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고려 엉겅퀴(Cirsium setidens Nakai) 잎 조직을 이용한 callus 배양 및 항산화 활성 검증
박정훈,심예지,박기임,이인순,문혜연 대구대학교 특수교육재활과학연구소 2010 産業技術硏究 Vol.21 No.1
We described the effects of antioxidant activity on the extracts of tissue and callus formation by Cirsium setidens Nakai. The callus culture were induced from leaf tissue of Cirsium setidens in both MS and B5 medium with IAA or/and kinetin (0∼5.0 ㎎/ℓ). As medium for callus culture induced from Cirsium setidens, B5 medium showed better condition than MS media. MS medium brought to decrease callus differentiation after formation, on the other hand B5 medium induced to accelerate callus differentiation. Among phytohormones is required 0.5 ㎎/ℓ of IAA and between 2.0 ㎎/ℓ and 1.0 ㎎/ℓ of Kinetin for formation of callus. In order to extract functional material from natural leaf and callus tissue of Cirsium setidens used distilled water and 75% ethanol as solvent and those extract used to determine antioxidant activity as alpha-diphenyl-2-picryl-hydrozyl (DPPH) radicals scavenging effect and TBA(thiobarbituric acid) activity. In result, the effect of DPPH and TBA had high activity more natural leaf extract than callus tissue and 75% ethanol was solvent better than distilled water. The 75% ethanol extract of natural leaf showed an excellent antioxidant activity because that particularly had highest concentration of vitamin C and total phenol among those extracts
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Acetate-rich Cellulosic Hydrolysates and Their Bioconversion Using Yeasts
Ye-Gi Lee,주예빈,Liang Sun,박수정,Jin Yong-Su,김수린 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.6
Numerous technological advances have been made in the depolymerization and fermentation of lignocellulosic biomass. Nevertheless, economic feasibility is still a major concern for the bioconversion of lignocellulose into value-added products. Acetate—the most abundant carbon source after the sugars in cellulosic hydrolysates— has been considered a fermentation inhibitor, but it can also be a good substrate in industrial biotechnological processes. Co-consumption of acetate and cellulosic sugars by yeasts will improve the economic feasibility of any bioconversion processes using cellulosic hydrolysates. This study investigates state-of-the-art technologies for the utilization of the hemicellulose fractions of lignocellulosic biomass, rich in acetate and xylose. Furthermore, the potentials of acetate- and xylose-rich hydrolysates will be highlighted in diversifying the product profiles for lignocellulosic bioprocesses from bioethanol to drop-in fuels and other valueadded chemicals.
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Lee, Ye-Gi,Jin, Yong-Su,Cha, Young-Lok,Seo, Jin-Ho Elsevier 2017 Bioresource technology Vol.228 No.-
<P><B>Abstract</B></P> <P>Even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. This study demonstrates that an industrial polyploidy <I>Saccharomyces cerevisiae</I> JHS200 can be engineered through Cas9 (CRISPR associated protein 9)-based genome editing. Specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into the auxotrophic mutants. As expected, the engineered strain (JX123) enhanced ethanol production from cellulosic hydrolysates as compared to other engineered haploid strains. However, the JX123 strain produced substantial amounts of xylitol as a by-product during xylose fermentation. Hypothesizing that the xylitol accumulation might be caused by intracellular redox imbalance from cofactor difference, the NADH oxidase from <I>Lactococcus lactis</I> was introduced into the JX123 strain. The resulting strain (JX123_noxE) not only produced more ethanol, but also produced xylitol less than the JX123 strain. These results suggest that industrial polyploidy yeast can be modified for producing biofuels and chemicals.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Industrial <I>Saccharomyces cerevisiae</I> was able to edit genome by CRISPR-Cas9. </LI> <LI> A xylose assimilating pathway was introduced into an industrial yeast strain. </LI> <LI> Expression of NADH oxidase improved the bioethanol productivity. </LI> <LI> The concentration of ethanol produced by silver grass hydrolysates is 55.51g/L. </LI> </UL> </P>
