AFB₁ 대사에서 phloretion의 이중 활성 효과

임대원,이광,고상상,진효연,은상용,최병민,김복량 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.1

Aflatoxm B₁(AFB₁) is a potent hepatocarcinogen in experimental animals and a hazard to human health in several parts of the world. AFB₁ is activated to its ultimate carcinogenic intermediate, AFB₁-8,9-epoxide, by cytochrome P450(CYP) 1A2 and CYP3A4 in human liver and the intermediate is decomposed by several glutathione S-transferase(GST) including GSTA2, GSTM1 and GSTP1. In this study, we investigated the effects of phloretin on the enzyme systems which are involved in the activation and detoxification of AFB₁. The metabolic intermediate of AFB₁ was measured with HPLC. We found that phloretin could strongly inhibit the activities of CYP 3A4 and CYP1A2 in a dose dependent manner. Phloretin induced the antioxidant-response element(ARE)-mediated gene expression, including GSTs. The expressions of GSTA2, T1, M1, and GSTP1 were induced by 10μM phloretin. The decomposition of AFB₁-8,9-epoxide was measured with GSH conjugating activity of the epoxide. The rate was increased to 1.5 fold when HepG2 cells were treated by 10μM phloretin for 12h. In the mean while, the total GST activitives toward CDNB in HepG2 cells were not changed by the treatment with phloretin. The results demonstrate that phloretin has strong chemopreventive effects against AFB₁ toxicity through the inhibition of AFB₁ activation and induction of GSTs.

Facile Self-Assembly of Fe3O4 Nanoparticles@WS2 Nanosheets: A Promising Candidate for Supercapacitor Electrode

Yu Dai,Xiao Wu,Dawei Sha,Ming Chen,Han Zou,Jie Ren,Jingjing Wang,Xuehua Yan 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6

Graphene-like dichalcogenides with huge surface area and nanostructuredtransition metal oxides with extraordinarily high theoretical capacities could becomposited as promising electrode candidates for supercapacitors. In this work,monolayer and few-layers WS2 nanosheets were exfoliated by combination ofball-milling and sonication. A facile strategy for the hierarchical self-assembly ofFe3O4 nanoparticles (Fe3O4NPs) on WS2 nanosheets was developed to synthesizeFe3O4NPs@WS2 nanocomposites via hydrothermal method. Fe3O4NPs areuniformly dispersed on the WS2 nanosheets without aggregation. The particlesize of Fe3O4NPs is about 3 nm. The nanocomposite shows strong enhancementsof electrochemical behaviors. This self-assembly synthesis strategy may havegreat prospects for other 0D/2D nanocomposites in supercapacitors and otherenergy devices.

Integration Sites and Genotype Distributions of Human Papillomavirus in Cervical Intraepithelial Neoplasia

Wang, Li,Dai, Shu-Zhen,Chu, Hui-Jun,Cui, Hong-Fei,Xu, Xiao-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Objectives: To analyse HPV integration prevalence and genotype distributions in cervical intraepithelial neoplasia (CIN) in east part of China, furthermore to assess preferential sites for common HPV integrations and provide baseline information for cervical abnormality screening and prevention. Methods: Integration of HPV in 113 paraffin-embedded cervical intraepithelial neoplasia samples was assessed using Gencap technology in Key Laboratory of Biotechnologies in BGI-Shenzhen. Results: 64 samples were HPV-integrated and as the cervical lesions increased, the integration rate became higher significantly (P=0.002). Fifteen different HPV genotypes were detected, 14 high-risk (16, 18, 31, 33, 51, 52, 56, 58, 66, 68) and 1 low-risk (11). The most common genotypes were HPV-16, 58, 33, 52, 66, and 56. Thirteen patients had co-integration involving mainly HPV-16 and 58. The frequency of HPV gene disruption was higher in L1 and E1 genes than in other regions of the viral genomes. Conclusion: Some 56.6% of CIN lesions in Qingdao had HPV integrations, and 67.2% of HPV-integrated patients were HPV-16 and 58, more prone to be integrated in younger patients below 45 years old. There exist preferential sites for HPV-16 and HPV-58 integration, and they are more likely to be disrupted in the L1 and E1 loci.

