Biocatalysis and Fermentation Technology : Thermostable Sites and Catalytic Characterization of Xylanase XYNB of Aspergillus niger SCTCC 400264

( Xin Ran Li ),( Hui Xu ),( Jie Xie ),( Qiao Fu Yi ),( Wei Li ),( Dai Rong Qiao ),( Yi Cao ),( Yu Cao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4

In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times that of E. coli, and the Vmax and specific activity of XynB reached 2,547.7 umol/mg and 4,757 U/mg, respectively. XynB still had 74% residual enzyme activity after 30 min of heat treatment at 80°C. From the van der Waals force analysis of XYNB (ACN89393 and AAS67299), there is one more oxygen radical in AAS67299 in their catalytic site, indicating that the local cavity is much more free, and it is more optimal for substrate binding, affinity reaction, and proton transfer, etc, and e ventually i ncreasing enzyme activity. The H-bonds analysis of XYNB indicated that there are two more H-bonds in the 33rd Ser of XYNB (AAS67299) than in the 33rd Ala(ACN89393 ), and two H-bonds between Ser70 and Asp67.

기획주제(企劃主題) : 동아시아 한자(漢字),한자교육(漢字敎育)의 현황(現況)과 과제(課題); ?圈識字法與天津市低年級識字?學

( Xin Ying Fu ),( Jing Yi Liu ) 한국한자한문교육학회 2013 漢字 漢文敎育 Vol.30 No.-

지연반복한자교육법(?圈識字)은 천진의 초등학교 저학년의 한자교육에서 매우 중요한 지위를 점하고 있으며, 그 효과 또한 실제적으로 증명된다. 천진에서의 이런 경험은 다른 한자교육법과 비교해 볼 때, 지연반복한자교육법은 아동의 기억현상과 부합되며, 그 효과 또한 매우 높게 나타난다. 저학년 한자교육은 지연반복한자교육법이 핵심이 되며, 다른 기타 한자교육법은 부가 되어, 이러한 교육 경험을 확대시킬 필요가 있다. 이는 집중한자교육의 이념을 구현한 것이나 그 스스로도 독특한 특징을 가지고 있다. “Delayed repetition literacy” stands an important position on literacy teaching in lower grade in Tianjin, and the effects is obvious. Relative to others, this literacy teaching accords with children`s memory rule, and promotes the teaching effects. It embody the ideal of the “focus literacy”, but also has its own characters and so, deserves popularizing.

A Study on the Scenario Transparency of Civil Intelligent Adjudication in China

주복용 ( Fu-yong Zhu ),퉁신위 ( Xin-yu Tong ),가오이헝 ( Yi-heng Gao ) 한중사회과학학회 2024 한중사회과학연구 Vol.22 No.1

Technology and civil adjudication of two-way run to write a new chapter of civil intelligent adjudication, intelligent technology for the adjudication of empowerment, help to standardize the adjudication of the operation of the program, enhance the quality of the adjudication of the effectiveness of the adjudication of the realization of the goals of the adjudication of great significance. However, in practice, due to the lack of transparency of pre-trial meetings, court hearings, adjudication and deviation and other scenarios, in particular, the algorithms involved are mostly protected by technology companies as trade secrets. The black box of algorithms makes it difficult for the parties to civil cases and the public to know the actual operation of the algorithms and the logic behind the adjudication actions, and it is impossible to dispel the reasonable doubts of the parties and the public about the adjudication results, which may exacerbate the crisis of the acceptability of civil intelligent adjudication. To this end, we take scene transparency as the perspective, typify the scenes of civil intelligent adjudication, construct intelligent transparency models from different scenes such as pre-trial preparation, trial, adjudication and adjudication deviation, promote the deep integration between technology and adjudication activities, promote the connection, switching and integration of material space and information space through technology, shape the theoretical models of transparency in different scenes, and improve the compatibility between the theoretical models of scene transparency and the development of intelligent adjudication technology. It also improves the adaptability between the theoretical model of scene transparency and the development of intelligent adjudication technology. Based on the justice goal of scene transparency research and development, and in accordance with the principle of human-machine symbiosis, the civil adjudication mode of human-machine symbiosis is constructed to promote the traditional theory of “field justice” to the theory of “digital justice”, improve the digital system of civil case resolution, and promote the realization of the fusion of technology and law. It also promotes the digitalization system of civil case resolution, promotes the integration of technology and law, and promotes the integration of emotion, reason and law in the adjudication results while enhancing the transparency of the intelligent adjudication scenario, so as to continuously improve the acceptability of the civil adjudication results, and to build up an ecological system of trust for civil intelligent adjudication.

