http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Antioxidant Capacity of Novel Pigments from an Antarctic Bacterium
Daniela N. Correa-Llantén,Maximiliano J. Amenábar,Jenny M. Blamey 한국미생물학회 2012 The journal of microbiology Vol.50 No.3
In Antarctica microorganisms are exposed to several conditions that trigger the generation of reactive oxygen species, such as high UV radiation. Under these conditions they must have an important antioxidant defense system in order to prevent oxidative damage. One of these defenses are pigments which are part of the non-enzymatic antioxidant mechanisms. In this work we focused on the antioxidant capacity of pigments from an Antarctic microorganism belonging to Pedobacter genus. This microorganism produces different types of pigments which belong to the carotenoids group. The antioxidant capacity of a mix of pigments was analyzed by three different methods: 1,1-diphenyl-2-picrylhydrazyl, ROS detection and oxygen electrode. The results obtained from these approaches indicate that the mix of pigments has a strong antioxidant capacity. The oxidative damage induced by UVB exposure to liposomes was also analyzed. Intercalated pigments within the liposomes improved its resistance to lipid peroxidation. Based on the analysis carried out along this research we conclude that the antioxidant properties of the mix of pigments protect this bacterium against oxidative damage. These properties make this mix of pigments a powerful antioxidant mixture with potential biotechnological applications.
Resistance to Hypoosmotic Shock of Liposomes Containing Novel Pigments from an Antarctic Bacterium
Daniela N. Correa-Llanten,Maximiliano J. Amenabar,Jenny M. Blamey 한국미생물·생명공학회 2012 한국미생물·생명공학회지 Vol.40 No.3
Although the antioxidant capacity of carotenoids and their role in regulating membrane fluidity have been well studied, their ability to confer resistance to hypoosmotic shock is poorly understood. In this work, we analyzed the effect of a mixture of carotenoid pigments obtained from an Antarctic microorganism belonging to the genus Pedobacter on liposomal resistance to hypoosmotic conditions. Intercalation of pigments into liposomal structures resulted in an improvement of membrane resistance by decreasing the percentage of calcein released in comparison to that by liposomes without pigments. Due to these properties, such pigments could be useful for biotechnological applications.
Resistance to Hypoosmotic Shock of Liposomes Containing Novel Pigments from an Antarctic Bacterium
Correa-Llanten, Daniela N.,Amenabar, Maximiliano J.,Blamey, Jenny M. The Korean Society for Microbiology and Biotechnol 2012 한국미생물·생명공학회지 Vol.40 No.3
Although the antioxidant capacity of carotenoids and their role in regulating membrane fluidity have been well studied, their ability to confer resistance to hypoosmotic shock is poorly understood. In this work, we analyzed the effect of a mixture of carotenoid pigments obtained from an Antarctic microorganism belonging to the genus Pedobacter on liposomal resistance to hypoosmotic conditions. Intercalation of pigments into liposomal structures resulted in an improvement of membrane resistance by decreasing the percentage of calcein released in comparison to that by liposomes without pigments. Due to these properties, such pigments could be useful for biotechnological applications.
Joseph M. Escandón,Eric Santamaría,Peter A. Prieto,Daniela Duarte-Bateman,Pedro Ciudad,Megan Pencek,Howard N. Langstein,Hung-Chi Chen,Oscar J. Manrique 대한성형외과학회 2022 Archives of Plastic Surgery Vol.49 No.3
Several reconstructive methods have been reported to restore the continuity of the aerodigestive tract following resection of pharyngeal and hypopharyngeal cancers. However, high complication rates have been reported after voice prosthesis insertion. In this setting, the ileocolon free flap (ICFF) offers a tubularized flap for reconstruction of the hypopharynx while providing a natural phonation tube. Herein, we systematically reviewed the current evidence on the use of the ICFF for reconstruction of the aerodigestive tract. A systematic literature search was conducted across PubMed MEDLINE, Web of Science, ScienceDirect, Scopus, and Ovid MEDLINE(R). Data on the technical considerations and surgical and functional outcomes were extracted. Twentyone studies were included. The mean age and follow-up were 54.65 years and 24.72 months, respectively. An isoperistaltic or antiperistaltic standard ICFF, patch flap, or chimeric seromuscular-ICFF can be used depending on the patients’ needs. The seromuscular chimeric flap is useful to augment the closure of the distal anastomotic site. The maximum phonation time, frequency, and sound pressure level (dB) were higher with ileal segments of 7 to 15 cm. The incidence of postoperative leakage ranged from 0 to 13.3%, and the majority was occurring at the coloesophageal junction. The revision rate of the microanastomosis ranged from 0 to 16.6%. The ICFF provides a reliable and versatile alternative for reconstruction of middle-size defects of the aerodigestive tract. Its three-dimensional configuration and functional anatomy encourage early speech and deglutition without a prosthetic valve and minimal donor-site morbidity.
