전통주 주박의 항혈전 활성 평가

김미선(Mi-Sun Kim),이예슬(Ye-Seul Lee),김종식(Jong Sik Kim),신우창(Woo-Chang Shin),손호용(Ho-Yong Sohn) 한국생명과학회 2014 생명과학회지 Vol.24 No.8

전통주 주박을 이용한 고부가가치 식품소재 개발을 위해, 상업적 시설에서 생산된 3종 약주(J-B, J-S, J-Y) 및 2종 탁주(J-H, J-W) 주박의 ethanol 추출물 및 열수 추출물을 조제하고 이들의 혈액응고 저해활성, 혈소판 응집저해 활성 및 인간 적혈구 용혈활성을 평가하였다. 5종 주박의 pH는 3.90~4.29로 유사하였으나, brix는 5.0~27.0으로 다양하게 나타났으며, 수분 및 알코올 함량에서도 시료에 따라 1.8배의 차이를 나타내었다. 주박의 색차와 성분은 첨가된 부재료 및 사용누룩에 좌우되었으며, J-W 주박의 경우 수분함량이 80.3%, brix 13, 알코올 함량 1.8%를 함유하여 다른 주박에 비해 다양한 식품제조에 용이하게 이용 가능하리라 판단되었다. Ethanol 추출효율은 J-H, J-W, J-B, J-S, J-Y의 순, 열수 추출효율은 J-S, J-B, J-W, J-H, J-Y의 순으로 높았으며, 총폴리페놀 및 총플라보노이드함량은 ethanol 추출물 중에서는 J-H, 열수 추출물 중에서는 J-Y 주박에서 가장 높았다. 5종 주박의 10종 추출물은 모두 5 mg/ml 농도까지 인간 적혈구에 대한 용혈활성이 나타나지 않았으며, J-B, J-S, J-Y의 약주 주박의 ethanol 추출물에서 유의적인 혈액응고저해 활성이 나타났으며, J-W 탁주 주박의 열수 추출물에서 thrombin 저해 활성과 J-B, J-S 및 J-H 주박 열수 추출물에서 혈액 응고인자 저해활성을 확인하였다. 혈소판 응집저해 활성평가의 경우 J-W 탁주 주박의 ethanol 및 열수 추출물에서만 아스피린에 필적하는 우수한 활성이 확인되었다. 본 연구결과는 다양한 약주 및 탁주 주박이 항혈전 활성을 가지고 있으며, 주박으로부터 항혈전제 개발이 가능함을 제시하고 있다. In this study, ethanol and hot water extracts of lees from Korean traditional wine (J-B, J-S, J-Y, J-H, and J-W) were prepared, and their effects on blood coagulation, platelet aggregation, and hemolysis of human red blood cells (hRBCs) were investigated to develop functional food ingredients from lees. The pH and brix of the lees ranged from 3.90 to 4.29 and 5.0 to 27.0o, respectively, and there was a huge difference in the water and ethanol content among the lees. The nuruk and additives used affected the color and physicochemical properties of lees. The J-W takju made from only rice and traditional nuruk, which has 13o brix and 1.8% of alcohol, has potential as functional food ingredient. With regard to the extraction yields of lees, higher yields were obtained from J-H, which contains different medicinal plants, in ethanol, followed by J-W, J-B, J-S, and J-Y. Higher extraction yields of lees were obtained from J-S in hot water, followed by J-B, J-W, J-H, and J-Y, respectively. The ethanol extract of J-H and the hot water extract of J-Y had the highest contents of total polyphenol and total flavonoids among the lees extracts. The 10 lees extracts did not show hemolysis activity against hRBCs up to 5 mg/ml. In an anticoagulation activity assay, the ethanol extracts of three yakju lees (J-B, J-S, and J-Y) and the hot water extract of J-W inhibited thrombin activity, whereas the hot water extract of J-B, J-S, and J-H inhibited blood coagulation factors. In an antiplatelet aggregation activity assay, only the J-W takju lees showed significant inhibition activity. Our results suggest that lees from traditional wine had high potential as a novel antithrombosis agent.

