Effects of Insect Hormones on the Replication of Nucleopolyhedrovirus

( Ya Jing Zhou ),( Yong Zhu Yi ),( Qing Li Xiao ),( Zhi Fang Zhang ),( Jia Lu He ),( Yuan Xing Zhang ) 한국잠사학회 2002 International Journal of Industrial Entomology Vol.4 No.2

CbCOR15, A Cold-Regulated Gene from Alpine Chorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco

Jing Si,Jian-hui Wang,Li-jing Zhang,Hua Zhang,Ya-jie Liu,Li-zhe An 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6

Rapid amplification of cDNA ends was performed to isolate cold-regulated CbCOR15 (EF208112) from Chorispora bungeana. This alpine species is distributed in subnival areas. Transcripts were detected in the leaves, but not the roots, of plants acclimated to cold temperatures. Expression was induced at high levels at both 4°C and −4°C. In comparing its deduced protein sequence to that of AtCOR15a (cold-regulated 15a in Arabidopsis thaliana), the N terminus had less homology than the C terminus while still containing a region analogous to the chloroplast-targeted signal peptide of AtCOR15a. We also introduced CbCOR15, with the CaMV 35S promoter, into tobacco. Second-generation (T1) plants had significantly increased tolerance to chilling, as determined by their electrolyte leakage, chlorophyll content, and relative water content. Further freezing-stress experiments showed that the tolerance of transgenic lines was significantly greater than that of the nontransgenics. Although the degree of chilling and freezing tolerance in the transgenic plants was not directly correlated with the accumulated levels of CbCOR15, we could conclude that this gene confers cold tolerance.

Structural and photoelectrochemical characterization of TiO2 nanowire/nanotube electrodes by electrochemical etching

Jing Ya,Li An,Zhifeng Liu,Lei E,Wei Zhao,Dan Zhao,Chengcheng Liu 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.6

TiO2 nanowire/nanotube electrodes were synthesized by anodization of titanium foils in ethylene glycol solution containing 0.5 wt% NH4F and 1 wt% water at 60 V for 6 h. The microstructure and morphology of the asprepared electrodes were investigated by XRD and SEM. A possible formation mechanism and oxidation parameters of nanocomposite structure were discussed. The relationship between structural characteristics of TiO2 nanowire/nanotube electrodes and its photoelectrochemical characterization were evaluated by electrochemical analyzer and photocatalytic degradation of methylene blue (MB) solution. Furthermore, these TiO2 nanowire/nanotube electrodes promoted the photoelectrochemical characterization due to the larger surface areas, enhanced light harvesting and electron transport rate. The results show that photocurrent density of 1.44mA/cm2 and photocatalytic degradation of 95.51% was achieved for TiO2 nanowire/nanotube electrodes, which were 0.55mA/cm2 and 20.52% higher than the TiO2 nanotube electrodes under a similar condition, respectively.

Transcriptome sequencing reveals non-coding RNAs respond to porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in Kele piglets

( Jing Zhang ),( Chunping Zhao ),( Min Yao ),( Jing Qi ),( Ya Tan ),( Kaizhi Shi ),( Jing Wang ),( Sixuan Zhou ),( Zhixin Li ) 한국축산학회 2024 한국축산학회지 Vol.66 No.4

Co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and Haemophilus parasuis (HPS) has severely restricted the healthy development of pig breeding. Exploring disease resistance of non-coding RNAs in pigs co-infected with PRRSV and HPS is therefore critical to complement and elucidate the molecular mechanisms of disease resistance in Kele piglets and to innovate the use of local pig germplasm resources in China. RNA-seq of lungs from Kele piglets with single-infection of PRRSV or HPS and co-infection of both pathogens was performed. Two hundred and twenty-five differentially expressed long non-coding RNAs (DElncRNAs) and 30 DEmicroRNAs (DEmiRNAs) were identified and characterized in the PRRSV and HPS co-infection (PRRSV-HPS) group. Compared with the single-infection groups, 146 unique DElncRNAs, 17 unique DEmiRNAs, and 206 target differentially expressed genes (DEGs) were identified in the PRRSV-HPS group. The expression patterns of 20 DEmiRNAs and DElncRNAs confirmed by real-time quantitative polymerase chain reaction (RT-qPCR) were consistent with those determined by high-throughput sequencing. In the PRRSV-HPS group, the target DEGs were enriched in eight immune Gene Ontology terms relating to two unique DEmiRNAs and 16 DElncRNAs, and the unique target DEGs participated the host immune response to pathogens infection by affecting 15 immune-related Kyoto Encyclopedia of Genes and Genomes enrichment pathways. Notably, competitive endogenous RNA (ceRNA) networks of different groups were constructed, and the ssc-miR-671-5p miRNA was validated as a potential regulatory factor to regulate DTX4 and AEBP1 genes to achieve innate antiviral effects and inhibit pulmonary fibrosis by dual-luciferase reporter assays. These results provided insight into further study on the molecular mechanisms of resistance to PRRSV and HPS co-infection in Kele piglets.