Daniela Sánchez Aldana,Cristobal Noé Aguilar,Juan Carlos Contreras-Esquivel,Marthyna Pessoa Souza,Maria das Graças Carneiro-da-Cunha,Guadalupe Virginia Nevárez-Moorillón 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.5
This work aimed to develop an edible coating based on Mexican lime ( Citrus aurantifolia Swingle) pectic extract and essentialoil on Haden mango ( Mangifera indica L.) to extend its shelf life. Mango cubes were coated by immersion in a lime pecticextract (1% pectin w/v), lime essential oil (0.05% v/v), and glycerol (0.7% v/v) solution for 2, 5, and 10 min. Subsequently,coated and uncoated (control) test samples were stored for 21 days, and physical–chemical and microbiological analyseswere performed every 3 days. The results showed no signifi cant diff erences for total soluble solids, pH, and fi rmness. On thesixth day, bacterial growth was signifi cantly lower in coated mangos than in the control (log 6.08 ± 0.49 and 7.63 ± 0.20 UFCg −1 , respectively). The application of the edible coating extended the shelf life of minimally processed mangos by 3 days,delaying physical and chemical changes as well as bacterial growth.
Cancer-Associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma
Pankova, Daniela,Chen, Yulong,Terajima, Masahiko,Schliekelman, Mark J.,Baird, Brandi N.,Fahrenholtz, Monica,Sun, Li,Gill, Bartley J.,Vadakkan, Tegy J.,Kim, Min P.,Ahn, Young-Ho,Roybal, Jonathon D.,Liu American Association for Cancer Research 2016 Molecular Cancer Research Vol.14 No.3
<P>Intratumoral collagen cross-links heighten stromal stiffness and stimulate tumor cell invasion, but it is unclear how collagen cross-linking is regulated in epithelial tumors. To address this question, we used Kras(LA1) mice, which develop lung adenocarcinomas from somatic activation of a Kras(G12D) allele. The lung tumors in KrasLA1 mice were highly fibrotic and contained cancer-associated fibroblasts (CAF) that produced collagen and generated stiffness in collagen gels. In xenograft tumors generated by injection of wild-type mice with lung adenocarcinoma cells alone or in combination with CAFs, the total concentration of collagen cross-links was the same in tumors generated with or without CAFs, but coinjected tumors had higher hydroxylysine aldehyde-derived collagen cross-links (HLCC) and lower lysine-aldehyde-derived collagen cross-links (LCCs). Therefore, we postulated that an LCC-to-HLCC switch induced by CAFs promotes the migratory and invasive properties of lung adenocarcinoma cells. To test this hypothesis, we created coculture models in which CAFs are positioned interstitially or peripherally in tumor cell aggregates, mimicking distinct spatial orientations of CAFs in human lung cancer. In both contexts, CAFs enhanced the invasive properties of tumor cells in three-dimensional (3D) collagen gels. Tumor cell aggregates that attached to CAF networks on a Matrigel surface dissociated and migrated on the networks. Lysyl hydroxylase 2 (PLOD2/LH2), which drives HLCC formation, was expressed in CAFs, and LH2 depletion abrogated the ability of CAFs to promote tumor cell invasion and migration.</P>
Resistance to Hypoosmotic Shock of Liposomes Containing Novel Pigments from an Antarctic Bacterium
( Correa Llanten ),( Daniela N. ),( Maximiliano J. Amenabar ),( Jenny M. Blamey ) 한국미생물생명공학회(구 한국산업미생물학회) 2012 한국미생물·생명공학회지 Vol.40 No.3
Although the antioxidant capacity of carotenoids and their role in regulating membrane fluidity have been well studied, their ability to confer resistance to hypoosmotic shock is poorly understood. In this work, we analyzed the effect of a mixture of carotenoid pigments obtained from an Antarctic microorganism belonging to the genus Pedobacter on liposomal resistance to hypoosmotic conditions. Intercalation of pigments intoliposomal structures resulted in an improvement of membrane resistance by decreasing the percentage of calcein released in comparison to that by liposomes without pigments. Due to these properties, such pigments could be useful for biotechnological applications.
