Differentially Expressed Genes under Cold Acclimation in Physcomitrella patens

( Ming Ming Sun ),( Lin Hui Li ),( Hua Xie ),( Rong Cai Ma ),( Yi Kun He ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.6

Differentially Expressed Genes under Cold Acclimation in Physcomitrella patens

Sun, Ming-Ming,Li, Lin-Hui,Xie, Hua,Ma, Rong-Cai,He, Yi-Kun Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6

Cold acclimation improves freezing tolerance in plants. In higher plants, many advances have been made toward identifying the signaling and regulatory pathways that direct the low-temperature stress response; however, similar insights have not yet been gained for simple nonvascular plants, such as bryophytes. To elucidate the pathways that regulate cold acclimation in bryophytes, we used two PCR-based differential screening techniques, cDNA amplified fragment length polymorphism (cDNA-AFLP) and suppression subtractive hybridization (SSH), to isolate 510 ESTs that are differentially expressed during cold acclimation in Physcomitrella patens. We used realtime RT-PCR to further analyze expression of 29 of these transcripts during cold acclimation. Our results show that cold acclimation in the bryophyte Physcomitrella patens is not only largely similar to higher plants but also displays distinct differences, suggests significant alteration during the evolution of land plants.

Pre-treatment Metabolic Tumor Volume and Total Lesion Glycolysis are Useful Prognostic Factors for Esophageal Squamous Cell Cancer Patients

Li, Yi-Min,Lin, Qin,Zhao, Long,Wang, Li-Chen,Sun, Long,Dai, Ming-Ming,Luo, Zuo-Ming,Zheng, Hua,Wu, Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Objectives: To study application of the maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV) and total lesion glycolysis (TLG) with $^{18}F$-FDG PET/CT for predicting prognosis of esophageal squamous cell cancer (ESC) patients. Methods: Eighty-six patients with ESC staged from I to IV were prospectively enrolled. Cisplatin-based chemoradiotherapy (CCRT) or palliative chemoradiotherapy were the main treatment methods and none received surgery. $^{18}F$-FDG PET/CT scans were performed before the treatment. SUVmax, MTV, and TLG were measured for the primary esophageal lesion and regional lymph nodes. Receiver operating characteristic curves (ROCs) were generated to calculate the P value of the predictive ability and the optimal threshold. Results: MTV and TLG proved to be good indexes in the prediction of outcome for the ESC patients. An MTV value of 15.6 ml and a TLG value of 183.5 were optimal threshold to predict the overall survival (OS). The areas under the curve (AUC) for MTV and TLG were 0.74 and 0.70, respectively. Kaplan-Meier analysis showed an MTV less than 15.6 ml and a TLG less than 183.5 to indicate good media survival time (p value <0.05). In the stage III-IV patient group, MTV could better predict the OS (P < 0.001), with a sensitivity and specificity of 0.80 and 0.67, respectively. Conclusions: Pre-treatment MTV and TLG are useful prognostic factors in nonsurgical ESC.

A Resonant Characteristics Analysis and Suppression Strategy for Multiple Parallel Grid-connected Inverters with LCL Filter

Sun, Jian-jun,Hu, Wei,Zhou, Hui,Jiang, Yi-ming,Zha, Xiao-ming The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.4

Multiple parallel inverters have multiple resonant frequencies that are influenced by many factors. This often results in stability and power quality problems. This paper develops a multiple input multiple output model of grid-connected inverter systems using a closed-loop transfer function. The influence factors of the resonant characteristics are analyzed with the developed model. The analysis results show that the resonant frequency is closely related to the number, type and composition ratio of the parallel inverters. To suppress resonance, a scheme based on virtual impedance is presented, where the virtual impedance is emulated in the vicinity of the resonance frequency. The proposed scheme needs one inverter with virtual impedance control, which reduces the design complexity of the other inverter controllers. Simulation and experimental tests are carried out on two single phase converter-based setups. The results validate the correctness of the model, the analytical results and the resonant suppressing scheme.

