Scalded Skin of Rat Treated by Using Fibrin Glue Combined with Allogeneic Bone Marrow Mesenchymal Stem Cells

( Yadong Yang ),( Wenyuan Zhang ),( Ying Li ),( Guojian Fang ),( Keji Zhang ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.3

Background: It is difficult to achieve satisfactory results with the traditional treatment of large-area skin defects and deep burns. Objective: To test the treatment effect of an active dressing film made of a mixture of fibrin glue and bone marrow mesenchymal stem cells (BMSCs) for repairing burn wounds on the skin of rats. Methods: Two scald wounds were made on the back of each rat. A total of 30 scald wounds were randomly divided into 3 groups, with 10 wounds in each group. In the experimental treatment group, the scald wounds were covered with the fibrin glue and BMSC mixture. The wounds of the experimental control group were covered with fibrin glue only. No intervention was administered to the blank control group. Thirty days after treatment, pathological sections were cut from the scalded local tissues of all rats from the 3 groups and observed with a microscope. Results: The speed of scald wound healing in the experimental treatment group was faster than the other 2 groups. In the experimental treatment group, histopathological analysis revealed that the sebaceous glands showed obviously proliferous at the edge of the new tissue and gradually extended to the deep dermal layer of the new tissue. Conclusion: BMSCs may have an active role in promoting skin tissue repair and generating skin appendages. Allogeneic BMSCs mixed with fibrin glue can contribute to the quick formation of a film-like gel over the scald wounds, which might be of significance for emergency treatment and skin-grafting operations. (Ann Dermatol 26(3) 289∼295, 2014)

Additive/subtractive hybrid manufacturing of 316L stainless steel powder: Densification, microhardness and residual stress

Yuying Yang,Yadong Gong,Shuoshuo Qu,Bo Xin,Yunchao Xu,Yang Qi 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.12

316L stainless steel specimens were manufactured by additive/subtractive hybrid manufacturing (ASHM). The densification level, microstructure, microhardness and residual stress characterization in the different zones of the part were investigated. The density was determined by the Archimedes method, and the density measurement was divided into three regions, namely, the bottom, middle and top zones. The results show that the middle zone has a much smoother melting surface and that a relative density of nearly 100 % was achieved for the part in this study. The hardness profiles at room temperature, along the width and height directions of the cross-section of the top and bottom zones, were also studied. The residual stress was evaluated by X-ray diffraction (XRD) for the 316L SS specimen fabricated by the ASHM process, and it was compared with a specimen manufactured by the additive laser directed energy deposition (DED) process. The results show that the top and bottom zones exhibit tensile stress, and compressive stress occurs in the middle area. Moreover, the residual stress of ASHM shows a slightly smaller trend than that of the simplex DED due to the stress relaxation of the subsequent subtractive milling. These results may offer guidance for ASHM-fabricated 316L parts.

Robust Multi-Layer Hierarchical Model for Digit Character Recognition

Yang, Jie,Sun, Yadong,Zhang, Liangjun,Zhang, Qingnian The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.2

Although digit character recognition has got a significant improvement in recent years, it is still challenging to achieve satisfied result if the data contains an amount of distracting factors. This paper proposes a novel digit character recognition approach using a multi-layer hierarchical model, Hybrid Restricted Boltzmann Machines (HRBMs), which allows the learning architecture to be robust to background distracting factors. The insight behind the proposed model is that useful high-level features appear more frequently than distracting factors during learning, thus the high-level features can be decompose into hybrid hierarchical structures by using only small label information. In order to extract robust and compact features, a stochastic 0-1 layer is employed, which enables the model's hidden nodes to independently capture the useful character features during training. Experiments on the variations of Mixed National Institute of Standards and Technology (MNIST) dataset show that improvements of the multi-layer hierarchical model can be achieved by the proposed method. Finally, the paper shows the proposed technique which is used in a real-world application, where it is able to identify digit characters under various complex background images.

Cenozoic tectonic evolution in the western Qaidam Basin inferred from subsurface data

Yadong Wang,Junsheng Nie,Tao Zhang,Guoqiang Sun,Xin Yang,Yuhu Liu,Xingwang Liu 한국지질과학협의회 2010 Geosciences Journal Vol.14 No.4

