STRICTA 권고안에 따른 침 효과에 대한 무작위 대조군 임상 시험 논문의 개입 보고에 대한 평가

손지형 ( Chin Hyoung Son ),조성일 ( Sung Il Cho ),박히준 ( Hi Joon Park ),문성일 ( Sung Il Moon ) 대한경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.2

목적: Pubmed에서 검색된 침의 효과에 대한 무작위 대조군 임상시험논문에서 STRICTA 권고안의 반영률을 알아보고자 본 연구를 계획하였다. 방법: Pubmed에서 2003년 7월 1일부터 2004년 6월 30일까지 발간된 침 무작위 대조군 임상시험 논문을 검색한 후 수작업으로 침의 효과에 대해 무작위 대조군 임상시험을 실시한 36개의 논문을 채택하였다. 대상 논문들의 저널, 대상 질병에 대해 조사하였으며 Jadad scale 점수를 매기고 STRICTA 권고안의 반영률을 "STRICTA 점수"를 매겨서 알아보았다. 논문이 SCI, SCIE, 무등재된 저널 중 어디에 실려 있는가에 따라 ANOVA를 사용하여 Jadad 점수 및 STRICTA점수에 차이가 있는지 알아보았으며 단순상관 분석을 통해 STIRCTA 점수와 Jadad scale 점수의 관계를 살펴보았다. 결과: Jadad scale 점수의 평균은 2.6점 이었으며 STRICTA 점수의 평균은 11.0점 이었다. 36편의 침 무작위 임상시험논문에서 다음의 6가지 항목-문헌적 근거, 자침 깊이, 함께 처치한 내용, 시술자 배경, 참가자의 맹검여부. 대조군 선택을 정당화 할 수 있는 근거-의 기술율은 50% 미만이었다. SCI, SCIE, 무등재된 것에 따라 논문들의 Jadad 점수 평균은 통계적으로 유의한 차이를 보였으나 STRICTA 점수 평균에서는 유의한 차이를 보이지 않았다. 한편 STRICTA 점수와 Jadad scale 점수 간에는 아무런 관계가 없었다. 결론: 36편의 침 임상시험 논문은 STRICTA 권고안의 많은 부분을 받아들이고 있었으며 STRICTA 권고안을 받아들이는 정도는 Jadad 점수와는 상관이 없었다.

Antioxidant activities of Erythrina stricta Roxb.using various in vitro and ex vivo models

K AsokKumar,M UmaMaheswari,AT Sivashanmugam,V SubhadraDevi,N Subhashini,TK Ravi 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.3

Erythrina stricta, a deciduous tree widely used traditionally in indigenous system of medicine for various ailments such as rheumatism, fever, leprosy, epilepsy etc. The leaves of Erythrina stricta was extracted with ethanol (70%) and used for the evaluation of various in vitro antioxidant assays which includes H - donor activity, nitric oxide scavenging, superoxide anion scavenging, reducing ability, hydroxyl radical, hydrogen peroxide scavenging, total phenolic content, total flavonoid content, total antioxidant activity by thiocyanate and phosphomolybdenum method, metal chelating, β-carotene bleaching, total peroxy radical assays. The pro-oxidant activity was measured using bleomycin-dependent DNA damage. Ex vivo models like lipid peroxidation and erythrocyte haemolysis were also used to study the antioxidant property of the extract. The various antioxidant activities were compared with suitable standard antioxidants such as ascorbic acid, butylated hydroxyl toluene, α-tocopherol, curcumin, quercetin and Trolox. The generation of free radicals viz. O2 · -, OH·, H2O2, NO· and peroxyl radicals were effectively scavenged by the ethanolic extract of Erythrina stricta. In all the methods, the extract offered strong antioxidant activity in a concentration dependent manner. The total phenolic content, flavonoid content and total antioxidant activity in Erythrina stricta were determined as microgram (g) pyrocatechol, quercetin and α-tocopherol equivalent/mg respectively. The extract did not exhibit any prooxidant activity when compared with ascorbic acid. The results obtained in the present study clearly indicates that Erythrina stricta scavenges free radicals and reduces lipid peroxidation, ameliorating the damage imposed by oxidat Erythrina stricta, a deciduous tree widely used traditionally in indigenous system of medicine for various ailments such as rheumatism, fever, leprosy, epilepsy etc. The leaves of Erythrina stricta was extracted with ethanol (70%) and used for the evaluation of various in vitro antioxidant assays which includes H - donor activity, nitric oxide scavenging, superoxide anion scavenging, reducing ability, hydroxyl radical, hydrogen peroxide scavenging, total phenolic content, total flavonoid content, total antioxidant activity by thiocyanate and phosphomolybdenum method, metal chelating, β-carotene bleaching, total peroxy radical assays. The pro-oxidant activity was measured using bleomycin-dependent DNA damage. Ex vivo models like lipid peroxidation and erythrocyte haemolysis were also used to study the antioxidant property of the extract. The various antioxidant activities were compared with suitable standard antioxidants such as ascorbic acid, butylated hydroxyl toluene, α-tocopherol, curcumin, quercetin and Trolox. The generation of free radicals viz. O2 · -, OH·, H2O2, NO· and peroxyl radicals were effectively scavenged by the ethanolic extract of Erythrina stricta. In all the methods, the extract offered strong antioxidant activity in a concentration dependent manner. The total phenolic content, flavonoid content and total antioxidant activity in Erythrina stricta were determined as microgram (g) pyrocatechol, quercetin and α-tocopherol equivalent/mg respectively. The extract did not exhibit any prooxidant activity when compared with ascorbic acid. The results obtained in the present study clearly indicates that Erythrina stricta scavenges free radicals and reduces lipid peroxidation, ameliorating the damage imposed by oxidat

