Gender-independent efficacy of mesenchymal stem cell therapy in sex hormone-deficient bone loss via immunosuppression and resident stem cell recovery

Bing-Dong Sui,Ji Chen,Xin-Yi Zhang,Tao He,Pan Zhao,Chen-Xi Zheng,Meng Li,Cheng-Hu Hu,Yan Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Osteoporosis develops with high prevalence in both postmenopausal women and hypogonadal men. Osteoporosis results in significant morbidity, but no cure has been established. Mesenchymal stem cells (MSCs) critically contribute to bone homeostasis and possess potent immunomodulatory/anti-inflammatory capability. Here, we investigated the therapeutic efficacy of using an infusion of MSCs to treat sex hormone-deficient bone loss and its underlying mechanisms. In particular, we compared the impacts of MSC cytotherapy in the two genders with the aim of examining potential gender differences. Using the gonadectomy (GNX) model, we confirmed that the osteoporotic phenotypes were substantially consistent between female and male mice. Importantly, systemic MSC transplantation (MSCT) not only rescued trabecular bone loss in GNX mice but also restored cortical bone mass and bone quality. Unexpectedly, no differences were detected between the genders. Furthermore, MSCT demonstrated an equal efficiency in rectifying the bone remodeling balance in both genders of GNX animals, as proven by the comparable recovery of bone formation and parallel normalization of bone resorption. Mechanistically, using green fluorescent protein (GFP)-based cell-tracing, we demonstrated rapid engraftment but poor inhabitation of donor MSCs in the GNX recipient bone marrow of each gender. Alternatively, MSCT uniformly reduced the CD3+T-cell population and suppressed the serum levels of inflammatory cytokines in reversing female and male GNX osteoporosis, which was attributed to the ability of the MSC to induce T-cell apoptosis. Immunosuppression in the microenvironment eventually led to functional recovery of endogenous MSCs, which resulted in restored osteogenesis and normalized behavior to modulate osteoclastogenesis. Collectively, these data revealed recipient sexually monomorphic responses to MSC therapy in gonadal steroid deficiency-induced osteoporosis via immunosuppression/anti-inflammation and resident stem cell recovery.

Microstructure and viscoelastic behavior of waterborne polyurethane/cellulose nanofiber nanocomposite

Hui Zhao,Kui-Can Li,Wei Wu,Qing Li,Yan Jiang,Bing-Xu Cheng,Chong-Xing Huang,Hua-Nan Li 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

Cellulose nanofiber (CNF) has been widely used to reinforce the mechanical properties of waterbornepolyurethane (WPU). There are, however, few works that focus on structure, rheological behavior, andcreep resistance of WPU/CNF composites. To fill this research gap, in this work, the m-CNF was obtainedby c-aminopropyltriethoxysilane modification to improve the interfacial strength of CNF and WPU, andthen it was introduced into the polyurethane matrix. Structure characterization of WPU/m-CNFnanocomposites is performed using Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction(XRD), Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The obtainedresults show that with the increase of m-CNF, the hydrogen bonding index (HBI) increased, which meanta significant improvement in the mechanical properties. The tensile strength improved by 480%. Moreover, with the increase of m-CNF content, the viscosity, storage modulus, and loss modulus of thedispersions increased and showed more obvious shear-thinning behavior. In addition, m-CNF improvedthe thermal stability and creep resistance of WPU. The creep strain of WPU decreased from 3% to 0.2%. This work offers a simply feasible way to prepare environmental friendly green nanocomposites.

Effect of TiC Nanoparticles Supported by Ti Powders on the Solidification Behavior and Microstructure of Pure Aluminum

Bingyi Zhao,Qizhou Cai,Bing Li,Jingfan Cheng 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.5

A novel grain refi ner consisting of TiC nanoparticles (NPs) supported by Ti powders (abbr. TiC/Ti refi ner) was preparedby high-energy milling. The addition of 0.5 wt% TiC/Ti refi ner converted the structure of pure Al from coarse dendrites tofi ne equiaxed grains with the average grain size of 114.7 μm, and it also increased the nucleation temperature of α(Al) from656.7 to 664.4 °C. When TiC/Ti refi ner was introduced into Al melt, the heat released from the Al–Ti reaction promoted theuniform dispersion of TiC NPs. The dissolution of the reaction product TiAl 3 released Ti atoms into the melt and thus formeda “Ti-rich transition region” around TiC NPs. The dispersive TiC NPs could act as the heterogeneous nuclei for α(Al) andthe “Ti-rich transition region” further improved the lattice orientation relationship between Al ( ̄11̄ 1 ) and TiC ( 11̄1 ) planes,which eventually resulted in the refi ning of α(Al).

In Vitro Screening for Compounds Derived from Traditional Chinese Medicines with Antiviral Activities Against Porcine Reproductive and Respiratory Syndrome Virus

( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.

Do second generation Asia corn borer (Lepidoptera: Crambidae) immigrate to corn fields from alternate habitats?

Ri-zhao Chen,Michael G. Kleinb,Qi-yun Li,Lian-Bing Li,Peng-Pei Li,Cheng-fa Sheng 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4

Many Ostrinia furnacalis (Guenée), Asian corn borer, moths inhabit non-corn fields during the second generation, and could fly into corn fields and cause damage. Therefore, during 2013–2014, O. furnacalis pheromone baited Delta traps were distributed to investigate captures in different host habitats, such as corn fields preceded by various crops, or soybean fields neighboring corn. Months captured from various host fields were evaluated in the laboratory for fecundity, female longevity and mating behavior. The sex ratio of females to males from black light baited water traps deployed in soybean and corn fields was also compared. More males were found in the soybean fields, and catches decreased in traps going fromthe field center toward a corn field. This indicates that males in soybeans were not moving into nearby corn fields. No significant differences were noted in sex ratios, life span and fecundity of females between locations. Therefore the O. furnacalis females apparently lay eggs in their initial habitat but may move to soybeans for mating.

Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates

Yi-Jing Wang,Pan Zhao,Bing-Dong Sui,Nu Liu,Cheng-Hu Hu,Ji Chen,Chen-Xi Zheng,An-Qi Liu,Kun Xuan,Ya-Ping Pan,Yan Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients’ own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention. Here, we uncovered that resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. Periodontal ligament MSCs (PDLSCs) from normal control subjects (N-PDLSCs) and periodontitis patients (P-PDLSCs) were investigated. Compared to N-PDLSCs, P-PDLSCs were less capable of forming cell aggregates, and P-PDLSC aggregates showed impaired osteogenesis and regeneration. These functional declines could be mimicked in N-PDLSCs by tumor necrosis factor alpha (TNF-α) treatment. Notably, a TNF-α-induced functional decline in N-PDLSC aggregates was rescued by RSV application. More importantly, in both N-PDLSCs and P-PDLSCs, RSV promoted cell aggregate formation and improved their osteogenic potential. Furthermore, as proven ectopically in vivo, the tissue regenerative capability of P-PDLSC aggregates was also enhanced after RSV treatment during aggregate formation in vitro. Finally, in a rat in situ regeneration model, we successfully applied both N-PDLSC aggregates and P-PDLSC aggregates to repair periodontal defects upon long-term functional improvements by RSV preconditioning. Together, our data unravel a novel methodology for using pharmacology (i.e., RSV)-based cell aggregate engineering to improve the functionality and facilitate the regeneration of MSCs from both healthy and inflammatory microenvironments, shedding light on improving the application of autologous MSC-mediated regenerative medicine.

Corrosion behavior of biodegradable magnesium alloy by MAF treatment

Xinzhe Gao,Yifan Yang,Bi Ying Shi,Chun Yu Dai,ChuanYao Zhai,Bing Cheng Zhao,Heng Bo Jiang 대한치과재료학회 2021 대한치과기재학회 학술대회 Vol.2021 No.4

Introduction. Magnesium and magnesium alloys, with mismatched degradation rates and products that are expected to support the effects being issues that need to be overcome, are hindered from clinical application. The coating preparation plays an active role in tackling this problem. The purpose of this work is to observe the corrosion control by the MAF coating in vitro and in vivo. Method. The MgF2 layer was prepared on the surface of AZ31 magnesium alloy by MAF method at 190 V DC in HF·H₂O (46%) solution. The magnesium alloy samples before and after the treatment were compared by scanning electron microscopy (SEM) observation. The coating structure and constituents were confirmed using cross-sectional SEM, energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) analysis. Potentiodynamic polarization (PDP) was utilized for electrochemical evaluation of corrosion potential and current density. 6-week-old male rats were used as experimental subjects for in vivo experiments. The volume changes and surface morphology of the corroded samples were dynamically monitored using micro-CT for 16 weeks. Results. The distribution of coating elements observed by EDS is dominated by magnesium and fluorine. The XRD analysis shows that the prepared coatings are mainly MgF2 crystals. The coating was observed by SEM to be uniformly dense and coral-like. The results of the PDP experiments showed that the coated samples had lower corrosion current density and corrosion potential. Comparison of the two groups of in vivo experiments revealed that the rate of volume change decreased in the coated samples and the density change during micro-CT observation was less than that of the uncoated group. Conclusions. Effective coatings were prepared on the surface of AZ31 magnesium alloy using the MAF method. This coating was also validated the potential to control the corrosion rate and corrosion process of magnesium alloy by both in vitro and in vivo evaluation systems.

Comparison of Autogenous Tooth Materials and Other Bone Grafts

Zhang Shuxin,Li Xuehan,Qi Yanxin,Ma Xiaoqian,Qiao Shuzhan,Cai HongXin,Zhao Bing Cheng,Jiang Heng Bo,이의석 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.3

Autogenous odontogenic materials are a new, highly biocompatible option for jaw restoration. The inorganic component of autogenous teeth acts as a scaffold to maintain the volume and enable donor cell attachment and proliferation; the organic component contains various growth factors that promote bone reconstruction and repair. The composition of dentin is similar to that of bone, which can be a rationale for promoting bone reconstruction. Recent advances have been made in the field of autogenous odontogenic materials, and studies have confirmed their safety and feasibility after successful clinical application. Autogenous odontogenic materials have unique characteristics compared with other bone-repair materials, such as the conventional autogenous, allogeneic, xenogeneic, and alloplastic bone substitutes. To encourage further research into odontogenic bone grafts, we compared the composition, osteogenesis, and development of autogenous odontogenic materials with those of other bone grafts. In conclusion, odontogenic bone grafts should be classified as a novel bone substitute.

Characteristics of Imported Malaria and Species of Plasmodium Involved in Shandong Province, China (2012-2014)

Chao Xu,Qing-Kuan Wei,Jin Li,Ting Xiao,Kun Yin,Chang-Lei Zhao,Yong-Bin Wang,Xiang-Li Kong,Gui-Hua Zhao,Hui Sun,Xin Liu,Bing-Cheng Huang 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.4

Heavy concrete shielding properties for carbon therapy

Jin-Long Wang,Jiade J Lu,Da-Jun Ding,Wen-Hua Jiang,Ya-Dong Li,Rui Qiu,Hui Zhang,Xiao-Zhong Wang,Huo-Sheng Ruan,Yan-Bing Teng,Xiao-Guang Wu,Yun Zheng,Zi-Hao Zhao,Kai-Zhong Liao,Huan-Cheng Mai,Xiao-Dong Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm³ to 4.14 g/cm³, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm³, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm³ with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.