Channel Capacity Analysis of DNA-based Molecular Communication with Length Encoding Mechanism

( Jialin Xie ),( Qiang Liu ),( Kun Yang ),( Lin Lin ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.8

The double helix structure of DNA makes it diverse, stable and can store information with high density, and these characteristics are consistent with the requirements of molecular communication for transport carriers. In this paper, a specific structure of molecular communication system based on DNA length coding is proposed. Transmitter (Tx) adopts the multi-layer golden foil design to control the release of DNA molecules of different lengths accurately, and receiver (Rx) adopts an effective and sensitive design of nanopore, and the biological information can be converted to the electric signal at Rx. The effect of some key factors, e.g., the length of time slot, transmission distance, the number of releasing molecules, the priori probability, on channel capacity is demonstrated exhaustively. Moreover, we also compare the transmission capacity of DNA-based molecular communication (DNA-MC) system and concentration-based molecular communication (MC) system under the same parameter setting, and the peak value of capacity of DNA-MC system can achieve 0.08 bps, while the capacity of MC system remains 0.025 bps. The simulation results show that DNA-MC system has obvious advantages over MC system in saving molecular resources and improving transmission stability.

Topology and start-up strategy for DC-DC transformers based on voltage balancing unit

Zhang, Jialin,Zha, Kunpeng,Tang, Xiaojun,Yang, Yuefeng,Yan, Hui,Wen, Fuyue,Shi, Mingming The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.7

As a core piece of equipment in DC distribution networks, DC solid-state transformers (DCSSTs) are attracting more and more attention in academia and industry. Due to the limitations in terms of the electrical stress of the switches, the input series output parallel (ISOP) structure is adopted in DCSSTs. This paper proposed an improved DCSST topology based on a voltage balancing unit (VBU). This topology has the advantages of higher power density, reduced weight, and cascaded number without compromising efficiency, cost, or reliability. The working modes, mathematical models, and control strategy are analyzed. In addition, a start-up strategy and a parameter design method are proposed in this paper. Simulation and experimental results verify the correctness and effectiveness of the proposed solution. The proposed DCSST is a practical scheme for the application of DC distribution networks.

Overexpression of TRIM44 mediates the NF-κB pathway to promote the progression of ovarian cancer

Yu Yang,Li ShiYing,Sun Jialin,Wang Yu,Xie LiangZhen,Guo Ying,Li Jia,Han FengJuan 한국유전학회 2024 Genes & Genomics Vol.46 No.6

Background Ovarian cancer (OC) is the second most commonly seen cancer in the US, and patients with OC are commonly diagnosed in the advanced stage. Research into the molecular mechanisms and potential therapeutic targets of OC is becoming increasingly urgent. In our study, we worked to discover the role of TRIM44 in OC development. Objective This study explored whether the overexpression of TRIM44 mediates the NF-kB pathway to promote the progression of OC. Methods A TRIM44 overexpression model was constructed in SKOV3 cells, and the proliferation ability of the cells was detected using the CCK-8 assay. The migration healing ability of cells was detected using cell scratch assay. Cell migration and invasion were detected using Transwell nesting. TUNEL was applied to detect apoptosis, and ELISA and western blot were used to detect the expression of NF-κB signaling pathway proteins. The pathological changes of the tumor tissues were observed using HE staining in a mouse ovarian cancer xenograft model. Immunofluorescence double staining, RT-PCR, and western blot were used to determine the expression of relevant factors in tumour tissues. Results TRIM44 overexpression promoted the proliferation, migration, and invasion of SKOV3 cells in vitro and inhibited apoptosis while enhancing the growth of tumours in vivo. TRIM44 regulated the NF-κB signaling pathway. Conclusions TRIM44 overexpression can regulate the NF-κB signaling pathway to promote the progression of OC, and TRIM44 may be a potential therapeutic target for OC. Background Ovarian cancer (OC) is the second most commonly seen cancer in the US, and patients with OC are commonly diagnosed in the advanced stage. Research into the molecular mechanisms and potential therapeutic targets of OC is becoming increasingly urgent. In our study, we worked to discover the role of TRIM44 in OC development. Objective This study explored whether the overexpression of TRIM44 mediates the NF-kB pathway to promote the progression of OC. Methods A TRIM44 overexpression model was constructed in SKOV3 cells, and the proliferation ability of the cells was detected using the CCK-8 assay. The migration healing ability of cells was detected using cell scratch assay. Cell migration and invasion were detected using Transwell nesting. TUNEL was applied to detect apoptosis, and ELISA and western blot were used to detect the expression of NF-κB signaling pathway proteins. The pathological changes of the tumor tissues were observed using HE staining in a mouse ovarian cancer xenograft model. Immunofluorescence double staining, RT-PCR, and western blot were used to determine the expression of relevant factors in tumour tissues. Results TRIM44 overexpression promoted the proliferation, migration, and invasion of SKOV3 cells in vitro and inhibited apoptosis while enhancing the growth of tumours in vivo. TRIM44 regulated the NF-κB signaling pathway. Conclusions TRIM44 overexpression can regulate the NF-κB signaling pathway to promote the progression of OC, and TRIM44 may be a potential therapeutic target for OC.

