Phylogenetic analysis of necleotide sequences from the ITS region of fourteen species of Tilletia

(Gui Ming Zhang),(Yi Jian Yao),(Hui Ying Cheng) 한국균학회 2001 Proceedings of the Fifth Korea-China Joint Symposi Vol.- No.-

Flower cultivation regimes aff ect apocarotenoid accumulation and gene expression during the development of saff ron stigma

Gui-Fen Zhou,Li-Qin Li,Jiemiao Lu,Jing Li,Chong Yao,Peng Sun,Kaohua Liu,Yan Dong,Luping Qin,Xiao-Dong Qian 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.3

The quality of commercial saff ron largely depends on apocarotenoid levels. Whether they are aff ected by diff erent fl owercultivation regimes remains unknown. In present study, apocarotenoid levels and the expression profi les of 12 genes relatedto apocarotenoid biosynthesis between fi eld- and indoor-cultivated Crocus sativus L. were analyzed during stigma development. Under both cultivation regimes, apocarotenoid accumulation and gene expression showed similar profi les, fi rstsharply increasing and then gradually decreasing with stigma development, which suggested the coordinated function ofgene expression and apocarotenoid accumulation. However, the rate of increase/decrease varied widely between two blossomregimes at diff erent developmental stages. Based on correlation analysis, βCH1 and ZDS seemed to play a more crucial rolein apocarotenoid accumulation under both cultivation regimes. At anthesis, the total apocarotenoid level in saff ron cultivatedin the fi eld was higher than that cultivated indoors. This may be due to lower gene expression levels in the apocarotenoidpathway, especially βCH1 , ZDS , and CCD2 , and higher CCD4a and NCED expression in the competitive pathway in saff roncultivated indoors than that in the fi eld, which indicated higher conversion in apocarotenoid biosynthesis fl ux from crocinsand picrocrocin synthesis to β-ionone and the ABA biosynthetic branch in saff ron cultivated indoors. These results providevaluable information for modifying planting mode to enhance saff ron quality.

Effects of lycopene on number and function of human peripheral blood endothelial progenitor cells cultivated with high glucose

Yao-Chi Zeng,Gui-Ping Mu,Shu-Fen Huang,Xue-Hui Zeng,Hong Cheng,Zhong-Xin Li 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.4

BACKGROUND/OBJECTIVES: The objectives of this study were to investigate the effects of lycopene on the migration, adhesion, tube formation capacity, and p38 mitogen-activated protein kinase (p38 MAPK) activity of endothelial progenitor cells (EPCs) cultivated with high glucose (HG) and as well as explore the mechanism behind the protective effects of lycopene on peripheral blood EPCs. MATERIALS/METHODS: Mononuclear cells were isolated from human peripheral blood by Ficoll density gradient centrifugation. EPCs were identified after induction of cellular differentiation. Third generation EPCs were incubated with HG (33 mmol/L) or 10, 30, and 50 μg/mL of lycopene plus HG. MTT assay and flow cytometry were performed to assess proliferation and apoptosis of EPCs. EPC migration was assessed by MTT assay with a modified boyden chamber. Adhesion assay was performed by replating EPCs on fibronectin-coated dishes, after which adherent cells were counted. In vitro vasculogenesis activity was assayed by Madrigal network formation assay. Western blotting was performed to analyze protein expression of both phosphorylated and non-phosphorylated p38 MAPK. RESULTS: The proliferation, migration, adhesion, and in vitro vasculogenesis capacity of EPCs treated with 10, 30, and 50 μg/mL of lycopene plus HG were all significantly higher comapred to the HG group (P < 0.05). Rates of apoptosis were also significantly lower than that of the HG group. Moreover, lycopene blocked phosphorylation of p38 MAPK in EPCs (P < 0.05). To confirm the causal relationship between MAPK inhibition and the protective effects of lycopene against HG-induced cellular injury, we treated cells with SB203580, a phosphorylation inhibitor. The inhibitor significantly inhibited HG-induced EPC injury. CONCLUSIONS: Lycopene promotes proliferation, migration, adhesion, and in vitro vasculogenesis capacity as well as reduces apoptosis of EPCs. Further, the underlying molecular mechanism of the protective effects of lycopene against HG-induced EPC injury may involve the p38 MAPK signal transduction pathway. Specifically, lycopene was shown to inhibit HG-induced EPC injury by inhibiting p38 MAPKs.

A Fast Rough Mode Decision Algorithm for HEVC

Yao, Wei-Xin,Yang, Dan,Lu, Gui-Fu,Wang, Jun Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.3

HEVC is the high efficiency video coding standard, which provides better coding efficiency contrasted with the other video coding standard. But at the same time the computational complexity increases drastically. Thirty-five kinds of intra-prediction modes are defined in HEVC, while 9 kinds of intra prediction modes are defined in H.264/AVC. This paper proposes a fast rough mode decision (RMD) algorithm which adopts the smoothness of the up-reference pixels and the left-reference pixels to decrease the computational complexity. The three step search method is implemented in RMD process. The experimental results compared with HM13.0 indicate that the proposed algorithm can save 39.7% of the encoding time, while Bjontegaard delta bitrate (BDBR) is increased slightly by 1.35% and Bjontegaard delta peak signal-to-noise ratio (BDPSNR) loss is negligible.

