A positive feedback loop of heparanase/ syndecan1/nerve growth factor regulates cancer pain progression

Xiaohu Su,Bingwu Wang,Zhaoyun Zhou,Zixian Li,Song Tong,Simin Chen,Nan Zhang,Su Liu,Maoyin Zhang 대한통증학회 2023 The Korean Journal of Pain Vol.36 No.1

Background: The purpose of this research was to assess the role of heparanase (HPSE)/syndecan1 (SDC1)/nerve growth factor (NGF) on cancer pain from melanoma. Methods: The influence of HPSE on the biological function of melanoma cells and cancer pain in a mouse model was evaluated. Immunohistochemical staining was used to analyze HPSE and SDC1. HPSE, NGF, and SDC1 were detected using western blot. Inflammatory factors were detected using ELISA assay. Results: HPSE promoted melanoma cell viability, proliferation, migration, invasion, and tumor growth, as well as cancer pain, while SST0001 treatment reversed the promoting effect of HPSE. HPSE up-regulated NGF, and NGF feedback promoted HPSE. High expression of NGF reversed the inhibitory effect of HPSE down-regulation on melanoma cell phenotype deterioration, including cell viability, proliferation, migration, and invasion. SST0001 down-regulated SDC1 expression. SDC1 reversed the inhibitory effect of SST0001 on cancer pain. Conclusions: The results showed that HPSE promoted melanoma development and cancer pain by interacting with NGF/SDC1. It provides new insights to better understand the role of HPSE in melanoma and also provides a new direction for cancer pain treatment.

Numerical investigation on seismic performance of reinforced rib-double steel plate concrete combination shear wall

Zhou Longyun,Li Xiaohu,Li Xiaojun 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1

Double steel plate concrete composite shear wall (SCSW) has been widely utilized in nuclear power plants and high-rise structures, and its shear connectors have a substantial impact on the seismic performance of SCSW. Therefore, in this study, the mechanical properties of SCSW with angle stiffening ribs as shear connections were parametrically examined for the reactor containment structure of nuclear power plants. The axial compression ratio of the SCSW, the spacing of the angle stiffening rib arrangement and the thickness of the angle stiffening rib steel plate were selected as the study parameters. Four finite element models were constructed by using the finite element program named ABAQUS to verify the experimental results of our team, and 13 finite element models were established to investigate the selected three parameters. Thus, the shear capacity, deformation capacity, ductility and energy dissipation capacity of SCSW were determined. The research results show that: compared with studs, using stiffened ribs as shear connectors can significantly enhance the mechanical properties of SCSW; When the axial compression ratio is 0.3–0.4, the seismic performance of SCSW can be maximized; with the lowering of stiffener gap, the shear bearing capacity is greatly enhanced, and when the gap is lowered to a specific distance, the shear bearing capacity has no major affect; in addition, increasing the thickness of stiffeners can significantly increase the shear capacity, ductility and energy dissipation capacity of SCSW. With the rise in the thickness of angle stiffening ribs, the improvement rate of each mechanical property index slows down. Finally, the shear bearing capacity calculation formula of SCSW with angle stiffening ribs as shear connectors is derived. The average error between the theoretical calculation formula and the finite element calculation results is 8% demonstrating that the theoretical formula is reliable. This study can provide reference for the design of SCSW.

Hole Diameter Deviation Control Method for Robotic Hole-Making System Based on Presser Foot Action

Xiaohu Zheng,Guanyao Zhang,Chao Zhou,Guoqiang Guo 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.2

Robotic hole-making systems are widely used in aerospace, automotive and other fields, mainly for diverse and structurally complex parts processing tasks. However, the industrial robot automatic hole-making system is an open-chain multi-bar tandem mechanism, which is prone to quality problems such as unstable cutting process, large hole diameter deviation and hole wall surface defects when performing tasks such as hole-making. This article constructs a stiffness enhancement model for the axial and horizontal directions of the robot presser foot based on the robot end stiffness enhancement method and analyzes the impact of pressure changes on hole-making quality under the action of the presser foot. Through experimental data analysis, the aperture deviation decreased by 32.91% compared to before the pressure foot and the average height of outlet burrs decreased by 85.3%, verifying the feasibility of the method.

