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Selected microRNA-192 mutant indicates association with several function genes in bovine cells
Chen Zi,Dexin Zeng,Jiyong Zhou,Jianjun Dai,Luyan Jiang,Feng Xue,Yuan Jiang,Baoguang Li 한국유전학회 2018 Genes & Genomics Vol.40 No.4
MicroRNAs are implicated in many cellular processes such as cell differentiation and development, tumorigenesis, and immune regulation. In this study, miR192 was detected using quantitative real-time polymerase chain reaction (qRT-PCR) when MDBK cells were exposed to Escherichia coli. Cells with malfunction of bta-miR-192 were established using transcription activator-like effector nuclease (TALEN) technology. Finally, bta-miR-192 mutant cells were screened for differentially expressed genes using RNA-sequencing (RNA-seq). The results showed that miR192 significantly decreased in cells exposed to E. coli F18ac and E. coli K88ac. The RNA-seq results showed that 1673 differentially expressed transcripts were identified; 890 genes were upregulated and 775 genes were downregulated. With the gene ontology enrichment analysis, 431 differentially expressed genes (DEGs) were classified into 937 gene ontology terms. The pathway enrichment analysis showed that 535 genes were involved in 254 pathway terms. Interestingly, most of these DEGs were associated with the pathways in cancers or infectious diseases. When the selected DEGs (n = 162) in these pathways were intersected with 120 differential transcripts, 11 DEGs were identified. Subsequently, several genes associated with regulation, cancers, or viral infections, such as LEF1, AXIN2, MX1, and FCGR2B, were identified among the DEGs using functional analysis. Furthermore, associations between bta-miR-192 and DEGs were detected by intersecting the bta-miR-192’s target genes with the DEGs, indicating that three genes including CBL, DICER1 and TRERF1 were involved in this relationship. These findings provided useful guidance for investigating the role played by bta-miR-192 in cellular functionality in bovine cells.
Zi-Shu Chen,Ning-Ning Han,Jian Hong Li,Hu Wan,Guo-Hua Huang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The real-time quantitative reverse transcription PCR (qRT-PCR) is an efficient and pervasive technique for analysis gene expression. Despite the fact that this technique has been extensively used to explore the gene functions, the stability of the reference genes still requires being validated at different experimental conditions. This research aims to confirm that the expression stability of seven candidates reference genes under different concentrations sterile hemolymph containing virus (2, 4, 6, 108-fold) infects the Spodoptera frugiperda cells line (Sf9). The appropriate reference genes as endogenous controls were determined through four computational algorithms (ΔCt, NormFinder, BestKeeper, and geNorm) and an integrated online software RefFinder. According to results of this study, TUB and ACT1 were proved to be the most stable reference genes in E2 fold and E4 fold respectively. PPI was the best reference gene in E6 and E8 fold, 28S and TUB were the most stable pair reference genes through all samples. These results can facilitate basic researches of the molecular mechanism of HvAV- 3h and also prove to be helpful for the standard RT-PCR method.
chen zi,김승현 한국체육철학회 2018 움직임의철학 : 한국체육철학회지 Vol.26 No.4
The purpose of this study is to explore the identity of dragon dance in the Yeongnam region. To that end, the Commission conducted research on collecting and analyzing literature related to dragon dance activities in the Yeongnam region. The results are as follows. We can see that the formation of dragon dance characteristics in the Yeongnam region was closely related to the geographical environment and the long-standing cultural inertia of the agricultural economy. Youngnam culture is practical, open, receptive and innovative. Dragon dance in the Yeongnam region was Gyorim, Namgyeong Village, Geumryong, Bojae Cross Dragon, Yongho Lake, Jungdang, Saryongmu and Pungsung. It is the realization of cultural identity that preserves one's own cultural system and shows the harmony between mental appearance and national emotion. Thus, all classical things are challenged by modern culture, so what has a thousand years of history also requires reform and innovation. Materials, work, beauty and fitness combined, work and ecology used in the manufacture of work should be carefully and appropriately reformed in all areas. The slogan of cultural traditions is not following the same pattern but to develop and continuously innovate traditional cultures. 이 연구의 목적은 중국 영남 지역 용무의 정체성을 연구하는 것이다. 이를 위하여 중국 영남 지역 용무에 관련된 문헌 자료를 수집하고 분석하는 문헌연구를 하였다. 그 결과는 다음과 같다. 중국 영남 지역의 용무 정체성은 지리적 환경과 농업 경제의 오랜 문화적 관성과 밀접한 관계를 통해서 형성된 실용적이고 개방적이며 또한 수용적이고 혁신적이다. 중국 영남 지역 용무는 교림 연화 화룡, 남계촌 곤지 금룡, 보저 횡계룡, 용호 용무, 중산 최룡무, 하당 사룡, 담강 인룡무, 풍순 포채 화룡 등이 있었다. 중국 영남 지역 용무는 자신의 문화 체계를 보존하면서 정신적인 모습과 민족적 정서의 일치를 보여주었다. 따라서 고전적인 것들은 모두 현대 문화의 도전을 받고 있기 때문에 천년의 역사를 가지고 있는 영남 지역 용무 또한 개혁과 혁신이 필요하다. 용무의 제작에 사용되는 재료, 용무, 아름다움 그리고 체력 단련의 결합, 용무와 생태 환경 등등 각 분야를 모두 중요시하고 적절하게 개혁해야 한다. 문화 전승을 제창하는 것은 그대로 답습하는 것이 아니라 전통문화를 진정으로 발전시켜서 끊임없이 혁신시키는 것이다.