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Ye-Jong Park,Shin Hwang,Ki-Hun Kim,Young-Joo Lee,Chul-Soo Ahn,Deok-Bog Moon,Kwang-Min Park,Tae-Yong Ha,Gi-Won Song,Dong-Hwan Jung,Gil-Chun Park,Jae-Hun Lee,Sung-Gyu Lee 한국간담췌외과학회 2013 한국간담췌외과학회지 Vol.17 No.3
Backgrounds/Aims: Laparoscopic cholecystectomy (LC) has become a standard procedure for treatment of benign gallbladder diseases. There has been a small proportion of gallbladder cancer (GBC) which was incidentally found in the gallbladder specimen, and LC has been tried in some patients with faintly suspected GBC. This study intended to analyze the prognosis of patients with pT1b/T2 GBC who have undergone LC and the outcome of extended re-operation. Methods: After analyzing the institutional profiles of 500 GBC patients who have undergone surgical resection, we selected 64 patients who underwent LC initially from January 1996 to December 2008 and whose gallbladder pathology was confined to pT1b or pT2 lesions. Of them, 34 patients (53.1%) underwent extended reoperation. Their medical records were reviewed retrospectively. Results: In the LC only group (n=30), mean age of the 16 pT1 patients was 65.7±12.5 years and mean age of the 14 pT2 patients was 66.7±10.1 years. In the reoperation group (n=34), mean age of the 8 pT1b patients was 52.6±9.9 years and in 26 pT2 patients, mean age was 59.2±7.9 years. The reoperation group showed a younger patient age pattern than the LC only group (p=0.001). The types of reoperation were liver resection with lymph node (LN) dissection in 17, bile duct resection with LN dissection in 2, and hepatectomy and bile duct resection with LN dissection in 15. In the LC only group, the 5-year survival rate (5-YSR) was 70.3% in pT1b and 43.2% in pT2. In the reoperation group, 5-YSR was 62.5% in pT1b (n=8) and 59.5% in pT2 (n=26). A survival comparison between the two groups showed no significant survival gain in pT1 patients (p=0.69) and in pT2 patients (p=0.14). In our whole database analysis, 5-YSR of pT1bNx lesions was 70% after cholecystectomy and 78% after extended cholecystectomy. Lymph node metastasis was identified in 11% of pT1b lesions. For pT2N0 lesions, overall 5-YSR was 62% after R0 resection, showing no survival difference between primary extended surgery and LC-redo operation (p=0.45). Conclusions: The survival gain of reoperation was not evident in pT1b lesions. In contrast, some noticeable but not statistically significant survival difference was observed in pT2 lesions. Thus, reoperation for pT1b/T2 GBC following LC is indicated for individualized reasons, especially in patients with pT1b lesions. Old age was one of the important factors in deciding not to reoperate.
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Lee, Chung-Gi,Song, Ye Jin,Lee, Ki Bong,Mun, Sungyong Elsevier 2019 Journal of industrial and engineering chemistry Vol.80 No.-
<P><B>Abstract</B></P> <P>To maximize the industrial value of biologically produced 2,3-butanediol (BD), it is essential to separate BD into its stereoisomeric fractions, <I>meso</I>-BD and <I>racemic</I>-BD. To address such issue, a highly efficient BD-isomer separation process based on a simulated-moving-bed (SMB) mode was developed in this study. As a first step for this task, the competitive adsorption behaviors of <I>meso</I>-BD and <I>racemic</I>-BD on a properly selected adsorbent were investigated, and their relevant isotherm parameters were determined using inverse method. The determined isotherm parameters were then applied to the design of the BD-isomer separation process based on an SMB mode, which was carried out in such a way that both BD isomers could be separated with high purities and little losses in a continuous countercurrent mode. The performance of the designed SMB process for the targeted separation was experimentally investigated. It was confirmed from the experimental results that the BD-isomer separation SMB process, which was developed in this study for the first time, could be effective in the continuous-mode separation of <I>meso</I>-BD and <I>racemic</I>-BD with the purities higher than 99.9% and 97% respectively while maintaining their losses below 1%. The results of this study are expected to pave the way for establishing a highly economical process for a large-scale production of <I>meso</I>-BD and <I>racemic</I>-BD with high purities.