Phenolic Compounds from Arundina graminifolia and Their Anti-Tobacco Mosaic Virus Activity

Xuemei Gao,Liying Yang,Yanqiong Shen,Lidang Shu,Xuemei Li,Qiu-Fen Hu 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

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Efficacy and safety of herbal medicine (Binafuxi granules) for the common cold with fever: A multicenter, randomized, double-blind, placebo-controlled, phase II clinical trial

Liu Xuemei,Min Jie,She Bin,Chen Yang,Li Jun,Huang Lei,Chen Ju,Luo Ai,Mei Yang,Li Ting,Wu Yanqing,Chen Daohong,Hongli Zhong,Liu Wei,Mao Bing,Jiang Hongli 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.3

Background: Binafuxi granules are a traditional Uighur medicine (TUM) for treating the common cold with fever. However, high-quality clinical studies supporting its efficacy and safety are lacking. Methods: In this multicenter, randomized, double-blind, placebo-controlled, phase II clinical trial, patients with common cold and fever were randomly assigned to a high-dose group, low-dose group, and placebo group in a 1:1:1 ratio. Outcomes were time to fever relief, time to fever clearance, proportion of afebrile patients, time to symptom disappearance, rate of symptom disappearance, effective rate, emergency drug usage and safety assessment. Results: A total of 235 patients were recruited. Of these, 234 were included in the full analysis set (FAS), and 217 were included in the per-protocol set (PPS). In the FAS analysis, the median time to fever relief was 6.00 h, 5.54 h and 10.65 h (P = 0.31) in the high-dose group, low-dose group and placebo group, respectively. The median time to fever clearance was 18.29 h, 20.08 h and 25.00 h (P = 0.0018), respectively, and the proportion of afebrile patients was 92.4%, 89.7% and 71.4% (P = 0.0002), respectively. There was a significant difference in the disappearance time and disappearance rate of all symptoms and of individual symptoms. No serious adverse events were found. Conclusions: Binafuxi granules can dose-dependently shorten the fever course and improve clinical symptoms in patients suffering from the common cold with fever.

Phenolic Compounds from Arundina graminifolia and Their Anti-Tobacco Mosaic Virus Activity

Gao, Xuemei,Yang, Liying,Shen, Yanqiong,Shu, Lidang,Li, Xuemei,Hu, Qiu-Fen Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

Safety and antifatigue effect of Korean Red Ginseng capsule: A randomized, double-blind and placebo-controlled clinical trial

Yi Yang,Hong Wang,Ming Zhang,Mengxue Shi,Cailing Yang,Qiang Ni,Qi Wang,Jing Li,Xuemei Wang,Chen Zhang,Zhi Li 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.4

Background: In physical activity or labor, the human body is in a state of high intensity stress, and allparts or physiological functions of the body respond positively to maintain or balance the need formovement. The human body has many physiological changes in the process of movement, and fatigue isthe external manifestation of various complex changes inside the human body. Fatigue is also a physiologicalmechanism of self-protection after the body reaches a certain level of activity, which can preventthe occurrence of life-threatening excessive functional failure. The generation of fatigue is a very complexprocess, and its mechanism has not been concluded yet. Therefore, it is an important work to search andscreen the effective components of natural plants that have anti-fatigue effect and to explore theirmechanism. Methods: This was a 8-week, randomized, double-blind, placebo-controlled clinical trial. A total of 110subjects who passed physical examination were included according to the scheme design, and randomlydivided into a test group which was given KRG and a placebo control group. The calculation is carried outaccording to the standard of sub-high-intensity exercise test. Results: There was no adverse effect on safety index of subjects after taking red ginseng capsule. AfterKRG treatment, subjective strength grade is significant lower than placebo treatment. Blood lactic acidcontent is significantly get lower after trial in KRG group, and significant lower than placebo group. Creatine phosphokinase(CK) content is significantly get lower after trial in KRG group, and significantlower than placebo group. Conclusion: According to the criterion in the test scheme, the result shows that KRG is helpful onrelieving physical fatigue.

