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The elastoplastic formulation of polygonal element method based on triangular finite meshes
Cai, Yong-Chang,Zhu, He-Hua,Guo, Sheng-Yong Techno-Press 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.30 No.1
A small strain and elastoplastic formulation of Polygonal Element Method (PEM) is developed for efficient analysis of elastoplastic solids. In this work, the polygonal elements are constructed based on traditional triangular finite meshes. The construction method of polygonal mesh can directly utilize the sophisticated triangularization algorithm and reduce the difficulty in generating polygonal elements. The Wachspress rational finite element basis function is used to construct the approximations of polygonal elements. The incremental variational form and a von Mises type model are used for non-linear elastoplastic analysis. Several small strain elastoplastic numerical examples are presented to verify the advantages and the accuracy of the numerical formulation.
The elastoplastic formulation of polygonal element method based on triangular finite meshes
Yong-chang Cai,He-hua Zhu,Sheng-yong Guo 국제구조공학회 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.30 No.1
A small strain and elastoplastic formulation of Polygonal Element Method (PEM) is developed for efficient analysis of elastoplastic solids. In this work, the polygonal elements are constructed based on traditional triangular finite meshes. The construction method of polygonal mesh can directly utilize the sophisticated triangularization algorithm and reduce the difficulty in generating polygonal elements. The Wachspress rational finite element basis function is used to construct the approximations of polygonal elements. The incremental variational form and a von Mises type model are used for non-linear elastoplastic analysis. Several small strain elastoplastic numerical examples are presented to verify the advantages and the accuracy of the numerical formulation.
Conjugation of Homing Peptide to Magnetite Nanoparticles and Their Specific Intracellular Uptake
( Sun Jung Kim ),( Zhi Cai Xing ),( Moon Jeong Choi ),( Seung Jin Han ),( Byung Heon Lee ),( Yong Min Chang ),( Inn Kyu Kang ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.2
In this study, a homing peptide denoted as AP was successfully conjugated to the surface of aminodextrancoated magnetite nanoparticles (ADNP) in order to use it as a targeting ligand for IL-4 receptors bearing tumor cells. Fluorescence isothiocyanate (FITC) was also immobilized on the AP-conjugated magnetite nanoparticles (PDNP) to visualize the nanoparticles in the cells. The PDNP were monodispersed and superparamagnetic in nature at room temperature. It was revealed, from cell culture experiments, that the PDNP are biocompatible. Again, microscopic fluorescence images showed that FITC-bound PDNP (FPDNP) were specifically internalized into HT1376 cells and emitted intense fluorescence images whereas FITC-bound DNP (FDNP) were slightly internalized into the cells. Furthermore, confocal images confirmed that the fluorescence signal originates from the cells interior. These phenomena indicate that AP-conjugated magnetite nanoparticles have a high affinity to IL-4 receptor-bearing tumor cells. Thus, AP-conjugated magnetite nanoparticles have the potential to be used for molecular imaging and other biomedical applications.
Hwang, Eung-Soo,Zhang, Zhigang,Cai, Haobin,Huang, David Y.,Huong, Shu-Mei,Cha, Chang-Yong,Huang, Eng-Shang American Society for Microbiology 2009 Journal of virology Vol.83 No.23
<B>ABSTRACT</B><P>Infection of host cells with human cytomegalovirus (HCMV) induces cell cycle dysregulation. Two HCMV immediate-early (IE) proteins, IE1-72 and IE2-86, are promiscuous transactivators that have been implicated in the dysregulatory events. Cellular p53 protein is accumulated to high levels in HCMV-infected cells, but the indicative marker of p53 transcriptional activity, p21, is markedly decreased. Both IE1-72 and IE2-86 were able to transactivate the p53 promoter and interact with p53 protein in DNA-transfected or HCMV-infected cells. HCMV UL84, a multiregulatory protein expressed in early periods of HCMV infection, also interacted with p53. HCMV IE1-72 prevented or disrupted p53 binding to p53-specific DNA sequences, while IE2-86 and/or UL84 enhanced p53 binding and induced supershift of this DNA-protein complex. Both HCMV IE1-72 and IE2-86 were able to inhibit p53-dependent transcriptional activation in plasmid-transfected cells. IE1-72, rather than IE2-86, was found to be responsible for p21 downregulation in HCMV-infected HEL cells. DNA transfection analysis using IE1-72 mutants revealed that exon 2/3 and the zinc finger region of IE1-72 are essential for IE1-72's effect on the repression of p53-dependent transcriptional activation. These data suggest that HCMV IE1-72 and/or IE2-86 transactivates the p53 promoter and induces p53 accumulation, but HCMV IE1-72 represses the p53 transactivation activity by a unique binding hindrance mechanism different from that of IE2-86. Thus, various modes of viral IE proteins and p53 interactions might result in multiple outcomes, such as stimulation of cellular DNA synthesis, cell cycle progression and cell cycle arrest, and prevention of program cell death.</P>
Long non-coding RNAs in Sus scrofa ileum under starvation stress
Wang Shu,Ma Yi Jia,Li Yong Shi,Ge Xu Sheng,Lu Chang,Cai Chunbo,Yang Yang,Zhao Yan,Liang Guo Ming,Guo Xiaohong,Cao Guoqing,Li Bugao,Gao Pengfei 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.7