경도인지장애의 침 치료에 대한 무작위배정 대조 임상시험(RCT) 연구 논문에 대한 질 평가 : STRICTA와 CONSORT 지침을 바탕으로

신여옥,허우영,이인 대한한방내과학회 2019 大韓韓方內科學會誌 Vol.40 No.6

Objective: The purpose of this study was to investigate quality of reporting acupuncture intervention for mild cognitive impairment (MCI) based on the STRICTA and CONSORT guidelines Methods: We searched for randomized controlled trial studies of acupuncture treatment for MCI in the MEDLINE (PubMed) database. Once the online search was completed, studies were selected manually by the inclusion criteria, and the selected studies were evaluated by STRICTA and CONSORT guidelines. Results: Five studies were included. The most frequently selected points for acupuncture treatment of MCI were on the head, such as GV20, EX-HN1, GV24, and GB20. By STRICTA guidelines, all items from STRICTA were reported except three items on the checklist. By CONSORT guidelines, apart from six items which were not available, quality assessment was performed with 31 items. Eighteen items were reported by 80% of the studies. Four items were reported by 40% of the studies and another four items were reported by 20% of the studies. One item was not reported in all studies. Conclusion: Most items were reported by STRICTA guidelines, whereas many items were insufficiently reported based on CONSORT guidelines. This needs to be considered in future RCTs of acupuncture treatment for MCI.

Antioxidant activities of Erythrina stricta Roxb.using various in vitro and ex vivo models

AsokKumar, K,UmaMaheswari, M,Sivashanmugam, AT,SubhadraDevi, V,Subhashini, N,Ravi, TK Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3

Erythrina stricta, a deciduous tree widely used traditionally in indigenous system of medicine for various ailments such as rheumatism, fever, leprosy, epilepsy etc. The leaves of Erythrina stricta was extracted with ethanol (70%) and used for the evaluation of various in vitro antioxidant assays which includes H - donor activity, nitric oxide scavenging, superoxide anion scavenging, reducing ability, hydroxyl radical, hydrogen peroxide scavenging, total phenolic content, total flavonoid content, total antioxidant activity by thiocyanate and phosphomolybdenum method, metal chelating, $\beta$-carotene bleaching, total peroxy radical assays. The pro-oxidant activity was measured using bleomycin-dependent DNA damage. Ex vivo models like lipid peroxidation and erythrocyte haemolysis were also used to study the antioxidant property of the extract. The various antioxidant activities were compared with suitable standard antioxidants such as ascorbic acid, butylated hydroxyl toluene, $\alpha$-tocopherol, curcumin, quercetin and Trolox. The generation of free radicals viz. $O_2^{{\cdot}-}$, $OH^{\cdot}$, $H_2O_2$, $NO^{\cdot}$ and peroxyl radicals were effectively scavenged by the ethanolic extract of Erythrina stricta. In all the methods, the extract offered strong antioxidant activity in a concentration dependent manner. The total phenolic content, flavonoid content and total antioxidant activity in Erythrina stricta were determined as microgram (g) pyrocatechol, quercetin and $\alpha$-tocopherol equivalent/mg respectively. The extract did not exhibit any prooxidant activity when compared with ascorbic acid. The results obtained in the present study clearly indicates that Erythrina stricta scavenges free radicals and reduces lipid peroxidation, ameliorating the damage imposed by oxidative stress in different disease conditions and serve as a potential source of natural antioxidant.

