Circular RNA circ_0024037 suppresses high glucose-induced lens epithelial cell injury by targeting the miR-199a-5p/TP53INP1 axis

Zhou Liping,Zheng Yanhua,Xu Yue,Shen Pincheng 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Background Diabetic cataract is a common ocular complication of diabetes. Circular RNA (circRNA) can participate in a variety of regulatory processes of a variety of eye diseases, including diabetic cataract. Objective Nowadays, the biological mechanism underlying circ_0024037 during diabetic cataract is not completely understood. This study was designed to explore the biological role of circ_0024037 in high glucose (HG)-induced lens epithelial damage. Result Circ_0024037 and TP53INP1 were significantly up-regulated while miR-199a-5p was significantly down-regulated in the diabetic cataract tissues and HG-induced human lens epithelial cells (HLECs). Knockdown of circ_0024037 significantly promoted the HG-induced HLECs cell proliferation, inhibited apoptosis, decreased MDA level as well as increased GSH-PX level. The dual-luciferase reporter assay and RIP assay showed that circ_0024037 served as a sponge of miR-199a-5p and miR-199a-5p could directly target TP53INP1 in HLECs. Conclusion Circ_0024037 knockdown protected HLECs from the HG-induced dysfunction by regulating the miR-199a-5p/TP53INP1 pathway in diabetic cataract. Our findings provid novel insights into the pathogenesis of diabetic cataract. Background Diabetic cataract is a common ocular complication of diabetes. Circular RNA (circRNA) can participate in a variety of regulatory processes of a variety of eye diseases, including diabetic cataract. Objective Nowadays, the biological mechanism underlying circ_0024037 during diabetic cataract is not completely understood. This study was designed to explore the biological role of circ_0024037 in high glucose (HG)-induced lens epithelial damage. Result Circ_0024037 and TP53INP1 were significantly up-regulated while miR-199a-5p was significantly down-regulated in the diabetic cataract tissues and HG-induced human lens epithelial cells (HLECs). Knockdown of circ_0024037 significantly promoted the HG-induced HLECs cell proliferation, inhibited apoptosis, decreased MDA level as well as increased GSH-PX level. The dual-luciferase reporter assay and RIP assay showed that circ_0024037 served as a sponge of miR-199a-5p and miR-199a-5p could directly target TP53INP1 in HLECs. Conclusion Circ_0024037 knockdown protected HLECs from the HG-induced dysfunction by regulating the miR-199a-5p/TP53INP1 pathway in diabetic cataract. Our findings provid novel insights into the pathogenesis of diabetic cataract.

Effects of Nitrate Addition on Rumen Fermentation, Bacterial Biodiversity and Abundance

Liping Zhao,Qingxiang Meng,Liping Ren,Wei Liu,Xinzhuang Zhang,Yunlong Huo,Zhenming Zhou 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.10

This study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with Ion Torrent sequencing and real-time polymerase chain reaction. Three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. Nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0.07). The alpha (p>0.05) and beta biodiversityof ruminal bacteria were not affected by nitrate. Nitrate increased typical efficient cellulolytic bacteria species (Ruminococcus flavefaciens, Ruminococcus ablus, and Fibrobacter succinogenes) (p<0.01; p = 0.06; p = 0.02). Ruminobactr, Sphaerochaeta, CF231, and BF311 genus were increased by 1% nitrate. Campylobacter fetus, Selenomonas ruminantium, and Mannheimia succiniciproducens were core nitrate reducing bacteria in steers and their abundance increased linearly along with nitrate addition level (p<0.01; p = 0.02; p = 0.04). Potential nitrate reducers in the rumen, Campylobacter genus and Cyanobacteria phyla were significantly increased by nitrate (p<0.01; p = 0.01).To the best of our knowledge, this was the first detailed view of changes in ruminal microbiota by nitrate. This finding would provide useful information on nitrate utilization and nitrate reducer exploration in the rumen.