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The SMB for continuous-mode separation of 2,3-butanediol (BD) isomers was developed. </LI> <LI> Highly effective adsorbent for separation of <I>meso</I>-BD and <I>racemic</I>-BD was found. </LI> <LI> Competitive Langmuir isotherm parameters of BD isomers were determined and validated. </LI> <LI> The resultant isotherm parameters were used to design the BD-isomer separation SMB. </LI> <LI> The designed SMB led to high purities of <I>meso</I>-BD and <I>racemic</I>-BD with little losses. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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이예지(Ye Gi Lee),하중한(Jung Jan Ha),임희준(Hee Jun Lim),장현국(Hyunkook Jang),이희연(Hee-Youn Lee) 한국HCI학회 2019 한국HCI학회 학술대회 Vol.2019 No.2
본 연구는 스마트폰 앱에서 사용자가 실시간으로 치아를 닦는 부위를 확인할 수 있도록 제공한다. 이를 위해 RNN(Recurrent Neural Network)을 이용하여 데이터를 학습시켜 모델을 저장하고 앱에 담아 실시간 치아부위를 측정할 수 있게 하였다. 실험방법은 블루투스를 통해 하드웨어가 측정한 센서데이터 값을 데이터 수집 앱에 저장하고, 이 데이터를 활용해 RNN 을 학습시킨 후 신경망 모델을 저장한다. 그 후 스마트 폰에서 학습된 모델을 Tensorflow lite 를 통해 불러와 실시간 치아 부위를 표시하였다. 위와 같은 실험을 한 결과 실시간으로 사용자의 칫솔질을 파악할 수 있었으며, 이러한 데이터 들로 사용자의 칫솔질 패턴을 파악해 사용자에게 ‘현재 나의 칫솔질이 어떠한가?’에 대해 알려줌으로써 사용자가 올바르게 양치 할 수 있도록 도와줄 수 있을 것이라 생각한다.
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정신치료 수련의 질과 역량에 관한 정신건강의학과 전공의 인식도 조사
김예지(Ye Ji Kim),견영기(Yeong Gi Kyeon),이승재(Seung Jae Lee),전명욱(Myong-Wuk Chon),이상원(Sang Won Lee),정성원(Sung Won Jung),이강욱(Kang Uk Lee),대한신경정신의학회 수련위원회(The Committee of Residency Training of the Korean Neurop 대한신경정신의학회 2021 신경정신의학 Vol.60 No.3
Objectives This study surveyed the quality of psychotherapy training and psychotherapy competencies during residency in Korea. Methods A questionnaire developed by The Committee of Residency Training of the Korean Neuropsychiatric Association was completed by fourth-year residents of Korean psychiatry (n=120). The questionnaire examined their demographic characteristics, their experience of clinical psychotherapy training, their degree of satisfaction in the quality of psychotherapy training, and self-rated competence regarding the six types of psychotherapies, including supportive psychotherapy, cognitive behavioral therapy, interpersonal psychotherapy, psychoanalytic psychotherapy, group psychotherapy, and family psychotherapy. Results The degree of satisfaction of the residents with the psychotherapy training was diverse among the types of psychotherapy and contents of training. Across all types of psychotherapies, the satisfaction levels of the quantity and quality of supervision education were low, except for psychoanalytic psychotherapy. Overall, more than 70% of residents were not satisfied with the theoretical education, clinical practice, and supervision education in three types of psychotherapies, including interpersonal psychotherapy, group psychotherapy, and family psychotherapy. A positive correlation was found between the degree of satisfaction with the quality of training experience and self-rated competence of psychotherapy. Specifically, the self-rated competence of psychotherapy was more highly correlated with the experience of clinical cases and supervision than theoretical education Conclusion This study showed the quantitative and qualitative status of psychotherapy training in Korean psychiatry residency programs, and the satisfaction level of psychotherapy education was low except for psychoanalytic psychotherapy. There is a strong need for the improvement of psychotherapy training programs in Korea.
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