Mutation Breeding of Mushroom by Radiation

Chandran Sathesh-Prabu,이영근 한국방사선산업학회 2011 방사선산업학회지 Vol.5 No.4

Mushrooms belonging to macrofungi have been consumed by humans for their nutritionaland medicinal values for centuries throughout the world. Mushroom farming is practiced in morethan 100 countries of the world, with production increasing at a rate of 7% per annum. High yieldand good quality are always the principal goals for agriculturally important crops, including mushrooms. Several breeding methods are employed for strain improvement such as mass selection basedon the natural chance mutation and induced mutation (mutation breeding), protoplast fusion technology,cross breeding and transgenic breeding. However, mutation breeding has shown prominentsuccess in crop plant improvement. Though several-hundred mutant crop varieties have beendeveloped around the world, the mutation breeding of mushrooms is limited. This review paperexplores the potential application of radiation on the development of mutant varieties of mushroomsfor breeding with desired traits such as better quality and productivity.

한국 돌연변이육종 연구의 역사와 주요 성과 및 전망

강시용(Si-Yong Kang),김상훈(Sang Hoon Kim),류재혁(Jaihyunk Ryu),김진백(Jin-Baek Kim) 한국육종학회 2020 한국육종학회지 Vol.52 No.S

Research on mutation breeding started in the early 1960s by researchers at the Atomic Energy Research Institute, Rural Development Administration (RDA) and several universities in Korea. The Radiation Agriculture Research Institute (RARI) was established in 1966, and studies of mutation breeding using radiation were actively conducted for a while. RARI was merged into the Korea Atomic Energy Research Institute (KAERI) and RDA in 1973, and radiation breeding research was neglected by the two agencies. In the 1980s, the relevant research department was lost, which resulted in a recession period of radiation breeding research. The Advanced Radiation Research Institute (ARTI), under the KAERI, was established to promote radiation research and the industry in 2005, which led to the activation of radiation breeding research. Then, the Radiation Breeding Research Center (RBRC) at the ARTI was established with support of the Ministry of Agriculture, Food and Rural Affairs in 2013. Recently, the importance of seed and genetic resources has been emphasized in Korea, and many institutes, companies and private breeders are interested in mutation breeding. The RBRC is trying to develop advanced radiation breeding techniques and new genetic resources using mutation techniques combined with bio-tech. This is to deal with the loss of biodiversity due to global climate change and environmental degradation, growing global demand for food and bio-energy, and to strengthen the protection for new plant varieties. Approximately 180 new mutant varieties were developed and registered officially in Korea. Recently, new mutant varieties, especially of flowers and ornamental plants, have quickly increased and are being commercialized, mainly by private company and breeders.

가속기빔을 이용한 돌연변이 육종의 특성과 연구개발 현황

강시용 한국육종학회 2023 한국육종학회지 Vol.55 No.2

Mutation breeding through irradiation has been applied to several varieties and genetic resources since the discovery of the useof X-rays for inducing mutations in plants by Stadler in 1928. A heavy ion beam with high linear energy transfer (LET) shows a higherrelative biological effectiveness (RBE), and it is more effective in inducing plant mutations than low LET radiations, such as X-rays, gammarays, and electrons. Since early 1990s, several plant breeding programs in Japan have used heavy ion beams from accelerators. These beamsimpart a high energy effect on a local target; therefore, they induce a higher number of single and double strand DNA breaks. In addition,they induce a large number of DNA deletions than low LET radiations. Therefore, a heavy ion beam is superior to low LET radiations interms of induction rate and the mutation spectrum. In Korea, a heavy ion accelerator that can be used for breeding is under construction. However, a large-capacity proton accelerator (KOMAC: Korea Multi-purpose Accelerator Complex) was built recently, and it is a pioneerstep in breeding research worldwide. This review summarizes the basic characteristics, successful research achievements, and the prospectof application of high LET accelerator beams in plant mutation breeding.

Development of EMS Mutagenized Wheat Mutant Lines Resistant to Fusarium Crown Rot and Fusarium Head Blight

Kahsay Tadesse Mawcha,Dennis Ndolo,Wenxiang Yang,Olubukola Oluranti Babalola 한국육종학회 2024 Plant Breeding and Biotechnology Vol.12 No.-

