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Weifeng Gong,Xiaobo Guo,Yangmin Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.10
Purpose: Hepatitis C virus (HCV) poses a risk of chronic liver disease and threatens a significant number of people worldwide. MicroRNAs (miRNAs) are linked to the regulation of hepatocarcinogenesis. Although miR-373 is required for HCV infection, the underlying mechanisms of miR-373 involvement in HCV replication remain elusive. Materials and Methods: Quantitative reverse transcription PCR assays were performed to detect the abundances of miR-373 and HCV RNA either in Huh 7.5 cells or liver biopsy specimens with HCV infection. Luciferase assay was employed to probe the interactionsbetween miR-373 and interferon regulatory factor 5 (IRF5). Western blot was conducted to investigate the effect of miR-373 and IRF5 on HCV replication and activation of type 1 interferon (IFN) response in JFH1-infected Huh 7.5 cells. Results: HCV infection appeared to be caused by increased miR-373 expression. Addition of miR-373 promoted HCV RNA expression,while miR-373 depletion led to an inhibitive effect on HCV replication. Concordantly, IRF5, as a direct target, was limitedby miR-373 in JFH1-infected Huh 7.5 cells. In addition, introduction of IRF5 protected HCV replication in the presence of abundant miR-373. Furthermore, the miR-373-mediated inhibitory effect on type 1 IFN response was ablated following IRF5 accumulation. Conclusion: miR-373 abrogation reduced HCV replication via activation of type 1 IFN responses by targeting IRF5 in JFH1-infectedHuh 7.5 cells, suggesting a promising therapeutic for treating HCV infection.
Wang Wenjie,Han Zhenhua,Pei Jin,Pavesi Giorgio,Gong Xiaobo,Yuan Shouqi 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.1
To reduce the energy consumption of large centrifugal pumps, modified heuristic intelligent algorithms are used to directly optimize the diffuser of centrifugal pumps. Considering the hydraulic efficiency under the design condition as the optimization target, in this study, 14 geometric parameters such as the inlet diameter, outlet diameter, and leading and trailing vane angles of the diffuser are selected as design variables, and the modified particle swam optimization and gravitational search algorithm are used to directly search for optimization in the design space. The performance and loss of internal entropy production of the different models before and after optimization are compared and analyzed in detail. The results show that the global optimization ability of the modified algorithm is improved. The diffuser model changes from cylindrical to twisted, the vane wrap angle increases, and the thickness of the leading edge decreases. Under the design condition, the efficiency of modified particle swarm optimization algorithm solution is increased by 2.75% and modified gravitational search algorithm solution by 2.21%, while the power remains unchanged. Furthermore, the optimization solution has the largest lift efficiency improvement under part-load conditions. After optimization, the unstable flow in the model is improved and internal entropy production loss is reduced significantly. The interior of the diffuser is dominated by turbulent entropy production and direct entropy production under different operating conditions, and the wall entropy production accounts for the smallest proportion.