miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro

Hu, Xiaoyi,Miao, Jiyu,Zhang, Min,Wang, Xiaofei,Wang, Zhenzhen,Han, Jia,Tong, Dongdong,Huang, Chen Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.5

Studies have revealed that miR-103a-3p contributes to tumor growth in several human cancers, and high miR-103a-3p expression is associated with poor prognosis in advanced gastric cancer (GC) patients. Moreover, bioinformatics analysis has shown that miR-103a-3p is upregulated in The Cancer Genome Atlas (TCGA) stomach cancer cohort. These results suggest that miR-103a-3p may function as an oncogene in GC. The present study aimed to investigate the role of miR-103a-3p in human GC. miR-103a-3p expression levels were increased in 33 clinical GC specimens compared with adjacent nontumor stomach tissues. Gain- and loss-of-function studies were performed to identify the correlation between miR-103a-3p and tumorigenesis in human GC. Inhibiting miR-103a-3p suppressed GC cell proliferation and blocked the S-G2/M transition in MKN-45/SGC-7901 cells, whereas miR-103a-3p overexpression improved GC cell proliferation and promoted the S-G2/M transition in vitro. Bioinformatics and dual-luciferase reporter assays confirmed that ATF7 is a direct target of miR-103a-3p. Analysis of the TCGA stomach cancer cohort further revealed that miR-103a-3p expression was inversely correlated with ATF7 expression. Notably, silencing ATF7 showed similar cellular and molecular effects as miR-103a-3p overexpression, namely, increased GC cell proliferation, improved CDK2 expression and decreased P27 expression. ATF7 overexpression eliminated the effects of miR-103a-3p expression. These findings indicate that miR-103a-3p promotes the proliferation of GC cell by targeting and suppressing ATF7 in vitro.

miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro

Xiaoyi Hu,Jiyu Miao,Min Zhang,Xiaofei Wang,Zhenzhen Wang,Jia Han,Dongdong Tong,Chen Huang 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.5

Studies have revealed that miR-103a-3p contributes to tumor growth in several human cancers, and high miR-103a-3p expression is associated with poor prognosis in advanced gastric cancer (GC) patients. Moreover, bioinformatics analysis has shown that miR-103a-3p is upregulated in The Cancer Genome Atlas (TCGA) stomach cancer cohort. These results suggest that miR-103a-3p may function as an oncogene in GC. The present study aimed to investigate the role of miR-103a-3p in human GC. miR-103a-3p expression levels were increased in 33 clinical GC specimens compared with adjacent nontumor stomach tissues. Gain- and loss-of-function studies were performed to identify the correlation between miR-103a-3p and tumorigenesis in human GC. Inhibiting miR-103a-3p suppressed GC cell proliferation and blocked the S-G2/M transition in MKN-45/SGC-7901 cells, whereas miR-103a-3p overexpression improved GC cell proliferation and promoted the S-G2/M transition in vitro. Bioin-formatics and dual-luciferase reporter assays confirmed that ATF7 is a direct target of miR-103a-3p. Analysis of the TCGA stomach cancer cohort further revealed that miR-103a-3p expression was inversely correlated with ATF7 expression. Notably, silencing ATF7 showed similar cellular and molecular effects as miR-103a-3p overexpression, namely, increased GC cell proliferation, improved CDK2 expression and decreased P27 expression. ATF7 overexpression eliminated the effects of miR-103a-3p expression. These findings indicate that miR-103a-3p promotes the proliferation of GC cell by targeting and suppressing ATF7 in vitro.

MicroRNA-455-3p promotes TGF-β signaling and inhibits osteoarthritis development by directly targeting PAK2

Shu Hu,Xiaoyi Zhao,Guping Mao,Ziji Zhang,Xingzhao Wen,Chengyun Zhang,Weiming Liao,Zhiqi Zhang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

MicroRNAs (miRNAs, miR) play a key role in the pathogenesis of osteoarthritis (OA). Few studies have examined theregulatory role of P21-activated kinases (PAKs), a family of serine/threonine kinases, in OA. The aim of this study was todetermine whether miR-455-3p can regulate cartilage degeneration in OA by targeting PAK2. MiR-455-3p knockoutmice showed significant degeneration of the knee cartilage. MiR-455-3p expression increased and PAK2 expressiondecreased in the late stage of human adipose-derived stem cell (hADSC) chondrogenesis and in chondrocytesaffected by OA. Furthermore, in both miR-455-3p-overexpressing chondrocytes and PAK2-suppressing chondrocytes,cartilage-specific genes were upregulated, and hypertrophy-related genes were downregulated. A luciferase reporterassay confirmed that miR-455-3p regulates PAK2 expression by directly targeting the 3′-untranslated regions (3′UTRs)of PAK2 mRNA. IPA-3, a PAK inhibitor, inhibited cartilage degeneration due to OA. Moreover, suppressing PAK2promoted R-Smad activation in the TGF/Smad signaling pathway in chondrocytes. Altogether, our results suggest thatmiR-455-3p promotes TGF-β/Smad signaling in chondrocytes and inhibits cartilage degeneration by directlysuppressing PAK2. These results thus indicate that miR-455-3p and PAK2 are novel potential therapeutic agents andtargets, respectively, for the treatment of OA.

