Deregulation of miR-519a, 153, and 485-5p and its clinicopathological relevance in ovarian epithelial tumours

Kim, Tae Heon,Kim, Yu Kyung,Kwon, Youngdo,Heo, Jin Hyung,Kang, Haeyoun,Kim, Gwangil,An, Hee Jung Blackwell Publishing Ltd 2010 Histopathology Vol.57 No.5

<P>Kim T H, Kim Y K, Kwon Y, Heo J H, Kang H, Kim G & An H J(2010) <I>Histopathology</I><B>57</B>, 734–743 <B>Deregulation of miR-519a, 153, and 485-5p and its clinicopathological relevance in ovarian epithelial tumours</B></P><P>Aims: </P><P>The pathological and clinical significance of aberrant miRNA expression in ovarian tumours has yet to be adequately documented. The aim of this study was to assess the differences in miRNA expression of human ovarian tumours according to histological subtype, and to determine whether miRNAs are potential diagnostic and prognostic markers in ovarian cancers.</P><P>Method and results: </P><P>The miRNA expression profiles of 103 human ovarian tumours were evaluated. Via a bead-based miRNA microarray, five aberrant miRNAs were selected which were expressed differentially in malignant serous tumours from borderline and benign ovarian tumours, including miRNA (miR)-519a, miR-18b (up-regulation) and miR-153, miR-511 and miR-485-5p (down-regulation). We conducted quantitative real-time reverse transcription–polymerase chain reaction (qRT–PCR) in order to confirm that these miRNAs are differentially expressed in different histological subtypes of ovarian tumours, and compared the expression profiles of these miRNAs between different clinical subsets. The expression of these miRNAs was correlated with clinicopathological parameters. miR-519a, miR-153 and miR-485-5p were differentially expressed in four major histotypes of ovarian cancers (<I>P </I>< 0.05), which suggests that they might be of potential importance as diagnostic biomarkers. Down-regulation of miR-153 and miR-485-5p was correlated significantly with FIGO grade 3 (<I>P </I>< 0.05). Down-regulation of miR-153 and up-regulation of miR-519a were correlated significantly with advanced clinical stage (<I>P </I>< 0.05). The results of Kaplan–Meier survival analysis indicated that the higher expression of miR-519a in late stage serous carcinoma was associated significantly with poor progression-free survival (<I>P </I>= 0.0058).</P><P>Conclusions: </P><P>A significant correlation was detected between the deregulation of specific types of miRNAs, such as miR-519a, miR-153 and miR-485-5p, and clinical variables as well as histological subtypes in ovarian cancers. Hence, these miRNAs may perform functions as diagnostic or prognostic biomarkers.</P>

Enhanced Superconductivity of Sr₂RuO₄ and La<SUB>2-x</SUB>Sr<SUB>x</SUB>CuO₄ Thin Films by Atomic-Scale Interface Engineering

Jinkwon Kim,Junsik Mun,Youngdo Kim,Bongju Kim,Jeong Rae Kim,Lingfei Wang,Miyoung Kim,Changyoung Kim,Jason W. A. Robinson,Yoshiteru Maeno,Tae Won Noh 한국자기학회 2021 한국자기학회 학술연구발표회 논문개요집 Vol.31 No.2

Ruddlesden-Popper (RP) phase oxides (An+1BnO3n+1, n = 1, 2, ...) have been spotlighted with versatile physical properties such as high-temperature superconductivity, colossal magnetoresistance. These emergent phenomena provide a platform for novel oxide-based electronic devices including spintronics application. However, high-quality RP-phase thin film growth has been disturbed by extended structural defects, such as out-of-phase boundaries (OPBs). OPB is a translational boundary between neighboring unit cells, shifted in a specific crystallographic direction. For instance, if RP-phase thin films grown on ABO₃ perovskite substrates, the structural mismatch between film and substrates induces a crystallographic shift in the c-axis direction, thus OPBs form at the film-substrate interface. Since OPB formation hampers the physical properties of RP-phase thin films, the suppression of the structural defects is highly required to carry out the high-performance RP-phase based functional devices. In this study, we suppressed OPB suppression in RP-phase oxide thin films by atomic-scale interface engineering. As model systems, the unconventional superconductor Sr₂RuO₄ (bulk Tc ~ 1.5 K) and La2-xSrxCuO₄ (bulk Tc ~ 39 K) thin films were employed. Despite the structural similarities between films and substrates, Sr2RuO4 and La2-xSrxCuO₄ films exhibited huge OPB formations. By controlling the atomic-scale interface engineering, the OPBs were significantly suppressed in the film structure. Notably, these OPB-free Sr₂RuO₄ and La2-xSrxCuO₄ thin films exhibited highly enhanced superconductivity than the film with huge OPB formation. Our study suggests a comprehensive method to suppress OPB formation in RP thin films, enabling superconducting spintronics devices based on the unconventional superconductivity.

