Physicochemical Characterization and an Injection Formulation Study of Water Insoluble ZCVI4-2, a Novel NO-Donor Anticancer Compound

Yuan Gao,Jianjun Zhang,Li Li,Feng Su,Zhenhua Gong,Yisheng Lai,Yihua Zhang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.7

ZCVI4-2 was a novel nitric oxide-releasing glycosyl derivative of oleanolic acid that displayed strong cytotoxicity selectively against human hepatocellular carcinoma in vitro and in vivo. In this study, ZCVI4-2 was characterized by FT-IR spectroscopy, differential scanning calorimetry,powder X-ray diffractometry, Raman spectroscopy, hygroscopicity and stability. A high performance liquid chromatography method was also established for the quantitative determination of solubility and additional stability profile of ZCVI4-2. ZCVI4-2 was found to be an amorphous and stable solid with low solubility of less than 10 μg/mL. Based on the solubilization tests that included methods of cosolvency and micellization, the solution mixture of 5% Solutol HS-15, 5% 1, 2-propylene glycol and 5% anhydrous ethanol was determined to be the system for the preparation of the ZCVI4-2 early injection solution. The effect of pH, temperature, light and injectable isotonic glucose or NaCl solution on ZCVI4-2 injection was also investigated. Good stability was observed at all testing conditions. Under the conditions studied, the NO-releasing rate and amount of ZCVI4-2 from the early injection solution in rat plasma demonstrated a promising therapeutic efficacy while maintaining a good safety profile.

First-principles study of Ti doping in FeF3$0.33H2O

Zhenhua Yang,Zhijuan Zhang,Yalong Yuan,Yunqing Huang,Xianyou Wang,Xiaoying Chen,Shuangying Wei 한국물리학회 2016 Current Applied Physics Vol.16 No.8

The effect of Ti doping on the geometrical and electronic structures of FeF3$0.33H2O are systematically investigated by using the first principles calculations. We focused on TixFe1-xF3$0.33H2O systems, in which x is equal to 0, 0.08, 0.17 and 0.25, respectively. Different kinds of Ti dopant sites are checked and the most stable structure can be obtained by comparison of total energy. The crystal volume of TixFe1- xF3$0.33H2O expands gradually with increasing Ti doping concentration. Calculated formation energies indicate TixFe1-xF3 is easiest to fabricate and the difficulty of Ti doping FeF3 with hexagonal-tungstenbronze( HTB) structure decreases with the increase of Ti doping concentration under the Fe-rich and Ti-rich growth conditions. Moreover, TixFe1-xF3$0.33H2O is thermodynamically stable, indicating that water molecule can preferentially occupy one-dimensional cavity in the TixFe1-xF3. The band gap of TixFe1-xF3$0.33H2O decreases with increasing Ti doping concentration and Ti0.25Fe0.75F3$0.33H2O exhibits character of half metal, indicating that the conductivity of FeF3$0.33H2O can be improved by Ti-doping. Besides, it can be confirmed that Ti-doping also can broaden the hexagonal cavity in the FeF3$0.33H2O by analyzing the crystal structure of FeF3$0.33H2O and TixFe1-xF3$0.33H2O. With excellent conductivity and larger hexagonal cavity, TixFe1-xF3$0.33H2O can afford open diffusion channels. Therefore, Li ions can remain unblocked, which is beneficial to fast charge and discharge.

Whole RNA-sequencing and gene expression analysis of Trichoderma harzianum Tr-92 under chlamydospore-producing condition

Min Yuan,Yuanyuan Huang,Zhenhua Jia,Weina Ge,Lan Zhang,Qian Zhao,Shuishan Song,Yali Huang 한국유전학회 2019 Genes & Genomics Vol.41 No.6

Background Trichoderma is one of the most important biocontrol fungi, which could produce mycelia, conidiospores, and chlamydospores three types of propagules under different conditions. Chlamydospores are produced in harsh conditions in various fungi, and may be more resistant to adverse conditions. However, the knowledge associated with the mechanism of chlamydospore formation remained unclear in Trichoderma. Objectives This study is aimed to explore the essential genes and regulatory pathways associated with chlamydospore formation in Trichoderma. Methods The culture condition, survival rate, and biocontrol effects of chlamydospores and conidiospores from Trichoderma. harzianum Tr-92 were determined. Furthermore, the whole transcriptome profiles of T. harzianum Tr-92 under chlamydospore-producing and chlamydospore-nonproducing conditions were performed. Results T. harzianum Tr-92 produced chlamydospores under particular conditions, and chlamydospore-based formulation of T. harzianum Tr-92 exhibited higher biocontrol ability against Botrytis cinerea in cucumber than conidoiospore-based formulation. In the transcriptome analysis, a total of 2,029 differentially expressed genes (DEGs) were identified in T. harzianum Tr-92 under chlamydospore-producing condition, compared to that under chlamydospore-nonproducing condition. GO classification indicated that the DEGs were significantly enriched in 284 terms among biological process, cellular components and molecular function categories. A total of 19 pathways were observed with DEGs by KEGG analysis. Furthermore, fifteen DEGs were verified by quantitative real-time PCR, and the expression profiles were consistent with the transcriptome data. Conclusion The results would provide a basis on the molecular mechanisms underlying Trichoderma sporulation, which would assist the development and application of fungal biocontrol agents.

