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한국인 자궁경부암 조직에서 분리한 HPV16형 E6 유전자의 대장균에서의 발현
이영희,진승원,윤희식,박병철,임종석,이희구,정현호,최인성,박순희 대한바이러스학회 1995 Journal of Bacteriology and Virology Vol.25 No.2
Human papillomaviruses, especially type 16 and 18, have been reported to be closely associated with human cervical lesions and carcinoma. One of the open reading frames, E6 has been identified as a transforming gene. We have isolated the HPV16 E6 oncogene from Korean cervical carcinoma tissues, subcloned into an expression plasmid pET-3a, and produced 16E6 proteins in E. coli under the control of T7 promoter. Recombinat E6 protein was successfully produced in high level upto 30% of total proteins and specifically recognized by anti-16E6 polyclonal antibodies produced by rabbit immunized with TrpE-16E6 fusion protein. 16E6 protein was produced in insoluble form. Currently we are establishing purification procedure of solubilized E6 protein.
생기액(生肌液)의 세포독성 및 자궁경부암 바이러스 (HPV 16 type) 암 유발인자 E6와 E7의 작용에 미치는 효과
정옥(Ok Joung),조영식(Young Sik Cho),조정원(Cheong Weon Cho),이경애(Kyung Ae Lee),심정현(Jung Hyun Shim),조민철(Min Chul Cho),이홍수(Hong Soo Lee),염영일(Young Il Yeom),김상범(Sang Bom Kim),박순희(Sue Nie Park),윤도영(Do Young Yoon) 대한약학회 2000 약학회지 Vol.44 No.4
Cervical cancer is one of the leading causes of female death from cancer worldwide with about 500,000 deaths per year. A strong association between certain human papilloma viruses (HPV types 16 and 18) and cervical cancer has been well known. An extract of natural products, named as Somatid, has been used to investigate whether this agent has the ability of inhibiting the oncogenes E6 and E7 of HPV type 16. This Somatid inhibited the proliferation of human cervical cancer cell lines (C-33A, SiHa, CaSki) and HaCaT keratinocytes in a dose response manner. In vitro binding assay and ELISA showed that Somatid inhibited the in vitro biding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53. In addition, Somatid inhibited the in vitro binding of E7 and Rb which is essential tumor suppressor for the control of cell cycle. The levels of mRNA for E6 and E7 were also decreased by Somatid. Our data suggested that Somatid inhibited the oncogenecity of E6 and E7 of HPV 16 type, thus can be used as a putative anti-HPV agent for the treatment of cervical carcinomas caused by HPV.
정연구(Yeon Gu Chung),이해숙(Hae Sook Lee),이경애(Kyung Ae Lee),정옥(Ok Joung),오원근(Won Keun Oh),김광동(Kwang Dong Kim),임종석(Jong Seok Lim),문자영(Ja Young Moon),조용권(Yong Kweon Cho),박순희(Sue Nie Park),윤도영(Do Young Yoon) 대한약학회 2002 약학회지 Vol.46 No.6
Cervical cancer is one of the leading causes of female death from cancer worldwide with about 500,000 deaths per year, A strong association between certain human papilloma viruses (HPV type 16 and 18) and cervical cancer has been well known. An extract of Saururus chinensis, named as PE-46, has been used to investigate whether this agent has the ability of inhibiting the oncogenes E6 and E7 of HPV type 16, PE46 inhibited the proliferation of human cervical cancer cell lines in a dose response manner, PE46 also inhibited the in vitro binding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53. In addition, PE-46 inhibited the in vitro binding of E7 and Rb which is essential tumor suppressor for the control of cell cycle. The levels of MRNA for E6 and E7 were also decreased by PE-46. SiHa cells treated with PE46 induced GO/G1 arrest, resulting in inhibition of growth. Our study showed that the PE-46 can inhibit the cervical carcinomas via both inhibition of bindings between oncogenes and tumor suppressors, and inhibition of G1->S S transition. PE-46 inhibited the oncogenecity of E6 and E7 of HPV 16 type, thus could be used as a putative modulating agent for the treaoent of cervical Carcinomas caused by HPV.
조정남 ( Jung Nam Cho ),윤소연 ( So Yeon Yoon ),현대성 ( Dae Sung Hyun ) 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.71 No.5
Background: Lung cancer is the leading cause of cancer deaths in the world. Human papillomavirus (HPV) infection and E6 oncoprotein expression are known risk factors for the development of non-small cell lung cancer (NSCLC). This study was performed to evaluate the prevalence of HPV 16/18 E6 oncoprotein expression in patients with NSCLC. Methods: Immunohistochemical stains of the HPV 16/18 E6 oncoprotein were performed in tumor tissues from 68 patients with NSCLC who underwent curative surgery from March 2006 to November 2008. Results: The E6 oncoprotein was expressed in 29.4% of patients with NSCLC and a statistical analysis revealed that E6 oncoprotein expression was significantly higher in females (p=0.028), never smokers (p=0.045), and patients with adenocarcinoma (p=0.022) than that in other patients. Conclusion: The E6 oncoprotein was expressed in 29.4% of patients with NSCLC. Further studies detecting HPV infection and E6 oncoprotein expression in never smoking patients with NSCLC are needed.
In situ Hybridization법과 중합효소 연쇄반응법을 이용한 한국여성 자궁경부내 Papilloma 바이러스 감염의 검색
권두환,이영희,이동화,차상헌,최용경,박순희,최인성,정태화 대한바이러스학회 1993 Journal of Bacteriology and Virology Vol.23 No.1
The relationship of certain types of human papillomavirus(HPV) infections and cervical cancers has been strongly confined. HPV types such as 6b and 11 are associated with benign tumors such as condylomas, while HPV types 16, 18, and occasionally, 31, 33, 51 types are assoeiated with cervical intraepithelial neoplasia(CIN) and carcinoma. Especially HPV 16 and 18 types are frequently found in malignan tleisons of cervical tissues. In this study, we examined and compared the prevalence of several types of HPVs in cervical tissues frorn Korean women using in situ hybridzation (ISH) and DNA polymerase chain reaction (PCR). Fifty cases of paraffin-embedded in only one of 14 cases with low and high grade CINs, HPV 16/18 types in 3(21% ) of 14 with low and high grade CINs, 6(24%) of 25 with CIS, and none of 4 with invasive carcinoma, and HPV types 31/33/51 in 2(14%) of 14 with low and high grade CIN a, 6(25 %) of 25 with CIS, and none of 4 with invasive carcinoma by ISH. Fifty-six cases of paraffin-embedded tissues and another 10 more tissues stored in liquid nitrogen were analyzed by PCR. When the presence of HPV 16 genome was examined by the amplification of 16 type E6 oncogene, 37/55(67%) and 6(60% ) cases were positive for paraffin-embedded tissues and frozen tissues, respectively. When the positivity for the presence of HPV 16 E6 genome in thirty-six tissues processed both for ISH, 9/36 (25% ) and PCR, 26/36 (72% ) methods was compared, the latter was extremely sensitive. When the presence of HPV Ll gene coding for major capsid antigen was amplified by using Ll consensus amplimers for paraffin embedded tissues, 28 samples of 45(62%) were found to be positive, 21 of them were HPV 16-positive, while for frozen tissues 6 of 10 were HPV 16-positive.This implies that other types of HPVs are presentin 7 and 2 cases of them, respectively. The amplified DNAs by PCR were confirmed by Southern blot hybridization using either P-labelled of biotin-labelled probe.