Exploring the Key Genes and Pathways of Osteoarthritis in Knee Cartilage in a Rat Model Using Gene Expression Profiling

Yi-Ming Ren,Xin Zhao,Tao Yang,Yuan-Hui Duan,Yun-Bo Sun,Wen-Jun Zhao,Meng-Qiang Tian 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.6

Purpose: To compare differentially expressed genes (DEGs) mediating osteoarthritis (OA) in knee cartilage and in normal kneecartilage in a rat model of OA and to identify their impact on molecular pathways associated with OA. Materials and Methods: A gene expression profile was downloaded from the Gene Expression Omnibus database. Analysis ofDEGs was carried out using GEO2R. Enrichment analyses were performed on the Gene Ontology (GO) and Kyoto Encyclopedia ofGenes and Genomes pathway using the Search Tool for the Retrieval of Interacting Genes database (http://www.string-db.org/). Subsequently, the regulatory interaction network of OA-associated genes was visualized using Cytoscape software (version 3.4.0;www.cytoscape.org). Results: In the gene expression profile GSE103416, a total of 99 DEGs were identified. Among them, 76 DEGs (76.77%) were overexpressed,and the remaining 23 DEGs (23.23%) were underexpressed. GO and pathway enrichment analyses of target geneswere performed. Using gene-gene interaction network analysis, relevant core genes, including MET, UBB, GNAI3, and GNA13,were shown to hold a potential relationship with the development of OA in cartilage. Using quantitative real-time PCR, the Gna13/cGMP-PKG signaling pathway was identified as a potential research target for therapy and for further understanding the developmentof OA. Conclusion: The results of the present study provide a comprehensive understanding of the roles of DEGs in knee cartilage in relationto the development of OA.

Interleukin 10 rs1800872 T>G Polymorphism was Associated with an Increased Risk of Esophageal Cancer in a Chinese Population

Sun, Jia-Ming,Li, Qiong,Gu, Hai-Yong,Chen, Yi-Jang,Wei, Ji-Shu,Zhu, Quan,Chen, Liang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Aim: Esophageal cancer is the eighth most common cancer and sixth leading cause of cancer associated death worldwide. The 5 year survival rate for esophageal cancer patients is very poor and accounts for only 12.3%. Besides environmental risk factors, genetic factors might play an important role in the esophageal cancer carcinogenesis. Methods: We conducted a hospital based case-control study to evaluate the genetic effects of functional single nucleotide polymorphisms (SNPs): interleukin 9 (IL9) rs31563 C>T, IL9 rs31564 G>T, IL10 rs1800872 T>G, IL12A rs2243115 T>G, IL12B rs3212227 T>G and IL13 rs1800925 C>T on the development of esophageal cancer. A total of 380 esophageal squamous cell carcinoma (ESCC) cases and 380 controls were recruited for this study. The genotypes were determined using a custom-by-design 48-Plex SNPscan$^{TM}$ Kit. Results: The IL10 rs1800872 T>G polymorphism was associated with an increased risk of ESCC. However, there were no significant links with the other five SNPs. Stratified analyses indicated no significant risk of ESCC associated with the IL10 rs1800872 T>G polymorphism evident among any subgroups. Conclusion: These findings indicated that functional polymorphism IL10 rs1800872 T>G might contribute to ESCC susceptibility. However, our results were obtained with a limited sample size, so that the power of our analysis was low. Future larger studies with more rigorous study designs of other ethnic populations are required to confirm the current findings.