Effect of Anhydride Grafting Agent on Trap Levels of Low-Density Polyethylene

Lijuan He,Zhenhua Yuan,Xiong Yang,Huiqing Niu,Chao Zhu,Dawei Li,Lei Zhao,Chuntian Chen 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.5

Trap distribution of the low-density polyethylene(LDPE) modified by anhydride grafting agent was studied and the effect of grafting agent on the trap levels was analyzed by the method of Photo-stimulated Discharge in this paper. Different concentrations and types of same concentration of LDPE modified by anhydride grafting agent were measured by using continuous UV scanning. The results showed that different concentrations and types of grafting agent had effect on trap levels distribution of LDPE. The trap depth would decrease with the anhydride grafting agent content increasing and increase with its damaged condition increasing when anhydride grafting agent was destroyed. Anhydride grafting agent are widely applied to the production and research of high pressure insulating polyethylene materials. The purpose of this paper is to elaborate the effect of anhydride grafting agent on the insulating properties of polyethylene material and explain the strongest binding capacity of anhydride grafting agent to space charge.

Evaluation of Fungal Lactic Acid Accumulation Using Glycerol as the Sole Carbon Source

Xiaoqing Wang,Zhenhua Ruan,Webster Guan,Robert Kraemer,Yuan Zhong,Yan Liu 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3

This study investigated the glycerol utilization for lactic acid accumulation by the filamentous fungus Rhizopus oryzae 9363 and compared it with the conventional carbon source glucose. No lactic acid accumulated in the glycerol media at 30oC, in contrast to the glucose media. Increasing the temperature from 30 to 37oC led to a 63% decrease in the average growth rate of R. oryzae in glycerol media and a 61% increase in the average cell mass yield, and the cultures on glycerol media at 37oC were able to generate 0.6 g/L lactic acid. While, raising temperature significantly inhibited lactic acid production on glucose media. Moreover, supplementing cultures with sodium pyruvate significantly improved the lactic acid synthesis of R. oryzae on glycerol media, with lactic acid concentrations reaching 1.33 g/L at 37oC and 0.67 g/L at 30oC, respectively. Our results indicate that glycerol utilization for lactic acid accumulation by Rhizopus sp. is limited by the availability of intracellular pyruvate, and controlling pyruvate flow is a key to enhancing the lactic acid accumulation.

Thermo-mechanical analysis of angular contact ball bearing

Wei Bian,Zhenhua Wang,Juntang Yuan,Weiwei Xu 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.1

The thermal-mechanical character, which is difficult to ensure because of the lack of a corresponding theory and tool, has a significanteffect on the dynamics of bearings. It even leads to a sudden failure of bearings in a working situation. In this research, a thermal deformationmodel was established, based on the analysis of temperature effect on the basic size of angular contact ball bearing. And thetransmission from rolling size to bearing axial stiffness was explicit. On the basis of the variation of Hertz contact stiffness and thechange of initial contact angle of angular contact ball bearing caused by temperature rise, a “Thermo- mechanical” model of bearing wasproposed. According to this model, using the corresponding calculation procedure programmed by MATLAB, the effect of bearing temperatureon the axial stiffness has been studied. And the correctness of this model was verified with experiments. Some design suggestionshave been made for the decision of bearing preload: to prevent the bearing failure caused by overheating.

Energy efficiency optimization of water pump based on heuristic algorithm and computational fluid dynamics

Wang Wenjie,Han Zhenhua,Pei Jin,Pavesi Giorgio,Gong Xiaobo,Yuan Shouqi 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.1

To reduce the energy consumption of large centrifugal pumps, modified heuristic intelligent algorithms are used to directly optimize the diffuser of centrifugal pumps. Considering the hydraulic efficiency under the design condition as the optimization target, in this study, 14 geometric parameters such as the inlet diameter, outlet diameter, and leading and trailing vane angles of the diffuser are selected as design variables, and the modified particle swam optimization and gravitational search algorithm are used to directly search for optimization in the design space. The performance and loss of internal entropy production of the different models before and after optimization are compared and analyzed in detail. The results show that the global optimization ability of the modified algorithm is improved. The diffuser model changes from cylindrical to twisted, the vane wrap angle increases, and the thickness of the leading edge decreases. Under the design condition, the efficiency of modified particle swarm optimization algorithm solution is increased by 2.75% and modified gravitational search algorithm solution by 2.21%, while the power remains unchanged. Furthermore, the optimization solution has the largest lift efficiency improvement under part-load conditions. After optimization, the unstable flow in the model is improved and internal entropy production loss is reduced significantly. The interior of the diffuser is dominated by turbulent entropy production and direct entropy production under different operating conditions, and the wall entropy production accounts for the smallest proportion.

Inhibition of lncRNA KCNQ1OT1 Improves Apoptosis and Chemotherapy Drug Response in Small Cell Lung Cancer by TGF-β1 Mediated Epithelial-to-Mesenchymal Transition

Deyu Li,Qin Tong,Yuane Lian,Zhizhong Chen,Yaru Zhu,Weimei Huang,Yang Wen,Qiongyao Wang,Shumei Liang,Man Li,Jianjing Zheng,Zhenhua Liu,Huanxin Liu,Linlang Guo 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. Materials and Methods In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. Results The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. Conclusion Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.

SymWorkManager: A Novel HPC Multi-thread Programming Model

Xiaohui WEI,Hongliang LI,Shaocheng XING,Ji LI,Zane Zhenhua HU,Shutao YUAN 보안공학연구지원센터(IJGDC) 2009 International Journal of Grid and Distributed Comp Vol.2 No.1