Objective: In this study, we aimed to identify long non-coding RNAs (lncRNAs) that play important roles in starvation stress, analyze their functions, and discover potential molecular targets to alleviate starvation stress to provide a theoretical reference for subsequent in-depth research. Methods: We generated a piglet starvation stress animal model. Nine Yorkshire weaned piglets were randomly divided into a long-term starvation stress group (starved for 72 h), short-term starvation stress group (starved for 48 h), and the control group. LncRNA libraries were constructed using high-throughput sequencing of piglet ileums. Results: We obtained 11,792 lncRNAs, among which, 2,500 lncRNAs were novel. In total, 509 differentially expressed (DE)lncRNAs were identified in this study. Target genes of DElncRNAs were predicted via cis and trans interactions, and functional and pathway analyses were performed. Gene ontology functions and Kyoto encyclopedia of genes and genomes analysis revealed that lncRNA-targeted genes mainly participated in metabolic pathways, cellular processes, immune system processes, digestive systems, and transport activities. To reveal the mechanism underlying starvation stress, the interaction network between lncRNAs and their targets was constructed based on 26 DElncRNAs and 72 DEmRNAs. We performed an interaction network analysis of 121 DElncRNA–DEmRNA pairs with a Pearson correlation coefficient greater than 0.99. Conclusion: We found that MSTRG.19894.13, MSTRG.16726.3, and MSTRG.12176.1 might play important roles in starvation stress. This study not only generated a library of enriched lncRNAs in piglets, but its outcomes also provide a strong foundation to screen key lncRNAs involved in starvation stress and a reference for subsequent in-depth research. Objective: In this study, we aimed to identify long non-coding RNAs (lncRNAs) that play important roles in starvation stress, analyze their functions, and discover potential molecular targets to alleviate starvation stress to provide a theoretical reference for subsequent in-depth research.Methods: We generated a piglet starvation stress animal model. Nine Yorkshire weaned piglets were randomly divided into a long-term starvation stress group (starved for 72 h), short-term starvation stress group (starved for 48 h), and the control group. LncRNA libraries were constructed using high-throughput sequencing of piglet ileums.Results: We obtained 11,792 lncRNAs, among which, 2,500 lncRNAs were novel. In total, 509 differentially expressed (DE)lncRNAs were identified in this study. Target genes of DElncRNAs were predicted via cis and trans interactions, and functional and pathway analyses were performed. Gene ontology functions and Kyoto encyclopedia of genes and genomes analysis revealed that lncRNA-targeted genes mainly participated in metabolic pathways, cellular processes, immune system processes, digestive systems, and transport activities. To reveal the mechanism underlying starvation stress, the interaction network between lncRNAs and their targets was constructed based on 26 DElncRNAs and 72 DEmRNAs. We performed an interaction network analysis of 121 DElncRNA–DEmRNA pairs with a Pearson correlation coefficient greater than 0.99.Conclusion: We found that MSTRG.19894.13, MSTRG.16726.3, and MSTRG.12176.1 might play important roles in starvation stress. This study not only generated a library of enriched lncRNAs in piglets, but its outcomes also provide a strong foundation to screen key lncRNAs involved in starvation stress and a reference for subsequent in-depth research.
Wang Rongxi,Yu Xiaoyue,Wang Zhiqiang,Liu Yujie,Chen Hui,Liu Shangbin,XU CHENG,Chen Yingjie,Xia Danni,Ge Xin,Chang Ruijie,Xu Gang,Xiang Mi,Wang Ying,Shen Tian,Hu Fan,Cai Yong 한국역학회 2022 Epidemiology and Health Vol.44 No.-
OBJECTIVES: Proper blood lipid levels are essential for survival in older adults, but inconsistent relationships have been reported between blood lipids and all-cause mortality in the elderly. METHODS: This retrospective longitudinal study analyzed data from 1,067 Chinese older adults enrolled in the Chinese Longitudinal Healthy Longevity Survey collected in 2008 and followed up until death or December 31, 2018. The outcome was allcause mortality. Multivariate Cox regression analyses were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) with stratification by age (60-80, 80-100, or ≥ 100 years) for further analysis. The survival probability according to lipid profile quartiles was calculated using Kaplan-Meier curves and the log-rank test. RESULTS: The participants’ mean age was 84.84 years, and 57.0% were female. In total, 578 individuals died, and 277 were lost to follow-up. The mean total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were higher among those who died than among those who survived. Participants in the second HDL-C quartile and the highest LDL-C and triglyceride (TG) quartiles had 28% higher, 23% lower, and 49% lower risks of all-cause mortality, respectively. After further adjustment, the associations remained except for HDL-C, and additional associations were observed between all-cause mortality and the third TC and LDL-C quartiles and the second TG quartile (HR, 1.44; 95% CI, 1.01 to 2.06; HR, 0.68; 95% CI, 0.49 to 0.94; HR, 0.79; 95% CI, 0.62 to 0.99, respectively). CONCLUSIONS: Older adults should maintain an LDL-C level of 1.91-2.47 mmol/L and a TG level of no less than 1.66 mmol/L.