Stress-Release Law and Deformation Characteristics of Large-Span Tunnel Excavated with Semi Central Diaphragm Method

Shen Zhou,Liping Li,Zhimin An,Hongliang Liu,Guangyu Yang,Pengfei Zhou 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.6

The proportion of large-span tunnels in newly-built highway tunnels is getting higher and higher, revealing the stress release law and deformation characteristics of the tunnel at different excavation steps has important guiding significance for the rational design of the supporting structure parameters. In this paper, a typical section of the Laohushan Tunnel was selected to analyze the stress and deformation response of the large-span tunnel excavated with the semi central diaphragm method (SCDM), with the numerical simulation and field monitoring method. The study found that during the excavation of the large-span tunnel with the SCDM, the stress and deformation response characteristics are different from those ordinary tunnels. Influenced by the settlement of the vault, there was a significant outward expansion tendency, and a high degree of stress concentration in the sidewall. In particular, the supporting structure at the sidewall has a deformation characteristic of expanding outward and then converging inward. In this paper, the in-depth analysis of the stress release law and deformation characteristics of the excavation process of large-span tunnels can provide reference for the excavation and support parameter design of similar projects.

Effects of Nitrate Addition on Rumen Fermentation, Bacterial Biodiversity and Abundance

Zhao, Liping,Meng, Qingxiang,Ren, Liping,Liu, Wei,Zhang, Xinzhuang,Huo, Yunlong,Zhou, Zhenming Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.10

This study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with Ion Torrent sequencing and real-time polymerase chain reaction. Three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. Nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0.07). The alpha (p>0.05) and beta biodiversityof ruminal bacteria were not affected by nitrate. Nitrate increased typical efficient cellulolytic bacteria species (Ruminococcus flavefaciens, Ruminococcus ablus, and Fibrobacter succinogenes) (p<0.01; p = 0.06; p = 0.02). Ruminobactr, Sphaerochaeta, CF231, and BF311 genus were increased by 1% nitrate. Campylobacter fetus, Selenomonas ruminantium, and Mannheimia succiniciproducens were core nitrate reducing bacteria in steers and their abundance increased linearly along with nitrate addition level (p<0.01; p = 0.02; p = 0.04). Potential nitrate reducers in the rumen, Campylobacter genus and Cyanobacteria phyla were significantly increased by nitrate (p<0.01; p = 0.01).To the best of our knowledge, this was the first detailed view of changes in ruminal microbiota by nitrate. This finding would provide useful information on nitrate utilization and nitrate reducer exploration in the rumen.

Potent antibacterial and antibiofilm activities of TICbf-14, a peptide with increased stability against trypsin

Wang Liping,Liu Xiaoyun,Ye Xinyue,Zhou Chenyu,Zhao Wenxuan,Zhou Changlin,Ma Lingman 한국미생물학회 2022 The journal of microbiology Vol.60 No.1

The poor stability of peptides against trypsin largely limits their development as potential antibacterial agents. Here, to obtain a peptide with increased trypsin stability and potent antibacterial activity, TICbf-14 derived from the cationic peptide Cbf-14 was designed by the addition of disulfide-bridged hendecapeptide (CWTKSIPPKPC) loop. Subsequently, the trypsin stability and antimicrobial and antibiofilm activities of this peptide were evaluated. The possible mechanisms underlying its mode of action were also clarified. The results showed that TICbf-14 exhibited elevated trypsin inhibitory activity and effectively mitigated lung histopathological damage in bacteria-infected mice by reducing the bacterial counts, further inhibiting the systemic dissemination of bacteria and host inflammation. Additionally, TICbf-14 significantly repressed bacterial swimming motility and notably inhibited biofilm formation. Considering the mode of action, we observed that TICbf-14 exhibited a potent membrane-disruptive mechanism, which was attributable to its destructive effect on ionic bridges between divalent cations and LPS of the bacterial membrane. Overall, TICbf-14, a bifunctional peptide with both antimicrobial and trypsin inhibitory activity, is highly likely to become an ideal candidate for drug development against bacteria.