Characteristics of High-cell Density Cultivation of Recombinant Escherichia coli Producing RHLC Using the Fermentor Pressure Shifting Strategy

Lei Chi,Dai-Di Fan,Xiao-Xuan Ma,Yan-E Luo,Chen-Hui Zhu 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3

To increase the biomass and production of recombinant human-like collagen (RHLC), the effect of controlled fermentor pressure during fed-batch cultivation was investigated using recombinant Escherichia coli producing RHLC. This study focused primarily on the effects of the fermentor pressure on the oxygen transfer capacity. A twostep exponential feeding strategy was used to control the specific growth rate at 0.2 and 0.1/h in the fed-batch and induction phase, respectively. A kinetic model of cell growth was developed, and the specific growth rate, specific glucose uptake rate, concentration of extracellular DNA, and percentage of plasmid loss were calculated and detected. The results demonstrated that increasing the fermentor pressure was an effective way of avoiding the oxygen transfer capacity limitation, and an increase in the dissolved CO2 content did not affect the growth of the recombinant E. coli BL21strain. At the end of the fermentation process, the cell density (represented by the dry cell weight, DCW) reached 77.3 g/L, and the RHLC concentration reached 14.1 g/L. In addition,the oxygen transfer capacity (KLaC^*) decreased drastically at approximately 5 h after induction. This is probably because of the increased concentration of extracellular DNA due to cell lysis, indicating that the cells needed to be harvested.

Kinetic Changes and Antihypertensive Effect of Aqueous Extract of Danggui (Angelica sinensis Radix) after Stir-fry Processing

Gui-Fen Zhou,Xiao-Yan Dai,Chen-Huan Yu,Jie Fang 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.3

Danggui (Angelica sinensis Radix) in East Asia for its unique pleasant flavor is usually cooked before utilizing to enhance its tonic effect. To evaluate the quality and bioactivity changes, heat treatments were carried out at 250oC for 0-45 min. 5-Hydroxymaltol, 5-hydroxymethylfurfural,Z-ligustilide, and ferulic acid in aqueous extract of processed danggui (APD) were determined by HPLC while antihypertensive effects were studied after oral administration of APD (50 and 100 mg/kg) for 3 days in spontaneously hypertensive rats (SHR). Compared with aqueous extract of danggui (AD), contents of ferulic acid and Z-ligustilide in APD were significantly decreased but 5-hydroxymaltol and 5-hydroxymethylfurfural were increased. APD could lower blood pressures, plasma renin activities, and angiotensin II levels of SHR, but AD failed to do those. APD exhibited hypotensive effects by regulating renin-angiotensin system and may warrant further evaluation as a potential antihypertensive agent.

Analytical and Numerical Studies on Steel Columns with Novel Connections in Modular Construction

En-Feng Deng,Jia-Bao Yan,Yang Ding,Liang Zong,Zhong-Xian Li,Xiao-Meng Dai 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.4

modular construction is a new type of promising structures. This type of structure is an excellent alternative to conventional on-site buildings with advantages of shortened construction time, improved construction quality and reduced environmental pollution. A new concept of modular construction with novel connections has been proposed. This paper mainly focuses on the behavior and design of the innovative steel column working together with tenons at both ends. The fourthdifferential equation was firstly adopted to develop a theoretical model to determine the buckling length of the column. Then, a 3-D nonlinear finite element (FE) model was developed to simulate the ultimate strength behavior of the novel column under pure compression. Parametric studies reveal that the tenons play a significant role in affecting the ultimate load and the corresponding end shortening, among which the length of the tenons is the most important factor. The design approach and recommendations are also discussed through assessing the applicability of the current design code.

Inhibitory effects of piceatannol on human cytomegalovirus (hCMV) in vitro

Wang San-Ying,Zhang Jing,Xu Xiao-Gang,Su Hui-Li,Xing Wen-Min,Zhang Zhong-Shan,Jin Wei-Hua,Dai Ji-Huan,Wang Ya-Zhen,He Xin-Yue,Sun Chuan,Yan Jing,Mao Gen-Xiang 한국미생물학회 2020 The journal of microbiology Vol.58 No.8

Human cytomegalovirus (hCMV) is a ubiquitous herpesvirus, which results in the establishment of a latent infection that persists throughout the life of the host and can be reactivated when the immunity is low. Currently, there is no vaccine for hCMV infection, and the licensed antiviral drugs mainly target the viral enzymes and have obvious adverse reactions. Thus, it is important to search for compounds with antihCMV properties. The present study aimed to investigate the suppressive effects of piceatannol on hCMV Towne strain infection and the putative underlying mechanisms using human diploid fibroblast WI-38 cells. Piceatannol supplementation prevented the lytic changes induced by hCMV infection in WI-38 cells. Furthermore, piceatannol suppressed the expression of hCMV immediate-early (IE) and early (E) proteins as well as the replication of hCMV DNA in a dose-dependent manner. Moreover, hCMV-induced cellular senescence was suppressed by piceatannol, as shown by a decline in the senescence-associated β-galactosidase (SA-β-Gal) activity and decreased production of intracellular reactive oxygen species (ROS). p16INK4a, a major senescence-associated molecule, was dramatically elevated by current hCMV infection that was attenuated by pre-incubation with piceatannol in a dose-dependent manner. These results demonstrated that piceatannol suppressed the hCMV infection via inhibition of the activation of p16INK4a and cellular senescence induced by hCMV. Together, these findings indicate piceatannol as a novel and potent anti-hCMV agent with the potential to be developed as an effective treatment for chronic hCMV infection.

