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무배란성 불임환자에 있어서 골반 초음파 추적조사에 의한 Gonadotropin 치료
이두룡,김덕만,조치흠 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.3
Ovarian follicular growth was monitored by ultrasound. Ovulatioin induction in 25 cycles of 13 anovulatory infertility patients were performed. These women were treated with clomiphene citrate, human menopausal gonadotropin(hMG) and human chorionic gonadotropin(hCG) for anovulatry infertility. The amount of gonadotropins administered were based exclusively on the results of the ultrasound examiinations. Twelve pregnancies were obtained. with 12 singletons, resulting in accumulative pregnancy rate about 92.3%. Ultrasoound can be helpful in the determination of the best time for induction of ovulation and effectively to control gonadotropin therapy in the majority of cases.
조치흠 ( Chi Heum Cho ),( Hyun Gyo Lee ),( Ji Min Lee ),( So Jin Shin ),( Sang Hoon Kwon ),( Gi Su Lee ),( Chang Ho Song ),( Eun Som Choi ),( Soon Do Cha ) 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-
Objective: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. Methods: Cell viability was measured by MIT (3-(4 ,5-dimelhyllhiazol-2-yl)-2,S-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot. Results: The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed. Conclusion: These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.
조치흠(Chi Heum Cho),차순도(Soon Do Cha),백원기(Won Ki Baek),권건영(Kun Young Kwon),배인수(In Soo Bae) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.5
N/A Objective : Apoptosis or programmed cell death is a normally physiological cell suicide program that is highly conserved among all animals. We previously evaluated overexpression of c-IAP1(Inhibitor of Apoptosis Protein) in ovarian carcinomas compared with normal ovaries. In this study, we demonstrate evidence for the involvement of c-IAP2 in ovarian carcinomas. Methods : Fresh 9 normal ovaries, 5 benign ovarian cysts and 13 ovarian carcinomas were obtained from routine gynecologic surgeries carried out for benign and malignant ovarian tumors. They were examined for the presence of c-IAP2 by RT-PCR(Reverse Transcriptase Polymerase Chain Reaction), Western blot analysis and immunohistochemical stains. Results : Nine of 14 normal and benign ovarian tumors were negative and 11 of 13 ova rian carcinomas were positive for c-IAP2 by RT-PCR. Positive RT-PCR for c-IAP2 was seen in 11/13 of ovarian carcinomas, a significantly higher percentage than in normal and benign ovarian tumors(5/14). All of these tumors showed strong positive for c-IAP2 by western blot and immunohistochemical staining. Whereas negative RT-PCR for c-IAP2 was seen in 9/14 of normal and benign ovarian tumors, a significantly higher percentage than ovarian carcinomas(2/13). Of these 9 negative samples, 6 had positive Western blot and immunohistochemical stains. There was weak concordance of the result. But expression of c-IAP2 in normal ovarian tissue was localized exclusively in the corpus luteum. Therefore, c-IAP2 may play important role in determining the fate of the follicular destiny. There was no expression in normal ovarian stroma cells for c-IAP2. Conclusions : These findings suggest that c-IAP2 is expressed in ovarian carcinomas and emerging role in cancer. The c-IAP2 expression has been investigated in the normal ovary, where apoptosis is thought to play an important role in ovulation.