Study on ANT-based Wireless Network for Wearable Medical Monitoring

Niu Fu Rong,Yan Yi Xin 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.3

Such technical features of ANT as protocol stack structure, data frame format and channel configuration are first introduced in the paper, and then targeted at the groups that need real-time medical monitoring, an ANT protocol-based wireless network medical monitoring system is established to realize real-time acquisition of corresponding physiological data. This system adopts the chip NRF24AP2 compatible with ANT protocol as the core component of wireless network nodes of the medical monitoring system to conduct design of software and hardware. It is shown through power consumption analysis and communication reliability testing of the wireless network that this network features low power consumption under the premise of operating reliability, applicable to various applications giving priority to low power consumption.

Risk Factors for Anxiety in Major Depressive Disorder Patients

Li-Min Xin,Lin Chen,Zhen-Peng Ji,Suo-Yuan Zhang,Jun Wang,Yan-Hong Liu,Da-Fang Chen,Fu-De Yang,Gang Wang,Yi-Ru Fang,Zheng Lu,Hai-Chen Yang,Jian Hu,Zhi-Yu Chen,Yi Huang,Jing Sun,Xiao-Ping Wang,Hui-Chun 대한정신약물학회 2015 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.13 No.3

Objective: To analyze the sociodemographic and clinical factors related to anxiety in patients with major depressive disorder (MDD). Methods: This study involved a secondary analysis of data obtained from the Diagnostic Assessment Service for People with Bipolar Disorders in China (DASP), which was initiated by the Chinese Society of Psychiatry (CSP) and conducted from September 1, 2010 to February 28, 2011. Based on the presence or absence of anxiety-related characteristics, 1,178 MDD patients were classified as suffering from anxious depression (n=915) or non-anxious depression (n=263), respectively. Results: Compared with the non-anxious group, the anxious-depression group had an older age at onset (t=−4.39, p<0.001), were older (t=−4.69, p<0.001), reported more lifetime depressive episodes (z=−3.24, p=0.001), were more likely to experience seasonal depressive episodes (χ2=6.896, p=0.009) and depressive episodes following stressful life events (χ2=59.350, p <0.001), and were more likely to have a family history of psychiatric disorders (χ2=6.091, p=0.014). Their positive and total scores on the Mood Disorder Questionnaire (MDQ) and the 32-item Hypomania Checklist (HCL-32) (p<0.05) were also lower. The logistic regression analysis indicated that age (odds ratio [OR]=1.03, p<0.001), a lower total MDQ score (OR=0.94, p=0.011), depressive episodes following stressful life events (OR=3.04, p<0.001), and seasonal depressive episodes (OR=1.75, p=0.039) were significantly associated with anxious depression. Conclusion: These findings indicate that older age, fewer subclinical bipolar features, an increased number of depressive episodes following stressful life events, and seasonal depressive episodes may be risk factors for anxiety-related characteristics in patients with MDD.

Study on Photosynthesis Reaction of Plants at Different Light Wavelengths Mix Ratio under Low Power

( Chun-yu Tsai ),( Xin-yi Lin ),( Yung-fu Yen ),( Chyung Ay ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

The environmental factors such as light, carbon dioxide, temperature, and nutrition are very important and closely related to the stable control of plant growth in plant factories. However, how to configure the light source at different light wavelengths to increase the physiological response of chlorophyll is also a knowledge. In this study, Boston Lettuce and Red Lettuce (Ziyan) were used as samples and planted in the NFT (Nutrient Film Technology) cultivation system. The effects of different light wavelengths combination on photosynthesis were analyzed to find the optimal ratio of light sources. In addition, sample analysis was performed on these two plants to verify the correctness of the light wavelengths combination ratio. The preliminary results show that the green leaves Boston lettuce have the highest photosynthetic efficiency under the combination of the red wavelength (38.4 μmol) + blue wavelength (57.6 μmol) + green wavelength (24 μmol), while the red leaves Ziyan lettuce had the best photosynthetic efficiency under red wavelength (43.2 μmol) + blue wavelength (64.8 μmol) + yellow wavelength (12 μmol). At the end of the experiment, plant sample analysis will be performed using these two ratios of light wavelengths, verifying that the leaves of two different lettuces strains have its most suitable ratio of light wavelengths, allowing plants to grow efficiently, improve yield, quality and other advantages.

