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Zhili Li 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.2
This study was carried out to develop a carbamide-based dithiocarbamate (CDTC) chelator for theremoval of heavy metal ions from wastewater. Its structural properties were characterized by FT-IR, 1HNMR and 13C NMR. Results confirmed the functional groups of –HNC(55S)S– existed. The adsorptionisotherms showed CDTC had a high adsorption capability for Zn (119.8 mg/g) and Cu (63.1 mg/g). Itexhibited a distinctive selectivity for the removal of metal ions (Cu2+ > Zn2+ > Cr3+ > Pb2+ > Cd2+) asthey coexisted. The influence of initial pH of wastewater for the removal efficiency of metal ions was alsoinvestigated and a pH > 7 was preferred.
Adsorption of different molecular weight lignosulfonates on dimethomorph powder in an aqueous system
Zhili Li,Yuxia Pang,Yuanyuan Ge,Xueqing Qiu 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.1
Different molecular weight lignosulfonates were acquired by ultrafiltration and their adsorption behavior on dimethomorph powder were investigated. The results showed that as the molecular weight of lignosulfonates increased the adsorption amounts increased and the adsorption processes were driven by van der Waals forces and hydrophobic effects. The adsorption isotherms were fitted well by Langmuir model. Relations of adsorption amounts and zeta potentials with particle size of dimethomorph particles were established to disclose the dispersing mechanism of dimethomorph suspensions. The average particle sizes were determined by zeta potentials rather than adsorption amounts, which confirmed the dispersing mechanisms were electrostatic repulsive forces.
Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
Li, Han,Wang, Xiangming,Wu, Yan,Zhang, Dingran,Xu, Hongyang,Xu, Hongrun,Xing, Xiaoguang,Qi, Zhili Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9
Objective: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. Methods: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. Results: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). Conclusion: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.
Association of Apolipoprotein E Polymorphisms with White Matter Lesions and Brain Atrophy
ZhiLi Niu,PingAn Zhang,Dong Li,ChengLiang Zhu,LiNa Feng,Ge Xiong,NaNa Song,Pei Tang,Feng Liu 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.2
Objective Apolipoprotein E (ApoE) is mainly synthesized in the liver. So far, it is unknown the relationship among APOE gene polymorphisms and WML, brain atrophy. Therefore, the aim of the study was to assess the associations of APOE gene polymorphisms in patients with WML and brain atrophy. Methods A total of 58 patients with WML, 128 patients with brain atrophy, 112 patients with co-occurrence of WML and brain atrophy and 95 healthy elderly volunteers were recruited from Renmin Hospital of WuHan University. Results Allele E3 was the most common allele. The alleles E2 had significantly higher levels of ApoB and lower age in WML group. The alleles E2 was associated with the lower level of ApoB, LDL-Ch, TCh, and sdLDL in co-occurrence group. The E3/E3 genotype has higher level of sdLDL, but lower age and female frequency in WML. The E3/E4 genotype had higher level of TG, but lower age in WML. Gender, Age, E2, Hyperhomocysteinemia and UA were also significantly associated with disease progression. Conclusion This study found that clinical data, lipids and metabolic complications were closely related to ApoE genotypes and alleles, and also disease progression and type.
MicroRNA expression profiles in conventional and micropropagated Dendrobium officinale
Zhili Yang,Dongfeng Yang,Xianfeng Ding,Yong Gao,Donghai Li,Tao Xu 한국유전학회 2015 Genes & Genomics Vol.37 No.4
MicroRNA (miRNA) is defined as an endogenous *22 nt noncoding RNA that has critical gene-regulatory functions in plants. Dendrobium officinale is among the most important herbs in traditional Chinese medicine. However, no miRNA of D. officinale has been reported to date. To identify the miRNA in D. officinale and to detect the differentially expressed miRNA between conventional and micropropagated D. officinale plants, miRNA microarray was performed following quantitative real-time RTPCR validation. A total of 120 miRNAs from 37 miRNA families were identified in both conventional and micropropagated D. officinale plants. Among the total number of miRNAs, 45 miRNAs from six families were differentially expressed, including five up-regulated families (miR156, miR164, miR171, miR827 and miR529) and one downregulated family (miR167) in micropropagated group. Web microrna designer and gene ontology term-enrichment analyses were applied for prediction and functional analysis of the target genes of differentially expressed miRNA. Such miRNAs are mainly related to hormone stimulus response, biological and metabolic process regulation, formation of intracellular organelle, and nucleic acid binding.
