Srap Molecular Marker in the Round Shape Trait of Cucumber Fruit

Dan-dan Chai,Zhi-hui Cheng,Huan-wen Meng,Shu-xia Chen,Ya-li Li 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Structural optimization of rapidly separating microneedles for efficient drug delivery

Dan Dan Zhu,Bo Zhi Chen,Meng Chan He,Xin Dong Guo 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.51 No.-

Rapidly separating microneedles (RSMNs) arose as an improvement of traditional MNs for the specialseparable structures. The aim of this study was to investigate various structural parameters contributedto the delivery performance of RSMNs. The experimental results indicated that the optimized RSMNswith 500 mm-long solid PLA MNs, 250 mm-long overlap and 500 mm-long dissolving MNs delivered over95% of drugs within 30 s. In the in vivo diabetic mice treatment, insulin loaded optimized RSMNs reachedapproximately the same therapeutic effect on lowering the glucose in blood as injection, significantlybetter than traditional MNs.

Immunization of BALB/c mice with Brucella abortus 2308DwbkA confers protection against wild-type infection

Zhi-qiang Li,Dan Gui,Zhi-hua Sun,Jun-bo Zhang,Wen-zhi Zhang,Hui Zhang,Fei Guo,Chuangfu Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.4

Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DwbkA) and evaluated its virulence. The survival of 2308DwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.

The Phytotoxic Effects of Selenium–Mercury Interactions on Root Growth in Brassica rapa (LvLing)

Zhi-Wei Bian,Jian Chen,Hui Li,Dan-Dan Liu,Li-Fei Yang,Yue-Lin Zhu,Wen-Li Zhu,Wei Liu,Zheng-Zheng Ying 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.3

Rapid industrial and agricultural development has dramatically increased the emission of selenium (Se) and mercury (Hg) into the environment. Combined soil pollution by Se and Hg poses a potential threat to crop production. However, no toxic effects of Hg–Se interactions on plants have been reported previously. In this study, we investigated the effects of Hg–Se interactions on biochemical and physiological indices in the roots of Brassica rapa (LvLing). Seedlings were treated hydroponically with solutions of mercury chloride (1 μM), sodium selenite (4 μM), or a combination of the two. Combined Hg+Se treatment significantly inhibited root growth, reduced root biomass, and enhanced reactive oxygen species (ROS) and malondialdehyde accumulation and led to a loss of plasma membrane integrity. The combined treatment increased glutathione peroxidase, glutathione S-transferase, and peroxidase activity, reduced superoxide dismutase activity, and had no effect on catalase activity. In addition, we detected increased glutathione concentrations in root tips and reduced ascorbic acid concentrations in the presence of Hg+Se relative to individual treatments with these elements. Thus, Hg–Se interactions enhanced oxidative injury, cell death, and phytotoxicity in B. rapa roots.

Immunotherapeutic Effects of Dendritic Cells Pulsed with a Coden-optimized HPV 16 E6 and E7 Fusion Gene in Vivo and in Vitro

Zhou, Zhi-Xiang,Li, Dan,Guan, Shan-Shan,Zhao, Chen,Li, Ze-Lin,Zeng, Yi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Cervical cancer is the second most common cause of cancer related death of women. Persistent HPV infection, especially with high-risk types such as HPV16 and HPV18, has been identified to be the primary cause of cervical cancer. E6 and E7 are the major oncoproteins of high-risk HPVs, which are expressed exclusively in HPV infected tissues, and thereby represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this work, we used recombinant adenovirus expressing coden-optimized HPV16 E6 and E7 fusion protein (Ad-ofE6E7) to prime dendritic cells (DC-ofE6E7), to investigate the ability of primed DC vaccine in eliciting antitumor immunity in vitro and vivo. Results: Our results indicated that DC-ofE6E7 vaccine co-culturing with splenocytes could strongly induce a tumor-specific cytotoxic T lymphocyte (CTL) response and kill the TC-1 cells effectively in vitro. Moreover, DC-ofE6E7 vaccine induced protective immunity against the challenge of TC-1 cancer cells in vivo. Conclusions: The results suggested that the HPV16 ofE6E7 primed DC vaccine has potential application for cervical cancer immunotherapy.