자궁경부암 및 난소암에서 세포자멸사 억제인자인 c-IAP1과 c-IAP2의 발현 양상
조준형,조치흠,권상훈,백원기,권건영,박준철,이정호,김종인,윤성도,차순도 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.1
목적 : 처음 baculoviruses에서 동정된 세포자멸사 억제단백들(inhibitor of apoptosis proteins;IAPS)은 바이러스가 복제하는 동안 숙주 세포의 생존을 유지하는 기능을 하고 다양한 자극에 의한 세포자멸사를 억제하는 것이 보고 되었으나 암화 과정에 관여하는 기전은 아직까지 정확히 알려져 있지 않다. 난소 암 및 자궁경부암과 그들의 정상조직에서 IAPS의 발현을 비교하여 암화과정에서의 역할을 알아보았다. 연구 방법 : 난소 암 10예와 자궁경부암 10예를 대상군으로, 정상 난소 및 양성 난소 낭종 10예, 정상 자궁 경부조직 10예를 대조군으로 사용하였다 c-lAPl, 2의 mRNA에서의 변화를 보기 위해 RT-PCR을 시행하였고 RNA의 변화를 단백질에서 검증하기 위해 Western blot을 시행하였다. 결과 . c-lAPl, 2단백질의 발현은 난소 암에서 정상 난소 조직에서 보다 높은 발현이 있었고, 정상 자궁 경부조직에서는 c-lAPl 2단백질이 전 예에서 높게 발현되었다. RNA의 변화를 단백질에서 검증하기 위해 Western blot을 시행한 결과 c-lAPl,2 단백질이 난소암에서 일관되게 높게 발현되었다. 자궁경부 조직에서의 c-lAPl 단백질의 발현은 정상 자궁경부에서 자궁경부암보다 일관된 증가를 보여 주었고, c-lAP2 단백은 RT-PCR에서 보여준 일관된 정상 자궁경부에서의 증가가 없이 자궁암에서도 증가가 나타났다. 조직에서의 정확한 발현부위를 알기 위해 면역조직화학염색을 시행하여 난소 암에서 c-lAPl 단백은 암세포의 세포질에 정확히 침착되나 정상 난소에서는 발현이 일어나지 않음을 관찰하였다 c-lAP2단백은 난소암과 양성 난소 낭종인 자궁내막종과 황체에서 발현을 보였다. c-lAPl 단백은 자궁경부암에서 암세포에서는 발현이 없었으며 주위 정상 조직에서는 발현을 나타내었고, 정상 자궁경부 조직에서도 상피세포에서 발현을 나타내었다 그러나 c-lAP2 단백은 자궁경부암이나 정상 조직에서 일관된 결과를 보이지 않았다. 결론 이상의 연구 결과로 보아 난소 암에서는 c-lAPl,2의 증가가, 자궁경부암에서는 c-lAPl의 감소가 암화 과정에 관여한다고 추정할 수 있다. Objective : The inhibitor of apoptosis proteins (IAPs) constitutes a family of highly conserved apoptosis suppressor proteins that were originally identified in baculoviruses. Although IAP homologs have recently been demonstrated to suppress apoptosis in mammalian cells, their expression and role in human gynecologic cancers are unknown. In this study auther used ovarian and cervical cancer tissues to examine the role of IAP in the regulation of apoptosis in cervical and ovarian cancers compared with normal tissues. Methods : Fresh tissues were retrieved from 10 cases each with ovarian cancers, cervical cancers and 4 normal ovarian tissues, 6 benign ovarian tumors, and 10 normal cervical tissues. Expression of mRNA was determined by RT-PCR. Western blot analysis was also used for asseessment of protein expression. Results : The overexpression of c-IAP1,2 was identified in ovarian cancers compare with normal ovaries. All cases of cervical cancer tissues were negative and normal cervical tissues were positive for c-IAP1,2 by RT-PCR assay. Using Western blot analysis, the overexpression of c-IAP1,2 was observed in ovarian cancer tissues compared with normal ovarian tissues and overexpression of c-IAP1 in normal cervical tissues. However differences were not observed with expression of c-IAP2 in cervical cancer tissues. On immunohistochemical results, the expression of c-IAP1,2 was observed in ovarian cancer tissues, normal corpus luteum, and normal cervical tissues, whereas c-IAP1 was not shown in cervical cancer tissues. There was no correlation in c-IAP2 expression between cervical cancer and normal cervical tissues. Conclusion : These results suggest that c-IAP1,2 are important elements in growth of ovarian cancers, whereas c-IAP1 may play role of down regulation to cervical carcinogenesis.