Plant breeding relies on genetic variation to produce new and improved cultivars. One way to obtain novel traits is by inducing mutations. The present study aimed to create a Fusarium crown rot (FCR) and Fusarium head blight (FHB)-resistant mutagenized wheat population using ethyl methane sulphonate (EMS) and identify mutant resistance to FCR and FHB, which could provide a starting point for resistance breeding. The optimal mutagenesis conditions were determined based on the germination percentage. This study used six Chinese wheat cultivars, namely Jimai22, Hengguan35, Shixin828, Gaoyou2018, Keiwei20, and Keiwei18, to create a mutant population by treating them with EMS. For Shixin828, the optimal condition was 0.8% EMS with a 50-55% germination rate. For Hengguan35 and Jimai22, it was 0.6% EMS. For Gaoyou2018 and Kewei20, it was 0.8% and 0.4-0.6%, respectively. The FCR disease index of the mutant lines (M1) ranged from 10.00 to 77.67. For M2, the number of individual mutant plants demonstrating resistance to FCR varied from 76 to 102. In M3, 570 healthy plants were obtained using various EMS concentrations. The mutant line Kewei18 demonstrated the most resistance to FCR, FHB, and Deoxynivalenol (DON) infection. Kewei20 mutants had a higher FHB susceptibility than other mutants. Overall, mutants from the Kewei18 genetic background displayed better disease resistance to both diseases and DON contamination than natural plants. Mutants with or moderate resistance to FCR and FHB could be used in breeding and genetic studies to identify FHB and FCR-resistant Quantitative Trait Locus (QTL) in wheat.

돌연변이 육종에 의해 육성된 왜성 들잔디 품종 ‘한라그린1’

양대화,선현진,정옥철,송인자,배태웅,진일두,강홍규,고석민,권용익,송필순,이효연 한국육종학회 2016 한국육종학회지 Vol.48 No.4

To develop a dwarf turfgrass (Zoysia japonica) cultivar with artificial mutation-induced breeding method, the wild type control "Gosan" plants were exposed to a 30 Gy gamma ray source in 2010. The mutant lines showing short height were selected from successive generations. One of the resulting dwarf lines obtained was registered under the cultivar name of “Halla Green 1” (2016). The dwarf phenotype of the Halla Green 1 includes a reduction of the height by 4.5-fold, an increase in leaf and third internode lengths by about 6- and 2.3-fold, respectively, compared to the Gosan, and approximately 2.4-, 3.8-, and 1.5-fold relative to the Zenith, respectively. In addition, the Halla Green 1 had a sheath of darker green coloring compared to the light green Gosan and Zenith. The leaf blades of Gosan, Zenith and Halla Green 1 were all light green, whereas their stolons were purple, yellow-green and light purple, respectively. Trichomes presented on both adaxial and abaxial surfaces of the Gosan’s leaves, and only on the adaxial side of the Zenith’s leaves, but none on the Halla Green 1 leaves. The Halla Green 1 exhibited sufficiently distinct morphological traits when compared with the wild type Gosan and Zenith that the dwarf phenotype enhances its commercial viability.

Effect of gamma irradiation on the morphological and physiological variation from In vitro individual shoot of banana cv. Tanduk (Musa spp.)

Ferid Abdulhafiz,Fatimah Kayat,Suhana Zakaria 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.2

Inducing genetic and morphological variation through conventional method is very difficult. Therefore, mutation induction through in vitro technology brings numerous advantages over the conventional breeding. Thus, the individual shoots (1 ~ 2 cm) were irradiated with gamma rays (10 ~ 70 Gy). The result revealed that the explants treated with higher doses (40, 50, 60, and 70 Gy) showed deleterious effects of ionizing radiation. The highest survival rate among γ treated explants recorded was 71% in 10 Gy treatments while the lowest survivality was 15% in 70 Gy. Lethal dose 50% (LD50) dose was found to be 33 Gy. In the in vitro condition, rooting reponse showed that increase in gamma irradiation dose resulted in the inhibition of root growth. Meanwhile, non-treated explants had the best rooting ability with the maximum number of root per explant (20) within a short period of time (6 days), with the highest root length of (15.1 cm). The longer period in rooting (12 days) and lowest number of root per explant (8) with shortest root length (10.1 cm) were recorded at 30 Gy treatment. The highest shoot length (13.6 cm) was observed at control treatment and the shortest shoot length (10.4 cm) was observed at 30 Gy. In the nursery, lowest leaf number (5) was observed at 30 Gy compared with other treatments. The highest chlorophyll content (49.8) was recorded at 10 Gy treated seedling. Irradiated explants with 10 Gy found to be superior over the control treatment and had positive effects in main growth parameters such as chlorophyll content.

Effect of gamma irradiation on the morphological and physiological variation from In vitro individual shoot of banana cv. Tanduk (Musa spp.)

Abdulhafiz, Ferid,Kayat, Fatimah,Zakaria, Suhana The Korean Society of Plant Biotechnology 2018 식물생명공학회지 Vol.45 No.2

