Transcription Factor OsDOF18 Controls Ammonium Uptake by Inducing Ammonium Transporters in Rice Roots

Yunfei Wu,Wenzhu Yang,Jinhuan Wei,윤혜령,안진흥 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.3

Nitrogen is one of the most important mineral elements for plant growth. We studied the functional roles of Oryza sativa DNA BINDING WITH ONE FINGER 18 (OsDOF18) in controlling ammonium uptake. The growth of null mutants of OsDOF18 was retarded in a medium containing ammonium as the sole nitrogen source. In contrast, those mutants grew normally in a medium with nitrate as the sole nitrogen source. The gene expression was induced by ammonium but not by nitrate. Uptake of ammonium was lower in osdof18 mutants than in the wild type, while that of nitrate was not affected by the mutation. This indicated that OsDOF18 is involved in regulating ammonium transport. Among the 10 ammonium transporter genes examined here, expression of OsAMT1;1, OsAMT1;3, OsAMT2;1, and OsAMT4;1 was reduced in osdof18 mutants, demonstrating that the ammonium transporter genes function downstream of OsDOF18. Genes for nitrogen assimilation were also affected in the mutants. These results provide evidence that OsDOF18 mediates ammonium transport and nitrogen distribution, which then affects nitrogen use efficiency.

Transcription Factor OsDOF18 Controls Ammonium Uptake by Inducing Ammonium Transporters in Rice Roots

Wu, Yunfei,Yang, Wenzhu,Wei, Jinhuan,Yoon, Hyeryung,An, Gynheung Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.3

Nitrogen is one of the most important mineral elements for plant growth. We studied the functional roles of Oryza sativa DNA BINDING WITH ONE FINGER 18 (OsDOF18) in controlling ammonium uptake. The growth of null mutants of OsDOF18 was retarded in a medium containing ammonium as the sole nitrogen source. In contrast, those mutants grew normally in a medium with nitrate as the sole nitrogen source. The gene expression was induced by ammonium but not by nitrate. Uptake of ammonium was lower in osdof18 mutants than in the wild type, while that of nitrate was not affected by the mutation. This indicated that OsDOF18 is involved in regulating ammonium transport. Among the 10 ammonium transporter genes examined here, expression of OsAMT1;1, OsAMT1;3, OsAMT2;1, and OsAMT4;1 was reduced in osdof18 mutants, demonstrating that the ammonium transporter genes function downstream of OsDOF18. Genes for nitrogen assimilation were also affected in the mutants. These results provide evidence that OsDOF18 mediates ammonium transport and nitrogen distribution, which then affects nitrogen use efficiency.

The stacked over-expression of FPS, CYP71AV1 and CPR genes leads to the increase of artemisinin level in Artemisia annua L.

Yunfei Chen,Qian Shen,Yueyue Wang,Tao Wang,Shaoyan Wu,Ling Zhang,Xu Lu,Fangyuan Zhang,Weimin Jiang,Bo Qiu,Erdi Gao,Xiaofen Sun,Kexuan Tang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

Artemisinin is an endoperoxide sesquiterpenelactone isolated from the aerial parts of Artemisia annua L.,and is presently the most potent anti-malarial drug. Owingto the low yield of artemisinin from A. annua as well as thewidespread application of artemisinin-based combinationtherapy recommended by the World Health Organization,the global demand for artemisinin is substantially increasingand is therefore rendering artemisinin in short supply. An economical way to increase artemisinin production is toincrease the content of artemisinin in A. annua. In thisstudy, three key genes in the artemisinin biosynthesispathway, encoding farnesyl diphosphate synthase, amorpha-4, 11-diene C-12 oxidase and its redox partner cytochromeP450 reductase, were over-expressed in A. annuathrough Agrobacterium-mediated transformation. Thetransgenic lines were confirmed by Southern blotting andthe over-expressions of the genes were demonstrated byreal-time PCR assays. The HPLC analysis showed that theartemisinin contents in transgenic lines were increasedsignificantly, with the highest one found to be 3.6-foldhigher (2.9 mg/g FW) than that of the control. Theseresults demonstrate that multigene engineering is aneffective way to enhance artemisinin content in A. annua.

Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.

