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Investigation of Enzymatic Activities in Marine Algae-Derived Fungi
Chung Dawoon,Yu Woon-Jong,Jang Hyeong Seok,Kwon Yong-Min,Bae Seung Seob,Choi Grace (사)한국해양생명과학회 2023 한국해양생명과학회지 Vol.8 No.1
Marine macroalgae are important in coastal ecosystems and interact with marine microorganisms. In this study, we isolated fungi from seven types of marine macroalgae including Cladophora sp., Gloiopeltis furcate, Gracilariopsis chorda, Hydroclathrus clathratus, Prionitis crispata, Sargassum micracanthum, and Ulva lactuca collected in Korea. Morphological and phylogenetic analyses identified the isolates as four Aspergillus spp. (A. fumigatus, A. sydowii, A. tamarii, and A. terreus), three Penicillium spp. (P. crustosum, P. jejuense, and P. rubens), and Cladosporium tenuissimum. Among them, A. fumigatus TOP- U2, A. tamarii SH-Sw5, and A. terreus GJ-Gf2 strains showed the activities of all enzymes examined (amylase, chitinase, lipase, and protease). Based on the enzymatic index (EI) values in solid media, A. terreus GJ-Gf2 and C. tenuissimum UL-Pr1 exhibited the highest amylase and lipase activities, respectively. Chitinolytic activity was only observed in A. terreus GJ-Gf2, A. tamarii SH-Sw5, and A. fumigatus TOP-U2. Penicillium crustosum UL- Cl2 and C. tenuissimum UL-Pr1 showed the highest protease activities. To the best of our knowledge, this is the first report of lipolytic and proteolytic activities in a marine-derived C. tenuissimum strain. Overall, the fungal strains isolated from the marine macroalgae in this study actively produced industrially important enzymes.
Rui Liu,Qian-Qun Gu,Wei-Ming Zhu,Cheng-Bin Cui,Guo-Tao Fan 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.9
Two known modified dipeptides, trichodermamide A (1) and aspergillazine A (2), were isolated from an ethyl acetate extract of the metabolite of a marine-derived fungus Spicaria elegans, and were found to have a weak cytotoxic effect on three cancer cell lines P388, A-549, and HL-60 agreed. To our knowledge, this is the first report on the isolation of compounds 1 and 2 from the fungus Spicaria elegans and their cytotoxic effect.
Yun, Keumja,Kondempudi, Chinni Mahesh,Choi, Hong Dae,Kang, Jung Sook,Son, Byeng Wha The Pharmaceutical Society of Japan 2011 Chemical & pharmaceutical bulletin Vol.59 No.4
<P>The biological transformation of the biologically active chlorogentisyl alcohol (<B>1</B>), isolated from the marine-derived fungus <I>Aspergillus</I> sp., was studied. Preparative-scale fermentation of chlorogentisyl alcohol with marine-derived fungus <I>Chrysosporium synchronum</I> resulted in the isolation of a new glycosidic metabolite, 1-<I>O</I>-(α-<SMALL>D</SMALL>-mannopyranosyl)chlorogentisyl alcohol (<B>2</B>). The stereostructure of the new metabolite obtained was assigned on the basis of detailed spectroscopic data analyses, chemical reaction, and chemical synthesis. Compounds <B>1</B> and <B>2</B> exhibited significant radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) with IC<SUB>50</SUB> values of 1.0 and 4.7 μ<SMALL>M</SMALL>, respectively. The compounds <B>1</B> and <B>2</B> were more active than the positive control, <SMALL>L</SMALL>-ascorbic acid (IC<SUB>50</SUB>, 20.0 μ<SMALL>M</SMALL>).</P>
Velmurugan, Natarajan,Lee, Hyun-Mi,Cha, Hyo-Jung,Lee, Yang-Soo De Gruyter 2017 Botanica marina Vol.60 No.4
<P>An efficient and unique proteomic response is important for fungi to survive in marine environments polluted with polycyclic aromatic hydrocarbons (PAHs). The marine-derived fungus Paecilomyces sp. strain SF-8 was isolated from the soil of salt production sites and was found to remove different PAHs. The PAH-treated strain SF-8 was characterized using elemental analysis and Fourier transform infrared spectroscopy. Paecilomyces sp. strain SF-8 successfully removed 88% of anthracene, 75% of phenanthrene, 67.5% of benzo[a] anthracene, and 99.3% of benzo[b] fluoranthene. In order to gain insight into the molecular response to PAHs in strain SF-8, proteomics analysis of different cells treated with different PAHs was performed. Comprehensive proteomic analysis revealed that the molecular response of the fungus towards PAH was compound specific. Functional classifications showed heightened regulation of major enzymes involved in cellular function and metabolism, and PAH degradation. The key control protein (salicylaldehyde dehydrogenase) of PAH degradation was specifically over-expressed. Our results collectively demonstrated the influence of PAHs in fungal cellular functioning, development and metabolism.</P>
Protulactones A and B: Two New Polyketides from the Marine-derived Fungus Aspergillus sp. SF-5044
Sohn, Jae-Hak,Oh, Hyun-Cheol Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.6
Protulactones A (1) and B (2), two new polyketide-derived fungal metabolites, have been isolated from an EtOAc extract of the marine-derived fungus Aspergillus sp. SF-5044 by various chromatographic methods. The structures of 1 and 2 were mainly determined by analysis of the NMR spectroscopic data and MS data, along with chemical methods such as Mosher method. Protulactones A (1) and B (2) are new members of polyketide-derived secondary metabolites, possessing unique ring systems among the fungal metabolites produced by the genus Aspergillus.