Structure-based design, structure–activity relationship analysis, and antitumor activity of diaryl ether derivatives

Shao-Mei Yang,Fu-Nan Li,Zhi-Ning Huang,Zhong-Shi Zhou,Jin Hou,Man-Yi Zheng,Li-Juan Wang,Yu Jiang,Xin-Yi Zhou,Qiu-Yue Chen,Shan-Hua Li 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

To identify novel therapeutic agents to treatcancer, we synthesized a series of diaryl ether derivatives. Structure–activity relationship studies revealed that thepresence of a chlorine or hydroxyl at the para-position onthe phenyl ring (5h or 5k) significantly enhanced antitumoractivity. Compound 5h had stronger growth inhibitory activityin HepG2, A549, and HT-29 cells than compound 5k,with IC50 values of 2.57, 5.48, and 30.04 lM, respectively. Compound 5h also inhibited the growth of other cells lines,including Hep3B, PLC/PRF5, SMMC-7721, HeLa, andA375, with IC50 values of 2.76, 4.26, 29.66, 18.86, and10.21 lM, respectively. The antitumor activity of compound5h was confirmed by a colony forming assay. Further,our results indicated that the antitumor activity ofcompound 5h may be mediated by enhancing expression ofp21 and cl-caspase3, and leading to apoptosis of cancercells.

Expression of IER3 in Primary Hepatocarcinoma: Correlation with Clinicopathological Parameters

Liu, Zhong,Wang, Xin-Mei,Jia, Tong-Fu,Zhai, Yi,Sun, Ling-Yan,Cheng, Yu-Ping,Zhang, Yue-Min,Liu, Shi-Hai,Liang, Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Studies indicate the immediate early response gene 3 (IER3) is involved in many biological processes. Recently, it was discovered that IER3 plays an important role in tumorigenesis and tumor progression. Thus it may be a valuable biomarker in tumor. This study was designed to investigate the expression status of IER3 in primary hepatocarcinoma (PHC) and correlation with clinicopathological parameters. Materials and Methods: Real-time PCR was performed to evaluate the expression levels of IER3 in 62 pathologically diagnosed human PHC specimens. Results: A statistically significant association was disclosed between the expression of IER3 and P53 mutant protein (short for P53), Ki-67, EGFR and the biggest diameter, differentiation grade of tumor. Conclusions: This work is the first to shed light on the potential clinical usefulness of IER3, as an efficient tumor biomarker in PHC.

Neutrophil-inspired photothermo-responsive drug delivery system for targeted treatment of bacterial infection and endotoxins neutralization

Chengnan Li,Yingying Gan,Zongshao Li,Mengjing Fu,Yuzhen Li,Xinran Peng,Yongqiang Yang,Guo‑bao Tian,Yi Yan Yang,Peiyan Yuan,Xin Ding 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background P. aeruginosa, a highly virulent Gram-negative bacterium, can cause severe nosocomial infections, and it has developed resistance against most antibiotics. New therapeutic strategies are urgently needed to treat such bacterial infection and reduce its toxicity caused by endotoxin (lipopolysaccharide, LPS). Neutrophils have been proven to be able to target inflammation site and neutrophil membrane receptors such as Toll-like receptor-4 (TLR4) and CD14, and exhibit specific affinity to LPS. However, antibacterial delivery system based on the unique properties of neutrophils has not been reported. Methods A neutrophil-inspired antibacterial delivery system for targeted photothermal treatment, stimuli-responsive antibiotic release and endotoxin neutralization is reported in this study. Specifically, the photothermal reagent indocyanine green (ICG) and antibiotic rifampicin (RIF) are co-loaded into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NP-ICG/RIF), followed by coating with neutrophil membrane to obtain antibacterial delivery system (NM-NP-ICG/RIF). The inflammation targeting properties, synergistic antibacterial activity of photothermal therapy and antibiotic treatment, and endotoxin neutralization have been studied in vitro. A P. aeruginosa-induced murine skin abscess infection model has been used to evaluate the therapeutic efficacy of the NM-NP-ICG/RIF. Results Once irradiated by near-infrared lasers, the heat generated by NP-ICG/RIF triggers the release of RIF and ICG, resulting in a synergistic chemo-photothermal antibacterial effect against P. aeruginosa (~ 99.99% killing efficiency in 5 min). After coating with neutrophil-like cell membrane vesicles (NMVs), the nanoparticles (NM-NP-ICG/RIF) specifically bind to inflammatory vascular endothelial cells in infectious site, endowing the nanoparticles with an infection microenvironment targeting function to enhance retention time. Importantly, it is discovered for the first time that NMVs-coated nanoparticles are able to neutralize endotoxins. The P. aeruginosa murine skin abscess infection model further demonstrates the in vivo therapeutic efficacy of NM-NP-ICG/RIF. Conclusion The neutrophil-inspired antibacterial delivery system (NM-NP-ICG/RIF) is capable of targeting infection microenvironment, neutralizing endotoxin, and eradicating bacteria through a synergistic effect of photothermal therapy and antibiotic treatment. This drug delivery system made from FDA-approved compounds provides a promising approach to fighting against hard-to-treat bacterial infections.

A novel M2e-multiple antigenic peptide providing heterologous protection in mice

Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Xiu-Hui Wang,Guo-Xin Li,Yi-Feng Jiang,Wu Tong,Guangzhi Tong 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.