Uncertainty analysis of containment dose rate for core damage assessment in nuclear power plants

Guohua Wu,Jiejuan Tong,Yan Gao,Liguo Zhang,Yunfei Zhao 한국원자력학회 2018 Nuclear Engineering and Technology Vol.50 No.5

One of the most widely used methods to estimate core damage during a nuclear power plant accident iscontainment radiation measurement. The evolution of severe accidents is extremely complex, leading touncertainty in the containment dose rate (CDR). Therefore, it is difficult to accurately determine coredamage. This study proposes to conduct uncertainty analysis of CDR for core damage assessment. First,based on source term estimation, the Monte Carlo (MC) and point-kernel integration methods were usedto estimate the probability density function of the CDR under different extents of core damage in accidentscenarios with late containment failure. Second, the results were verified by comparing the resultsof both methods. The point-kernel integration method results were more dispersed than the MC results,and the MC method was used for both quantitative and qualitative analyses. Quantitative analysisindicated a linear relationship, rather than the expected proportional relationship, between the CDR andcore damage fraction. The CDR distribution obeyed a logarithmic normal distribution in accidents with asmall break in containment, but not in accidents with a large break in containment. A possible applicationof our analysis is a real-time core damage estimation program based on the CDR

Back-Calculation Method of Rock Mass Pressure in a Shallow-Buried Super Large-Span Tunnel Using Upper-Bench CD Method

Yanbin Luo,Yunfei Wu,Jianxun Chen,Fangfang Dong,Weiwei Liu,Lijun Chen,Yao Li,Zhou Shi 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.1

Rock mass pressure has always been a research hotspot in the field of tunnel engineering, especially in the super large-span tunnel, which is characterized by flat section, large excavation span, and complex stress field. Based on the Letuan Tunnel (a bi-directional tunnel with eight traffic lanes) of Binlai expressway expansion project in Shandong Province, China, this paper focused on the calculation method of rock mass pressure and the evolution law of load release in the construction process of the super large-span tunnel excavated by upper-bench central diaphragm (CD) method. Based on field measured data of Letuan Tunnel, the deformation behavior of primary lining and the distribution state of rock mass pressure during the tunnel construction were analyzed. According to the bearing mode of supporting structure, the mechanical models of different construction stages were established. Then, the rock mass pressures in different construction stages were back-calculated using mechanical models and compared with the measured values, and the evolution law of load release during the tunnel construction was discussed. The study results show that the tunnel deformation and rock mass pressure were significantly affected by the construction process and support form, and the excavation span was the key factor affecting the stability of rock mass. For the shallow-buried super large-span tunnel constructed by upper-bench CD method, the primary support of upper bench was under eccentric pressure. The comparison between the back-calculated value and the field measured value indicated that they were similar, and the average relative error was 17.23%. According to the concept of load release coefficient proposed in this paper, the load release coefficient after the pilot tunnel ahead (Part I) excavation reached 63%, and the load release coefficient after the pilot tunnel behind excavation (Part II) was 37%, which means that the rock mass pressure of Part I is increased about 59% due to the excavation of Part II.

Identification of root-preferential transcription factors in rice by analyzing GUS expression patterns of T-DNA tagging lines

Jinhuan Wei,Yunfei Wu,Lae-Hyeon Cho,Jinmi Yoon,Heebak Choi,Hyeryung Yoon,Ping Jin,Jakyung Yi,Yang-Seok Lee,Hee Joong Jeong,Jungil Yang,Gynheung An 한국식물학회 2017 Journal of Plant Biology Vol.60 No.3

T-DNA tagging lines are useful for analyzing the functions of genes and regulatory elements. We have previously generated approximately 100,000 insertional mutants in japonica rice (Oryza sativa), using T-DNA vectors carrying the promoter-less GUS reporter gene. In this study, we conducted GUS assays of seedlings from 430 lines in which TDNA was inserted into transcription factor genes. Among the 75 lines that showed GUS signals, nine displayed an endospermpreferential expression pattern; two lines demonstrated GUS signals in both endosperm and roots; 21 lines had GUS expression mainly in leaves; 19 lines showed GUS signal in both leaves and roots; and 24 lines expressed GUS predominantly in the roots. Co-segregation analyses of 49 homozygous lines indicated that the GUS expression patterns observed from 38 lines were due to the T-DNA insertion. We also identified fusion transcripts between tagged genes and the GUS reporter in six lines. Quantitative RT-PCR confirmed that the GUS expression patterns of those tagged lines indeed represent organ- and tissue-preferential expression of the tagged genes. The GUS-tagged transcription factor lines identified here will be useful for functional analysis of these candidates.