The Impact of Line Resistance on Load Sharing and An Improved Droop Control of DC Microgrid

CAO Wei,YANG Daopei,CAO Jialin,WANG Yongzhi,YAN Yuxing 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

This paper proposes an effective DC voltage and power sharing droop control structure for DC microgrid based on public bus voltage feedback signal. Firstly, the impact of the line resistance on power sharing among droop controlled distributed generators is analyzed and the reason why the line resistance cannot be ignored is presented. Then, an improved droop control is proposed. The simulation test based on MATLAB/Simulink was implemented to demonstrate the proposed approach.

Parasite-insecticide interactions: The impact of Nosema ceranae and fenpyroximate on honeybees (Apis mellifera)

Zheng Xing,Wang Xue,Yang Jialin,Peng Wenjun,Zhao Yazhou 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

Honeybees are susceptible to parasitic infections. As a consequence, beekeepers often apply pesticides in their hives. This approach will also affect the health of bees while eliminating parasites. We used Nosema ceranae (2 × 10 7 spores/mL), 0.002 % fenpyroximate, and the combination of N. ceranae-fenpyroximate to respectively treat newly emerged worker bees, to assess their survival curve, spore number and the expression levels of genes related to oxidative stress in the midguts. As a result, chronic exposure to N. ceranae and/or fenpyroximate significantly decreased honeybee survival. However, there were no different effects between the N. ceranae and fenpyroximate treatments (P > 0.05). Fenpyroximate inhibited the reproduction of N. ceranae in the early stage, but the number of N. ceranae increased gradually in the later stage (P < 0.01). Furthermore, the expression levels of genes related to oxidative stress were constantly changing with time under different treatments. In particular, the expression of the catalase gene was upregulated, and that of the ROS modulator 1-like gene and the superoxide dismutase gene was downregulated. In conclusion, this study indicated that the negative impact of N. ceranae and/or fenpyroximate on the health of honeybees caused the activation of their antioxidant system. Moreover, the redox status of the bees could increase their susceptibility and mortality.

A fluorescent probe for colorimetric detection of bisulfite and application in sugar and red wine

Haitao Chen,Xiaoming Wu,Jialin Wang,Hao Wang,Feiyan Tao,Shaoxiang Yang,Hongyu Tian,Yongguo Liu,Baoguo Sun 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4

A new fluorescent probe made from (E)-2-(benzo[d]thiazol-2-yl)-3-(6-hydroxynaphthalen-2-yl) acrylonitrile(Probe 1) was synthesized for the determination ofbisulfite concentrations in real food samples (red wine andsugar). Adding bisulfite to a Probe 1 solution caused amarked decrease in fluorescence intensity and a visualcolor change from yellow to light yellow. This distinctcolor response indicates that Probe 1 could be used as avisual sensor for bisulfite. Probe 1 can detect bisulfitequantitatively in the range 0–400 lM with a detection limitof 0.10 lM. This makes Probe 1 a convenient signalinginstrument for determining bisulfite levels in sugar and redwine samples.

Effect of proton irradiation on interfacial and electrical performance of N+Np+ InP/InGaAs hetero-junction

Zhang Chen,Su Yongbo,Mei Bo,Yang Feng,Zhang Jialin,Yun Huanqing,Liu Bo,Sun Yi,Zhang Haiming,Jin Zhi,Zhong Yinghui 한국물리학회 2023 Current Applied Physics Vol.48 No.-

InP-based hetero-junction bipolar transistors (HBTs) are attractive for various millimeter-wave and terahertz electronics due to their ultrahigh frequency performance. Therefore, the study of their irradiation reliability is extremely urgent. In this work, 2 MeV proton irradiation experiment has been carried out on self-fabricated N+Np+ InP/InGaAs hetero-junction. The degradation mechanisms have systematically been studied after exposure to protons of 5 × 1013 H+/cm2. The voltage range of recombination current at low forward bias has been largely expanded from 0-0.2 V to 0–0.6 V. Furthermore, the interface states properties have been characterized by utilizing the frequency-dependent conductance technique, the interface states density (Dit) has increased from approximately 9.02 × 1012 cm-2 eV-1–1.99 × 1013 cm-2 eV-1 to 9.51 × 1012 cm-2 eV-1–2.00 × 1013 cm-2 eV-1 after irradiation. The study would be great significance for understanding the evolution of proton irradiation on relative devices.

