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Production of cellulases and β-glucosidase in Trichoderma reesei mutated by proton beam irradiation
You Ree Jung,김승욱,Hyun Yong Shin,Hah Young Yoo,엄영순 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.7
To obtain mutant strains producing high levels of cellulases (FPase and CMCase) and β-glucosidase, Trichoderma reesei KCTC 6950 was mutated by proton beam irradiation. Five mutants were selected out of 1,000 mutants of T.reesei treated with proton beam irradiation, based on their ability for enzyme production on a plate screening medium. In submerged cultures containing Mandel’s fermentation medium, the mutant strain T-2 (MT-2) demonstrated a 165% increase in the activity of FPase, a 146% increase in the activity of CMCase, and a 313% increase in the activity of β-glucosidase, compared with the wild type strain. Additionally, the properties of high level β-glucosidase produced by MT-2 were the same as those of the wild type strain, e.g., an optimum pH of 4.8, and an optimum temperature of 65 oC. Moreover, the protein concentrations of β-glucosidase produced by the wild type strain and MT-2 were measured by SDS-PAGE, and then β-glucosidase activities were detected by the MUG-zymogram assay.
You Ree Jung,Hyun Yong Shin,송윤석,Sung Bong Kim,김승욱 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.2
In this study, b-glucosidase from Aspergillus niger was pretreated with cellobiose and glucose to prevent loss of enzyme activity, and pretreated b-glucosidase was immobilized on silica gel as a carrier by covalent binding. To enhance the activity of immobilized b-glucosidase, the effects of substrate concentration and reaction conditions, including temperature, time, and agitation speed, were investigated. The optimal concentrations of cellobiose and glucose, temperature, time, and agitation speed were determined to be 0.02 M, 40 8C, 20 min, and 130 rpm, respectively. The activity of immobilized b-glucosidase after pretreatment was increased to about 176% of that of non-pretreated bglucosidase. In addition, the optimal pH and temperature of the non-pretreated and pretreated immobilized b-glucosidases were both pH 5.5 and 65 8C, respectively. Moreover, the immobilized bglucosidases were used repeatedly 20 times, and the enzyme activities were maintained at levels higher than 80% of their initial activities.
Jung, You Ree,Shin, Hyun Yong,Song, Yoon Seok,Kim, Sung Bong,Kim, Seung Wook Elsevier 2012 Journal of industrial and engineering chemistry Vol.18 No.2
<P><B>Abstract</B></P><P>In this study, <I>β</I>-glucosidase from <I>Aspergillus niger</I> was pretreated with cellobiose and glucose to prevent loss of enzyme activity, and pretreated <I>β</I>-glucosidase was immobilized on silica gel as a carrier by covalent binding. To enhance the activity of immobilized <I>β</I>-glucosidase, the effects of substrate concentration and reaction conditions, including temperature, time, and agitation speed, were investigated. The optimal concentrations of cellobiose and glucose, temperature, time, and agitation speed were determined to be 0.02M, 40°C, 20min, and 130rpm, respectively. The activity of immobilized <I>β</I>-glucosidase after pretreatment was increased to about 176% of that of non-pretreated <I>β</I>-glucosidase. In addition, the optimal pH and temperature of the non-pretreated and pretreated immobilized <I>β</I>-glucosidases were both pH 5.5 and 65°C, respectively. Moreover, the immobilized <I>β</I>-glucosidases were used repeatedly 20 times, and the enzyme activities were maintained at levels higher than 80% of their initial activities.</P>
Inhibition of BET selectively eliminates undifferentiated pluripotent stem cells
Im, Jung Hyun,Hwang, Seon In,Kim, Jong-Wan,Park, Soon-Jung,Kang, Kyu-ree,You, Jueng Soo,Kim, Kee Pyo,Moon, Sung-Hwan,Cha, Hyuk-Jin,Chung, Hyung-Min,Schö,ler, Hans R.,Hyun, Jung Keun,Han, Dong Wook Elsevier 2018 Science bulletin Vol.63 No.8
( You Ree Kim ),( Hae Woon Jung ) 경희대학교 경희의료원 2020 慶熙醫學 Vol.35 No.1
Precocious puberty is a disorder unique to children in which secondary sex characteristics develop before the age of 8 years in girls and 9 years in boys. The incidence of precocious puberty in Korea has substantially increased in recent years, especially among girls. Most of these cases are due to idiopathic central precocious puberty, whereas peripheral precocious puberty occurs infrequently. We experienced a case of a young girl with peripheral precocious puberty due to an autonomous ovarian cyst whose symptoms and signs of precocity occurred after frequent application of placental cream to the face. The diagnostic process is explained and possible association of the ovarian cyst to placental cream exposure is explored. The case highlights the clinical course and management of peripheral precocious puberty due to an autonomous ovarian cyst and emphasizes caution about the use of placental cream in children.