraged and improved the health of the students, which is far greater than a material reward. Sixth, it has been found that the Kumdo-school masters are in fragile situation because of their low pay, unstableness of the job and low acknowledgement by the society.

아시아 국가들의 주관적 건강상태에 미치는 영향요인 분석

이가을(Ga-eul Lee),변병설(Byungseol Byun) 한국자료분석학회 2023 Journal of the Korean Data Analysis Society Vol.25 No.5

본 연구는 아시아지역의 건강지수와 실제 건강 수준 간 편차를 줄이는 방법으로 주관적 건강상태(Self-rated health)를 활용하였다. 유엔 개발 프로그램(UNDP)의 Human Development Index(HDI) 점수를 이용하여 건강 수준이 상대적으로 높은 나라(A그룹), 중간수준인 나라(B그룹), 상대적으로 낮은 나라(C그룹)로 그룹화하고, 건강 수준이 비슷한 그룹별로 주관적 건강상태에 영향을 미치는 요인을 로짓모형을 통해 분석하였다. 독립변수로는 주관적 건강상태에 영향을 미치는 심리적, 정치적, 경제적, 사회적, 환경적 요인을 세계 가치관 조사(World Values Survey) 자료를 이용하여 도출하였으며, 종속변수는 주관적 건강상태를 분석하였다. 공간적 범위는 아시아 국가들로 데이터의 범위 내에서 동북아시아인 중국, 일본, 한국 3개국과 동남아시아인 방글라데시, 인도네시아, 말레이시아, 미얀마, 파키스탄, 필리핀, 태국, 베트남 8개국을 포함하였다. 시간적 범위는 2017년에서 2019년으로 WVS 데이터 Wave 7에 해당한다. 로지스틱 회귀분석 결과, 세 그룹에 공통으로 영향을 미치는 변수는 심리적 요인 중 삶의 만족도와 행복감, 경제적 요인 중 재정상태 만족도로 나타났으며, 사회적 요인 중 이웃신뢰, 환경적 요인 중 의료여건으로 나타났다. 건강수준이 상대적으로 높은 A그룹은 전반적으로 영향을 미치고 있는 심리적 요인과 경제적 요인변수들을 유지할 필요가 있으며, B, C그룹에 비해서 상대적으로 낮은 정도를 나타내고 있는 정부신뢰, 이웃신뢰, 단체활동, 경쟁인식, 생활안전 변수들의 만족도 향상을 위한 정책 마련이 필요함을 시사하고 있다. 건강지수가 중간수준인 B그룹은 A, C그룹에 비해 상대적으로 높은 정도를 나타내고 있는 정치적, 사회적 요인을 유지하는 방향으로 건강정책을 다뤄야 하며, 건강지수가 상대적으로 낮은 C그룹은 전반적으로 영향을 미치고 있는 심리적 요인과 환경적 요인을 중점으로 건강정책을 개선해나갈 필요가 있을 것이다. This study utilized self-rated health as a method of reducing the deviation between health index and actual health level in Asia. Using the Human Development Index (HDI) score of the United Nations Development Program (UNDP), countries were grouped into countries with relatively high health (Group A), middle-level countries (Group B), and countries with relatively low levels of health (Group C), Factors affecting subjective health status for each group with similar health levels were analyzed using the logit model. As an independent variable, psychological, political, economic, social, and environmental factors affecting subjective health were derived using World Values Survey (WVS) data, and the dependent variable was subjective health status. Spatial coverage includes Asian countries, including three Northeast Asian countries, China, Japan, and South Korea, and Southeast Asian countries, Bangladesh, Indonesia, Malaysia, Myanmar, Pakistan, Philippines, Thailand, and Vietnam within the scope of the data. The temporal range corresponds to WVS data Wave 7 from 2017 to 2019. As a result of logistic regression analysis, the variables common to the three groups were life satisfaction and happiness among psychological factors, financial condition satisfaction among economic factors, trust in neighbors among social factors, and medical condition among environmental factors. Group A, which has a relatively high level of health, needs to maintain the psychological and economic factors that have an overall influence, and trust in government, trust in neighbors, group activities, and competition, which are relatively low compared to groups B and C. This suggests that it is necessary to prepare policies to improve awareness and satisfaction with life safety variables. Group B, which has a moderate health index, has to deal with health policies in the direction of maintaining the relatively high level of political and social factors compared to groups A and C. Group C, which has a relatively low health index, has an overall impact. There is a need to improve health policies focusing on psychological and environmental factors that are affecting people's health.

