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고려 엉겅퀴(Cirsium setidens Nakai) 잎 조직을 이용한 callus 배양 및 항산화 활성 검증
박정훈,심예지,박기임,이인순,문혜연 대구대학교 특수교육재활과학연구소 2010 産業技術硏究 Vol.21 No.1
We described the effects of antioxidant activity on the extracts of tissue and callus formation by Cirsium setidens Nakai. The callus culture were induced from leaf tissue of Cirsium setidens in both MS and B5 medium with IAA or/and kinetin (0∼5.0 ㎎/ℓ). As medium for callus culture induced from Cirsium setidens, B5 medium showed better condition than MS media. MS medium brought to decrease callus differentiation after formation, on the other hand B5 medium induced to accelerate callus differentiation. Among phytohormones is required 0.5 ㎎/ℓ of IAA and between 2.0 ㎎/ℓ and 1.0 ㎎/ℓ of Kinetin for formation of callus. In order to extract functional material from natural leaf and callus tissue of Cirsium setidens used distilled water and 75% ethanol as solvent and those extract used to determine antioxidant activity as alpha-diphenyl-2-picryl-hydrozyl (DPPH) radicals scavenging effect and TBA(thiobarbituric acid) activity. In result, the effect of DPPH and TBA had high activity more natural leaf extract than callus tissue and 75% ethanol was solvent better than distilled water. The 75% ethanol extract of natural leaf showed an excellent antioxidant activity because that particularly had highest concentration of vitamin C and total phenol among those extracts
Lee, Chung-Gi,Song, Ye Jin,Lee, Ki Bong,Mun, Sungyong Elsevier 2019 Journal of industrial and engineering chemistry Vol.80 No.-
<P><B>Abstract</B></P> <P>To maximize the industrial value of biologically produced 2,3-butanediol (BD), it is essential to separate BD into its stereoisomeric fractions, <I>meso</I>-BD and <I>racemic</I>-BD. To address such issue, a highly efficient BD-isomer separation process based on a simulated-moving-bed (SMB) mode was developed in this study. As a first step for this task, the competitive adsorption behaviors of <I>meso</I>-BD and <I>racemic</I>-BD on a properly selected adsorbent were investigated, and their relevant isotherm parameters were determined using inverse method. The determined isotherm parameters were then applied to the design of the BD-isomer separation process based on an SMB mode, which was carried out in such a way that both BD isomers could be separated with high purities and little losses in a continuous countercurrent mode. The performance of the designed SMB process for the targeted separation was experimentally investigated. It was confirmed from the experimental results that the BD-isomer separation SMB process, which was developed in this study for the first time, could be effective in the continuous-mode separation of <I>meso</I>-BD and <I>racemic</I>-BD with the purities higher than 99.9% and 97% respectively while maintaining their losses below 1%. The results of this study are expected to pave the way for establishing a highly economical process for a large-scale production of <I>meso</I>-BD and <I>racemic</I>-BD with high purities.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The SMB for continuous-mode separation of 2,3-butanediol (BD) isomers was developed. </LI> <LI> Highly effective adsorbent for separation of <I>meso</I>-BD and <I>racemic</I>-BD was found. </LI> <LI> Competitive Langmuir isotherm parameters of BD isomers were determined and validated. </LI> <LI> The resultant isotherm parameters were used to design the BD-isomer separation SMB. </LI> <LI> The designed SMB led to high purities of <I>meso</I>-BD and <I>racemic</I>-BD with little losses. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Ye-Gi,Jin, Yong-Su,Cha, Young-Lok,Seo, Jin-Ho Elsevier 2017 Bioresource technology Vol.228 No.-
<P><B>Abstract</B></P> <P>Even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. This study demonstrates that an industrial polyploidy <I>Saccharomyces cerevisiae</I> JHS200 can be engineered through Cas9 (CRISPR associated protein 9)-based genome editing. Specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into the auxotrophic mutants. As expected, the engineered strain (JX123) enhanced ethanol production from cellulosic hydrolysates as compared to other engineered haploid strains. However, the JX123 strain produced substantial amounts of xylitol as a by-product during xylose fermentation. Hypothesizing that the xylitol accumulation might be caused by intracellular redox imbalance from cofactor difference, the NADH oxidase from <I>Lactococcus lactis</I> was introduced into the JX123 strain. The resulting strain (JX123_noxE) not only produced more ethanol, but also produced xylitol less than the JX123 strain. These results suggest that industrial polyploidy yeast can be modified for producing biofuels and chemicals.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Industrial <I>Saccharomyces cerevisiae</I> was able to edit genome by CRISPR-Cas9. </LI> <LI> A xylose assimilating pathway was introduced into an industrial yeast strain. </LI> <LI> Expression of NADH oxidase improved the bioethanol productivity. </LI> <LI> The concentration of ethanol produced by silver grass hydrolysates is 55.51g/L. </LI> </UL> </P>
Acetate-rich Cellulosic Hydrolysates and Their Bioconversion Using Yeasts
Ye-Gi Lee,주예빈,Liang Sun,박수정,Jin Yong-Su,김수린 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.6
Numerous technological advances have been made in the depolymerization and fermentation of lignocellulosic biomass. Nevertheless, economic feasibility is still a major concern for the bioconversion of lignocellulose into value-added products. Acetate—the most abundant carbon source after the sugars in cellulosic hydrolysates— has been considered a fermentation inhibitor, but it can also be a good substrate in industrial biotechnological processes. Co-consumption of acetate and cellulosic sugars by yeasts will improve the economic feasibility of any bioconversion processes using cellulosic hydrolysates. This study investigates state-of-the-art technologies for the utilization of the hemicellulose fractions of lignocellulosic biomass, rich in acetate and xylose. Furthermore, the potentials of acetate- and xylose-rich hydrolysates will be highlighted in diversifying the product profiles for lignocellulosic bioprocesses from bioethanol to drop-in fuels and other valueadded chemicals.