Sliding mode control based on neural network for the vibration reduction of flexible structures
Zi-chen Deng,Yong-an Huang,Wen-cheng Li 국제구조공학회 2007 Structural Engineering and Mechanics, An Int'l Jou Vol.26 No.4
A discrete sliding mode control (SMC) method based on hybrid model of neural network and nominal model is proposed to reduce the vibration of flexible structures, which is a robust active controller developed by using a sliding manifold approach. Since the thick boundary layer will reduce the virtue of SMC, the multilayer feed-forward neural network is adopted to model the uncertainty part. The neural network is trained by Levenberg-Marquardt backpropagation. The design objective of the sliding mode surface is based on the quadratic optimal cost function. In course of running, the input signal of SMC come from the hybrid model of the nominal model and the neural network. The simulation shows that the proposed control scheme is very effective for large uncertainty systems.
Improvement of Lipid and Terpenoid Yield in Thraustochytrids Using Chemical Regulators: A Review
Zi-Lei Chen,Lin-Hui Yang,Ling-Ling Tong,Yue Wang,Meng-Zhen Liu,Dong-Sheng Guo 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.5
For the increasing demand for lipids and terpenoids in humans, biological fermentation has become an attractive choice due to the safety and sustainability. Thraustochytrids have been identified as promising producers of polyunsaturated fatty acids because of their high lipid content and simple fatty acid composition. In addition, thraustochytrids are also potential producers of terpenoids for their completed mevalonate pathway. Chemical regulators can be used to stimulate or inhibit metabolic pathways, which are equivalent to effects of overexpression and suppression approaches. The application of chemical regulators is potentially an easy and practical approach to improve the lipid and terpenoid yield in thraustochytrids, which has become a research focus. In this review, the mechanisms of chemical regulators promoting lipid and terpenoid biosynthesis in thraustochytrids are elucidated. Various chemical regulators which can directly enhance the biosynthesis of lipids and terpenoids are summarized depending on the type of chemicals. In addition, some chemical regulators are demonstrated to indirectly improve lipid and terpenoid yield by reducing the conversion of metabolites and reducing oxidative stress. Therefore, the addition of chemical regulators can be a useful alternative strategy for improving lipid and terpenoid accumulation in large-scale cultivation of thraustochytrids.
( Zi Zhong Tang ),( Hui Chen ),( Li Jiao Chen ),( San Liu ),( Xue Yi Han ),( Qi Wu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
The carbohydrate-binding module (CBM) is an important domain of most cellulases that plays a key role in the hydrolysis of cellulose. The neutral endoglucanase (EG1) gene was reconstructed. A redesigned endoglucanase, named EG2, was constructed with a CBM containing a linker from Corticium rolfsii (GenBank Accession No. D49448). The redesigned EG genes were expressed in Escherichia coli, and their characteristics are discussed. Results showed that the degradation of cellulose by EG2 was about double that by EG1. The specific activities of EG1 and EG2 were tested under optimal conditions, and EG2 had higher activity (169.1 ± 2.74 U/mg) toward CMC-Na than did EG1 (84.0 ± 1.98) in the process of cellulose degradation. The optimal pH and temperature, pH stability, and heat stability of EG1 and EG2 were similar. Results indicated that the CBM plays an essential role in the hydrolysis of cellulose. We can improve EG`s catalytic power by adding the CBM from Corticium rolfsii.