Synthesis of natural glucomannan derivative as a highly-efficient green inhibitor for mild steel in the simulated seawater

Xiaohu Luo,Bo Chen,Ji Li,Bang Lan,Chenliang Zhou,Zhengxing Ren,Chenggang Ci,Yali Liu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.124 No.-

Herein, we report a rational design and synthesis of a highly-efficient and eco-friendly glucomannanderivative (GA-2) by the introduction of the thiazole and Schiff base groups into glucomannan (GA). After confirmation of GA-2 by the spectroscopic methods, it was introduced as corrosion inhibitor withexcellent anti-corrosion action for mild steel (MS) in the simulated seawater. As-obtained results revealthat GA-2 has a significantly enhanced anticorrosion performance, and its inhibitive efficiency reaches98.8% at the 0.5 mmol L1 inhibitor concentration, much higher than that of GA (69.8%) and the intermediatederivative GA-1 (81.2%). The significantly enhanced protection performance could be attributed tothe rich S heteroatoms, aromatic rings, and Schiff base groups in the GA-2, which strongly promotes thechemisorption as well as physisorption between GAD and MS. X-ray photoelectron spectroscopy (XPS)results, show that the appearance of N-Fe and S-Fe bonds confirms the strongly anchoring interactionbetween GA and MS. Furthermore, according to the quantum chemical calculations, the results fromthe lowe energy gap and radical distribution function (RDF) analyses further confirm the strongchemisorption of GA-2 at the surface of steel, which illustrates the excellent inhibition performance atthe molecule and atom level.

Assessment of the relative tectonic activity of the Longxian–Baoji Fault Zone in the northeastern Tibetan Plateau based on geomorphic indices

Qi Huang,Xiaohu Zhou,Jiyuan You,Shuaishuai Xu,Lushan Liu,Yang Wang 한국지질과학협의회 2023 Geosciences Journal Vol.27 No.6

Since the Late Cenozoic, tectonic deformation has been intense in the Longxian–Baoji Fault Zone (LBFZ), which is at the intersection of the southwest margin of the Ordos Block, the northeast margin of the Tibet Plateau, and the Qinling Orogenic Belt. To evaluate the relative tectonic activity within the LBFZ and discuss the influence of the northeastward expansion of the Tibetan Plateau on the geomorphological evolution of the LBFZ, we extracted data of the Qianhe, Hengshuihe, and Jinlinghe River Basins from the ASTER GDEM and analyzed the geomorphic indices, including the hypsometric integral (HI), standardized stream length-gradient index (SL/K) and Hack profile, elongation ratio (Re), the drainage basin asymmetry factor (AF) and valley floor width-to-height ratio (VF). Through geomorphic indices and field investigations, we found that the LBFZ has experienced relatively high tectonic activity. Combined with the index of relative active tectonics (IAT), we compared the tectonic activity strengths of the four major faults in the study area. Among them, the tectonic activities of the Longxian-Qishan Fault (LQF) and the Taoyuan-Guichuansi Fault (TGF) are the highest. The morphology and AF values of the drainage basin of the southwest side of the study area indicate the influence of northwest-southeast compressive stress. The northeastward expansion of the Tibetan Plateau affected the LBFZ region, and the stress brought about by it controlled the tectonic deformation in the region and sculpted the modern landscape. This study is of great significance for understanding the impact of the northeastward expansion of the Tibetan Plateau on the geomorphological evolution of the LBFZ.

Trichloroethylene injures rat liver and elevates the level of peroxisomal bifunctional enzyme (Ehhadh)