Insight into Structural Aspects of Histidine 284 of Daphnia magna Arginine Kinase
Rao, Zhili,Kim, So Young,Li, Xiaotong,Kim, Da Som,Kim, Yong Ju,Park, Jung Hee Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.9
Arginine kinase (AK), a bioenergy-related enzyme, is distributed widely in invertebrates. The role of highly conserved histidines in AKs is still unascertained. In this study, the highly conserved histidine 284 (H284) in AK of Daphnia magna (DmAK) was replaced with alanine to elucidate the role of H284. We examined the alteration of catalytic activity and structural changes of H284A in DmAK. The catalytic activity of H284A was reduced dramatically compared to that in wild type (WT). Thus the crystal structure of H284A displayed several structural changes, including the alteration of D324, a hydrogen-bonding network around H284, and the disruption of π-stacking between the imidazole group of the H284 residue and the adenine ring of ATP. These findings suggest that such alterations might affect a conformational change of the specific loop consisting of G310-V322 at the antiparallel β-sheet region. Thus, we speculated that the H284 residue might play an important role in the conformational change of the specific loop when ATP binds to the substrate-binding site of DmAK.
Lei Wang,Zhili Wu,Linyan Li,Weizhong Liu,Yong Hu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2
Escherichia coli MinE is required for placement of a division septum. A nucleus-encoded and plastidtargeted MinE homologue, CrMinE, was identified from Chlamydomonas reinhardtii. Similar to the Arabidopsis nucleus-encoded AtMinE, the CrMinE protein possesses extra N-terminal and C-terminal extensions relative to the eubacterial and other algal plastid-encoded MinE proteins. The CrMinE protein functions in plastids, as revealed by a transient expression assay using a full-length CrMinE protein fused to enhanced green fluorescent protein. In addition, the overexpressed CrMinE:EGFP in wild-type E. coli with clear coiled structures could still recognize the cell division site of the host cell, which suggests evolutionary conservation of the MinE mode of action. No MinE homologue was found in a search of all researched plastid genome sequences of land plants; moreover, the researched MinEs of land plants are encoded by nuclear genomes. Thus, the identification of the CrMinE located in the nuclear genome implies that the transfer events of MinE from plastid to nucleus might have occurred before the evolution of land plants and during the evolution of green algae.
Sulfonated multi-walled carbon nanotubes for the removal of copper (II) from aqueous solutions
Yuanyuan Ge,Zhili Li,Duo Xiao,Piao Xiong,Na Ye 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4
Sulfonated multi-walled carbon nanotubes (s-MWCNTs) was prepared from purified multi-walled carbon nanotubes (p-MWCNTs) by concentrated H2SO4 at elevated temperature. The structure was characterized by SEM, FTIR, Raman, XPS, and BET. It could be dispersed steadily in water at a dosage of 1.0 mg/mL for a week. The adsorption performance of s-MWCNTs toward Cu(II) was investigated including the effects of pH and ionic strength. Results indicated the adsorption was much dependent on pH but not on ionic strength. The adsorption capacity for Cu(II) was enhanced 58.9% via the sulfonation. Moreover, the adsorption mechanisms were carefully analyzed by Freundlich and D-R models.
Yuanyuan Ge,Zhili Li,Yan Kong,Quanpeng Song,Kunqi Wang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6
Bi-functionalized lignin with amino and sulfonic groups (ASL) was synthesized via Mannich reaction andsulfomethylation. It was systematically characterized by FT-IR, element analysis, surface charge and XPS. Effects of initial pH, contact time and initial metal ion concentration on the adsorption of Cu(II) and Pb(II)onto ALS were studied. Results indicated that the biosorbent showed excellent performance for metalseven from low pH solutions. The adsorption kinetics and isotherms could be described well with Pseudosecond-order and D–R model, respectively. Further investigation of the metal-loaded ASL by FT-IR andXPS elucidated the amino and sulfonic groups reacted with metals in different way.