On the Estimation of Heritability with Family-Based and Population-Based Samples

Kim, Youngdoe,Lee, Young,Lee, Sungyoung,Kim, Nam Hee,Lim, Jeongmin,Kim, Young Jin,Oh, Ji Hee,Min, Haesook,Lee, Meehee,Seo, Hyeon-Jeong,Lee, So-Hyun,Sung, Joohon,Cho, Nam H.,Kim, Bong-Jo,Han, Bok-Ghee Hindawi Publishing Corporation 2015 BioMed research international Vol.2015 No.-

<P>For a family-based sample, the phenotypic variance-covariance matrix can be parameterized to include the variance of a polygenic effect that has then been estimated using a variance component analysis. However, with the advent of large-scale genomic data, the genetic relationship matrix (GRM) can be estimated and can be utilized to parameterize the variance of a polygenic effect for population-based samples. Therefore narrow sense heritability, which is both population and trait specific, can be estimated with both population- and family-based samples. In this study we estimate heritability from both family-based and population-based samples, collected in Korea, and the heritability estimates from the pooled samples were, for height, 0.60; body mass index (BMI), 0.32; log-transformed triglycerides (log TG), 0.24; total cholesterol (TCHL), 0.30; high-density lipoprotein (HDL), 0.38; low-density lipoprotein (LDL), 0.29; systolic blood pressure (SBP), 0.23; and diastolic blood pressure (DBP), 0.24. Furthermore, we found differences in how heritability is estimated—in particular the amount of variance attributable to common environment in twins can be substantial—which indicates heritability estimates should be interpreted with caution.</P>

Enhanced functionalities in Ruddlesden-Popper oxide thin films employing single-terminated substrate surfaces

Jinkwon Kim,Youngdo Kim,Junsik Mun,Jeong Rae Kim,Miyoung Kim,Changyoung Kim,Tae Won Noh 한국자기학회 2021 한국자기학회 학술연구발표회 논문개요집 Vol.31 No.1

Over the past decades, Ruddlesden-Popper (RP) phase oxides (A_n+1B_nO_3n+1, n = 1, 2, ...) have been spotlighted with advantageous physical properties such as high-temperature superconductivity, giant magnetoresistance. ^[1-2] These emergent phenomena provide a platform for novel oxide-based electronic devices including spintronics application. However, high-quality RP-phase thin film growth has been disturbed by extended structural defects, so-called out-of-phase boundaries (OPBs).^[3] OPB is a translational boundary between neighboring unit cells, shifted in a specific crystallographic direction. For instance, if RP-phase thin films grown on ABO₃ perovskite substrates, the structural mismatch between film and substrates induces a crystallographic shift in the c-axis direction, thus OPBs form at the film-substrate interface. Since OPB formation hampers the physical properties of RP-phase thin films, the suppression of the structural defects is highly required to carry out the high-performance RP-phase based functional devices.^[4-5] In this study, we suppressed OPB suppression in RP-phase oxide thin films by using single-terminated LaSrAlO4 substrate (n = 1 RP phase, a = b= 3.756 Å and c =12.636 Å). As a model system, the high-T_c cup rates superconductor La_1.85Sr_0.15CuO4 thin film (n = 1 RP phase, a = b= 3.777 Å and c =13.226 Å) was employed. Despite the structural similarities between films and substrates, the La_2-xSr_xCuO₄ films exhibited huge OPB formations when deposited on mixed-terminated LaSrAlO4 substrate. In contrast, when the La_2-xSr_xCuO₄ films were deposited on single-terminated LaSrAlO4 substrates, the OPBs were significantly suppressed in the film structure. Notably, these OPB-free La_2-xSr_xCuO₄ films exhibited highly enhanced superconductivity (T_c^zero ~ 30 K) than the film with huge OPB formation (T_c^zero ~ 5 K) under the same thickness (~ 6.5 nm). Our study suggests a comprehensive method to suppress OPB formation in RP thin films, enabling superconducting spintronics devices based on RP-phase high-T_c superconductors.[5-6]

Efficacy and Safety of Infliximab in Intestinal Behçet’s Disease: A Multicenter, Phase 3 Study (BEGIN)

Cheon Jae Hee,Kim Hyun-Soo,Han Dong Soo,Kim Sung Kook,Shin Sung Jae,Kim Joo Sung,Ye Byong Duk,Song Geun Am,Lee YoungJa,Kim Youngdoe,Lee Yoosun,Kim Won Ho 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.5