Ye-Jong Park,Shin Hwang,Ki-Hun Kim,Young-Joo Lee,Chul-Soo Ahn,Deok-Bog Moon,Kwang-Min Park,Tae-Yong Ha,Gi-Won Song,Dong-Hwan Jung,Gil-Chun Park,Jae-Hun Lee,Sung-Gyu Lee 한국간담췌외과학회 2013 한국간담췌외과학회지 Vol.17 No.3
Backgrounds/Aims: Laparoscopic cholecystectomy (LC) has become a standard procedure for treatment of benign gallbladder diseases. There has been a small proportion of gallbladder cancer (GBC) which was incidentally found in the gallbladder specimen, and LC has been tried in some patients with faintly suspected GBC. This study intended to analyze the prognosis of patients with pT1b/T2 GBC who have undergone LC and the outcome of extended re-operation. Methods: After analyzing the institutional profiles of 500 GBC patients who have undergone surgical resection, we selected 64 patients who underwent LC initially from January 1996 to December 2008 and whose gallbladder pathology was confined to pT1b or pT2 lesions. Of them, 34 patients (53.1%) underwent extended reoperation. Their medical records were reviewed retrospectively. Results: In the LC only group (n=30), mean age of the 16 pT1 patients was 65.7±12.5 years and mean age of the 14 pT2 patients was 66.7±10.1 years. In the reoperation group (n=34), mean age of the 8 pT1b patients was 52.6±9.9 years and in 26 pT2 patients, mean age was 59.2±7.9 years. The reoperation group showed a younger patient age pattern than the LC only group (p=0.001). The types of reoperation were liver resection with lymph node (LN) dissection in 17, bile duct resection with LN dissection in 2, and hepatectomy and bile duct resection with LN dissection in 15. In the LC only group, the 5-year survival rate (5-YSR) was 70.3% in pT1b and 43.2% in pT2. In the reoperation group, 5-YSR was 62.5% in pT1b (n=8) and 59.5% in pT2 (n=26). A survival comparison between the two groups showed no significant survival gain in pT1 patients (p=0.69) and in pT2 patients (p=0.14). In our whole database analysis, 5-YSR of pT1bNx lesions was 70% after cholecystectomy and 78% after extended cholecystectomy. Lymph node metastasis was identified in 11% of pT1b lesions. For pT2N0 lesions, overall 5-YSR was 62% after R0 resection, showing no survival difference between primary extended surgery and LC-redo operation (p=0.45). Conclusions: The survival gain of reoperation was not evident in pT1b lesions. In contrast, some noticeable but not statistically significant survival difference was observed in pT2 lesions. Thus, reoperation for pT1b/T2 GBC following LC is indicated for individualized reasons, especially in patients with pT1b lesions. Old age was one of the important factors in deciding not to reoperate.
Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs
Seul-Gi Yang(Seul-Gi Yang),Jae-Hun Choi(Jae-Hun Choi),Young-Seo Jo(Young-Seo Jo),Ye-Won Kim(Ye-Won Kim),Dong-Mok Lee(Dong-Mok Lee),Hyo-Jin Park(Hyo-Jin Park),Deog-Bon Koo(Deog-Bon Koo) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.2
Various factors in the ovary are known to regulate oocyte maturation and hormone secretory functions; however, the effect of ovarian extract (OE) on oocyte maturation and embryonic development in pigs remains unknown. In this study, we first evaluated whether OE supplementation in the in vitro maturation (IVM) medium alters the oocyte maturation capacity by affecting glucose/amino acid metabolites, meiotic maturation, cumulus cell (CC) expansion, and antioxidants. Various OE concentrations (50, 100, 200, 500, and 5000 μg/mL) were included in the IVM medium. Only the oocytes treated with 100 μg/mL OE exhibited an improved meiotic maturation rate when compared with that of the other groups (non-treated group, 78.6 ± 3.0% vs. 100 μg/mL OE-treated group, 81.6 ± 4.3%); however, the difference was not significant. To observe the changes in glucose and amino acid metabolism in the OE-treated oocytes, we measured the amounts of diverse constituents (glucose, lactate, glutamine, and ammonia) in the IVM medium containing OE. Lactate and ammonia levels in the OE-treated group after 44 h of IVM were higher (p < 0.01) than those in the non-treated group. In addition, the expression of the CC expansion factors (Has2 and Tnfaip6) significantly increased (p < 0.05), whereas the mRNA expression levels of antioxidative enzymes (Sod1, Cat, and Gpx1) significantly diminished (p < 0.05) in the OE-treated group. Moreover, mature oocytes treated with 100 μg/mL OE demonstrated increased subsequent embryonic development rates after 144 h of IVM. Thus, the addition of OE in IVM mediums may improve oocyte maturation capacity which could enhance antioxidative enzyme activation, energy metabolism, and expression of the CC expansion factors in porcine oocytes.