Transcriptome profiling identifies immune response genes against porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in the lungs of piglets

Jing Zhang,Jing Wang,Xiong Zhang,Chunping Zhao,Sixuan Zhou,Chunlin Du,Ya Tan,Yu Zhang,Kaizhi Shi 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

Background: Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. Objectives: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. Methods: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. Results: All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analysis showed that 922 DEGs in co-challenged pigs were more than in the HPS group (709 DEGs) and the PRRSV group (676 DEGs). Eleven DEGs validated by qRT-PCR were consistent with the RNA sequencing results. Eleven common Kyoto Encyclopedia of Genes and Genomes pathways related to infection and immune were found in single-infected and co-challenged pigs, including autophagy, cytokine-cytokine receptor interaction, and antigen processing and presentation, involving different DEGs. A model of immune response to infection with PRRSV and HPS was predicted among the DEGs in the co-challenged pigs. Dual oxidase 1 (DUOX1) and interleukin-21 (IL21) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. Conclusions: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.

FEM Analysis for Laser Bending Process of DP980 Steel Sheet

Ya Jing Zhang,김종봉,송정한,이근안,이혜진,이낙규 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

Laser bending of metal sheet is a complex process that is achieved by introducing thermal stress into a metal sheet through irradiationusing a defocused laser beam. In a single scanning process, it is important to know the effects of the engineering parameters on thefinal bending angle, such as the laser power, laser beam diameter, and scanning speed. Furthermore, it is important to investigatethe effect of the scanning direction in a multiple scanning process. In this paper, FEM analyses for a laser bending process of DP980high strength steel sheet under different laser powers, scanning speeds, line energies, and scanning directions are performed toanalyze the effects of the engineering parameters and the scanning direction on the final bending angle. The results indicate that thefinal bending angle of a DP980 steel sheet increases in a linear increase fashion with the increase of the line energy in the singlescanning process; it also increases when the scanning direction is changed time by time in the multiple scanning process.

결혼이주여성의 공적 지원서비스 초기 이용 유형과 관련 요인

딩징야(Jing-ya Ding),진미정(Meejung Chin) 가정과삶의질학회 2021 가정과삶의질연구 Vol.39 No.4

Comprehensive Expression Analysis Suggests Functional Overlapping of Human FOX Transcription Factors in Cancer

Zhang, Ya-Li,Sun, Feng-Ting,Zhang, Ze,Chen, Xiao-Xu,Liu, Ai-Xiang,Pan, Jing-Jing,Peng, Fei,Zhou, Shuai,Sun, Li-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Forkhead-box (FOX) transcription factors comprise a large gene family that contains more than 50 members in man. Extensive studies have revealed that they not only have functions in control of growth and development, but also play important roles in different diseases, especially in cancer. However, biological functions for most of the members in the FOX family remain unknown. In the present study, the expression of 39 FOX genes in 48 kinds of cancer was mined from the Gene Expression Atlas database of European Bioinformatics Institute. The analysis results showed that some FOX genes demonstrate overlapping expression in various cancers, which suggests particular biological functions. The pleiotropic features of the FOX genes make them excellent candidates in efforts aimed to give medical treatment for cancers at the genetic level. The results also indicated that different FOX genes may have the synergy or antagonistics effects in the same cancers. The study provides clues for further functional analysis of FOX genes, especially for the pleiotropic biological functions and crosstalk of FOX genes in human cancers.

성장마인드셋이 자기효능감에 영향을 미침에 있어서 그릿의 매개효과: 중국 하이난성 무용전공 대학생을 대상으로

진정아(Jing Ya Chen),증빈(Bin Zeng),고영춘(Young Chun Ko) 학습자중심교과교육학회 2024 학습자중심교과교육연구 Vol.24 No.17