중국 윈난성 뎬츠(滇池)보호에서의 공중참여에 관한 연구 : 자원동원이론의 시각으로

Yi-Hua Wen,Yue Sun,Ming Liu 한국환경사회학회 2019 환경사회학연구 ECO Vol.23 No.2

This research takes the private environment organization--Green Kunming as an example, to explore the growth of resources by mobilization. The study includes a public participation activity--Protecting Lake Denz, the largest lake in Kunming, Yunnan Province. To ensure this organization survive and thrive, we must have sufficient resources and are able to mobilize them. This study focuses on the development of Green Kunming ancestry conservation activities based on institutional resources, financial resources, human resources and social resources. These resources are organically integrated with each other and are characterized by collective actions. The survey results show that Green Kunming is limited by institutional resources, but this is also a prerequisite for obtaining legitimacy and obtaining new resources. Financial resources are mainly funded by private fund meetings. and these funds are a major participatory survival method including projects. In mobilizing human resources, the members are mainly volunteers. Doing this will ensure the sustainability of group development, promote the formation of social capital, and fully mobilize new media as a social resource. This study aims to determine the growth of citizens in our society, and hope to use it as an indicator to measure the degree of the political structure openness to the Chinese public participation and the degree of democratization in the public sphere. 본 연구는 중국 윈난성 쿤밍시에서 가장 큰 호수인 뎬츠(滇池)에 대한 보호를 비롯한 공중의 참여활동 중에서 민간 환경단체-녹색곤명을 사례로, 자원동원론의 시각으로 참여과정에 자원을 어떻게 동원하면서 성장하였는가를 살펴본다. 단체가 생존하고 성장하려면 다양한 자원을 소유함과 아울러 자원을 충분히 동원하여야 한다. 이런 자원들은 또 서로 유기적으로 결합되면서 단체활동 전개의 특성을 가진다. 본고에서는 자원을 동원하기 위한 전제조건, 재정적 자원, 인적 자원, 사회적 자원을 토대로 뎬츠보호의 주요한 행위주체‒녹색곤명의 뎬츠보호 활동의 전개에 대한 분석이 중심을 이룬다. 연구결과 녹색곤명은 합법적인 지위를 취득하여 새로운 자원을 얻을 수 있는 정치적 기회구조를 확충하고, 재정적 자원은 주로 민간 기금회의 자금 지원을 통해 얻는데 이러한 자금조달은 프로젝트를 비롯한 참여방식이 된다. 인적 자원의 동원을 볼때 구성원은 주로 자원봉사자들인데 이러한 특성은 인력동원이 용이하고 자발적으로 활동할 수 있으며 단체 발전의 지속성을 보장하며, 사회자본의 형성을촉진할 수 있다. 사회적 자원으로는 주로 뉴미디어를 활용했는데 전파속도가빠르고 전파범위가 넓어서 공론장의 형성을 촉진하였다. 이러한 현상을 통해중국에서 공중참여에 대한 제도적 보장은 이미 갖춰져 있고, 정치적 기회 구조는 질적으로 변화하고 있으며, 정부의 집권이념의 변화가 시민사회의 성장을추진하고 있다는 결론을 얻을 수 있었다.

UPLC-Q-TOF-MS/MS Analysis for Steaming Times-dependent Profiling of Steamed Panax quinquefolius and Its Ginsenosides Transformations Induced by Repetitious Steaming

Sun, Bai-Shen,Xu, Ming-Yang,Li, Zheng,Wang, Yi-Bo,Sung, Chang-Keun The Korean Society of Ginseng 2012 Journal of Ginseng Research Vol.36 No.3

The metabolic profiles of Panax quinquefolius and its associated therapeutic values are critically affected by the repetitious steaming times. The times-dependent steaming effect of P. quinquefolius is not well-characterized and there is also no official guideline on its times of steaming. In this paper, a UPLC-Q-TOF-MS/MS method was developed for the qualitative profiling of multi-parametric metabolic changes of raw P. quinquefolius during the repetitious steaming process. Our method was successful in discriminating the differentially multi-steamed herbs. Meantime, the repetitious steaming-inducing chemical transformations in the preparation of black American ginseng (American ginseng that was subjected to 9 cycles of steaming treatment) were evaluated by this UPLC-Q-TOF-MS/MS based chemical profiling method. Under the optimized UPLC-Q-TOF-MS/MS conditions, 29 major ginsenosides were unambiguously identified and/or tentatively assigned in both raw and multi-steamed P. quinquefolius within 19 min, among them 18 ginsenosides were detected to be newly generated during the preparatory process of black American ginseng. The mechanisms involved were further deduced to be hydrolysis, dehydration, decarboxylation and addition reactions of the original ginsenosides in raw P. quinquefolius through analyzing mimic 9 cycles of steaming extracts of 14 pure reference ginsenosides. Our novel steaming times-dependent metabolic profiling approach represents the paradigm shift in the global quality control of multi-steamed P. quinquefolius products.

APPROACH TO CONTROL METHOD OF PLANNING LEVEL FOR EXCAVATING ROBOT

Sun Shou Qian,Xu Hai Ming,Wang Yi Jun,He Bao Liang 대한전자공학회 1992 대한전자공학회 Conference Vol.1992 No.2

Proteomic Changes in Rice Root and Shoot under Nitrogen, Phosphate, and Potassium Starvation

Yi Ming Wang,Sun Tae Kim,Sang Gon Kim,Chang Hoon Lee,Pil Joo Kim,Kyu Young Kang 한국응용생명화학회 2008 Applied Biological Chemistry (Appl Biol Chem) Vol.51 No.5