Establishment of a novel myocarditis mouse model based on cyclosporine A

Zhao Tian Hao,Jiang Yi Xuan,Chen Kai Qin,Qiu Dan,Xu Yan Zhe,Ye Chun,Ren Ting,Zhang Bo,Dai Bin,Hu Jue,Lu Jun,Zhou Fang Liang,Xiao Rong,Lu Fang Guo,Wei Ke 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Myocarditis is a myocardial injury that can easily cause adolescent death. Traditional research models of animal invasion with viral components, lipopolysaccharide (LPS) or porcine myocardial myosin, among others, have the shortcomings of potential biological safety hazards and high animal mortality. Objective: To explore the construction of a novel myocarditis model with cyclosporine A and the potential genes and pathways associated with it. Methods: BALB/c mice were used in this study, and cyclosporin A and LPS were injected into the peritoneal cavity of mice. The successful establishment of the model was assessed by detecting serum myocardial injury markers and inflammatory factors levels, HE, IHC staining, and RT-qPCR methods. Key genes were obtained using the GSE35182 dataset from the GEO database and validated with the RT-qPCR method. Results: We found that a large number of inflammatory cells infiltrated the myocardium of mice in each group of Cyclosporin A constructed model, while the expression of inflammatory factor indicators was increased, and this model has the characteristics of high degree of local inflammation in myocardial tissue, low mortality, and safe and non-toxic treatment. Using GSE35182 data, we selected 18 Hub genes and validated Hub genes in myocardial tissue with RT-qPCR and found that multiple signaling pathways such as Toll-likereceptor signaling pathway(TLRs), Rap1 signal pathway(Rap1), and Chemokine signaling pathway may be involved in the development of myocarditis. Conclusion: Cyclosporin A can construct a new myocarditis model, and TLRs, Chemokines and Rap1 signaling pathways may be the core pathways of myocarditis.

Efficacy and Safety of Sorafenib for Advanced Non-Small Cell Lung Cancer: a Meta-analysis of Randomized Controlled Trials

Wang, Wei-Lan,Tang, Zhi-Hui,Xie, Ting-Ting,Xiao, Bing-Kun,Zhang, Xin-Yu,Guo, Dai-Hong,Wang, Dong-Xiao,Pei, Fei,Si, Hai-Yan,Zhu, Man Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Many clinical trials have been conducted to evaluate sorafenib for the treatment of advanced NSCLC, but the results for efficacy have been inconsistent. The aim of this study was to evaluate the efficacy and safety of sorafenib in patients with advanced NSCLC in more detail by meta-analysis. Methods: This meta-analysis of randomized controlled trials (RCTs) was performed after searching PubMed, EMBASE, ASCO Abstracts, ESMO Abstracts, and the proceedings of major conferences for relevant clinical trials. Two reviewers independently assessed the quality of the trials. Outcomes analysis were disease control rate (DCR), progression- free survival (PFS), overall survival (OS) with 95% confidence intervals (CI) and major toxicity. Subgroup analysis was conducted according to sorafenib monotherapy, in combination with chemotherapy or EGFR-TKI to investigate the preferred therapy strategy. Results: Results reported from 6 RCTs involving 2, 748 patients were included in the analysis. Compared to sorafenib-free group, SBT was not associated with higher DCR (RR 1.31 (0.96- 1.79), p=0.09), PFS (HR 0.82 (0.66-1.02), p=0.07) and OS (HR 1.01 (0.92-1.12), p=0.77). In terms of subgroup results, sorafenib monotherapy was associated with significant superior DCR and longer PFS, but failed to show advantage with regard to OS. Grade 3 or greater sorafenib-related adverse events included fatigue, hypertension, diarrhea, oral mucositis, rash and HFSR. Conclusions: SBT was revealed to yield no improvement in DCR, PFS and OS. However, sorafenib as monotherapy showed some activity in NSCLC. Further evaluation may be considered in subsets of patients who may benefit from this treatment. Sorafenib combined inhibition therapy should be limited unless the choice of platinum-doublet regimen, administration sequence or identification of predictive biomarkers are considered to receive better anti-tumor activity and prevention of resistance mechanisms.