Toughening polystyrene by core–shell grafting copolymer polybutadiene-graft-polystyrene with potassium persulfate as initiator

Gui Di Cai,Guang Hui Gao,Hong Yu Yang,Li Dan Zhu,Hua Liu,Guang Feng Wu,Ming Yao Zhang,Chao Zhou,Hui Xuan Zhang 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.3

Core–shell polybutadiene-graft-polystyrene rubber particles with different ratios of polybutadiene core to polystyrene shell were synthesized by an emulsion polymerization using K2S2O8 as an initiator. Then the core–shell rubber particles were blended with PS to prepare PS/PB-g-PS. The rubber particles with a size of 0.3–0.5 mm could toughen polystyrene significantly. The mechanical properties, morphologies and deformation mechanisms of samples were extensively investigated. The experimental results showed that the dispersion of rubber particles in a ‘‘cluster’’ state leads to better impact resistances. Crazing occurred from rubber particles and extended in a bridge-like manner to neighboring rubber particles parallel to the equatorial direction.

Virtual boundary element-equivalent collocation method for the plane magnetoelectroelastic solids

Yao, Wei-An,Li, Xiao-Chuan,Yu, Gui-Rong Techno-Press 2006 Structural Engineering and Mechanics, An Int'l Jou Vol.22 No.1

This paper presents a virtual boundary element-equivalent collocation method (VBEM) for the plane magnetoelectroelastic solids, which is based on the fundamental solutions of the plane magnetoelectroelastic solids and the basic idea of the virtual boundary element method for elasticity. Besides all the advantages of the conventional boundary element method (BEM) over domain discretization methods, this method avoids the computation of singular integral on the boundary by introducing the virtual boundary. In the end, several numerical examples are performed to demonstrate the performance of this method, and the results show that they agree well with the exact solutions. So the method is one of the efficient numerical methods used to analyze megnatoelectroelastic solids.

Basic Properties and Engineering Application of Bentonite-Cement-Water Glass Grouting

Yao Zhou,Gui He Wang,Yu Fei Yuan 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.9

The cement manufacturing process causes environmental problems such as carbon dioxide emissions. Bentonite-cement-water glass grouting (BCG) is a grout with low environmental impact, designed to reduce the use of cement. The significance of this study is to explore the fundamental properties of BCGs with the bentonite contents of up to 50%, and the antiwash-out and permeability characteristics of BCG in comparison with cement-water glass grout (CG). The effects of water-to-solid ratios (W/S), bentonite content, water glass content and the volume ratio of bentonite-cement slurry to water glass slurry (Va: Vb) on the fundamental properties of BCGs were tested in the laboratory. The results show that the BCGs have excellent characteristics of high viscosity, low bleeding, short and controllable setting time, and high early strength. The BCGs have been applied to the pre-grouting of the water-rich sandy pebble stratum in the Wangfujing North Station section of Beijing Metro Line 8 and achieved better water sealing and reinforcement effects than CG.

Comparison of the effect of lithium bentonite and sodium bentonite on the engineering properties of bentonite-cement-sodium silicate grout

Zhou, Yao,Wang, Gui H.,Chang, Yong H. Techno-Press 2020 Advances in concrete construction Vol.9 No.3

This paper focuses on the engineering properties of Bentonite-Cement-Sodium silicate (BCS) grout, which was prepared by partially replacing the ordinary Portland cement in Cement-Sodium silicate grout with lithium bentonite (Li-bent) and sodium bentonite (Na-bent), respectively. The effect of different Water-to-Solid ratio (W/S) and various replacement percentages of bentonite on the apparent viscosity, bleeding, setting time, and early compressive strength of BCS grout were investigated. The XRD method was used to detect its hydration products. The results showed that both bentonites played a positive role in the stability of BCS grout, increased its apparent viscosity. Na-bent prolonged the setting time of BCS, while 5% of Li-bent shortened the setting time of BCS. The XRD analysis indicated that the hydration products between the mixture containing Na-bent and Li-bent did not differ much. Using bentonite as supplementary cementitious material (SCM) to replace partial cement is a promising way to cut down on carbon dioxide emissions and to produce low-cost, eco-friendly, non-toxic, and water-resistant grout. In addition, Li-bent was superior to Na-bent in improving the strength and the thickening of BCS grouts.

Research Articles : The Influence of Challenge on Cathepsin B and D Expression Patterns in the Silkworm Bombyx mori L

( Feng Yao Wu ),( Feng Ming Zou ),( Jun Qiang Jia ),( Sheng Peng Wang ),( Guo Zheng Zhang ),( Xi Jie Guo ),( Zhong Zheng Gui ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.23 No.1

Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that Bombyx mori cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RTPCR was performed to detect cathepsin gene expression at the transcription level when challenged by B. mori nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the 5th instar, and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the 5th instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.

A comprehensive analysis and comparison between vacuum and electric oven drying methods on Chinese saffron (Crocus sativus L.)

Chong Yao,Xiao-Dong Qian,Gui-Fen Zhou,Shu-Wei Zhang,Li-Qin Li,Qiaosheng Guo 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2

The red stigmas of saffron are one of the mostexpensive spices in the world and serve as a traditionalChinese medicine. More saffron has been cultivated inChina, and different drying technologies have been studied. However, a comprehensive and comparative analysis ofdifferent drying approaches has not been well studied. Inthis study, we compared electric oven and vacuum ovendrying approaches on saffron. We found saffron was driedquicker under high vacuum drying mode with high temperatureand the quicker drying rate provided, the moreopen microstructural interstices on the saffron surface. Both methods were best fit to Midilli and Kucuk model. Besides, the coloring, aroma and bitterness strength afterdrying showed the similar results. In sum, our data suggestedthe optimal drying temperature was 100 C for20 min for two evaluated methods, however consideringthe machine cost, the electric oven drying would be the firstchoice.