Effects of Gal-13 on the Content of Immunoglobulin, Proliferation of Lymphocyte and Antibody Titers after Vaccination with Infectious Bursal Disease Virus Vaccine in Chickens

Yang, Yurong,Jiang, Yibao,She, Ruiping,Peng, Kaisong,Zhou, Xuemei,Yin, Qingqiang,Wang, Decheng,Liu, Tianlong,Bao, Huihui Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.3

Gal-13 is an antimicrobial peptide isolated from chicken intestine. Ninety chickens were randomly divided into two groups (45 chickens for each group) to determine the effect of oral administration of Gal-13 on the acquired immune response. The chickens in the first group were fed a diet without Gal-13 as the control, and the chickens in the second group were fed the same diet, except that Gal-13 ($1{\mu}g/ml$) was suspended in drinking water just after hatching. Samples of blood, thymus, bursa of fabricius and spleen were taken at day 1, 4, 7, 10 and 17. The chickens in both groups received infectious bursal disease virus vaccine at day 20, and then sera samples were collected for analysis at 14, 21, 28 and 35 days after vaccination. The results showed: (1) Gal-13 could enhance the content of immunoglobulin (Ig)G at the age of 4 to10 days (p<0.05) and IgM at the age of 4 and 10 days (p<0.05) in the serum; (2) In vitro experiments showed that Gal-13 (0.625-1.250${\mu}g/ml$) enhanced the proliferation of peripheral blood lymphocytes of the chickens stimulated by lipopolysaccharide (LPS) and concanavlin A (ConA). Compared to the control, Gal-13 (1 ${\mu}g/ml$) enhanced the proliferation of bursa lymphocytes at 17 days of age (p<0.01) and thymus lymphocytes at 7 days of age (p<0.01), but restrained lymphocyte proliferation in chicken spleen and differed significantly at day 10 (p<0.01); (3) Gal-13 enhanced infectious bursal disease virus antibody in sera of chickens 21 days after infectious bursal disease virus vaccine administration (p<0.05). These results suggested that Gal-13 could modulate adaptive immune responses of chickens.

SYNTHESIZING A WELL-ALIGNED CARBON NANOTUBE FOREST WITH HIGH QUALITY VIA THE NEBULIZED SPRAY PYROLYSIS METHOD BY OPTIMIZING ULTRASONIC FREQUENCY

ZHI YANG,HUAGUI NIE,XUEMEI ZHOU,ZHEN YAO,SHAOMING HUANG,XIAOHUA CHEN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.4

Nebulized spray pyrolysis (NSP) offers ease of scaling into an industrial scale process method for large-scale CNT synthesis. In the method, ultrasonic frequency plays an important role in the formation of well-aligned carbon nanotubes (CNTs). In this work, we obtain well-aligned CNTs with high quality by optimizing ultrasonic frequency via the NSP method. SEM, TEM and HRTEM results show that the aligned CNTs obtained under an ultrasonic frequency of 1.8 MHz have good alignment, uniform nanotube length (~200 μm), high density and no trace of other carbon nanoparticles. The Raman spectra result further confirms that the aligned CNTs have a high graphitization degree. Furthermore, we find that ultrasonic frequency is an important factor in determining the nanotube diameter. The higher the ultrasonic frequency, the smaller the nanotube diameter. This rule may be very helpful in the preparation of large-scale well-aligned CNTs with high quality and diameter-controlled at low cost.

Characterization and development of EST-SSR markers to study the genetic diversity and populations analysis of Jerusalem artichoke (Helianthus tuberosus L.)

Shipeng Yang,Qiwen Zhong,Jie Tian,Lihui Wang,Mengliang Zhao,Li Li,Xuemei Sun 한국유전학회 2018 Genes & Genomics Vol.40 No.10

In recent years, Jerusalem artichoke has received widespread attention as a novel source of sugar, biofuel, and animal feed. Currently, only few gDNA-SSRs derived from sunflower were verified in the Jerusalem artichoke; therefore, it is particularly important to develop SSR primer markers that belonged to Jerusalem artichoke resources. Using EST data to develop EST-SSR markers is simple and effective. In order to understand the general characteristics of SSR markers in Jerusalem artichoke EST sequences and accelerate the use of SSR markers in Jerusalem artichoke research. This study used 40,370 sequenced unigene fragments and MISA software to identify SSR loci. The 48 pairs of EST-SSR primers assessed for the identification of 45 varieties of Jerusalem artichoke. Cluster, genetic diversity parameters and AMOVA analysis was conducted using the genetic similarity coefficient, revealing genetic differences between 48 genetic material. A total of 1204 SSR loci were identified with 13 different types of repeats, distributed among 1020 EST sequences, of which trinucleotide repeats were the most common, accounting for 38.21% of the total SSR loci. Among the 44 repeat motifs, AG/CT, AAG/ CTT, and ATC/ATG motifs had the highest frequencies, accounting for 22.45, 14.71, and 7.84% of all motifs, respectively. From these sequences, 48 pairs of EST-SSR primers were designed, and 22 primer pairs for loci with high polymorphism were selected to analyze the genetic diversity of 45 Jerusalem artichoke germplasm sources. The results indicated that the variation range of the effective number of alleles for 22 primers ranged between 1.7502 and 4.5660. The Shannon’s information index ranged between 0.6200 and 1.6423. The variation range of PIC ranged between 0.3121 and 0.6662 with an average of 0.5184. Cluster analysis was conducted using the genetic similarity coefficient, revealing significant genetic differences between Asian and European genetic material. Cluster analysis revealed a relationship between the genotypes and geographic origins of the Jerusalem artichoke. The results of AMOVA as well as the genetic identity and genetic distance in the Jerusalem artichoke population showed that there presented certain genetic heterogeneity in Jerusalem artichoke genetic structure of 45 samples from seven different geographic populations. The Jerusalem artichoke EST-SSR marker system established in this study provides an effective molecular marker system for future research focused on Jerusalem artichoke genetic diversity and the breeding of new varieties.