Background/Aims: To date, there is no prospective study that specifically investigated the efficacy of infliximab in intestinal Behçet’s disease (BD). This study evaluated the efficacy of infliximab in patients with moderate-to-severe active intestinal BD that are refractory to conventional therapies. Methods: This phase 3, interventional, open-label, single-arm study evaluated clinical outcomes of infliximab treatment in patients with moderate-to-severe intestinal BD. The coprimary endpoints were clinical response, decrease in disease activity index for intestinal BD (DAIBD) score ≥20 from weeks 0 to 8 for the induction therapy and week 32 for the maintenance therapy. Results: A total of 33 patients entered the induction therapy and were treated with infliximab 5 mg/kg intravenously at weeks 0, 2, and 6. The mean DAIBD score changed from 90.8±40.1 at week 0 to 40.3±36.4 at week 8, with a significant mean change of 50.5±36.4 (95% confidence interval, 37.5 to 63.4; p<0.001). Thirty-one (93.9%) continued to receive 5 mg/kg infliximab every 8 weeks during the maintenance therapy. The mean change in the DAIBD score after the maintenance therapy was statistically significant (61.5±38.5; 95% confidence interval, 46.0 to 77.1; p<0.001, from weeks 0 to 32). The proportion of patients who maintained a clinical response was 92.3% at week 32. No severe adverse reactions occurred during the induction and maintenance therapies. Conclusions: This study provided evidence that infliximab 5 mg/kg induction and maintenance therapies are efficacious and well-tolerated in patients with moderate-to-severe active intestinal BD. (ClinicalTrials.gov identifier: NCT02505568)

Quality of Life in Newly Diagnosed Moderate-to-Severe Ulcerative Colitis: Changes in the MOSAIK Cohort Over 1 Year

Jo Ik Hyun,Lee Kang-Moon,Kim Dae Bum,Kim Ji Won,Lee Jun,Jeen Yoon Tae,Kim Tae-Oh,Kim Joo Sung,Park Jae Jun,Hong Sung Noh,Park Dong Il,김현수,Lee Yoo Jin,Kim Youngdoe 거트앤리버 소화기연관학회협의회 2022 Gut and Liver Vol.16 No.3

Background/Aims: Improving quality of life has been gaining importance in ulcerative colitis (UC) management. The aim of this study was to investigate changes in health-related quality of life (HRQL) and related factors in patients with moderate-to-severe UC. Methods: A multicenter, hospital-based, prospective study was performed using a Moderateto- Severe Ulcerative Colitis Cohort in Korea (the MOSAIK). Changes in HRQL, evaluated using the 12-Item Short Form Health Survey (SF-12) and Inflammatory Bowel Disease Questionnaire (IBDQ), were analyzed at the time of diagnosis and 1 year later. Results: In a sample of 276 patients, the mean age was 38.4 years, and the majority of patients were male (59.8%). HRQL tended to increase in both the IBDQ and SF-12 1 year after diagnosis. A higher partial Mayo score was significantly related to poorer HRQL on the IBDQ and SF-12 in a linear mixed model (p<0.01). Inflammatory markers such as C-reactive protein (CRP) or erythrocyte sedimentation rate also showed a negative correlation on HRQL (p<0.05). Patients whose IBDQ score improved by 16 or more (71.2%) in 1 year were younger, tended to be nonsmokers, and had a lower partial Mayo score and CRP than those whose IBDQ score did not. There was no significant association between HRQL and disease extent, treatments at diagnosis, or the highest treatment step during the 1-year period. Conclusions: Optimally controlled disease status improves HRQL in patients with moderate-tosevere UC. The partial Mayo score and inflammatory markers may be potential indicators reflecting the influence of UC on patient`s daily lives.

Symmetry-preserving strain engineering of Hundness and Mottness in a two-dimensional correlated system

Eun Kyo Ko,Sungsoo Hahn,Changhee Sohn,Sangmin Lee,Byungmin Sohn,Jeong Rae Kim,Jaeseok Son,Youngdo Kim,Donghan Kim,Miyoung Kim,Choong H. Kim,Changyoung Kim,Tae Won Noh 한국자기학회 2022 한국자기학회 학술연구발표회 논문개요집 Vol.31 No.2

Molecularly imprinted fullerene-silica nanocomposite particles for sensitive and selective recognition of diethylstilbestrol

Kim, Youngdo,Jeon, Jae Bum,Chang, Ji Young Elsevier 2016 Materials letters Vol.182 No.-

