공기 중 저급아민류의 분석과 측정 사례

양성봉,유미선 한국분석과학회 2005 분석과학 Vol.18 No.3

In this study, several kinds of volatile amines in ambiant air were collected and their concentrations were expected from the calibration curves prepared by standard solutions of 7 amines by the official measuring method prescribed in Japanese Offensive Odor Law. The obtained calibration curves showed a good linearity and the detection limit of trimethyl amine was found to be about 0.033 ppb (0.040 ng) in case of 50 liters air. It means that trimethyl amine could be detected with the concentration of lower than its permitting level at the border line of companies. As typical examples of measuring amines in air, results of investigation of two sewage works and one rendering plant around the capital area indicated that 4 kinds of amines, i.e. methyl amine, dimethyl amine, trimethyl amine and isopropyl amine were detectable and concentrations of trimethyl amine at the primary sedimentation pond and sludge dewatering building of the sewage plant A showed 9.07 ppb and 7.79 ppb respectively, being over the concentration of odor strength 2.5, And the aeration tank, excrement input facility and indoor of maintenance room of excrement process building in the sewage plant B showed 70.0 ppb of dimethyl amine and 2.44 ppb of trimethyl amine.

Electrochemical nitric oxide biosensor based on amine-modified MoS₂/graphene oxide/myoglobin hybrid

Yoon, Jinho,Shin, Jae-Wook,Lim, Joungpyo,Mohammadniaei, Mohsen,Bharate Bapurao, G.,Lee, Taek,Choi, Jeong-Woo Elsevier 2017 Colloids and Surfaces B Vol. No.

<P><B>Abstract</B></P> <P>Nitric oxide (NO) is one of the most important molecules in living things due to its role as a signaling molecule in influencing pathological and physiological mechanisms including neurotransmission. In this study, the electrochemical biosensor based on the amine-modified molybdenum disulfide nanoparticles (MoS<SUB>2</SUB>), graphene oxide (GO) and myoglobin (Mb) hybrid material (amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid) is developed to achieve the accurate detection of NO with electrochemical signal improvement. For the first time, the synthesis of MoS<SUB>2</SUB> accompanying the amine-modification of the surface of MoS<SUB>2</SUB> is done to hybridize with GO efficiently through the short linkage. After the amine-modification of MoS<SUB>2</SUB>, it is enclosed with GO directly (amine-modified MoS<SUB>2</SUB>/GO). Then, Mb which can induce the reduction of NO is immobilized on the amine-modified MoS<SUB>2</SUB>/GO to fabricate the amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid for NO detection. The prepared hybrid shows the signal improved redox properties relative to the result of the electrode prepared without hybrid. Furthermore, upon addition of NO, the electrode prepared with hybrid shows the improved amperometric response compared with that of the electrode without hybrid. This amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid can be used in the development of the biosensor platform accompanying the electrochemical signal improvement and accurate detection of target materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The nitric oxide biosensor was developed by amine-modified MoS<SUB>2</SUB>/GO/Mb hybrid. </LI> <LI> For the first time, synthesis of MoS<SUB>2</SUB> accompanying the amine-modification was done. </LI> <LI> The hybrid showed the increase of electrochemical signal and amperometric response. </LI> <LI> The hybrid showed high selectivity in nitric oxide detection. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

청국장 중 biogenic amine의 함량 및 생성원인

한규홍(Gyu-Hong Han),조태용(Tae-Yong Cho),유명상(Myung-Sang Yoo),김천수(Chun-Soo Kim),김정민(Jung-Min Kim),김현아(Hyun-Ah Kim),김미옥(Mi-Ok Kim),김성철(Seong-Cheol-Kim),이선애(Sun-Ae Lee),고용석(Yong-Suk Ko),김소희(So-Hee Kim),김대병(Da 한국식품과학회 2007 한국식품과학회지 Vol.39 No.5