Delayed Anaphylaxis to Red Meat Associated With Specific IgE Antibodies to Galactose

Liping Wen,Junxiong Zhou,Jia Yin,Jin-lu Sun,Kai Wu,Rohit katial,Yi Sun 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.1

ducAnovel delayed anaphylactic reaction to red meat, associated with tick bites and IgE antibodies against galactose-α-1, 3-galactose (α-gal), was reported in 2009 in the US, Australia and Europe. In this case, serum specific IgE to galactose-α-1, 3-galactose (>100 kU/L) and IgE to multiple non-primate mammalian proteins were positive. However, the pathogenesis of this disease remains unclear. We report the first case in Asia of delayed anaphylactic reaction to red meat, which was induced by bites from the hard tick, Hematophagous ixodidae. We confirmed the increased concentration of IgE reactive epitopes in non-primate mammalian organs, which may be rich in α-gal proteins in lymphatic and endothelial tissues. All confirmed ticks associated with this disorder in the literature and in our case belonged to the hard tick family. We hypothesize that hard tick saliva is enriched with blood-type substances, such as oligosaccharides, from the non-primate mammal victim’s blood after days to weeks of blood sucking, which sensitizes humans through the injection route while blood sucking. ducA novel delayed anaphylactic reaction to red meat, associated with tick bites and IgE antibodies against galactose-α-1, 3-galactose (α-gal), was reported in 2009 in the US, Australia and Europe. In this case, serum specific IgE to galactose-α-1, 3-galactose (>100 kU/L) and IgE to multiple non-primate mammalian proteins were positive. However, the pathogenesis of this disease remains unclear. We report the first case in Asia of delayed anaphylactic reaction to red meat, which was induced by bites from the hard tick, Hematophagous ixodidae. We confirmed the increased concentration of IgE reactive epitopes in non-primate mammalian organs, which may be rich in α-gal proteins in lymphatic and endothelial tissues. All confirmed ticks associated with this disorder in the literature and in our case belonged to the hard tick family. We hypothesize that hard tick saliva is enriched with blood-type substances, such as oligosaccharides, from the non-primate mammal victim’s blood after days to weeks of blood sucking, which sensitizes humans through the injection route while blood sucking.

The SIMP-SRV Method for Stiffness Topology Optimization of Continuum Structures

Zhou, Xiangyang,Chen, Liping,Huang, Zhengdong Society for Computational Design and Engineering 2007 International Journal of CAD/CAM Vol.7 No.1

In density-based topology optimization, 0/1 solutions are sought. Discrete topological problems are often relaxed with continuous design variables so that they can be solved using continuous mathematical programming. Although the relaxed methods are practical, grey areas appear in the optimum topologies. SIMP (Solid Isotropic Microstructures with Penalization) employs penalty schemes to suppress the intermediate densities. SRV (the Sum of the Reciprocal Variables) drives the solution to a 0/1 layout with the SRV constraint. However, both methods cannot effectively remove all the grey areas. SRV has some numerical aspects. In this work, a new scheme SIMP-SRV is proposed by combining SIMP and SRV approaches, where SIMP is employed to generate an intermediate solution to initialize the design variables and SRV is then adopted to produce the final design. The new method turned out to be very effective in conjunction with the method of moving asymptotes (MMA) when using for the stiffness topology optimization of continuum structures for minimum compliance. The numerical examples show that the hybrid technique can effectively remove all grey areas and generate stiffer optimal designs characterized with a sharper boundary in contrast to SIMP and SRV.