It is an agreement that collision of Indian and Asian plates causes uplift of the Tibet Plateau. However, great controversy exists about uplifting history and mechanisms of the Tibetan Plateau. Uplift history of the Tibetan Plateau has been well recorded in the Qaidam Basin, located in the northern margin of the Tibet Plateau. In this paper, we recognize and classify faults recorded by subsurface seismic data in the western Qaidam Basin. In addition, we reconstruct the Cenozoic deformation history of the Qaidam Basin based on balanced section of 5 seismic profiles. The results indicates that 1) Faults in the western Qaidam Basin can be classified as growth faults and non-growth faults, and the growth faults could be divided into three subcategories. 2) According to timing and manner of fault activities, faults and strata in the western Qaidam Basin could be divided into two structural layers: the lower (Lulehe Fm-Xia Youshashan Fm) and the upper (Shang Youshashan Fm-Qigequan Fm) layer. 3) The western Qaidam Basin has experienced two intensive tectonic deformations: the first phase occurs at 43.8−22 Ma (Middle Eocene−Early Miocene), which reached peak at 31.5 Ma (Early Oligocene); the second phase occurred between 14.9 and 0 Ma (Middle Miocene−Present), and the second phase is stronger than the first phase. Recognizing early fault activities confirm previous results that northern Tibet has sensed collision between the India and the Asia shortly after the collision. However, our results here emphasize that the northern Tibet has experienced another phase of shortening and uplift in the late Neogene. It was the two-stage tectonic activities that work together to produce the current Tibetan Plateau.

Depth tracking of occluded ships based on SIFT feature matching

Yadong Liu,Yuesheng Liu,Ziyang Zhong,Yang Chen,Jinfeng Xia,Yunjie Chen 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.4

Multi-target tracking based on the detector is a very hot and important research topic in target tracking. It mainly includes two closely related processes, namely target detection and target tracking. Where target detection is responsible for detecting the exact position of the target, while target tracking monitors the temporal and spatial changes of the target. With the improvement of the detector, the tracking performance has reached a new level. The problem that always exists in the research of target tracking is the problem that occurs again after the target is occluded during tracking. Based on this question, this paper proposes a DeepSORT model based on SIFT features to improve ship tracking. Unlike previous feature extraction networks, SIFT algorithm does not require the characteristics of pre-training learning objectives and can be used in ship tracking quickly. At the same time, we improve and test the matching method of our model to find a balance between tracking accuracy and tracking speed. Experiments show that the model can get more ideal results.

Robust Multi-Layer Hierarchical Model for Digit Character Recognition

Jie Yang,Yadong Sun,Liangjun Zhang,Qingnian Zhang 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.2

Although digit character recognition has got a significant improvement in recent years, it is still challenging to achieve satisfied result if the data contains an amount of distracting factors. This paper proposes a novel digit character recognition approach using a multi-layer hierarchical model, Hybrid Restricted Boltzmann Machines (HRBMs), which allows the learning architecture to be robust to background distracting factors. The insight behind the proposed model is that useful high-level features appear more frequently than distracting factors during learning, thus the high-level features can be decompose into hybrid hierarchical structures by using only small label information. In order to extract robust and compact features, a stochastic 0-1 layer is employed, which enables the model’s hidden nodes to independently capture the useful character features during training. Experiments on the variations of Mixed National Institute of Standards and Technology (MNIST) dataset show that improvements of the multi-layer hierarchical model can be achieved by the proposed method. Finally, the paper shows the proposed technique which is used in a real-world application, where it is able to identify digit characters under various complex background images.

Influence of Molecular Weight on the Dielectric and Energy Storage Properties of Poly(vinylidene fluoride)

Yuetao Zhao,Wenyao Yang,Yujiu Zhou,Yan Chen,Yajie Yang,Jianhua Xu,Yadong Jiang 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6

The molecular weight shows great influence on dielectricand energy storage performance of poly(vinylidenefluoride) (PVDF) films and related devices. In this letter,the influences of molecular weight on the dielectric andenergy storage properties of PVDF films were studied. Ithas been found that, under a 1000 kV/cm electric field,the low-molecular-weight PVDF film presents a muchhigher energy storage efficiency as high as 80.10%,nearly three times as much as the high-molecular-weightPVDF film. Moreover, the low-molecular-weight PVDFfilm also shows high resistivity, representing an order ofmagnitude improvement over the high-molecular-weightPVDF film, which is more desirable and promising forhigh performance pulse discharge capacitor application.

Degradation of 1,4-dioxane by Newly Isolated Acinetobacter sp. M21 with Molasses as the Auxiliary Substrate

Peng Wang,Tingchen Cui,Yadong Yang,Jialu Li,Yaoming Su,Na Liu,Hong-Mei Li 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.3

