In vitro propagation of Coleus forskohlii, an important medicinal plant

Yang,Deok-hun,Jeon,Manju-Meluttu-George,Jong-Seong 한국자원식물학회 2003 Plant Resources Vol.6 No.2

For mass multiplication of an important medicinal plant Coleus forskohlii, a procedure for the high frequency regeneration of Coleus forskohlii has been developed using leaf explants via callus culture. Callus formation occurred in MS medium supplemented with 1-2 mg/L each of NAA and BAP. A large number of shoots were formed on MS + 1 mg/L BAP from 50-60 days old greenish calli. Rooting of healthy shoots occurred on 0.1-0.4 mg/L NAA. The protocol described could be useful in future for genetic manipulation of this plant species.

신장내 국소혈류량 조절에 대한 신교감신경의 영향

양훈모,민영기,김동진,송호연,송윤섭 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Control of blood pressure is affected by regulation of intrarenal blood flow. Renal sympathetic nerve simulation inhibits pressure-depressor mechanism of kidney. This antidepressor mechanism was known as happening to due to a decrease the blood supply of renal medulla. It is not known whether renal sympathetic nerve innervation of renal medulla is and it affects regulation of medullary blood flow. We tried to measure total renal blood flow(TRBF), cortical blood flow(CBF), papillary blood flow(PBF) by ultrasonic and laser Doppler techniques in Wistar rats during 3 kinds of renal sympathetic nerves stimulation. The simulation orders are tried randomly and each stimulation period is 15 minute. TRBF and CBF decreased at 1 Hz by 2 and 2%, 3Hz by 18 and 15%, 5Hz by 49 and 44%, respectively as similar to each other. But PBF increased by 1% at 1 Hz and 4% at 3 Hz, while it decreased by 4% at 5 Hz. Therefore, it seems superficial renocortical and total renal blood flows are closely regulated by renal sympathetic nerves with increasing vasoconstriction at 5 Hz, onthe other hand while medullary blood flow seems to be under strong local control, tending to offset neurogenic flows restrictions.