Identification of determinants of the utilisation of acupuncture treatment using Andersen's behavioural model

Lee, Ye-Seul,Lee, In-Seon,Kim, Song-Yi,Lee, Hyangsook,Park, Hi-Joon,Lee, Hyejung,Kim, Sung-Hoon,Chae, Younbyoung THE BRITISH MEDICAL ACUPUNCTURE SOCIETY 2015 ACUPUNCTURE IN MEDICINE Vol.33 No.2

<P><B>Objectives</B></P><P>The utilisation of healthcare services is the result of a complex decision-making process with different determinants. This study aims to identify the determinants of the utilisation of acupuncture treatment using Andersen's behavioural model and quality of life.</P><P><B>Methods</B></P><P>In this study we reviewed Korean Health Panel data, a nationally representative set of data, and analysed the utilisation of outpatient acupuncture services during 2011. A two-part model, which included predisposing, enabling and need factors of Andersen's behavioural model in model 1 and the additional factor of health-related quality of life (HRQOL, EQ-5D Korean index) in model 2, was analysed by logistic analysis to identify determinants of acupuncture service usage among subjects who visited traditional Korean medical clinics.</P><P><B>Results</B></P><P>The higher frequency of acupuncture service usage among patients who visited traditional Korean medical clinics was associated with gender, age, number of chronic diseases, physical disabilities and HRQOL of the subjects.</P><P><B>Conclusions</B></P><P>This study shows that the important determinants of the frequency of acupuncture treatment utilisation are gender, age, number of chronic diseases and the presence of physical disabilities. Our findings contribute to understanding the characteristics of patients who use acupuncture treatment and may be used as a basic resource for related policy making by government officials and medical professionals.</P>

Effect of Optimum Nutrient Management on Productivity and Nitrogen Balance in Rice Cultivation: A Review

Seul-Bi Lee,Yang-Min Kim,Ye-Jin Lee,Yo-Sung Song,Cheol-Hyun Ryu,Deog-Bae Lee,Chan-Wook Lee,Chang-Hoon Lee,Jwakyung Sung 한국토양비료학회 2019 한국토양비료학회지 Vol.52 No.4

Given continuous needs for a public duty of agriculture and rural area, an effort to improve nutrient management in agricultural sector is strongly required. As a measure to solve these problems, Korean government (MAFRA) introduced the promotion policy called as “Agro-Environment Conservation Program (AECP)” in 2018 and has expanded 5 projet sites in 2019 from 3 in 2018. The program has a variety of agricultural activity to conserve soil, water, air, ecology and landscape. Of those, the nutrient input activity which is one of sub-activities is closely involved in not only crop productivity but also water quality and air pollution. Therefore, in order to lead a soft landing of AECP, it is essential to evaluate the conservation effects in a scientific point of view. In this respect, we focused on evaluating the effect of recommended nutrient input activities, standard fertilization and soil test fertilization, on rice productivity and nitrogen balance. The effect of nutrient management which is explained by rice yield and nitrogen balance was estimated with the combination of soil characteristics and rice varieties. Rice yield by recommended fertilization was 654 kg per 10a, 6% lower than control, but there was no statistical significance. By contrast, nitrogen balance was 4.7 kg lower, which means great improvement, in the recommended than the control. Conclusively, the recommended fertilization like soil test fertilization is expected to be useful as a measure to reduce nutrient balance, to ensure crop productivity, and finally to perform a public role of agriculture.

고구마 소주 주박의 항균 및 항혈전 활성

김미선 ( Mi Sun Kim ),이예슬 ( Ye Seul Lee ),김종식 ( Jong Sik Kim ),신우창 ( Woo Chang Shin ),손호용 ( Ho Yong Sohn ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.3

Sweet potato soju (SPS), a form of traditional distilled alcoholic liquor in Korea, is manufactured by the distillation of fermented broth under normal pressure, thus providing it for a uniquely smooth taste infused with the flavor of sweet potato. After distillation, the lees of SPS is produced as by-product and discarded. In this study, the ethanol and hot water extracts of lees of SPS, and their subsequent organic solvent fractions using hexane, ethylacetate (EA), butanol, and water residue were prepared in an effort at the efficient re-use of the lees of SPS. The ethanol extraction yield was 1.36-fold higher than that of the hot water extraction, and the EA fraction revealed the highest total polyphenol content among the solvent fractions. The various extracts and solvent fractions did not demonstrate hemolytic activity at up to 0.5 mg/ml concentrations against human red blood cells. In the bio-activity assay, only the EA fraction displayed a broad spectrum of anti-microbial activity against different pathogenic and food spoilage bacteria, and demonstrated significant anti-coagulation activity by inhibitions of thrombin, prothrombin and blood coagulation factors. Furthermore, only the EA fraction from the hot water extract of the lees of SPS showed anti-platelet aggregation activity, which is comparable to aspirin (a commercially available drug). Our results suggest that the EA fraction of the hot water extract prepared from the lees of SPS has a high potential as a novel resource for anti-microbial and anti-thrombosis agents.

