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        Thermo-mechanical analysis of angular contact ball bearing

        Wei Bian,Zhenhua Wang,Juntang Yuan,Weiwei Xu 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.1

        The thermal-mechanical character, which is difficult to ensure because of the lack of a corresponding theory and tool, has a significanteffect on the dynamics of bearings. It even leads to a sudden failure of bearings in a working situation. In this research, a thermal deformationmodel was established, based on the analysis of temperature effect on the basic size of angular contact ball bearing. And thetransmission from rolling size to bearing axial stiffness was explicit. On the basis of the variation of Hertz contact stiffness and thechange of initial contact angle of angular contact ball bearing caused by temperature rise, a “Thermo- mechanical” model of bearing wasproposed. According to this model, using the corresponding calculation procedure programmed by MATLAB, the effect of bearing temperatureon the axial stiffness has been studied. And the correctness of this model was verified with experiments. Some design suggestionshave been made for the decision of bearing preload: to prevent the bearing failure caused by overheating.

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        Molecular cloning and characterization of a novel saltspecific responsive WRKY transcription factor gene IlWRKY2 from the halophyte Iris lactea var. chinensis

        Jun Tang,Qingquan Liu,Haiyan Yuan,Yongxia Zhang,Weilin Wang,Suzhen Huang 한국유전학회 2018 Genes & Genomics Vol.40 No.8

        Iris lactea var. chinensis is a perennial herbaceous halophyte with high salt tolerance and ornamental value. Previous RNA sequencing analysis revealed a transcription factor gene IlWRKY2 expression was upregulated by salt stress. To obtain the full-length sequence, the basic characteristics of IlWRKY2 and its expression pattern under salt stress. Full-length cDNA of IlWRKY2 was cloned by 3′/5′ RACE based on the intermediate sequence obtained by RNA sequencing analysis. Structure analysis of IlWRKY2 were performed by Compute pI/MW tool, PSIPRED and SWISS-MODEL analysis. Sequence analysis of IlWRKY2 were performed by BLAST program, DNAman software, MEGA software and MEME program. IlWRKY2 expression pattern was analyzed by quantitative real-time polymerase chain reaction. The open reading frame of IlWRKY2 is 1338 bp in length, which encodes a protein of 446 amino acids. Amino acid sequence analysis revealed that the IlWRKY2 contains one WRKY domains with a zinc finger motif C–X5–C–X23–H–X–H. Phylogenetic analysis showed that the IlWRKY2 was much closer to EgWRKY41 from Elaeis guineensis and MaWRKY42 from Musa acuminata subsp. malaccensis. Furthermore, the expression of IlWRKY2 in I. lactea var. chinensis shoots was upregulated by different concentrations of NaCl treatment and increased 16-fold after treatment with 200 mM NaCl for 12 h. Obtained the full-length cDNA of IlWRKY2 which belongs to Group II-b WRKY subfamily. IlWRKY2 expression was obviously induced by salt stress in I. lactea var. chinensis shoots and it may play an important role in halophyte I. lactea var. chinensis adaptation to environmental salt stress.

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