Real-time detection of the nanoparticle induced phytotoxicity in rice root tip through the visible red emissions of Eu³⁺ ions

Du, Peng,Wu, Yunfei,Yu, Jae Su The Royal Society of Chemistry 2018 PHOTOCHEMICAL AND PHOTOBIOLOGICAL SCIENCES Vol.17 No.4

<P>Phytotoxicity is one of the most important factors involved in the reduction of crop production. With the introduction of NaBiF4 nanoparticles, the effect of the particle size (>50 nm) on rice development was systematically studied. Through the exogenous treatment of multiple concentrations of nanoparticles, the primary root length, lateral root number, and lateral root length were significantly inhibited under higher content of nanoparticles, but more crown root formation was induced, which might be due to phytotoxicity. With the help of the red emission of the Eu<SUP>3+</SUP>-activated NaBiF4 nanoparticles, we could infer that the nanoparticles were accumulated in the root tip cells in the division and elongation zone but not in the mature region. Additionally, the investigation on the influence of the studied nanoparticles on the gene level and the expression of phytotoxicity related genes was performed to further identify the effect of the nanoparticles on the rice root development. These results potentially explain the effect of larger nanoparticles on phytotoxicity in the plant roots.</P>

MICROWAVE-ASSISTED HYDROTHERMAL SYNTHESIS AND LUMINESCENCE PROPERTIES OF Eu3+-DOPED CdTe QUANTUM DOTS

HU CHEN,YUNFEI LIU,YINONG LU,HONGMING WU,HAO QIAN,BAOSHUAI HUANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.4

The Eu3+-doped CdTe quantum dots (QDs) have been successfully synthesized in aqueous phase through microwave-assisted hydrothermal method. The as-prepared products were characterized by X-ray diffraction (XRD) technique, high-resolution transmission electron microscope (HRTEM), energy dispersive spectrum (EDS), X-ray photoclectron spectrum (XPS), UV-visble (UV-Vis) absorption spectrum and photoluminescence (PL). As the results show, the average sizes of the cubic phase CdTe and CdTe:1%Eu3+ are an average size of 3.7 nm and 3.5 nm, respectively. The average lattice parameter of CdTe:Eu3+ decreases with increasing Eu3+ ions concentration. Furthermore, compared to green emission of the host CdTe, the CdTe:1%Eu3+ presents additional orange fluorescence emission of Eu3+ ions. Recombination luminescence color is almost located in the yellow region of the CIE 1931, and the CIE 1931 coordinate is (0.448, 0.513). The effect of different Eu3+ doping concentration on the luminescence of CdTe:Eu3+ QDs was discussed in detail. It suggests that CdTe:Eu3+ QDs are potential phosphors for white light-emitting materials.

The Method of Aiming towards the Normal Direction for Robotic Drilling

Yuhao Gao,Dan Wu,Yunfei Dong,Xinguo Ma,Ken Chen 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.6

During the robotic drilling of an aircraft assembly, the normal directions of the workpieces are to be measured to guide the robot by aiming the spindle at the real normal directions of the curved surfaces. This paper describes the development of a method of using four laser displacement sensors to measure the normal direction and the implementation of the proposed method in a robotic drilling system to meet industrial application requirements. First, a novel mechanical structure is introduced to achieve a higher installation accuracy for the laser displacement sensors with a micro adjusting method. Next, the hardware structures and a convenient method of calibrating the sensors are introduced. To accommodate the complexity of the industrial circumstance, a procedure of measuring and adjusting is developed with consideration of both an angular deviation threshold and a limited adjusting iteration. Finally, the experiments of measuring and adjusting iteratively show that two iterations can achieve a satisfactory angular deviation of 0.1° for planar workpieces and three iterations are required for curved workpieces, with the perpendicularity errors of the drilled holes being less than 0.3°. The results can meet the required perpendicularity accuracy of 0.5°.