Analysis of Social Network Service User Responses and Success Factors for TV Dance Programs

Ye-Seul Lee 상명대학교 글로벌문화예술교육연구소 2020 Research in Dance and Physical Education Vol.4 No.2

FC 2-7 : Elevated KAI1 protein expression identified in malignant melanoma

( Ye Seul Kim ),( Ji Hoon Sim ),( Sang Jin Oh ),( Je Min An ),( Sang Hoon Lee ),( Sung Yul Lee ),( Moon Kyun Cho ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: KAI1/CD82 reportedly slows the metastatic process in a variety of tumors. However, its function is not fully clear. This study investigates the expression of KAI1 in correlation with malignant skin cancers. Objectives: To investigate the expression and clinical significance of KAI1 protein in malignant skin cancers. Methods: KAI1/CD82 expression was evaluated by western blotting in 24 specimens including 6 malignant melanoma (MM), 6 squamous cell carcinoma (SCC), 6 BCC and 6 normal skin specimens. Immunohistochemical staining was performed to measure KAI1/CD82 expression in 6 MM, 6 SCC and 6 BCC. Results: We observed over-expression of KAI1 in the malignant melanoma. The expressions of KAI1 in malignant melanoma were higher than those in SCC and BCC. The KAI1 expressions in malignant melanoma were higher in protein levels compared to SCC and BCC. Western blotting results agreed with immunohistochemistry. Conclusion: KAI1 is highly related to malignant melanoma and may be a useful indicator of malignant melanoma.

Yield and Free Sugar Contents of Burdock (Arctium lappa L.) depending on Nitrogen Levels

Ye-Jin Lee,Jwa-Kyung Sung,Seul-Bi Lee,Jung-Eun Lim,Yo-Sung Song,Deog-Bae Lee 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.6

Excessive nitrogen fertilization influences crop yields and quality as well as environmental pollution. In this study, yields, nitrogen use efficiency and free sugar contents of burdock (Arctium lappa L.) were evaluated at different levels of nitrogen fertilization. Nitrogen fertilizer was applied at 5 levels (0, 50, 100, 150, 200%) based on the conventional fertilization (N = 230 kg ha<SUP>-1</SUP>), and phosphate and potassium fertilizer were treated by conventional P and K fertilization (P₂O5 - K₂O = 140 - 210 kg ha<SUP>-1</SUP>) in all plots. The root yields of burdock were the highest in N 100~150% treatment plots. Nitrogen use efficiency and nitrogen recovery decreased from over N 150% treatment. Nitrogen uptake of root was greater than that of shoot in N 50~200% treatments. Fructose contents in root were inversely proportional to the level of nitrogen fertilization. As considering nitrogen recovery and root quality, economical burdock yield was obtained in N 230 kg ha<SUP>-1</SUP>.

Plant Analysis Methods for Evaluating Mineral Nutrient

Ye-Jin Lee,Jwa-Kyung Sung,Seul-Bi Lee,Jung-Eun Lim,Yo-Sung Song,Deog-Bae Lee,Suk-Young Hong 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.2

Analysis of mineral nutrients in plant is required for evaluating diagnosis of plant nutritional status. Pretreatment procedure for the analysis of plant can be varied depending on elements to be analyzed. Wet-digestion is suitable for total nitrogen, phosphate and cations, however, digestion solution including nitric acid is not suitable for nitrogen analysis. Incineration procedure is required to analyze chloride, silicate and total sulfur. After digestion, total nitrogen is analyzed by Kjeldahl method, and phosphate is detected at 470nm by colorimetric analysis with ammonium meta vanadate. Cations and micro elements are determined by titration or colorimetry, also, these elements can be measured by Atomic absorption spectrometer (AAS) or Inductively coupled plasma spectrometer (ICP).

Newly Designed Copolymers for Organic Photovoltaics Investigating the Structure-Property Relations

Ye Seul LEE,Seyeong SONG,Soon-Ki KWON,Jin Young KIM,Yun-Hi KIM 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

How do readers choose to undergo treatments based on medical articles?

Lee, Ye-Seul,Kim, Jeongjoo,Joo, Seongsu,Go, Byeongho,Lee, Hyangsook,Chae, Younbyoung Williams & Wilkins Co 2016 Medicine Vol.95 No.49

<P><B>Abstract</B></P><P>The purpose of this study is to study the reader's cognitive process in reading medical articles and its influence on the decision-making process. Twenty-four participants completed a survey pertaining to 36 medical articles on new treatments with similar text structures and similar titles. Participants rated each article on its level of interest, informativeness, and reliability, and were asked whether they would choose the treatments in the news article. A cognitive decision-making model can be applied to health contexts, in which the reader's subjective ratings on interest, informativeness, and reliability were positively associated with choosing new treatments. The decision-making process path from the perception of informativeness was mediated by the reliability of the news article. Interest was positively linked to informativeness, although it was not directly associated with reliability. This study shows that readers’ subjective ratings on health information can indicate their decision-making. Artifacts in the information that might incite emotions or interest, such as framing, can affect the reader's decision-making process.</P>