Why does SLIPS inhibit P.aeruginosa initial adhesion in static condition?

Yuanyuan Shen,Yihan Sun,Peng Wang,Dun Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.124 No.-

Slippery liquid-infused porous surfaces (SLIPSs) have distinguished themselves in inhibiting bacteriaattachment and biofilm development in static conditions. However, underlying antifouling mechanisms,especially from gene level in bioinformatics, is still lacking. In this work, we investigated the initialattachment difference of Pseudomonas aeruginosa PAO1 on polydimethylsiloxane (PDMS) surface andthe infused silicone slippery surface (i-PDMS). RNA sequencing (RNA-seq) was used to investigate the differencesin the expression of PAO1 gene on elastomer surface during initial adhesion before and after oilinjection. Compared with PDMS, bacterial attachment on i-PDMS was remarkably decreased 98.0 ± 0.7 %within 10 mins. And the antifouling ability of i-PDMS significantly outperformed PDMS throughout theentire culture period of PAO1 (14 days) in static conditions. RNA-seq reveals that the down-regulatedPA1382 of PAO1 in bulk near the i-PDMS surfaces may inhibit bacterial initial adhesion. PA1382 geneencodes type II secretion outer membranes (OM) secretin, also known as type II secretion system(T2SS) protein GspD, which is involved in regulating the opening or closing of exoprotein channels, influencingbacterial adhesion and biofilm formation by controlling the secretion of toxins or effectors. Ourfindings provide a deeper understanding of the mechanism by which SLIPS inhibits initial bacterialadhesion.

Corticosterone Regulates the Expression of Neuropeptide Y and Reelin in MLO-Y4 Cells

Yuanyuan Ma,Hang Wang,Xiangnan Wu,Jing Fu,Jiefei Shen,Xiaoyu Li 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.6

Osteocytes that have a dendritic appearance are widely believed to form a complex cellular network system and play crucial roles in mechanotransduction as a principal bone mechanosensor, which is the basis of their neuronal-like biology, as previously reported. Neuropeptide Y (NPY) and reelin mRNA, which are brain-specific neurogenic markers, have been identified in osteocytes. However, changes in the production of NPY and reelin in response to specific biochemical stimulation are unknown. In this study, we investigated the in vitro effect of corticosterone, one of the endogenous glucocorticoids, on the expression of NPY and reelin in the MLO-Y4 osteocyte cell line. Cells were treated with corticosterone at different concentrations (10-9 M-10-5 M) for 1, 3, 6, 12 and 24 h. As revealed, corticosterone reduced the MLO-Y4 cell viability and pro-liferation in a dose- and time-dependent manner based on an MTT assay and a Vi-CELL analyzer. The cells were then incubated with corticosterone (10-6 M), and the NPY and reelin expression levels were detected at 1, 3, 6, 12 and 24 h using real-time PCR and Western blot analysis. These results demonstrated that at the gene and the protein levels, corticosterone significantly upregulated the NPY and reelin expression in a time-dependent manner. The application of a glucocorticoid receptor antagonist, RU486, reversed the reduced cell viability and the increased expression of NPY and reelin that were caused by corticosterone. To the best of our knowledge, this is the first report to verify that corticosterone regulates the NPY and reelin expression in osteocytes.

Study on the Influence of Variation of Contact Arc Zone on the Single-Pass Sawing of Sapphire Wafer

Jianyun Shen,Lang Lu,Yuanyuan Gong,Xipeng Xu 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.9

Single-pass sawing (SPS), a significant cutting process widely used in the manufacturing of optical components in optoelectronic fields, which can greatly improve the quality of products by reducing the proportion of edge chipping. In this paper, the mechanism of SPS was introduced by a novel approach of deep analysis of the variation of the contact arc zone. Setting sapphire wafers as the workpiece, this study performed the comparative experiments in three specific arc zones, which would further make a thorough inquiry on the related machining mechanism. The difficulty degree of machining and the topography of surface quality during SPS process were also analyzed. The difficulty degree of machining was reflected by the sawing force ratio, and the machining surfaces of sapphire wafers were compared by SEM and Digital Microscope. The results indicate that the lower the arc is, the easier it can be machined during SPS. And the best surface quality appears in Arc 2 (arc angle = 20°), which can be set as an excellent reference for the machining of other materials during SPS. The single-pass sawing mechanism is also derived on the basis of the traditional cutting mechanism model, which will further serve as useful references to both the industrial machining and the academia.