韓國人血液群 A,B,및 AB 赤血球의 被疑集性에 關한 硏究

梁學道,李楨浩,尹秉文,芮薰海,全東雨,李文玉,金晉郁 醫藥係社 1955 綜合 醫學 Vol.12 No.8

심미수복 재료의 마모와 화학적 분해

양규호,최남기,김훈주,김선미 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

수복재료의 요건으로서 치아의 저작기능과 심미성을 회복할 수 있는 물리적, 화학적 성질뿐만 아니라 생물학적 적합성과 구강내 환경변화에 대한 내구성을 들 수 있다. 불소 방출의 장점을 갖는 컴포머나 시술시간을 줄이는데 유리한 재료인 유동성 복합레진을 유구치부에 사용하려고 할 때 마모저항성과 구강내 환경에서의 분해저항성은 중요한 물성 중 하나이다. 실험에 사용된 복합레진은 최근에 시판되고 있는 Charmfil(Denkist, Korea)과 유동성인 Charmfil flow(Denkist, Korea)이고, 컴포머는 Compoglass F(Ivoclar Vivadent, Liechtenstein)와 유동성인 PrimaFlow(DMG Hamburg, Germany)이었다. 각 제품의 분해저항성과 마모도를 평가하고자 마모시험 후 마모된 면의 깊이를 측정하였고 알칼리성 용액에 보관 시 각 제품의 분해저항성을 무게손실, 표면하 분해층 깊이, 용출된 Si 농도를 기준으로 평가하였고 주사전자현미경과 공촛점 레이저 현미경으로 분해층을 관찰하여 다음과 같은 결과를 얻었다. 1. 무게손실량은 각 제품간 차이를 보이지 않았다(p>0.05). 2. 분해층 깊이는 Compoglass F가 가장 깊었고, PrimaFlow, Charmfil, Charmfil flow 순이었고 Compoglass P와 다른 제품 간에는 유의한 차이를 보였다(p<0.05). 3. Si 용출량은 Charmfil flow가 가장 많았고, Compoglass F가 가장 작았으며 두 제품 간에는 유의한 차이를 보였다(p<0.05). 4. 주사전자현미경 관찰시 표면 양상 및 분해층 깊이를 관찰할 수 있었고 공촛점 레이저 현미경 관찰시 NaOH 용액에 보관한 후 수복재의 기질과 충전제 사이의 결합의 파괴 양상인 분해층 깊이를 관찰할 수 있었다. 5. 마모는 Compoglass F에서 가장 많이 일어났으며, PrimaFlow, Charmfil, Charmfil flow 순이었고 각 제품 간에는 유의한 차이를 보였다.(p<0.05). 6. 각 제품의 Si 용출량과 분해층 깊이 사이(r=0.602, p<0.05), 마모 최대 깊이와 비커스 경도 사이(r=0.501, p<0.05)에는 유의한 상관관계를 보였으나 Si 용출량과 마모 최대 깊이 등 다른 항목간에는 유의한 상관관계를 보이지 않았다.(r=-0.052, p>0.05). 본 연구에서 Compoglass F는 불소 함량은 가장 높았으나 화학적 분해층과 마모깊이가 가장 깊은 것으로 나타났으며 flowable type의 복합레진과 컴포머는 표면 경도와 마모도에서 양호한 결과를 보였다. 이상의 결과 복합레진과 컴포머의 평가요소로서 마모도와 함께 가수분해도 고려되어야 할 것으로 사료된다. The aim of this study was to evaluate the resistance to degradation and to compare the wear resistance characteristics of four esthetic restorative materials in an alkaline solution. The brands studied were Charmfil, Charmfil flow(composite resin), Compoglass F and PrimaFlow(compomer) . The results were as follows: 1. The mass loss were not significantly different among the materials(p〉0.05). 2. The sequence of the degree of degradation layer depth was in descending order by Compoglass F, PrimaFlow. Charmfil, and Charmfil flow. There were significant differences between Compoglass F and the others(p<0.05). 3. The sequence of the Si loss was in descending order by Charmfil flow, Charmfil, PrimaFlow, and Compoglass F. There were significant differences among these materials(p<0.05). 4. When observed with SEM, destruction of bonding between matrix and filler was observed and when observed with CLSM, the depth of degradation layer of specimen surface was observed. 5. The sequence of maximum wear depth was in descending order by Comfoglass, PrimaFlow, Charmfil, and Charmfil flow. There were significant differences among these materials(p<0.05). 6. The correlation coefficient between Si loss and degradation layer depth (r=0.602, p〈0.05), Vicker's hardness number and maximum wear depth (r=0.501, p〈0.05) were relatively high. These results indicate that wear and hydrolytic degradation may be considered to be evaluation factors of composite resins and compomers.

VDRL 硝子板法 陰·陽性 血淸에 對한 Waaler-Rose 反應

梁學道,李楨浩,尹秉文,芮薰海,全東雨,金晋郁,玉東石 醫藥係社 1954 綜合 醫學 Vol.11 No.12

초음파 트랜스듀서용 PZT-고분자 1-3-0형 복합압전체의 유전 및 압전특성

양윤석,최헌일,사공건 東亞論叢 1999 東亞論叢 Vol.36 No.-

In this study, piezoelectric composites with 1-3-0 connectivity have been studied. A piezoelectric ceramic PZT prepared by the Wet-Dry Combination method is used as a filler phase in mixture of the third filler phase and Eccogel polymer matrix. The density and stiffness of 1-3-0 type composites were decreased with increasing the third phase.