Evaluation of the YOLO models for discrimination of the alfalfa pollinating bee species

Zhang Chuan-Jie,Liu Teng,Wang Jinxu,Zhai Danlan,Zhang Youxin,Gao Yang,Wu Hui-Zhen,Yu Jialin,Chen Min 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

Identifying insect pollinators and their roles in mediating pollen flow is critical to understand the potential gene flow risks of insect pollination-dependent crop species, such as alfalfa. This study was conducted to evaluate and compare the feasibility of You Only Look Once (YOLO) version 3 (YOLOv3), YOLOv5, YOLOv7, and YOLO Representation (YOLOR) to discriminate the three most common alfalfa pollinating bee species, including honeybee, bumblebee, and leafcutting bee. The metrics comparison results showed YOLOv3 and YOLOv5 out performed YOLOv7 and YOLOR regarding model precision, recall, F 1 score, and mAP50 values. YOLOv3 and YOLOv5 could successfully discriminate the three different bee species with an accuracy of almost 100% (99.9%, 99.8%, and 100% accuracy for honeybee, bumblebee, and leafcutting bee for the two models, respectively). Comparatively, YOLOv7 could discriminate honeybee with an accuracy of 95% but was more likely to mistakenly discriminate bumblebee and leafcutting bee due to the relatively lower discriminating accuracy (87.3% and 66.2%, respectively). While the values of determined parameters for YOLOR were lower than YOLOv3 and YOLOv5, the higher precision (0.99680) along with recall (0.98721), F 1 (0.99198), mAP50 (0.99323), and mAP50-100 (0.89076) values indicate that this model could be able to obtain a favorable performance in discriminating the three bee species. In summary, the proposed method in this study has the potential for identifying the alfalfa pollinating bee species, studying the bees’ flower-visiting behaviors, evaluating the risks of insect-mediated pollen flow, and thus contributing to the management of genetically engineered (GE) alfalfa transgene flow.

A randomized controlled trial to compare the efficacy of regenerated and non-regenerated oxidized cellulose gauze for the secondary treatment of local bleeding in patients undergoing hepatic resection

Chengshuo Zhang,Dazhi Fu,Fengshan Wang,Xinping Zhong,Lei Yang,Gang Wu,Baifeng Li,Jialin Zhang 대한외과학회 2021 Annals of Surgical Treatment and Research(ASRT) Vol.100 No.4

Purpose: Oxidized cellulose is available in many forms, but manufactured using either a regenerated or non-regenerated process. In this study, we evaluated the effects of 2 different hemostatic agents for the treatment of local bleeding in patients undergoing hepatic resection. Methods: This was a monocentric, parallel-group, randomized, and controlled clinical trial to compare oxidized regenerated cellulose gauze (ORCG) with oxidized non-regenerated cellulose gauze (ONRCG) in patients undergoing hepatectomy. The primary endpoint was the time to hemostasis at the target bleeding site. The secondary endpoints were the postoperative drainage volume on the first 2 days after surgery and the hospital stay. Results: There was no significant difference between the ORCG and ONRCG groups in time to hemostasis from column analysis (238.8 ± 121.6 seconds vs. 193.7 ± 85.3 seconds, P = 0.068), and there were no differences in the rates of hemostatic success between the 2 groups at 120 seconds (18.4% vs. 24.3%; odds ratio [OR], 0.703; 95% confidence interval [CI], 0.231–2.136) and 300 seconds (71.1% vs. 89.2%; OR, 0.298; 95% CI, 0.085–1.041). However, the ONRCG group was superior to the ORCG group in hemostasis according to the survival analysis (log-rank test, P = 0.044). Moreover, there were also no significant differences between the 2 groups in postoperative drainage volume on the first 2 days (P = 0.436, P = 0.381) and hospital stay (P = 0.537, P = 0.200). Conclusion: ONRCG was not inferior to ORCG as a hemostatic agent in patients undergoing liver resection.

Identification and Characterization of a New Alkaline SGNH Hydrolase from a Thermophilic Bacterium Bacillus sp. K91

( Tingting Yu ),( Junmei Ding ),( Qingxia Zheng ),( Nanyu Han ),( Jialin Yu ),( Yunjuan Yang ),( Junjun Li ),( Yuelin Mu ),( Qian Wu ),( Zunxi Huang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4

est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3). The enzyme activity was optimal at 60°C and pH 9.0, and displayed esterase activity towards esters with short-chain acyl esters (C2-C6). A structural model of Est19 was constructed using phospholipase A1 from Streptomyces albidoflavus NA297 as a template. The structure showed an α/β-hydrolase fold and indicated the presence of the typical catalytic triad Ser49-Asp227-His230, which were further investigated by site-directed mutagenesis. To the best of our knowledge, Est19 is a new member of the SGNH hydrolase family identified from thermophiles, which may be applicable in the industrial production of semisynthetic β-lactam antibiotics after modification.