Amelioration of Bleomycin-induced Pulmonary Fibrosis of Rats by an Aldose Reductase Inhibitor, Epalrestat

Xianwei Li,Yuanyuan Shen,Yining Lu,Jieren Yang 대한생리학회-대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.5

Aldose reductase (AR) is known to play a crucial role in the mediation of diabetic and cardiovascular complications. Recently, several studies have demonstrated that allergen-induced airway remodeling and ovalbumin-induced asthma is mediated by AR. Epalrestat is an aldose reductase inhibitor that is currently available for the treatment of diabetic neuropathy. Whether AR is involved in pathogenesis of pulmonary fibrosis and whether epalrestat attenuates pulmonary fibrosis remains unknown. Pulmonary fibrosis was induced by intratracheal instillation of bleomycin (5 mg/kg) in rats. Primary pulmonary fibroblasts were cultured to investigate the proliferation by BrdU incorporation method and flow cytometry. The expression of AR, TGF-β₁, α-SMA and collagen I was analyzed by immunohis-tochemisty, real-time PCR or western blot. In vivo, epalrestat treatment significantly ameliorated the bleomycin-mediated histological fibrosis alterations and blocked collagen deposition concomitantly with reversing bleomycin-induced expression up-regulation of TGF-β₁, AR, α-SMA and collagen I (both mRNA and protein). In vitro, epalrestat remarkably attenuated proliferation of pulmonary fibroblasts and expression of α-SMA and collagen I induced by TGF-β₁, and this inhibitory effect of epalrestat was accompanied by inhibiting AR expression. Knockdown of AR gene expression reversed TGF-β₁-induced proliferation of fibroblasts, up-regulation of α-SMA and collagen I expression. These findings suggest that AR plays an important role in bleomycin-induced pulmonary fibrosis, and epalrestat inhibited the progression of bleomycin-induced pulmonary fibrosis is mediated via inhibiting of AR expression.

Amelioration of Bleomycin-induced Pulmonary Fibrosis of Rats by an Aldose Reductase Inhibitor, Epalrestat

Li, Xianwei,Shen, Yuanyuan,Lu, Yining,Yang, Jieren The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.5

Aldose reductase (AR) is known to play a crucial role in the mediation of diabetic and cardiovascular complications. Recently, several studies have demonstrated that allergen-induced airway remodeling and ovalbumin-induced asthma is mediated by AR. Epalrestat is an aldose reductase inhibitor that is currently available for the treatment of diabetic neuropathy. Whether AR is involved in pathogenesis of pulmonary fibrosis and whether epalrestat attenuates pulmonary fibrosis remains unknown. Pulmonary fibrosis was induced by intratracheal instillation of bleomycin (5 mg/kg) in rats. Primary pulmonary fibroblasts were cultured to investigate the proliferation by BrdU incorporation method and flow cytometry. The expression of AR, TGF-${\beta}_1$, ${\alpha}$-SMA and collagen I was analyzed by immunohistochemisty, real-time PCR or western blot. In vivo, epalrestat treatment significantly ameliorated the bleomycin-mediated histological fibrosis alterations and blocked collagen deposition concomitantly with reversing bleomycin-induced expression up-regulation of TGF-${\beta}_1$, AR, ${\alpha}$-SMA and collagen I (both mRNA and protein). In vitro, epalrestat remarkably attenuated proliferation of pulmonary fibroblasts and expression of ${\alpha}$-SMA and collagen I induced by TGF-${\beta}_1$, and this inhibitory effect of epalrestat was accompanied by inhibiting AR expression. Knockdown of AR gene expression reversed TGF-${\beta}_1$-induced proliferation of fibroblasts, up-regulation of ${\alpha}$-SMA and collagen I expression. These findings suggest that AR plays an important role in bleomycin-induced pulmonary fibrosis, and epalrestat inhibited the progression of bleomycin-induced pulmonary fibrosis is mediated via inhibiting of AR expression.

An Efficient and Secure Authentication Scheme with Session Key Negotiation for Timely Application of WSNs

Jiping Li,Yuanyuan Zhang,Lixiang Shen,Jing Cao,Wenwu Xie,Yizheng,Shouyin Liu 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.3

For Internet of Things, it is more preferred to have immediate access to environment information from sensor nodes (SNs) rather than from gateway nodes (GWNs). To fulfill the goal, mutual authentication scheme between user and SNs with session key (SK) negotiation is more suitable. However, this is a challenging task due to the constrained power, computation, communication and storage resources of SNs. Though lots of authentication schemes with SK negotiation have been designed to deal with it, they are still insufficiently secure and/or efficient, and some even have serious vulnerabilities. Therefore, we design an efficient secure authentication scheme with session key negotiation (eSAS2KN) for wireless sensor networks (WSNs) utilizing fuzzy extractor technique, hash function and bitwise exclusive-or lightweight operations. In the eSAS2KN, user and SNs are mutually authenticated with anonymity, and an SK is negotiated for their direct and instant communications subsequently. To prove the security of eSAS2KN, we give detailed informal security analysis, carry out logical verification by applying BAN logic, present formal security proof by employing Real-Or-Random (ROR) model, and implement formal security verification by using AVISPA tool. Finally, computation and communication costs comparison show the eSAS2kN is more efficient and secure for practical application.