지자체 역량이 재난 회복탄력성에 미치는 영향 분석: 17개 광역자치단체를 중심으로

이가을(Ga-eul Lee),변병설(Byung-seol Byun) 한국환경정책학회 2020 環境政策 Vol.28 No.4

평판 스크린을 이용하는 롤투롤 연속 스크린 인쇄 시스템의 개발

김가을(Ga Eul Kim),전용호(Yong Ho Jeon),이문구(Moon Gu Lee),홍민성(Min Sung Hong),이택민(Taik Min Lee),권신(Sin Kwon) 한국생산제조학회 2016 한국생산제조학회지 Vol.25 No.3

In this research, a continuous roll-to-roll screen printing system was developed using a flat screen. It has a newly devised sliding mechanism of screen printing module, which can be controlled accurately in sync with a moving web, driven by a roll-to-roll tension control and web-guiding system. In addition, the real-time precision alignment module that consists of a vision camera and an X-Y-θ alignment stage was implemented. With this developed system, the feasibility of continuous printing with minimum pattern width below 60 μm was verified, and an overlay of ±60 μm between the laser-patterned reference mark and the printed mark on a 300-mm-wide film was achieved.

롤투롤 나노 임프린트 시스템 균일 가압 모듈 개발

김가을(Ga Eul Kim),김현태(Hyuntae Kim),이승현(Seung-Hyun Lee),강유성(Yousung Kang),전용호(Yongho Jeon),이문구(Moon Gu Lee),권신(Sin Kwon) 한국생산제조학회 2021 한국생산제조학회지 Vol.30 No.4

It is difficult to use roll-to-roll (R2R) substrate transfer and pressing process to secure the productivity of the nanoimprint lithography (NIL) process based on the contact pressing mechanism R2R NIL patterning with a precise level of uniform pressure in the imprinting area. In conventional pressing, the precision of the imprint process is limited by bending deformation of the pressing roller, processing errors of roundness and straightness, and roller alignment errors. Thus, a new type of force point adjustable pressing roller (FPAP) module to minimize bending deformation of the pressing roller and an air cushion pressing roller (ACP) module to improve unevenness caused by roller deformation, roller geometrical errors were developed. The performance of the new uniform pressing module was experimentally verified: pressure uniformity at the imprinting contact surface was ±30% for the conventional pressing module compared to ±7% for the FPAP module. The ACP module improved this value to ±3.5%.

발효수산물로부터 분리된 호염성 미생물의 특성 분석

정가을 ( Ga Eul Jeong ),간밧다리마 ( Dariimaa Ganbat ),염유정 ( Yujeong Yeom ),최보경 ( Bo Gyeong Choi ),이한승 ( Han-seung Lee ),박미화 ( Mi-hwa Park ),신기선 ( Kee-sun Shin ),이용직 ( Yong-jik Lee ),이상재 ( Sang-jae Lee ) 한국산업식품공학회 2021 산업 식품공학 Vol.25 No.2

본 연구는 국내외 해양유래 발효산물 시료로부터 분리한 호염성 미생물들의 다양성 및 특성에 관하여 조사하였다. 호염성 미생물의 순수 분리를 위하여 marine agar 배지를 사용하였으며 25, 37, 50℃에서 호기적으로 배양하였다. 순수 분리 후, 86균주를 분리하였으며 16S rRNA 염기서열 분석 결과를 바탕으로 계통학적 분석을 실시한 결과, 3문, 7과, 9속, 24종으로 구성되어 있는 것을 확인하였다. 특히 Firmicutes문 Bacilli강은 84.9%의 분포를 나타내었으며 4과, 6속, 19종으로 Bacillaceae, Planococcaceae, Staphylococcaceae와 Enterococcaceae로 분포하는 것을 확인하였다. 그리고 분리한 균주들이 amylase, lipase, cellulase, protease 같은 산업적으로 유용한 효소를 생산하는지 확인하기 위하여 효소 활성 평가를 실시하였으며, 55 균주가 최소 한 종류 이상의 효소 활성을 가지고 있는 것을 확인하였다. 또한 옥신 생산능을 가지는 균주도 2 균주가 확인되었으며 이는 본 연구를 통하여 분리한 미생물들의 산업적 활용 가능성을 나타내었다. 그러므로 이번 연구는 국내 유전자원 확보 및 시료의 호염성 미생물의 다양성과 특성에 관한 과학적 지식 확장에 도움이 될 것으로 생각된다. To identify the diversity of halophilic bacteria from fermented seafoods, 86 strains were isolated and a phylogenetic analysis was carried out based on the results of 16S rRNA gene sequencing. The isolated strains were divided into 3 phyla, 7 families, 9 genera, and 24 species. Bacilli class, the main phyletic group, comprised 84.9% with 4 families, 6 genera, and 19 species of Bacillaceae, Planococcaceae, Staphylococcaceae, and Enterococcaceae. The strains were tested for amylolytic, cellulolytic, lipolytic, and proteolytic activity and 55 strains showed at least one enzyme activity. Furthermore, auxin activity was determined in two strains. These results indicate that the isolated strains have the possibility for application in the food and feed industries and of being important genetic resources in Korea.