Inducing genetic and morphological variation through conventional method is very difficult. Therefore, mutation induction through in vitro technology brings numerous advantages over the conventional breeding. Thus, the individual shoots (1 ~ 2 cm) were irradiated with gamma rays (10 ~ 70 Gy). The result revealed that the explants treated with higher doses (40, 50, 60, and 70 Gy) showed deleterious effects of ionizing radiation. The highest survival rate among ${\gamma}$ treated explants recorded was 71% in 10 Gy treatments while the lowest survivality was 15% in 70 Gy. Lethal dose 50% ($LD_{50}$) dose was found to be 33 Gy. In the in vitro condition, rooting reponse showed that increase in gamma irradiation dose resulted in the inhibition of root growth. Meanwhile, non-treated explants had the best rooting ability with the maximum number of root per explant (20) within a short period of time (6 days), with the highest root length of (15.1 cm). The longer period in rooting (12 days) and lowest number of root per explant (8) with shortest root length (10.1 cm) were recorded at 30 Gy treatment. The highest shoot length (13.6 cm) was observed at control treatment and the shortest shoot length (10.4 cm) was observed at 30 Gy. In the nursery, lowest leaf number (5) was observed at 30 Gy compared with other treatments. The highest chlorophyll content (49.8) was recorded at 10 Gy treated seedling. Irradiated explants with 10 Gy found to be superior over the control treatment and had positive effects in main growth parameters such as chlorophyll content.

겹꽃 분화용 할미꽃 신품종 "겨비"

이야성,김동관,최덕수,최진경,손동모,최경주,백형진,임요섭 한국육종학회 2010 한국육종학회지 Vol.42 No.3

할미꽃 신품종 ‘겨비’는 전라남도농업기술원에서 2001년에 자생 할미꽃(Pulsatilla koreana) 종자를 화학적 돌연변이를 유발(sodium azide 3 mM/16 hr)하고, 2004년과 2005년에 특성검정을 통해 2005년에 육성되었다. ‘겨비’의 꽃은 적자색이고 약은 진노랑, 주두는 적자색이며 화형은 겹꽃으로 꽃잎이 12매이다. 주당 화경은 6.3개, 화경장과 엽장은 각각 26.4, 14.2 cm이고, 화폭과 포폭은 각각 7.5, 11 A new pasqueflower (Pulsatilla koreana) variety, ‘Gyeobi,’ was derived from a mutation of native P. koreana at the Jeollanamdo Agricultural Research and Extension Services (JARES) in Naju, Korea. The ‘Gyoebi’ was obtained by treating seeds with 3 mM of a chemical mutagen, sodium azide, for 16 hr in 2001. The variety was established in 2005 after two years of selective breeding. ‘Gyeobi’ is characterized by reddish purple flowers with 12 double petals. The inherent characteristics of the variety are deep yellow anthers and reddish purple stigmas. The agronomic characteristics of the variety are 6.3 flowers per plant, 26.4 cm in flower height, 14.2 cm in leaf length, 7.5 cm in flower width, and 11.0 cm in bract width.

UV-ARTP-DES compound mutagenesis breeding improves natamycin production of Streptomyces natalensis HW-2 and reveals transcriptional changes by RNA-seq

Jianrui Sun,Jinglan Li,Linlin Yao,Yingying Zheng,Jiang-Feng Yuan,Da-Hong Wang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.3

Natamycin is widely used in food, medical and health, agriculture, and animal husbandry. In this study, Streptomyces natalensis HW-2 was used as the research object, and a mutant DES-26 with stable genetic characters was selected by UV-ARTP-DES compound mutation. The natamycin yield was 1.64 g/L, 86.36% higher than original strain. Differential expression genes were analyzed by transcriptomics, and results showed that 295 and 860 genes were significantly differentially expressed at fermentation for 48 h and 72 h. GO and KEGG analysis showed that compound mutagenesis had a significant impact on glycolysis, pentose phosphate, TCA cycle, fatty acid metabolism pathways, and several key enzyme genes in the pathways were up-regulated, and genes related to natamycin biosynthesis (pimB-pimI) and transcriptional regulator (pimR) were also up-regulated. qRT-PCR results confirmed that expression levels of these genes were consistent with transcriptional changes of RNA-Seq.

Sensitivity of Lavender to Proton, Electron, and Gamma Radiation

Wensheng Chen,Hui Li,Lei Shi,Hong Tong Bai 한국원예학회 2016 원예과학기술지 Vol.34 No.1

While ion beams are widely used in plant breeding, little is known about the sensitivity of Lavandula angustifolia (lavender) to ionizing radiation. To compare the biological effects of different types of ionizing radiation on the germination and survival rates of lavender, we exposed lavender seeds to gamma rays, 3 MeV electron beams, and 1.89 MeV proton ion beams. We observed that the seed germination rate decreased with increasing dosages of all three types of ionizing radiation. The malformation rate of lavender seedlings exposed to electron beams and gamma rays increased with increasing radiation dosage. By contrast, the effect of the accelerated proton beams on the malformation rate was negatively correlated with the dosage used. The survival rate of lavender seedlings exposed to the three types of ionizing radiation decreased in a dose-dependent manner. In addition, the survival rate of seedlings irradiated with proton and electron beams decreased more slowly than did that of seedlings irradiated with gamma rays. The half-lethal dose of gamma rays, electron beams, and proton beams was determined to be 48.1 Gy, 134.3 Gy, and 277.8 Gy, respectively, and the most suitable proton-ion energy for lavender seeds in terms of penetration depth was determined to be 5 MeV. These findings provide valuable information for the breeding of lavender by radiation mutation.