Properties of positive solutions for the fractional Laplacian systems with positive-negative mixed powers

Zhongxue Lu,Mengjia Niu,Yuanyuan Shen,Anjie Yuan 대한수학회 2024 대한수학회지 Vol.61 No.3

In this paper, by establishing the direct method of moving planes for the fractional Laplacian system with positive-negative mixed powers, we obtain the radial symmetry and monotonicity of the positive solutions for the fractional Laplacian systems with positive-negative mixed powers in the whole space. We also give two special cases.

An Improved Anonymous Remote user Authentication Scheme with Key Agreement based on Dynamic Identity

Yajuan Shi,Han Shen,Yuanyuan Zhang,Jianhua Chen 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.5

To keep the pace with the development of internet technology, remote user authentication techniques become more and more important to protect user’s privacy. Recently, Kumari, et al., presented an improved remote user authentication scheme with key agreement based on dynamic-identity using smart card. This scheme allows legal users to change the password at his will without the need to connect the server. They claimed that their scheme could resist smart card stolen or loss attack, user impersonation and server masquerading attack, and provide user anonymity and untraceability and so on. However, our research indicates that their scheme is completely unsafe. Furthermore, the scheme can’t provide the proper mutual authentication. In this manuscript, we will propose a new scheme, which can withstand those attacks mentioned above and provide the perfect user anonymity and forward secrecy. Security analysis makes it clear that the improved scheme apparently is more secure and practical.

Ginsenoside Rb1 attenuates methamphetamine (METH)-induced neurotoxicity through the NR2B/ERK/CREB/BDNF signalings in vitro and in vivo models

Genmeng Yang,Juan Li,Yanxia Peng,Baoyu Shen,Yuanyuan Li,Liu Liu,Chan Wang,Yue Xu,Shucheng Lin,Shuwei Zhang,Yi Tan,Huijie Zhang,Xiaofeng Zeng,Qi Li,Gang Lu 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3

This study investigates the effects of ginsenoside Rb1 (GsRb1) on methamphetamine (METH)-induced toxicity in SH-SY5Y neuroblastoma cells and METH-induced conditioned place preference (CPP)in adult Sprague-Dawley rats. It also examines whether GsRb1 can regulate these effects through theNR2B/ERK/CREB/BDNF signaling pathways. Methods: SH-SY5Y cells were pretreated with GsRb1 (20 mM and 40 mM) for 1 h, followed by METHtreatment (2 mM) for 24 h. Rats were treated with METH (2 mg/kg) or saline on alternating days for 10days to allow CPP to be examined. GsRb1 (5, 10, and 20 mg/kg) was injected intraperitoneally 1 h beforeMETH or saline. Western blot was used to examine the protein expression of NR2B, ERK, P-ERK, CREB, PCREB, and BDNF in the SH-SY5Y cells and the rats' hippocampus, nucleus accumbens (NAc), and prefrontal cortex (PFC). Results: METH dose-dependently reduced the viability of SH-SY5Y cells. Pretreatment of cells with 40mM of GsRb1 increased cell viability and reduced the expression of METH-induced NR2B, p-ERK, p-CREBand BDNF. GsRb1 also attenuated the expression of METH CPP in a dose-dependent manner in rats. Further, GsRb1 dose-dependently reduced the expression of METH-induced NR2B, p-ERK, p-CREB, andBDNF in the PFC, hippocampus, and NAc of rats. Conclusion: GsRb1 regulated METH-induced neurotoxicity in vitro and METH-induced CPP through theNR2B/ERK/CREB/BDNF regulatory pathway. GsRb1 could be a therapeutic target for treating METHinduced neurotoxicity or METH addiction.

Corrigendum to "Ginsenoside Rb1 attenuates methamphetamine (METH)-induced neurotoxicity through the NR2B/ERK/CREB/BDNF signalings in vitro and in vivo models"

Genmeng, Yang,Juan, Li,Yanxia, Peng,Baoyu, Shen,Yuanyuan, Li,Liu, Liu,Chan, Wang,Yue, Xu,Shucheng, Lin,Shuwei, Zhang,Yi, Tan,Huijie, Zhang,Xiaofeng, Zeng,Qi, Li,Gang, Lu The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2