<P><B>Abstract</B></P> <P>A highly sensitive, molecularly imprinted fluorescent sensor was fabricated using a C<SUB>60</SUB> fullerene as the signal transducer and silica as the imprinting matrix. The incorporation of C<SUB>60</SUB> and the formation of diethylstilbestrol (DES) imprinted sites in the silica network were achieved by the sol–gel method. C<SUB>60</SUB> has a narrow band gap between the ground and excited states, resulting in a weak photoluminescence. However, C<SUB>60</SUB> showed a strong emission when it was incorporated into a molecularly imprinted silica matrix. DES imprinted fullerene-silica nanocomposite particles (MIFSNCs) showed an intense fluorescence emission with the peak maximum at ∼600nm. The fluorescence intensity of MIFSNCs significantly decreased with increasing DES concentration. MIFSNCs exhibited a linear Stern–Volmer relationship for DES and its structural analogs. The quenching constant of MIFSNCs for DES was about five times higher than those for DES analogs, indicating the highly selective recognition property.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A molecularly imprinted sensor is fabricated using C<SUB>60</SUB> as a signal transducer. </LI> <LI> Delicate binding sites are formed in the silica network by the sol–gel chemistry. </LI> <LI> C<SUB>60</SUB> fullerenes form a nanoparticulate cluster that shows quantum confinement effects. </LI> <LI> The sensor shows concentration dependent fluorescence quenching for the analyte. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A highly sensitive, molecularly imprinted fluorescent sensor is fabricated using a C<SUB>60</SUB> fullerene as a signal transducer and silica as an imprinting matrix.</P> <P>[DISPLAY OMISSION]</P>

Scalable synthesis of the C14–C23 fragment of Eribulin and Halichondrin B

Kim U Bin,Samala Srinivas,Kim Namhyeon,Bogonda Ganganna,Lago‐Santomé Hugo,Jeong Youngdo,Kim Jin,Jung Jaehun,Jeon Sung‐Hyun,Lee Seung Jong,신현석 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.12

A novel, scalable approach to the C14–C23 fragment of eribulin mesylate is disclosed. Key 2,3-Wittig rearrangement is strategically effected via [Rh] mediated decomposition of 1,2,3-triazole intermediate to derive the 2,5-trans-tetrahydrofuran motif, enabling multi-kilogram access to the desired C14–C23 fragment.

High Ion Conducting Nanohybrid Solid Polymer Electrolytes <i>via</i> Single-Ion Conducting Mesoporous Organosilica in Poly(ethylene oxide)

Kim, Youngdo,Kwon, Suk Jin,Jang, Hye-kyeong,Jung, Byung Mun,Lee, Sang Bok,Choi, U Hyeok American Chemical Society 2017 Chemistry of materials Vol.29 No.10

<P>A novel mesoporous silica-based single-ion conductor for lithium-ion batteries was prepared via two-step selective functionalization of designated silica precursors into the inner pore wall of mesoporous silica. 2-[(Trifluoromethanesulfonylimido)-N-4-sulfonylphenyl]ethyl (TFSISPE) group was first incorporated as a silica precursor having an anionic weak-binding imide group, and a dense brush of oligo-poly(ethylene glycol) (oligo-PEG) moieties, solvating Li, was cografted to produce functionalized mesoporous silica (FMS-TFSISPE) nanoparticles. FMS-TFSISPE showed a 2D hexagonal nanopore structure and a regular spherical shape with an average diameter of 50 nm. Poly(ethylene oxide) (PEO) was used to form a dispersion of the mesoporous silica nanoparticles into the polymer matrix. This new polymer-mesoporous silica nanohybrid solid electrolyte with the sole mobile Li ions (FMS-TFSISPE-PEO) exhibits attractive electrical, mechanical, and electrochemical properties. The ionic conductivity and storage modulus both increase simultaneously upon addition of FMS-TFSISPE nanoparticles. A 30 wt % amount of FMS-TFSISPE nanop articles leads to the highest ionic conductivity (sigma(DC) similar to 10(-3) S/cm at 25 degrees C) and storage modulus (G' similar to 10(4) Pa at 30 degrees C) with a high lithium ion transference number (t(Li)(+) similar to 0.9). Compared to conventional nonporous silica nanoparticles-incorporated PEO matrix (SiO2-TFSISPE-PEO), FMS-TFSISPE-PEO exhibits 2 orders of magnitude higher ionic conductivity with lower activation energy, suggesting that the facile transportation of lithium ions is achieved through the continuous weak-binding and solvating nanopore channel of the mesoporous silica retaining a high surface area and pore volume.</P>