목적 본 연구의 목적은 무용전공 대학생의 자기효능감을 높이기 위해 성장마인드셋, 자기효능감 및 그릿 간의 관계를 밝히고, 성장마인드셋이 자기효능감에 미치는 영향에서 그릿의 매개효과를 검증하는 것이다. 방법 연구대상은 중국 하이난성 Q 사범대학교 무용전공 대학생인 239명을 대상으로 하였고, SPSS 25.0 프로그램을 사용하여 신뢰도, 평균차이, 상관관계 및 회귀의 분석을 하였다. 그리고 성장마인드셋과 자기효능감 간의 그릿의 매개효과를 알기 위하여, 성장마인드셋에 따른 그릿의 단순회귀분석을 하였고, 성장마인드셋에 따른 자기효능감의 단순회귀분석을 하였고, 그리고 성장마인드셋과 그릿에 따른 자기효능감의 다중회귀분석을 하였다. 결과 성장마인드셋, 그릿 및 자기효능감 사이에는 서로 간에 정(+)적인 상관관계를 나타냈다. 성장마인드셋의 각 하위변수인 지능 변화신념과 성격 변화신념은 자기효능감의 전체 변량의 58%를 설명하였다. 그릿이 자기효능감에 미치는 영향을 분석한 결과, 그릿은 자기효능감의 전체 변량의 50%를 설명하였다. 지능 변화신념, 성격 변화신념 및 그릿의 예측변수가 자기효능감의 전체 변량의 68%를 설명하였다. 지능 변화신념과 성격 변화신념이 그릿에 미치는 영향을 분석하였는데, 이때 지능 변화신념과 성격 변화신념은 그릿의 전체 변량의 36%를 설명하였다. 무용전공 대학생의 성장마인드셋이 자기효능감에 영향을 미침에 있어서 그릿에 의한 부분적인 매개효과가 있었다. 결론 본 연구에서는 성장마인드셋이 그릿과 자기효능감을 높이는 원인변수라는 것을 확인하였고, 성장마인드셋이 자기효능감에 정적인 영향을 줌에 있어서 그릿이 부분매개 함을 확인하였다. 그래서 대학생의 자기효능감을 함양하기 위해서는 성장마인드셋과 그릿을 증진 시켜야 한다. 한편 사회적 지지와 자기표현의 각 변수는 무용전공 대학생의 자기효능감에 영향을 줄 것이다. 그래서 추후 연구에서는 사회적 지지와 자기표현의 변수들에 따른 무용전공 대학생에 관한 자기효능감의 연구도 수행할 필요가 있을 것이다. Objectives The purpose of this study is to reveal the structural relationship among growth mindset, self-efficacy, and grit to increase self-efficacy of university students majoring in dance, and to verify the mediating effect of grit in the effect of growth mindset on self-efficacy. Methods The subjects of this study are 239 university students majoring in dance at Q Normal University in Hainan of China, and reliability, average differences, correlations, and regression analysis are conducted using the SPSS 25.0 program. And to find out the mediating effect of grit between the growth mindset and self-efficacy, simple regression analysis of grit according to the growth mindset is performed, simple regression analysis of self-efficacy according to the growth mindset, and multiple regression analysis of self-efficacy according to the growth mindset and grit is performed. Results There is a positive(+) correlation between the growth mindset, grit, and self-efficacy. The effect of intelligence change beliefs and personality change beliefs, which are sub-variables of the growth mindset, on self-efficacy explains 58% of the total variance of self-efficacy. As a result of analyzing the effect of grit on self-efficacy, grit explains 50% of the total variance of self-efficacy. Predictors of 'sub-variables of the growth mindset' and grit explain 68% of the total variance of self-efficacy. The effect of intelligence change beliefs and personality change beliefs, which are sub-variables of the growth mindset, on grit is analyzed, and the growth mindset explains 36% of the total variance of grit. There is a partial mediating effect by grit in influence of growth mindset on self-efficacy of university students majoring in dance. Conclusions In this study, it is confirmed that the growth mindset and grit is a causal variable that increases self-efficacy, and the grit partially mediates the positive effect of the growth mindset on self-efficacy. Therefore, in order to cultivate the self-efficacy of university students, it is necessary to promote the growth mindset and grit. On the other hand, each variable of social support and self-expression will affect the self-efficacy of university students majoring in dance. And so, it will be necessary to conduct a study on the self-efficacy of university students majoring in dance according to the variables of social support and self-expression in future studies.

ABO Blood Group, Epstein-Barr virus Infection and Prognosis of Patients with Non-metastatic Nasopharyngeal Carcinoma

Zhang, Ya-Xiong,Kang, Shi-Yang,Chen, Gang,Fang, Wen-Feng,Wu, Xuan,You, Hua-Jing,He, Da-Cheng,Cao, Ya-Lin,Liang, Wen-Hua,Zhang, Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Background: A prior study showed blood type A/AB to be associated with an increased risk of nasopharyngeal carcinoma (NPC) compared to subjects with blood type O. However, the relationship between ABO blood groups and prognosis of NPC patients is still questionable. In addition, whether Epstein-Barr virus (EBV) infection is associated with prognosis of NPC patients with different ABO blood groups is unclear. Materials and Methods: We conducted univariate and multivariable Cox regression analyses based on a consecutive cohort of 1,601 patients to investigate the above issues. Results: There was no significant difference in overall survival (OS) between different ABO blood groups (p=0.629), neither between A vs. non-A blood groups (p=0.895) nor AB vs. non-AB blood group (p=0.309) in univariate analyses and after adjusting for other factors. Interaction tests revealed that high immunoglobulin A against Epstein-Barr virus viral capsid antigen (VcA-IgA) level was associated with a favorable prognosis in male patients with UICC stage II disease who had an A blood type (p=0.008), compared with those with non-A blood type. In addition, male patients with an A blood group with a high blood lymphocyte level showeda tendency towards better survival in UICC stage III (p=0.096). Conclusions: ABO blood group status is not associated with the prognosis of patients with NPC. Additionally, blood group A male NPC patients with high VcA-IgA level or high blood lymphocyte counts might be correlated with a favorable prognosis in UICC stage II or III, respectively.