Mobile Manufacturer or Service provider? An Empirical Study on Consumers’ Adoption Intention

Feng Hu,Xiaoyi Du,Yong Liu 보안공학연구지원센터(IJUNESST) 2010 International Journal of u- and e- Service, Scienc Vol.3 No.2

Correlation of UGT2B7 Polymorphism with Cardiotoxicity in Breast Cancer Patients Undergoing Epirubicin/Cyclophosphamide-Docetaxel Adjuvant Chemotherapy

Hai Li,Bo Hu,Zhe Guo,Xueqing Jiang,Xinyu Su,Xiaoyi Zhang 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.1

Purpose: The present study aimed to investigate correlations between uridine glucuronosyltransferase 2B7 (UGT2B7) -161 singlenucleotide polymorphism C to T (C>T) and the occurrence of cardiotoxicity in Chinese breast cancer (BC) patients undergoingepirubicin/cyclophosphamide-docetaxel (EC-D) adjuvant chemotherapy. Materials and Methods: 427 BC patients who had underwent surgery were consecutively enrolled in this prospective cohortstudy. All patients were scheduled to receive EC-D adjuvant chemotherapy regimen, and they were divided into UGT2B7 -161 CC(n=141), UGT2B7 -161 CT (n=196), and UGT2B7 -161 TT (n=90) groups according to their genotypes. Polymerase chain reactionwas performed for determination of UGT2B7 -161 genotypes. Cardiotoxicity was defined as an absolute decline in left ventricularejection fraction (LVEF) of at least 10% points from baseline to a value less than 53%, heart failure, acute coronary artery syndrome,or fatal arrhythmia. Results: LVEF values were lower at cycle (C) 4, C8, 3 months after chemotherapy (M3), M6, M9, and M12 compared to C0 (allp<0.001), in BC patients undergoing EC-D adjuvant chemotherapy. Cardiotoxicity was recorded for 4.2% of the overall populationand was lowest in the UGT2B7 -161 TT group (1.1%), compared to UGT2B7 -161 CT (3.1%) and UGT2B7 -161 CC (7.8%) group(p=0.026). Multivariate logistic regression revealed that UGT2B7 -161 T allele could independently predict a low occurrence ofcardiotoxicity in BC patients undergoing EC-D adjuvant chemotherapy (p=0.004). Conclusion: A UGT2B7 -161 T allele serves as a potential biomarker for predicting a low occurrence of cardiotoxicity in BC patientsundergoing EC-D adjuvant chemotherapy.

Exploring the Categories and Models of Everything as a Service (XaaS)

Yucong Duan,Wenlong Feng,Xiaoyi Zhou,Linfeng Dong,Tao Hu,Honghao Gao 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.6

Ultrafast dynamics control on ablation of Cu using shaped femtosecond pulse trains

Deng Jiannan,Qi Hongxia,Liu Xinyi,Li Xiaoyi,Tong Qiunan,Lian Zhenzhong,Li Juan,Bo Jinqiu,Fei Dehou,Chen Zhou,Hu Zhan 한국물리학회 2021 Current Applied Physics Vol.26 No.-

The ablation processes of Cu film are investigated using temporal shaped femtosecond pulse trains. The depth is modulated by changing the number and interval of the sub-pulses. The underlying ultrafast dynamic processes are discussed based on plasma shielding and electron multiple heating mechanisms. When the sub-pulse interval is less than 0.4 ps electron multiple heating is the dominant mechanism, while the plasma shielding dominates the subsequent ablation processes when the sub-pulse interval is larger than 0.4 ps. The curve of depth obtained by three pulse trains shows more significant oscillation as the function of sub-pulse interval under the lowfluence. We propose that the oscillation of depth is due to the coherent phonon oscillation excited by the pulse train. The study provides a basis for giving insight into the ultrafast dynamics for improving micromachining and nano-fabrications using shaped femtosecond pulse trains.

Promotional effect of Ce in NH3-SCO and NH3-SCR reactions over Cu-Ce/ SCR catalysts

Wenjie Liu,Yifei Long,Shinian Liu,Yongyan Zhou,XinTong,Yajie Yin,Xiaoyi Li,Kang Hu,Jiangjun Hu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

Commercial V-W-Ti catalysts were modified with Cu and different contents of Ce to remove slip ammoniaand remaining nitrogen oxide from the end stage of SCR technology. The activities of NH3 oxidation byO2 (NH3-SCO) and NO reduction by NH3 (NH3-SCR) of these catalysts were examined from 150 to 400 ℃. The consequences indicated that the addition of Ce and Cu could considerably enhance NH3 oxidation andNO reduction capacity of the catalysts and Ce was conducive to reduce the detrimental effects of H2O andSO2. The characterization tests indicated that the surface active oxygen increased because of the interactionbetween V, Cu and Ce. Moreover, the loaded Ce and Cu contributed to enhance the surface acidityand redox property of SCR catalysts.