본 연구에서는 청국장에서 biogenic amine의 함량을 조사하고, 이를 생성하는 미생물의 스크리닝 및 biogenic amine 형성 등을 통하여 생성원인을 규명하고자 하였다. 그 결과 국내 시판 청국장에는 putrescine, histamine, tryptamine 등 11종의 biogenic amine이 검출되었으며, 이 중 tyramine이 4.2±0.9-483.1±28.4 mg/kg의 범위를 보여주여 다른 amine에 비하여 높게 나타났다. 청국장내 균주는 B. amyloliquefaciens, B. subtilis, B. licheniformis 등이 분리?동정되었고, 이들 균주에 대한 탈탄산 효소 활성 스크리닝을 살펴본 결과 모두 양성(+) 반응을 보여주었다. 최종적으로 균주에 대한 biogenic amine 생성을 확인하였을 때, 청국장내 존재하는 균주가 탈탄산 효소 활성을 통하여 biogenic amine 생성하는 것을 확인할 수 있었다. The potential to produce biogenic amines was investigated in microbial strains isolated from fermented soybean paste, cheonggukjang. The typical levels of 11 biogenic amines, including putrescine, histamine, and tryptamine, were analyzed in commercial cheonggukjang. The tyramine and histamine levels in the samples ranged from 4.2 to 483.1 mg kg^-1 and 0.2 to 70.3 mg kg?1, respectively. A total of 4 microbial strains of Bacillus sp. were isolated from cheonggukjang. The Bacillus sp. were identified as B. amyloliquefaciens, B. subtilis, and B. licheniformis based on phenotypic characteristics, which included using the VITEK system. The screening plate method for detecting amino acid decarboxylase positive microorganisms was performed. The results for amine decarboxylation were positive, and biogenic amine formation was evaluated by the confirmation of amine-forming capacity.

아민 차수에 따른 고체 흡수제의 이산화탄소 분리 특성

장현태 한국화학공학회 2023 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.61 No.4

Primary and secondary amine-based sorbents were synthesized to investigate the operation capacity for the carbon dioxide separation TSA process. (3-Aminopropyl) triethoxysilane was used as a primary amine precursor as a crosslinking agent to synthesize a secondary amine precursor in which amine groups were crosslinked with a crosslinking agent. Carbon dioxide absorbed by primary amines is completely separated above 170 °C. The working capacity of the primary amine absorbent was less than 2% when regenerated at 130°C. The secondary amine absorbent has a higher carbon dioxide separation capacity at a lower regeneration temperature than the primary amine absorbent. The secondary amine absorbent could predict process operation performance of about 6.5% with 2% carbon dioxide absorption and 100% carbon dioxide regeneration conditions. Therefore, it was found that the working capacity of the secondary amine absorbent was higher than that of the primary amine.

Cu²⁺ sequestration by amine-functionalized silica nanotubes

Ko, Y.G.,Lee, H.J.,Oh, H.C.,Choi, U.S. Elsevier Scientific Pub. Co 2013 Journal of hazardous materials Vol.260 No.-

A novel method for Cu<SUP>2+</SUP> sequestration in Cu<SUP>2+</SUP> aqueous solution has been demonstrated using amine-functionalized double-walled silica nanotubes (DWSNTs). Herein, the precipitation method and the adsorption method are combined to remove Cu<SUP>2+</SUP> in the Cu<SUP>2+</SUP> aqueous solution. Primary (1<SUP>o</SUP>), secondary (2<SUP>o</SUP>), tertiary (3<SUP>o</SUP>), di-, tri-amines are immobilized on the surface of DWSNT as the adsorption site. The results show that the Cu<SUP>2+</SUP> adsorption amount on the amine-functionalized DWSNTs is in the following order: tri-amine>di-amine>1<SUP>o</SUP> amine>2<SUP>o</SUP> amine>3<SUP>o</SUP> amine. The complexed Cu<SUP>2+</SUP>s with the amine-functionalized DWSNTs become Cu(OH)<SUB>2</SUB> crystals due to the reaction with OH<SUP>-</SUP>s dissociated from water. Thus, the amine-functionalized DWSNTs show the superior sequestration capacity of Cu<SUP>2+</SUP> in the Cu<SUP>2+</SUP> aqueous solution owing to the Cu(OH)<SUB>2</SUB> crystals growth on them. FT-IR, FEG-SEM, HR-TEM, and XRD studies demonstrate the mechanism of the Cu<SUP>2+</SUP> adsorption and the Cu(OH)<SUB>2</SUB> crystals growth. The crystallization-technique of the heavy metal ion on the amine-functionalized DWSNTs is also expected to have potential applications such as the facile synthesis of nano- and microparticles, and the metal catalyst supporter.