Image Deblocking Scheme for JPEG Compressed Images Using an Adaptive-Weighted Bilateral Filter

( Liping Wang ),( Chengyou Wang ),( Wei Huang ),( Xiao Zhou ) 한국정보처리학회 2016 Journal of information processing systems Vol.12 No.4

Due to the block-based discrete cosine transform (BDCT), JPEG compressed images usually exhibit blocking artifacts. When the bit rates are very low, blocking artifacts will seriously affect the image`s visual quality. A bilateral filter has the features for edge-preserving when it smooths images, so we propose an adaptiveweighted bilateral filter based on the features. In this paper, an image-deblocking scheme using this kind of adaptive-weighted bilateral filter is proposed to remove and reduce blocking artifacts. Two parameters of the proposed adaptive-weighted bilateral filter are adaptive-weighted so that it can avoid over-blurring unsmooth regions while eliminating blocking artifacts in smooth regions. This is achieved in two aspects: by using local entropy to control the level of filtering of each single pixel point within the image, and by using an improved blind image quality assessment (BIQA) to control the strength of filtering different images whose blocking artifacts are different. It is proved by our experimental results that our proposed image-deblocking scheme provides good performance on eliminating blocking artifacts and can avoid the over-blurring of unsmooth regions.

Comparison of a Cation Exchange Membrane and a Ceramic Membrane in Electrosynthesis of Ammonium Persulfate by a Pilot Experimental Study

Zhou, Junbo,Wang, Chao,Guo, Yujing,Gao, Liping The Korean Electrochemical Society 2019 Journal of electrochemical science and technology Vol.10 No.2

In order to improve the current efficiency and reduce the energy consumption in the electrosynthesis of ammonium persulfate, electrolytic properties of a perfluorosulfonic cation exchange membrane named PGN membrane and the $Al_2O_3$ ceramic membrane in the electrosynthesis of ammonium persulfate were studied and compared in a pilot electrolytic cell using a welded platinum titanium as the anode and a Pb-Sb alloy as the cathode. The effect of cell voltage, electrolyte flow rate and electrolysis time of the electrolytes on the current efficiency and the energy consumption were studied. The results indicated that the PGN membrane could improve current efficiency to 95.12% and reduce energy consumption to $1110kWh\;t^{-1}$ (energy consumption per ton of the ammonium persulfate generated) under the optimal operating conditions and the highest current efficiency of the $Al_2O_3$ ceramic membrane was 72.61% with its lowest energy consumption of $1779kWh\;t^{-1}$. Among 5 times of the electrolysis of the electrolytes, the lowest current efficiency of the PGN membrane was 85.25% with the highest energy consumption of $1244kWh\;t^{-1}$ while the lowest current efficiency of the $Al_2O_3$ ceramic membrane was 67.44% with the highest energy consumption of $1915kWh\;t^{-1}$, which suggested the PGN membrane could be used in the 5-stage electrolytic cell for the industrially continuous electrosynthesis of ammonium persulfate. Therefore the PGN membrane can be efficient to improve the current efficiency and reduce the energy consumption and can be applied in the industrial electrosynthesis of ammonium persulfate.

The Effects of Image Dehazing Methods Using Dehazing Contrast-Enhancement Filters on Image Compression

( Liping Wang ),( Xiao Zhou ),( Chengyou Wang ),( Weizhi Li ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

To obtain well-dehazed images at the receiver while sustaining low bit rates in the transmission pipeline, this paper investigates the effects of image dehazing methods using dehazing contrast-enhancement filters on image compression for surveillance systems. At first, this paper proposes a novel image dehazing method by using a new method of calculating the transmission function―namely, the direct denoising method. Next, we deduce the dehazing effects of the direct denoising method and image dehazing method based on dark channel prior (DCP) on image compression in terms of ringing artifacts and blocking artifacts. It can be concluded that the direct denoising method performs better than the DCP method for decompressed (reconstructed) images. We also improve the direct denoising method to obtain more desirable dehazed images with higher contrast, using the saliency map as the guidance image to modify the transmission function. Finally, we adjust the parameters of dehazing contrast-enhancement filters to obtain a corresponding composite peak signal-to-noise ratio (CPSNR) and blind image quality assessment (BIQA) of the decompressed images. Experimental results show that different filters have different effects on image compression. Moreover, our proposed dehazing method can strike a balance between image dehazing and image compression.