Nuanyuan Luo,Qunqun Chang,Xiaohu Ren,Peiwu Huang,Wei Liu,Li Zhou,Yungang Liu,Jianjun Liu 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Trichloroethylene (TCE) is a common industrial solvent and an occupational toxicant. TCE exposure can cause severe hepatotoxicity, but its mode of action is poorly understood. Many studies have shown TCE-induced liver damage in mice, while few have examined the effects of TCE in rats. Objective To explore the effects of TCE in Sprague–Dawley (SD) rats and the potential mechanisms in TCE-induced hepatocytotoxicity. Results The liver index and activities of liver damage marker enzymes (ALT, AST and ALP) in rat serum were elevated along with the increase in TCE dose, while the levels of total proteins and albumin in serum were reduced. The results suggest that TCE is hepatotoxic in rats. 2D-DIGE electrophoresis showed that the levels of 66 liver proteins in TCE-treated rats were abnormally altered (39 up-regulated and 27 down-regulated). In these proteins, six enzymes were related to liver damage and carcinogenesis as indicated by bioinformatics analysis, and Western blot analysis confirmed the alterations of three of them, i.e., aldehyde dehydrogenase 2 (Aldh2), glutathione S-transferase Mu 1 (Gstm1) and peroxisomal bifunctional enzyme (PBE, also named as Ehhadh). PBE was the only protein elevated in a dose dependent manner. Whether PBE can be a biomarker of TCE hepatotoxicity needs to be further studied. Conclusion These findings indicate that TCE induces liver injury in rats.

A Lightweight Pedestrian Intrusion Detection and Warning Method for Intelligent Traffic Security

Xinyun Yan,Zhengran He,Youxiang Huang,Xiaohu Xu,Jie Wang,Xiaofeng Zhou,Chishe Wang,Zhiyi Lu 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.12

As a research hotspot, pedestrian detection has a wide range of applications in the field of computer vision in recent years. However, current pedestrian detection methods have problems such as insufficient detection accuracy and large models that are not suitable for large-scale deployment. In view of these problems mentioned above, a lightweight pedestrian detection and early warning method using a new model called you only look once (Yolov5) is proposed in this paper, which utilizing advantages of Yolov5s model to achieve accurate and fast pedestrian recognition. In addition, this paper also optimizes the loss function of the batch normalization (BN) layer. After sparsification, pruning and fine-tuning, got a lot of optimization, the size of the model on the edge of the computing power is lower equipment can be deployed. Finally, from the experimental data presented in this paper, under the training of the road pedestrian dataset that we collected and processed independently, the Yolov5s model has certain advantages in terms of precision and other indicators compared with traditional single shot multiBox detector (SSD) model and fast region-convolutional neural network (Fast R-CNN) model. After pruning and lightweight, the size of training model is greatly reduced without a significant reduction in accuracy, and the final precision reaches 87%, while the model size is reduced to 7,723 KB.

Restoration of NAD + homeostasis protects C2C12 myoblasts and mouse levator ani muscle from mechanical stress-induced damage

Guotao Huang,Yong He,Li Hong,Min Zhou,Xiaohu Zuo,Zhihan Zhao 한국통합생물학회 2022 Animal cells and systems Vol.26 No.4

Excessive mechanical traction damages the levator ani muscle (LAM), increasing the incidence of pelvic floor dysfunction (PFD). In this study, we explored the effects of oxidized nicotinamide adenine dinucleotide (NAD+) on the damage to both muscle cells and LAM tissue induced by mechanical stress (MS) at the cellular and animal levels. The cell damage model was established using a four-point bending system. The LAM damage model was established using vaginal distention and traction. Exogenous addition of PJ34, an inhibitor of poly (ADP-ribose) polymerase-1 (PARP-1), and the nicotinamide mononucleotide (NMN) precursor of NAD+ increased NAD+ levels. ATP content and mitochondrial membrane potential were measured to assess mitochondrial function. NAD+ levels, cell viability, and PARP-1 activity were detected using commercial kits. DNA damage in cells was detected with immunofluorescence staining, and LAM damage was detected with tissue TUNEL staining. PARP-1 activity and DNA damage of LAM were detected by immunohistochemistry. A small amount of DNA damage and PARP-1 activation did not affect NAD+ levels, while excessive DNA damage and PARP-1 activation led to an imbalance of NAD+ homeostasis. Furthermore, increasing NAD+ levels in vivo and in vitro could rescue mitochondrial dysfunction and damage to both muscle cells and LAM tissue induced by MS. In conclusion, MS can induce damage to both C2C12 cells and LAM tissue. Restoring NAD+ homeostasis can rescue this damage by improving mitochondrial function.