Soccio, Mario,Trono, Daniela,Laus, Maura N.,Pastore, Donato 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.4
An improved spectrophotometric assay of phospholipase $A_2$ ($PLA_2$) activity based on the coupled $PLA_2$/lipoxygenase (LOX) reactions using 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphatidylcholine ($PC_{LIN}$) as substrate is reported. The $PLA_2$-mediated release of free linoleate is continuously monitored by following the absorbance increase at 234 nm caused by its conversion into the conjugated diene hydroperoxide catalyzed by the coupled soybean LOX-1 reaction. The new protocol includes the use of Tween 20 ($3{\mu}L/{\mu}mol$ phospholipid) as surfactant and of ethanol ($15{\mu}L/mL$ reaction mixture), that ensure clearness of reaction mixture and linear increase of absorbance in the course of reaction. This method was tested on a purified secretory $PLA_2$ from honey bee venom (HBV-$PLA_2$). The enzyme did not discriminate among $PC_{LIN}$, phosphatidylcholine, and phosphatidylethanolamine, but showed the highest rate using 1,2-dilinoleoyl-sn-glycero-3-phosphatidylcholine ($PC_{DILIN}$). Nevertheless, the use of $PC_{DILIN}$ is not recommended, as it may induce an overestimation of enzyme activity, because not only the free linoleate, but also the reaction product 1-linoleoyl-lysophosphatidylcholine, are known to be oxidized by LOX. HBV-$PLA_2$ showed maximal activity at pH 9.0, hyperbolic kinetics ($K_m$, $74.2{\pm}2.9{\mu}M$; $V_{max}$, $827{\pm}7{\mu}mol/min/mg$ protein) and competitive inhibition ($K_i$ about $5{\mu}M$) by palmityl trifluoromethyl ketone, a classical $PLA_2$ inhibitor. Interestingly, the HBV-$PLA_2$/soybean LOX-1 coupled reactions also allow an accurate assay of $PC_{LIN}$ concentration. In the whole, these results demonstrate that this improved $PLA_2$/LOX assay allows a very accurate, simple, and rapid measurement of enzyme activity and substrate concentration.
Mario Soccio,Donato Pastore,Daniela Trono,Maura N. Laus 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.4
An improved spectrophotometric assay of phospholipase A2 (PLA2) activity based on the coupled PLA2/lipoxygenase (LOX) reactions using 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphatidylcholine (PCLIN) as substrate is reported. The PLA2-mediated release of free linoleate is continuously monitored by following the absorbance increase at 234 nm caused by its conversion into the conjugated diene hydroperoxide catalyzed by the coupled soybean LOX-1 reaction. The new protocol includes the use of Tween 20 (3 μL/μmol phospholipid) as surfactant and of ethanol (15 μL/mL reaction mixture), that ensure clearness of reaction mixture and linear increase of absorbance in the course of reaction. This method was tested on a purified secretory PLA2from honey bee venom (HBV-PLA2). The enzyme did not discriminate among PCLIN, phosphatidylcholine, and phosphatidylethanolamine,but showed the highest rate using 1,2-dilinoleoyl-sn-glycero-3-phosphatidylcholine (PCDILIN). Nevertheless,the use of PCDILIN is not recommended, as it may induce an overestimation of enzyme activity, because not only the free linoleate, but also the reaction product 1-linoleoyl-lysophosphatidylcholine,are known to be oxidized by LOX. HBV-PLA2showed maximal activity at pH 9.0, hyperbolic kinetics (Km,74.2±2.9 μM; Vmax, 827±7 μmol/min/mg protein) and competitive inhibition (Ki about 5 μM) by palmityl trifluoromethyl ketone, a classical PLA2 inhibitor. Interestingly, the HBV-PLA2/soybean LOX-1 coupled reactions also allow an accurate assay of PCLIN concentration. In the whole, these results demonstrate that this improved PLA2/LOX assay allows a very accurate, simple, and rapid measurement of enzyme activity and substrate concentration.