INVESTIGATION OF HOMOLOGOUS SERIES AS PRECURSORY HYDROCARBONS FOR ALIGNED CARBON NANOTUBE FORMATION BY THE SPRAY PYROLYSIS METHOD

ZHI YANG,HUAGUI NIE,XUEMEI ZHOU,ZHEN YAO,SHAOMING HUANG,XIAOHUA CHEN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.3

The precursory carbon source is one of the key parameters which govern the formation of carbon nanotubes (CNTs). In this work, by selecting four homologous series, namely n-pentane, n-hexane, n-heptane and n-octane, as investigated targets, we comparatively study the relationship between thermodynamic properties of the precursory carbon source and formation of aligned CNTs. We find that all of these alkanes are favored for the growth of aligned CNTs in a suitable growth environment. But only n-heptane can yield the aligned CNTs with relatively high quality, high yield and narrow diameter distribution. Furthermore, after considering the link between thermodynamic properties of the precursory carbon source and the morphology characteristic of the nanotube samples, we find that the Gibbs free energy and formation enthalpy of precursory carbon sources play critical roles in the nanotube formation. In additions some possible explanations are proposed to better understand these phenomena. These rules will be very helpful in making clearer the formation mechanism from the precursory carbon sources to the CNTs, and preparing large-scale aligned CNTs with diameter control at low cost.

Analysis of the Bacterial Community in a Full-scale Printing and Dyeing Wastewater Treatment System Based on T-RFLP and 454 Pyrosequencing

Qingxiang Yang,Jia Wang,Xinkuan Han,Yuanyuan Xu,Dong Liu,Hongxin Hao,Xuemei L,Yuhui Guo,Tianqi Niu,Shiyue Qi 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

In this study, the bacterial dynamics andstructure compositions in the two-stage biological processof a full-scale printing and dyeing wastewater (PDW)treatment system were traced and analyzed by terminalrestriction fragment length polymorphism (T-RFLP) and454 pyrosequencing techniques. T-RFLP analysis showedthat the microbial communities experienced significantvariation in the process of seed sludge adaptation to thePDW environments and were in constant evolution duringthe whole running period of the system, despite theconstant COD and color removal effects. Pyrosequencingresults indicated that the two-stage biological systemharbored rather diverse bacteria, with Proteobacteria beingthe predominant phylum during the steady running period,although its microbial compositions differed. The firststageaerobic tank was dominated by α-Proteobacteria(89.05% of Proteobacteria), whereas in the second-stageaerobic tank, β- and γ-Proteobacteria, besides α-Proteobacteria,were the dominant bacterial populations.

Angle-sensitive Pixels Based on Subwavelength Compound Gratings

Yunlong Meng,Xuemei Hu,Cheng Yang,Xinyu Shen,Xueyun Cao,Lankun Lin,Feng Yan,Tao Yue 한국광학회 2022 Current Optics and Photonics Vol.6 No.4

In this paper, we present a new design for angle-sensitive pixels (ASPs). The proposed ASPs take advantage of subwavelength compound gratings to capture the light angle, which enables pixel size to reach the wavelength scale of 0.7 μm × 0.7 μm. The subwavelength compound gratings are implement-ed by the wires of the readout circuit inherent to the standard complementary metal–oxide–semiconductor manufacturing process, thus avoiding additional off-chip optics or post-processing. This technique allows the use of two pixels for horizontal or vertical angle detection, and can determine the light’s angle in the range from −45° to +45°. The proposed sensor enables surface-profile reconstruction of mi-croscale samples using a lensless imaging system.