견비통에 대한 침치료 임상연구의 체계적 문헌 고찰 -Jadad Scale 및 개정된 STRICTA 권고안을 중심으로-

박태현 ( Tae Hyun Park ),오창근 ( Chang Keun Oh ),박유리 ( Yu Lee Park ),양승범 ( Seung Bum Yang ),김재효 ( Jae Hyo Kim ) 대한경락경혈학회 2012 Korean Journal of Acupuncture Vol.29 No.1

Objectives : This study aims to review randomized controlled trials on acupuncture for shoulder pain according to Jadad Scale and revised STRICTA. Methods : Seven electronic databases including PUBMED, SCOPUS, RISS were searched for randomized controlled trials (RCT) of acupuncture for shoulder pain. Results : 16 RCTs were included: 14 were published in English and 2 were published in Korean. According to Jadad scale, 15 RCTs had high quality. However, most of the studies could not meet the double blindness criteria. All RCTs meet 12.9 items on average in STRICTA. Conclusions : This systematic review shows four conclusions as follows. Traditional Chinese Medicine is often used in style of acupuncture. Spots of LI15, TE14, GB21 and LI11, stainless steel needle(0.3mm×40mm), 20 retention time, manual stimulation, Deqi, and 2 times a week treatment (total 12) are often used. Double-blinded clinical trials needs to be conducted. It is controversial to use minimal acupunctures on controlled groups of RCTs on acupuncture for shoulder pain. Description about practitioner`s background needs clarifying.

극지 홍조류 Polysiphonia stricta에서 분리된 methyl trans-aconitate 유도체들과 항산화 활성

이정임,공창숙,백승오,서영완 한국해양과학기술원 2014 Ocean and Polar Research Vol.36 No.3

In our continuing study on the antioxidant activity of Polysiphonia stricta, its crude extract was fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions according to solvent polarity. The solvent fractions were evaluated for their potential to inhibit lipid peroxidation and reactive oxygen species (ROS) production in HT 1080 cells. The n-BuOH fraction most strongly inhibited both lipid peroxidation and ROS production in HT 1080 cells. The n-BuOH fraction was further separated by repeated silica gel column chromatography and RP-HPLC to give methyl aconitates (2 and 3). The chemical structure of isolated compounds was determinated by NMR spectral analysis.

STRICTA(침 임상연구에서 중재 보고를 위한 표준) 개정판: CONSORT Statement의 확충안

이향숙 ( Hyang Sook Lee ),차수진 ( Su Jin Cha ),박히준 ( Hi Joon Park ),서정철 ( Jung Chuo Seo ),박종배 ( Jong Bae J. Park ),이혜정 ( Hye Jung Lee ) 대한경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.3

Objectives and methods: The Standards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA) were published in five journals in 2001 and 2002. These guidelines, in the form of a checklist and explanations for use by authors and journal editors, were designed to improve reporting of acupuncture trials, particularly the interventions, thereby facilitating their interpretation and replication. Subsequent reviews of the application and impact of STRICTA have highlighted the value of STRICTA as well as scope for improvements and revision. To manage the revision process a collaboration between the STRICTA Group, the CONSORT Group, and the Chinese Cochrane Centre was developed in 2008. An expert panel with 47 participants was convened that provided electronic feedback on a revised draft of the checklist. At a subsequent face-to-face meeting in Freiburg, a group of 21 participants further revised the STRICTA checklist and planned dissemination. Results: The new STRICTA checklist, which is an official extension of CONSORT, includes six items and 17 sub-items. These set out reporting guidelines for the acupuncture rationale, the details of needling, the treatment regimen, other components of treatment, the practitioner background, and the control or comparator interventions. In addition, and as part of this revision process, the explanations for each item have been elaborated, and examples of good reporting for each item are provided. In addition, the word "controlled" in STRICTA is replaced by "clinical", to indicate that STRICTA is applicable to a broad range of clinical evaluation designs, including uncontrolled outcome studies and case reports. Conclusions: It is intended that the revised STRICTA, in conjunction with both the main CONSORT Statement and extension for nonpharmacologic treatment, will raise the quality of reporting of clinical trials of acupuncture.

