진화프로그래밍 분류 기법을 이용한 이상 유전자 진단 시스템 제안

김영지,송병호,배상현 朝鮮大學校 統計硏究所 2007 統計硏究所論文誌 Vol.9 No.1

DNA기술의 발달로 대량으로 얻어진 유전자 정보를 Microarray 기술을 이용하여 손쉽게 이상 값을 가진 유전자의 정확한 분류와 진단을 할 수 있을 것이란 기대가 커지고 있다. 정확한 분류를 하기 위해서는 추출된 유전자에 들어 있는 많은 잡음 즉 이상 값이 들어있기 때문에 정상 값과 다른 이상 값을 가진 유전자만을 추출할 필요가 있다. 따라서 본 논문에서는 여러 가지 유전자 추출방법과 세 가지 dataset에 대하여 조사하여 보았다. 그리고 제안한 메타-진화프로그래밍을 사용한 분류 방법이 보다 효과적일 것이라고 예측해보았다.

여러부식용액에서 CR-39 비적 검출기의 특성에 관한 연구

김현구,김영기 조선대학교 기초과학연구소 1989 自然科學硏究 Vol.12 No.1

The characteristics of SSNTD, for the sensitibility of the detector, depending on etching conditnot, can be well understood, if its optimum etching condition, track etch rate V_T and vulk etch rate V_B are known. In this study the etching characteristics and etching condition of the CR-39 plastic detector have been studied using KOH and NaOH, with the addition of KSCN, NaSCN, Bisphenol and an alcohol solvent. Values of the activation energy E in KOH and NaOH are 0.74±0.02eV and 0.76±0.01eV From measurement of track etch rate V_T and etch rate ratio V(=V_T/V_B), the optimum etching condition can be obtained. The CR-39 detector was irradiated with a 0.12μCi Americium-241 alpha source.

수질환경에서 일어나는 항생물질 내성유전자의 전이와 재조합

김치경, 이성기, 김영창 忠北大學校 遺傳工學硏究所 1987 遺傳工學硏究 Vol.1 No.1

본문참고

Hexestrol의 投與가 不垂體剔出 흰쥐의 子宮에 미치는 影響

田暢淇,金榮默 충남대학교 대학원 1975 論文集 Vol.5 No.-

In order to find out the functional relationship between ovary and uterus not related to other endocrine glands in albino rats, weight of uterus, height of endometrial epithelium, width of muscular layer and histological change were investigated in hypophysectmized rats after ad mininistration of hexestrol (6.0 ㎎/rat) during ranging 1-56 days, and the results obtained are as follows. 1. The weight of uterus was decreased gradually in hypophysectomized group without treatment of hexestrol but was increased in hexestrol treatment group until 14 days and decreased thereafter. The significance between both group was highly recognized. 2. The height of endometrial epithelium, width of circular and longitudinal muscle layer of uterus showed high significance as compared with control group. Those value in hypophysectmized group was decreased as the time passed and in hexestrol treatment group showed the peak at 14th day. 3. The tissue of uterus was reacted sensitively with hexestrol and the change of hyperlasia was recognized clearly but hypophysectomized group showed the change of degeneration. 4. It was showed that uterus was only controlled by ovary but other endocrine glands were not directly affected on uterus.

Hepatocellular carcinoma in old age: are there any benefits of liver resection in old age?

신인식,Deok Gie Kim,Sung Whan Cha,Seong Hee Kang,Sung Hoon Kim,Moon Young Kim,Soon Koo Baik 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.2

Purpose: Elderly individuals have comorbidities that can adversely affect surgical outcomes. Some studies reported that elderly patients with hepatocellular carcinoma (HCC) have higher liver- and non-liver–related deaths. Therefore, palliative treatments are preferred in these patients. We compared surgical treatment outcomes between young and old age groups. Methods: In total, 233 liver resections were performed in patients with HCC from March 2012 to December 2018. We retrospectively reviewed medical records. The old age group was defined as patients aged more than 70 years. We compared perioperative characteristics and surgical outcomes and analyzed the prognostic factors for disease-free survival (DFS) and overall survival (OS) rates. Results: The young and old age group included 184 and 49 patients, respectively. Preoperative characteristics were similar. Major liver resection rate was similar (young age group, 26.1% vs. old age group, 20.4%), but the operation time was a little bit shorter in old age group. Major postoperative complications were 23 (12.5%) and 9 (18.4%) in the young and old age group (P = 0.351). Median non-liver–related overall survival were 80 and 76 months (P = 0.889) and liver-related OS were 76 and 76 months (P = 0.514) in the young and old age groups, respectively. Age was not an independent risk factor for DFS and OS. Conclusion: Elderly patients showed similar non-liver- and liver-related OS rates as young patients after liver resection. Postoperative complications were also similar. If elderly patients are well selected, they can receive curative treatment and show good surgical outcomes.