뇌 해마의 복측 또는 배측 부분이 제거된 흰쥐 중격핵의 신경전달물질

양훈모,김종규,한영길,민영기 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

This study was planned to analyze amino acids in the septal nucleus of dorsal and ventral hippocampectomized rats by high performance liquid chromatography (HPLC). Male albino rats were used. Under chloral hydrate anesthesia (20%, 2 ml/kg body weight), a hole was made in each parietal bone about 4 mm lateral to the sagittal suture, and 4 mm rostral to the lambda suture. The cortical tissue on each side was removed and, through this opening, the hippocampal tissue anterodorsal and posteroventral to the hippocampal flexures on both sides was removed by aspiration. This preparation served as the dorsal and the ventral hippocampal animals, respectively. The cortical control animal received the same surgery short of hippocampectomy. The normal rats served as normal control animal. One day later, the animals were sacrificed by decapitation in the cold room. Two to three milligrams of tissue was obtained form the septal nucleus in one side of the brain. The tissue samples were homogenized and centrifuged. Then content of each amino acid was measured by HPLC form the brain tissue. The contents of aspartate and glutamate were decreased significantly more in the dorsal and ventral hippocampal groups than in the normal and the cortical control groups. The contents of glutamate were decreased significantly more in the ventral hippocampal group than in the dorsal hippocampal, while there were no significant differences between the two control groups. It is inferred form the above mentioned results that glutamate and aspartate may be used as excitatory transmitters in septal nucleus, and that the dorsal hippocampus may be facilitatory to the septal nucleus, but the ventral hippocampus would be inhibitory/facilitatory to the cortex.

개에서 17-ODYA가 신혈류량 자동 조절에 미치는 영향

양훈모,민영기,박중섭 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

17-ODYA is a potent inhibitor of the formation of 20-hydroxyeicosatetraenoic acid, epoxyeicosatrienoic acids and dihydroeicosatetraenoic acids by renal cortical microsomes. This study characterized the effects of intrarenal infusion of 17-ODYA on renal blood flow (RBF) and autoregulation of RBF. Seven dogs were prepared for the measurements of RBF, mean systemic and renal perfusion pressure. The renal perfusion pressure was able to be adjusted to desired levels by an inflatable supra-aortic occluder. Intrarenal infusion of 17-ODYA produced a diuresis and a natriuresis but no change in RBF. 17-ODYA had no effect on autoregulation of RBF: Both the plateau and steep portrions. of the autoregulatory curves were identical to control. Thesholds for lower limit of autoregulation were 72±4 and 72±5 mmHg during control and 17-ODYA and not significantly different from each other. These results suggest that endogenous cytochrome P-450 metabolites of AA do not influence RBF autoregulatory capacity in dogs.

수입소동맥 저항과 수출소동맥 저항의 상호 작용에 대한 연구

양훈모,민영기,이정범 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

It has generally been accepted that efferent arteriolar constriction increase GFR. However, it has also been shown in mathematical studies that increases in efferent arteriolar resistance beyond some extent causes GFR to decrease. The aim of the present study is to investigate the occurrence of physiological and/or pathophysiological conditions in which GFR is decreased by efferent constriction. The renal hemodynamic data obtained by several investigators were analyzed with a mathematical model of single nephron GFR (SNGFR). The glomerular model is based on a simulation of flow along a glomerular capillary with negligible resistance and uniform ultrafiltration coefficient (K_(f), 0.09 nl · sec^(-1) · mmHg^(-1)). The data analyzed are from rats which have been shown to achieve filtration pressure equilibrium(FPE) during filtration along the glomerular capillary. The hemodynamic states of rat kidney is characterized by a high K_(f), low RBF, and high vascular resistance. In these animals, inhibition of the renin-angiotensin system was associated with increases in GFR. This was mainly contributed to the increase in K_(f). Analysis with the present model has shown that lowered efferent arteriolar resistance during angiotensin inhibition augmented GFR response to K_(f). The analysis of the data obtained by manipulating efferent resistance during aortic constriction demonstrated the importance of pregiomerular resistance(R_(PRE)) related to efferent resistance (R_(E)). in this hemodynamic condition. The FPE which determines the sensitivity of SNGFR to SNBF and K_(f) is attained nearer the afferent end as R_(PRE) increases further: On the other hand, R_(PRE) had no effect on FPE. In conclusion, certain physiological manipulation affects R_(PRE) and K_(f) in such fashion that a decrease in R_(E) may have a beneficial effect on GFR and vice versa. This analysis indicates that it is not limited to theoretical importance; it demonstrated that in rat kidneys which have low flow and high afferent resistance the efferent arteriolar dilation can be associated with the promotion of GFR.

중이수술시 일측성 고삭신경 손상 후 미각변화

조양선,정유석,강수미,동헌종 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.2