The Assessment of Reporting Interventions in Randomized Controlled Trials of Acupuncture according to the STRICTA Recommendation

손지형,조성일,박히준,문성일,Son, Chi-Hyoung,Cho, Sung-Il,Park, Hi-Joon,Moon, Sung-Il 경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.2

목 적 : Pubmed에서 검색된 침의 효과에 대한 무작위 대조군 임상시험논문에서 STRICTA 권고안의 반영률을 알아보고자 본 연구를 계획하였다 . 방 법 : Pubmed에서 2003 년 7 월 1 일부터 2004 년 6 월 30 일까지 발간된 침 무작위 대조군 임상시험 논문을 검색 한 후 수작업으로 침의 효과에 대해 무작위 대조군 임상시험을 실시한 36 개의 논문을 채택하였다 .대상논문들의 저널, 대상 질병에 대해 조사하였으며 Jadad scale 접수를 매기고 STRICT A 권고안의 반영률을 ‘STRICTA 접수’를 매겨서 알아보았다. 논문이 SCI, SCIE, 무등재된 저널 중 어디에 실려 있는가에 따라 ANOVA 를 사용하여 Jadad 점 수 및 STRICTA 점수에 차이가 있는지 알아보았으며 단순상관 분석을 통해 STIRCT A 점수와 Jadad scale 접수의 관계를 살펴보았다. 결 과 : Jadad scale 접수의 평균은 2.6점 이었으며 STRICTA 접수의 평균은 11.0점 이었다. 36편의 침 무작위 임상시험논문에서 다음의 6가지 항목 - 문현적 근거, 자침 깊이, 함께 처치한 내용, 시술자 배경, 참가자의 맹검여부. 대조군 선택을 정당화 할 수 있는 근거-의 기술율은 50% 미만이었다. SCI, SOE, 무둥재된 것에 따라 논문들의 Jadad 접수 평균은 통계적으로 유의한 차이를 보였으나 STRICTA 점수 평균에서는 유의한 차이를 보이지 않았다. 한편 STRICTA 점수와 Jadad scale 점수 간에는 아무런 관계가 없었다. 결 론 :36 편의 침 임상시험 논문은 STRICTA 권고안의 많은 부분을 받아들이고 있었으며 STRICTA 권고안을 받아들이는 정도는 Jadad 점수와는상관이 없었다.

Evaluation of genotoxicity and mutagenicity of aqueous extracts of Rhazya stricta Decne. and Thymus vulgaris L.

Shatha Salem Hameed,M‑Zaki Mustafa ElAssouli,Ahmed M. Al-Hejin,Mohammad Zubair Alam,Sufian Mohamad ElAssouli,Faten Zubair Filimban 경희대학교 융합한의과학연구소 2018 Oriental Pharmacy and Experimental Medicine Vol.18 No.4

Traditional herbal medicines have been part of human healthcare systems since ancient times. The studies on herbal medicines have mainly focused on their beneficial aspects and hence their harmful effects have been usually overlooked. Throughout Middle East and South Asia, Rhazya stricta Decne. and Thymus vulgaris L. are used as traditional medicines for various diseases. We evaluated R. stricta and T. vulgaris aqueous extracts for their genotoxicity and mutagenicity through comet assay and Ames Salmonella mutagenicity test respectively. An overall treatment effect (p < 0.0001) showed higher genotoxicity of T. vulgaris extract indicated by increased tail moments in comet assay compared to the extract of R. stricta and the combined extract. Further, an overall dose effect (p < 0.0001) showed higher genotoxicity at 1 mg/ml which further increased at 5 mg/ml doses of the extracts compared to 0 mg/ml. The genotoxicity did not show any further increase at 10 mg/ml. The extracts were also tested for their mutagenicity through Ames Salmonella test. R. stricta and T. vulgaris were not mutagenic to TA100 and TA98 when extracts tested separately. However, combined extracts of R. stricta and T. vulgaris was weakly mutagenic to TA98 with number of revertants 92 ± 8 at the highest concentration tested. This study implies that the herbal medicines should be used cautiously as they may cause long term harmful effects along with the intended beneficial activity.

당잔대(Adenophora stricta) 뿌리의 성분연구

노태웅,윤기동 한국생약학회 2019 생약학회지 Vol.50 No.2

Adenophora stricta Miq. (Campanulaceae) is an annual herb, which has been used as a traditional medicine in Korea, Japan and China to treat bronchial asthma, tonsillitis, and hypertension. In this study, 12 compounds were isolated from the roots of A. stricta and isolates were identified to be methyl adenophorate (1), decursidin (2), L-tryptophan (3), D-1,2,3,4-tetrahydronorharmane-3-carboxylic acid (4), vanillic acid 4-O-b-D-glucopyranoside (5), syringic acid 4-O-b-D-glucopyranoside (6), vanillin (7), vanillic acid (8), p-hydroxybenzaldehyde (9), p-hydroxybenzoic acid (10), p-hydroxyacetophenone (11) and linoleic acid (12). Decursidin (2) and D-1,2,3,4-tetrahydronorharmane-3-carboxylic acid (4) is firstly reported from A. stricta in current study.