CDK7 is a reliable prognostic factor and novel therapeutic target in epithelial ovarian cancer

Kim, Jihye,Cho, Young-Jae,Ryu, Ji-Yoon,Hwang, Ilseon,Han, Hee Dong,Ahn, Hyung Jun,Kim, Woo Young,Cho, Hanbyoul,Chung, Joon-Yong,Hewitt, Stephen M.,Kim, Jae-Hoon,Kim, Byoung-Gie,Bae, Duk-Soo,Choi, Chel Elsevier 2020 Gynecologic oncology Vol.156 No.1

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>Cyclin-dependent kinase 7 (CDK7) engages tumor growth by acting as a direct link between the regulation of transcription and the cell cycle. Here, we investigated the clinical significance of CDK7 expression and its potential as a therapeutic target in epithelial ovarian cancer (EOC).</P> <P><B>Methods</B></P> <P>CDK7 expression was examined in 436 ovarian tissues including normal to metastatic ovarian tumors using immunohistochemistry, and its clinical implications were analyzed. Furthermore, we performed <I>in vitro</I> and <I>in vivo</I> experiments using CDK7 siRNA or a covalent CDK7 inhibitor (THZ1) to elucidate the effect of CDK7 inhibition on tumorigenesis in EOC cells.</P> <P><B>Results</B></P> <P>The patient incidence of high CDK7 expression (CDK7<SUP>High</SUP>) gradually increased from normal ovarian epithelium to EOC (<I>P</I> < 0.001). Moreover, CDK7<SUP>High</SUP> was associated with an advanced stage and high-grade histology (<I>P</I> = 0.035 and <I>P</I> = 0.011, respectively) in EOC patients and had an independent prognostic significance in EOC recurrence (<I>P</I> = 0.034). CDK7 inhibition with siRNA or THZ1 decreased cell proliferation and migration, and increased apoptosis in EOC cells, and this anti-cancer mechanism is caused by G0/G1 cell cycle arrest. In <I>in vivo</I> therapeutic experiments using cell-line xenograft and PDX models, CDK7 inhibition significantly decreased the tumor weight, which was mediated by cell proliferation and apoptosis.</P> <P><B>Conclusion</B></P> <P>Mechanistic interrogation of CDK7 revealed that it is significantly associated with an aggressive phenotype of EOC, and it has independent prognostic power for EOC recurrence. Furthermore, CDK7 may be a potential therapeutic target for patients with EOC, whether platinum sensitive or resistant.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CDK7 inhibition has an anti-cancer effect on platinum-sensitive EOC. </LI> <LI> CDK7 inhibition could induce responsiveness to platinum chemotherapy in platinum-resistant EOC. </LI> <LI> CDK7 overexpression had independent negative prognostic value for disease recurrence of EOC. </LI> <LI> CDK7 might play a critical role in EOC tumorigenesis, and it also serves as a possible therapeutic target in EOC. </LI> </UL> </P>

GO-56 : Anti-cancer effect of Linalool in Epithelial Ovarian Cancer

( Young Jae Cho ),( Jung Joo Choi ),( Yoo Young Lee ),( Chel Hun Choi ),( Tae Joong Kim ),( Jeong Won Lee,),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: Cancer chemotherapy has been applied to advanced tumors and intractable tumors, playing an important role in their treatments. However, there are many problems including the appearance of adverse reactions and acquirement of drug resistance. The increased numbers of medications resulted in a decreased quality of life for the patients. In the present study, the effects of linalool, a monoterpene alcohol found in the essential oils from many aromatic plants, on growth of ovarian cancer cell lines, were investigated. 방법: The effects of linalool on cell proliferation, apoptosis in ovarian cancer cells (HeyA8, HeyA8-MDR, A2780par, A2780cp-20, 105 cells/0.2 ´SKOV3ip-1, SKOV3-TR) in vitro were examined. In vivo, HeyA8 (2.5 106 cells/0.2 mL HBSS) were injected i.p. into the´mL HBSS) or SKOV3ip1 (1.0 peritoneal cavity of mice and linalool (300mg/kg, 600mg/kg/three times a week X9days, i.p.) was administered. The tumor was collected and weighed. 결과: Linalool inhibits cell proliferation in ovarian cancer cells, wild type and resistant type cells, and it induced apoptosis effects associated with activation of caspase 8 and 9. In addition, linalool led to increasing in reactive oxygen species (ROS) generation. In both HeyA8 and A2780par models in vivo, the mice in the linalool groups had significantly decreased tumor weight (both p< 0.05) compared with control(PBS). 결론: The present study indicates linalool has anti-proliferation and apoptotic effects in ovarian cancer cells. These results suggest that linalool may represent a novel class of lead compound for developing potential therapeutic agents for ovarian cancer.