The elimination of 1,4-dioxane (dioxane), a persistent organic pollutant, is a great challenge owing to its high hydrophilicity and chemical stability. Cometabolic bioremediation technology is an effective approach to remove many organic pollutants. Because of its eco-friendly and inexpensive properties, molasses is widely used as an auxiliary biomaterial to clean up compound-contaminated sites. In this study, a newly isolated bacterium Acinetobacter sp. M21 could effectively remove dioxane using molasses without any apparent lag phase. Under the optimized molasses dosage of 0.3%, M21 could remove 500 mg/L dioxane by 60.0 ± 2.8% within 20 days with a maximum dioxane degradation rate of 1.3 ± 0.2 mg-dioxane/L/h in the first day, and exhibited extraordinary dioxane tolerance up to 1,000 mg/L, while so high dose of dioxane negatively affected the cell growth. The degradation pathway of dioxane was also determined, and was supported by the detection of 2-hydroxyethoxyacetic acid as the key metabolite of dioxane. High level degradation activity of M21 to 20 mg/L dioxane was maintained over a variable of pH (5-11), temperatures (15-45°C), and salinities (up to 8%, as NaCl wt). This is the first report linking the cometabolism of dioxane and molasses by Acinetobacter sp. M21, a bacterium that shows great potential for field dioxane bioremediation.

Differential Hrd1 Expression and B-Cell Accumulation in Eosinophilic and Non-eosinophilic Chronic Rhinosinusitis With Nasal Polyps

Kun Chen,Miaomiao Han,Mengyao Tang,Yadong Xie,Yuting Lai,Xianting Hu,Jia Zhang,Jun Yang,Huabin Li 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.6

Purpose: Hrd1 has recently emerged as a critical regulator of B-cells in autoimmune diseases. However, its role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) remains largely unexplored. This study aimed to examine Hrd1 expression and B-cell accumulation and their possible roles in CRSwNP. Methods: Quantitative real-time polymerase chain reaction, immunohistochemistry, enzyme-linked immunosorbent assay and Western blotting were used to assess gene and protein expression in nasal tissue extracts. Cells isolated from nasal tissues and peripheral blood mononuclear cells were characterized by flow cytometry. Local antibody production was measured in tissue extracts with a Bio-Plex assay. Additionally, changes in Hrd1 expression in response to specific inflammatory stimuli were measured in cultured dispersed polyp cells. Results: Nasal polyps (NPs) from patients with eosinophilic CRSwNP (ECRS) had increased levels of Hrd1, B-cells and plasma cells compared with NPs from patients with non-eosinophilic CRSwNP (non-ECRS) or other control subjects (P < 0.05). The average Hrd1 levels in B-cells in NPs from ECRS patients were significantly higher than those from non-ECRS patients and control subjects (P < 0.05). NPs also contained significantly increased levels of several antibody isotypes compared with normal controls (P < 0.05). Interestingly, Hrd1 expression in cultured polyp cells from ECRS patients, but not non-ECRS patients, was significantly increased by interleukin-1β, lipopolysaccharide and Poly(I:C) stimulation, and inhibited by dexamethasone treatment (P < 0.05). Conclusions: Differential Hrd1 expression and B-cell accumulation between the ECRS and non-ECRS subsets suggests that they can exhibit distinct pathogenic mechanisms and play important roles in NP.

Association between perioperative glucocorticoids and cancer metastasis and survival in patients undergoing radical cystectomy for urothelial carcinoma of the bladder: A single-center retrospective study

Shiyu Mao,Yuan Wu,Ruiliang Wang,Yadong Guo,Jing Yuan,Wentao Zhang,Junfeng Zhang,Yang Yan,Xudong Yao 대한비뇨의학회 2020 Investigative and Clinical Urology Vol.61 No.4

Purpose: Perioperative glucocorticoids have the potential to increase the risk of tumor metastasis. However, the relationship between perioperative glucocorticoids and oncologic outcomes remains controversial. The present study was undertaken to evaluate the association of perioperative glucocorticoids with clinicopathologic outcomes following radical cystectomy (RC). Materials and Methods: We screened and included 185 patients who underwent radical surgery for bladder cancer in our center between 2009 and 2018. The Kaplan–Meier method was applied, and a log-rank test was used to estimate differences in metastasis-free survival (MFS) and overall survival (OS) between the groups. Multivariate Cox proportional hazards regression models were used to analyze any association of glucocorticoids with clinical outcomes. Results: A total of 76 (41.1%) patients received perioperative glucocorticoids. Median postoperative follow-up was 2.0 years. Kaplan–Meier survival curve indicated that the glucocorticoids group was significantly associated with increased distant MFS (p=0.008) but not with OS. In the multivariate analysis, no significant differences were observed for MFS between the groups. Interestingly, when the variable of blood transfusion was excluded from the multivariate analysis model, we found that patients receiving glucocorticoids were independently associated with worse MFS (hazard ratio, 1.790; p=0.030). Furthermore, the partial correlation analysis showed a significant positive correlation between perioperative glucocorticoids and blood transfusion (r=0.604, p<0.001). In the nontransfusion subgroup, propensity score matching showed that patients receiving glucocorticoids had a higher risk of distant metastasis. Conclusions: Perioperative glucocorticoids were associated with a higher rate of distant metastasis in patients undergoing RC for bladder cancer.