Griseusin C, a Novel Quinone Derivative from a Marine-Derived Fungus Penicillium sp.
Li, Xiang,Zheng, Yinan,Sattler, Isabel,Lin, Wenhan The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.11
A novel quinone derivative, Griseusin C (1), along with a known quinone, Naphthoquinone C (2), was isolated from the lyophilized culture broth of the marine-derived fungus Penicillium sp. The structures were elucidated on the basis of extensive 1D-and 2D-NMR, as well as HRESIMS, spectroscopic analysis. The relative stereochemistries of the compounds were assessed by NOESY analysis.
Long-Term Investigation of Marine-Derived Aspergillus Diversity in the Republic of Korea
Jun Won Lee,Wonjun Lee,Rekhani Hansika Perera,Young Woon Lim 한국균학회 2023 Mycobiology Vol.51 No.6
Aspergillus species play a crucial role in terrestrial environments as degraders and are wellknown for producing various secondary metabolites. Recently, Aspergillus species have beendiscovered in marine environments, exhibiting adaptability to high salinity and producingdiverse secondary metabolites with valuable properties. However, limited research hasfocused on their marine diversity, leading to inaccurate species identification. The currentstudy addresses this gap by investigating diverse marine habitats in the Republic of Korea,including sediment, seawater, seaweed, and marine animals. From three coasts of theKorean Peninsula, 472 Aspergillus strains were isolated from the various marine habitats. Atotal of 41 species were accurately identified using multigenetic markers: internal transcribedspacer, calmodulin, and b-tubulin. The findings underscore the importance of accurate identificationand provide a basis for elucidating the functional role of marine-derived Aspergillusspecies in marine ecosystems.
Griseusin C, a Novel Quinone Derivative from a Marine-Derived Fungus Penicillium sp.
Xiang Li,Yinan Zheng,Isabel Sattler,Wenhan Lin 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.11
A novel quinone derivative, Griseusin C (1), along with a known quinone, Naphthoquinone C (2), was isolated from the lyophilized culture broth of the marine-derived fungus Penicillium sp. The structures were elucidated on the basis of extensive 1D- and 2D-NMR, as well as HRESIMS, spectroscopic analysis. The relative stereochemistries of the compounds were assessed by NOESY analysis.
Cephalosporolides H and I, Two Novel Lactones from a Marine-Derived Fungus, Penicillium sp.
Xiang Li,Yanhua Yao,Yinan Zheng,Isabel Sattler,Wenhan Lin 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7
Two novel lactones, Cephalosporolides H (1) and I (2), were isolated from the lyophilized culture broth of the marine-derived fungus, Penicillium sp. The structures were elucidated on the basis of extensive 1D- and 2D-NMR, as well as HRESI-MS spectroscopic analyses. The relative stereochemistries of the compounds were assessed by comparison of the NOESY analysis and spectral data with those in the literature.
Nguyen Thi Thanh Ngan,TRANHONG QUANG,김관우,김혜진,손재학,강대길,이호섭,김윤철,오현철 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.3
After the chemical investigation of the ethylacetate extract of the marine-derived fungal strain Penicilliumsp. SF-5629, the isolation and structural elucidationof eight secondary metabolites, including (3R,4S)-6,8-dihydroxy-3,4,7-trimethylisocoumarin (1), (3S,4S)-sclerotininA (2), penicitrinone A (3), citrinin H1 (4), emodin (5),x-hydroxyemodin (6), 8-hydroxy-6-methyl-9-oxo-9Hxanthene-1-carboxylate (7), and 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8) were carried out. Evaluation of the anti-inflammatory activity of thesemetabolites showed that 4 inhibited nitric oxide and prostaglandinE2 production in lipopolysaccharide-stimulatedBV2 microglia, with IC50 values of 8.1 ± 1.9 and8.0 ± 2.8 lM, respectively. The inhibitory function of 4was confirmed based on decreases in inducible nitric oxidesynthesis and cyclooxygenase-2 gene expression. In addition,4 was found to suppress the phosphorylation ofinhibitor kappa B-a, interrupt the nuclear translocation ofnuclear factor kappa B, and decrease the activation of p38mitogen-activated protein kinase.