GO-57 : Patient-derived xenograft models identify HER2 as a therapeutic target in HER2-amplified cervical cancer

( Young Jae Cho ),( Jung Joo Choi ),( Yoo Young Lee ),( Chel Hun Choi ),( Tae Joong Kim ),( Jeong Won Lee ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: Cervical cancer shows high incidence and frequent recurrence. However, effective chemotherapy options for cervical cancer are exceedingly limited. Therefore, targeted therapeutics for treatment of cervical cancer is needed. 방법: Nine fresh surgical samples from patients with cervical cancer were taken. The tumor tissues were injected to the subrenal capsule of immunodeficient mice. Serially passaged tumors retained the genetic features of original counterparts. Histologic examination, short tandem repeat analysis and array comparative genomic hybridization were done to evaluate successful establishment. One patient-xenograft pair showing HER2 amplification was further investigated by PCR, in situ hybridization and western blotting. 결과: Eight squamous cell carcinomas and one adenocarcinoma from patient were implanted to mice. Histology, STR, aCGH revealed homology between the pairs. We found that CX17 had HER2 amplification whose level was increased by serial passage. HER2 immunohistochemistry revealed cytoplasmic staining in CX17 primary tissue, however, cytoplasmic HER2 was changed into membranous HER2 during serial passage, suggesting that selection of HER2 is amplified in serially passaged tumors 결론: We established in vivo xenograft models from cervical cancer patients which is homologous in molecular and histologic aspects. Also, we suggest novel HER2 inhibitors-based therapeutic opportunities in HER2 amplified cervical patients.

GO-24 : Induction of autophagy increase chemosensitivity of drug-resistant ovarian cancer

( Young Ae Park ),( Jeong Won Lee ),( Chel Hun Choi ),( Yoo Young Lee ),( Jung Joo Choi ),( Tae Joong Kim ),( Hye Kyung Jeon,),( Young Jae Cho ),( Ji Yoon Ryu ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

Drug resistance is one of major obstacle that reduce the effectiveness of Drug resistance is one of major obstacle that reduce the effectiveness of chemotherapy of ovarian cancer, via requiring novel therapeutic strategies that could induce the response to the anti cancer reagents. Recently, the induction of autophagy has been suggested as a tool that could overcome the drug resistance of cancer cells. Combined treatment of autophagy activator with some drugs induced chemo sensitivities by reducing cell viability or inducing apoptosis in several cancer types, particularly those which are platinum-based drugs. However, there are not yet results that clearly show the ability of autophagy to overcome of drug resistance of ovarian cancer cells. The aim of this study is to estimate the therapeutic effects of the regulation of autophagy on the drug resistant ovarian cancer cells. We estimated the expression of LC3-2 and p62, defined components of autophagy mechanism in drug resistant ovarian cancer cell lines and compared them with those in individual parent cell lines (drug sensitive cell lines). We pretreated autophagy regulator, chloroquine (CQ) at ovarian cancer cells and cells were incubated with cisplatin. Combined treatment of them mediated alteration of cell viabilities were estimated by MTT assay and the change of autophagy was through estimation of protein expression levels of them by Western blotting assay using specific antibody against them. We found that both LC3-2 and p62 were relatively down-regulated in drug resistant ovarian cancer cells. Pretreatment of CQ increased the response to the cisplatin and bafilomycin induced reverse results. Moreover, we identified that single treatment of CQ reduced cell viability and combined treatment of CQ and cisplatin increased the expression of both LC3-2 and p62 more efficiently than those by single treatment of either cisplatin or CQ in cisplatin resistant ovarian cancer cells. These results indicate the potential therapeutic strategy of the regulation of autophagy in ovarian cancer cells. Drug resistant ovarian cancer cells exhibited reduced expression of components of autophagy in comparison to their parent cells and combined treatment of autophagy regulator with cisplatin modified response to the cisplatin via reducing cell viability. Single treatment of cisplatin increased autophagy and increased cytotoxic effects when treated with autophagy activator. Therefore, our results show that induction of autophagy could be novel therapeutic strategy to overcome drug resistance of ovarian cancer cells.

A New Localization System for Mobile Robots Using Radio Frequency and Ultrasound

Young-gie Kim,Jong-Suk Choi,Jin-Oh Kim,Munsang Kim 제어로봇시스템학회 2002 제어로봇시스템학회 국제학술대회 논문집 Vol.2002 No.10