Electrokinetic techniques, their enhancement techniques and composite techniques with other processes for persistent organic pollutants remediation in soil: A review

Yuxin Chen,Dan Zhi,Yaoyu Zhou,Anqi Huang,Shikang Wu,Bin Yao,Yifei Tang,Chaoran Sun 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

Persistent organic pollutants (POPs) in soil have caused widespread concern, which is necessary to developefficientand greentechnologies.Electrokinetic (EK)remediationtechnology,theirenhancementtechniquesand composite techniques with other processes have shown broad application prospects in thefield of soilPOPs remediation. In this paper, the progressof EK remediation technology in the remediation of POPs in soilin recent years is reviewed. It can be seen that some technologies need to be further studied and evaluated,and it is inappropriate to simplycompare them.The effectoffield remediation is boundto be affected byfieldconditions,andthefinal datawill be differentfromthelaboratory data. Some emerging technologies,such asadvanced oxidation technology and nanotechnology, demonstrated high POPs removal rates when coupledwith EK remediation technology. This paper provides some insights into the future development of thetechnology and provides reference for the selection of technology in practical application.

Self-Supported Bi2MoO6 Nanosheet Arrays as Advanced Integrated Electrodes for Li-Ion Batteries with Super High Capacity and Long Cycle Life

Weibin Chen,Lina Zhang,Zhi-jing Ji,Ya-dan Zheng,Shuang Yuan,QIANG WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.6

Hierarchical Bi2MoO6 nanosheet arrays (BNAs) growing on three-dimensional (3D) Ni foam are synthesized by one-step template-free route. The obtained BNAs are used as binder-free integrated electrode for Li-ion batteries (LIBs) directly. The electrode exhibits a super high reversible discharge capacity of 2311.7 μAh·cm-2 (1741.4 mAhg-1), and an excellent cycle stability. The outstanding electrochemical properties are reasonable from the self-supported integrated electrode in which the electrolyte is easy to infiltrate active materials, electrons and ions are readily transported along the 3D conductive substrate and the stable electrode structure.

Clinical experience of US-guided HIFU Treatment for Uterine Fibroid &Adenomyosis

당량담 ( Liang Dan Tang ),( Wen Zhi Chen ),( Lian Zhang ),( Zhi Biao Wang,) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

Patterns of Lymph Node Recurrence after Radical Surgery Impacting on Survival of Patients with pT1-3N0M0 Thoracic Esophageal Squamous Cell Carcinoma

Xiao-li Chen,,Tian-wu Chen,Zhi-jia Fang,Xiao-ming Zhang,Zhen-lin Li,Hang Li,Hong-jie Tang,Li Zhou,Dan Wang,Zishu Zhang 대한의학회 2014 Journal of Korean medical science Vol.29 No.2

The aim of this study was to investigate how patterns of lymph nodes recurrence after radical surgery impact on survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma. One hundred eighty consecutive patients with thoracic esophageal squamous cell carcinoma underwent radical surgery, and the tumors were staged as pT1-3N0M0 by postoperative pathology. Lymph nodes recurrence was detected with computed tomography 3-120 months after the treatment. The patterns of lymph nodes recurrence including stations, fields and locations of recurrent lymph nodes, and impacts on patterns of survival were statistically analyzed. There was a decreasing trend of overall survival with increasing stations or fields of postoperative lymph nodes involved (all P<0.05). Univariate analysis showed that stations or fields of lymph nodes recurrence, and abdominal or cervical lymph nodes involved were prognostic factors for survival(all P<0.05). Cox analyses revealed that the field was an independent factor (P<0.05, odds ratio=2.73). Lymph nodes involved occurred predominantly in cervix and upper mediastinum (P<0.05). In conclusion, patterns of lymph node recurrence especially the fields of lymph nodes involved are significant prognostic factors for survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma.

A DFT study of the geometrical structures and electronic properties of CumMn (m + n = 6, M = Fe, Co, Ni) clusters

Wang Su Juan,Zhou Dan,Hu Yan Fei,Tang Cui Ming,Wang Qiao Zhi,Chen Shun Ling 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.3

In this work, we investigated bimetallic CumMn (m + n = 6, M = Fe, Co, Ni) clusters on the basis of the density functional theory at the M06L level. While changing the structure and composition, our team studied the geometrical structures, relative stabilities and electronic magnetic moments of these clusters. We found that the average atomic binding energies vary linearly with the number of doping atoms in the CumFen and the CumNin clusters. The values of electron affinity (EA) and the ionization potential (IP) for the CumNin clusters are closer to those of pure Cu clusters compared with the others. A negative value of the EA is only seen in CumFen clusters. The electronic magnetic moments of these clusters indicate that most of them exhibit a local minimum when doped with three atoms. Moreover, we further analyzed the HOMO–LUMO gaps, the natural population, and the geometrical structures.