Amine-enriched surface modification facilitates expansion, attachment, and maintenance of human cardiac-derived c-kit positive progenitor cells

Choi, S.H.,Jung, S.Y.,Yoo, S.M.,Asahara, T.,Suh, W.,Kwon, S.M.,Baek, S.H. Elsevier/North-Holland Biomedical Press 2013 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.168 No.1

Background: Stem cells have a low expansion rate and are difficult to maintain in vitro. To overcome the problems of cardiovascular regeneration, we developed a novel method of stem cell cultivation in culture vessels with amine and carboxyl coatings. Methods and results: We isolated cardiac stem/progenitor cells from infant-derived heart tissue by using c-kit antibody (human cardiac-derived c-kit positive progenitor cells; hCPC<SUP>c-kit+</SUP>); the cells differentiated into endothelial cells, smooth muscle cells, and cardiomyocytes. To characterize the effect of surface modification on hCPC<SUP>c-kit+</SUP> expansion, cellular attachment, c-kit expression maintenance, and cardiomyocyte differentiation, we tested hCPC<SUP>c-kit+</SUP> cultured on non-coated (control), amine-coated (amine), and carboxyl-coated (carboxyl) vessels. Ex vivo proliferation, c-kit maintenance, and cellular attachment were significantly enhanced in the amine group. The amine coating also increased procollagen type I (pro-COL1) expression and increased phosphorylation signals, such as focal adhesion kinase (FAK) and cytosolic Src, as well as enhanced ERK/CDK2 signaling. In addition, there was significant downregulation of the stress signal transducer, JNK, in the amine group. However, cardiomyogenesis remained unchanged in the control, amine, and carboxyl groups. Conclusions: Although surface modifications had no effect on early induction cardiomyogenesis, amine-enriched surface modification may increase hCPC<SUP>c-kit+</SUP> expansion. The amine-enriched surface improved cellular proliferation and attachment during ex vivo hCPC<SUP>c-kit+</SUP> expansion, possibly by modulating intracellular signal transducers.

Effect of Amine Loading on Free Fatty Acid Adsorption of Amine-functionalized Mesoporous Silica

( Wang Yu ),곽승엽 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1

Extra virgin olive oil (EVOO) is recognized as the highest grade of olive oil. However free fatty acid (FFA) in EVOO as a strong pro-oxidant promotes rancidity and lowers thermal stability. Therefore it is essential to remove FFA from EVOO. Adsorptive removal has attracted considerable attention. One promising adsorbent is mesoporous silica (MS) result from its large pore volume and surface area, controllable pore size, and facile surface modification. Especially adsorptive applications of MS are dependent on its surface properties. We report that amine surface functionalization of MS is used to improve its adsorption capacity and selectivity for FFA. Besides amine loading greatly affects interaction strength and space for FFA adsorption. Amine-functionalized MS with different amine loading were prepared and adsorption performance of resulting samples was evaluated to study the optimization of amine loading on MS.

Amine and Olefin Complexes of Pt(II) Having a PCP-Pincer Ligand

Park, Soon-Heum Korean Chemical Society 2002 Bulletin of the Korean Chemical Society Vol.23 No.1

$Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(OTf)\;(OTf=CF_3SO_3^-)$ readily reacts with various amines to afford cationic amine complexes $[Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(amine)](OTf)\;(amine=NH_3,\;NHMe_2,\;NHC_4H_8,\;NH_2Ph,\;NH_2(Tol-p))$ in high yields. These complexes have been fully characterized by IR, $^1H-,\;^{19}F{^1H}-,\;and\;^{31}P{^1H}-NMR$ spectroscopy, and elemental analyses. Reaction of $Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(OTf)$ with acrylonitrile quantitatively produced the ${\pi}$-olefinic complex $Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(CH_2=CHCN)](OTf)$ which is only stable in solution in the presence of acrylonitrile. Attempt at isolating this complex in the pure solid state was failed due to partial decomposition into $Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(OTf)$ The equilibrium constants $(K_{eq}=[Pt(PCP)-(NH_2R)^+][CH_2=CHCN]/[Pt(PCP)(CH_2=CHCN)^+][NH_2R]:\;[Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(CH_2=CHCN)]^++NH_2R{\rightleftarrows}[Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(NH_2R)]^++CH_2=CHCN=Ph,\;p-tolyl)$ were calculated to be 0.28 (for R = Ph) and 3.1 (R = p-tolyl) at $21^{\circ}C$. The relative stability of the ${\sigma}$-donor amine versus the ${\pi}$-olefinic acrylonitrile complex has been found largely dependent upon the amine-basicity $(pK_b)$, implicating that acrylonitrile practically competes with amine in the platinum coordination sphere. On the contrary to the formation of the acrylonitrile complex, no reaction of $Pt(2,6-(Cy_2PCH_2)_2C_6H_3)(OTf)$ with other olefins such as ethylene, styrene and methyl acrylate was observed.

Activity Improvements of an Engineered ω-transaminase for Ketones Are Positively Correlated with Those for Cognate Amines

Sang-Woo Han,Jong-Shik Shin 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.1

Chiral amines are broadly used as essential building blocks of diverse pharmaceutical drugs. ω- Transaminase (ω-TA) is one of the promising enzymes for biocatalytic preparation of chiral amines owing to its capability for reductive amination of ketones. However, industrial application of ω-TA to asymmetric synthesis of chiral amines is often limited by marginal activities for ketones. Here, we explore whether activity improvements of ω-TA for ketones caused by active site engineering are correlated with those for cognate amines. We examined amino donor reactivities of 20 amines with ω-TA from Ochrobactrum anthropi (OATA) and its engineered variant carrying a W58L mutation (OATAW58L). Consistent with the previously observed activity increases for ketones, OATAW58L showed 3 to 79-fold activity improvements for amines. Docking simulations suggested that the activity improvements resulted from increased proximity of a bound amine to an internal aldimine owing to relocation of a small binding pocket. Activity comparison indicated that the W58L mutation induced activity increases for ketones better than it did those for amines. This result motivated us to carry out Pearson correlation analysis among the activity improvements for amines and ketones, revealing a strong positive correlation (r = 0.84). This suggests that active site engineering beneficial for activity of ω-TA toward a target amine is likely to evoke an activity improvement for its cognate ketone. Our finding is expected to provide a clue to design a robust high-throughput screening method required for creation of an engineered mutant displaying a better activity toward a target ketone.

아민 및 아미드형 트리포달리간드에 기초한 분자설계 및 합성

강동현 ( Dong Hyun Kang ),최규성 ( Kyu Seong Choi ) 경남대학교 기초과학연구소 2013 기초과학지 Vol.30 No.-

본 연구에서는 아민,아미드형 트리포달 리간드인 L¹ (tris (2-benzylaminoetyl) amine), L² (tris(4-phenyl-3-aza-oxobutyl)amine)를 합성하였다, 새로운 형태의 트리포달 리간드인 L³(tris〔3-aza-2-oxo-4(2-pyridyl)buthl〕amine)는 (bis carboxymethylamino) acetic acetate와 2-(aminomethyl)pytidine을 반응시켜 합성하였으며, 70%의 수득율을 얻었다. 구조의 확인은 LR, NMR, 그리고 Mass를 통하여 합성을 확인하였다. We emplyed L¹ (tris (2-benzylaminoetyl) amine), L² (tris(4-phenyl-3-aza-oxobutyl)amine), L³ (tris〔3-aza-2-oxo-4(2-pyridyl)buthl〕amine) as amide-type tripidal ligands and syntyesized, L³ as a new member of the tripodal system wsa synthesized by the reaction of (bis carboxymethylamino) acetic acetate and 2-(aminomethyl)pytidine, The yield of final step for L² ,L³ was 65% and70% respectively, Comparative NMR, IR and FAB-mass data are also discussed.