Pyramiding of 2 QTLs, gw8 and gw9, underlying grain weight using nearly isogenic lines (NILs) in rice

Feng-Xue Jin,Xiao-Bo Xie,Shi-Dong Ji,Hong-Guang Ju,Jung-Pil Suh,Hung-Goo Hwang,Sang-Nag Ahn 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Grain weight (GW) or grain size is the most important target not only as a major component of grain yield, but also associated with the cooking quality in rice breeding program. In a previous study, two loci for grain weight, gw8 and gw9, have been fine map to about 306.4kb and 37.4kb, respectively, in backcross populations derived from a cross between the Korean japonica cultivar Hwaseongbyeo and Oryza rufipigon (IRGC 105491). Both O.rufipogon alleles increased GW in the Hwaseongbyeo background despite the fact that O. rufipogon was the small-seeded parent. To test the effect of pyramiding the QTLs for grain weight, an F2 population consisted of 226 individuals was developed derived from the cross between the two QTLs (gw8 and gw9), and used MAS to select nearly isgenic lines for two QTLs and a pyramiding line. Molecular genotyping of F2 population revealed the existence of interaction between gw8 and gw9. Moreover, the interaction was also confirmed by phenotypic analysis of four QTL-NILs. The results suggest that epistasis interaction is important genetic basis in determining yield traits in rice.

Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China

Xiao W. Ji,Ya L. Liao,Ye F. Zhu,Hai G. Wang,Ling Gu,Jiang Gu,Chen Dong,Hong L. Ding,Xu H. Mao,Feng C. Zhu,Quan M. Zou 한국미생물학회 2010 The journal of microbiology Vol.48 No.6

Escherichia coli O157:H7, an important food-borne pathogen, has become a major public health concern worldwide. The aim of this study was to investigate the molecular epidemiologic feature of E. coli O157:H7strains in China. 105 E. coli O157:H7 isolates were collected from various hosts and places over 9 years. A multilocus sequence typing scheme (MLST) was applied for bacteria genotyping and polymerase chain reaction (PCR) was used for virulence factor identification. Seven new MLST sequence types (STs), namely ST836, ST837, ST838, ST839, ST840, ST841, and ST842 were identified, which grouped into two lineages. Phylogenetic analysis suggested that the most two frequent STs in China, ST837 and ST836, may be the derivatives of E. coli O157:H7 Sakai or E. coli O157:H7 EDL933. Geographical diversity and host variety of E. coli O157:H7 were observed in China. In addition, the different distribution of tccp was detected. The data presented herein provide new insights into the molecular epidemiologic feature of E. coli O157:H7, and aid in the investigation of the transmission regularity and evolutionary mechanism of E. coli O157:H7.

Seismic behavior of cross-shaped concrete-filled steel tubular columns

Ji-Cheng Zhang,Xiao-Yu Liu,Lei Zeng,Guo-Feng Du,Jia-Hao Xiao 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.3

This paper experimentally investigated the behavior of a cross-shaped concrete-filled steel tubular (C-CFST) column subjected to a constant axial load and a low-cycle repeated loading. Nine C-CFST columns with different length-width ratio, width-thickness ratio and axial compression ratio were designed, and the failure mode, hysteresis curve, skeleton curve, ductility, stiffness degradation and energy dissipation capacity of each specimen were studied and analyzed. The results indicated that the cross-shaped steel tube had a strong restraining effect on the core concrete, and C-CFST columns of different sectional dimensions all exhibited favorable seismic behavior, which is suitable for middle-high residential buildings. An increase of length-width ratio enhanced the initial stiffness with a decrease of ductility, and more rapid stiffness degradation during loading. Specimens with smaller width-thickness ratios had higher ductility, stiffness, and energy dissipation capacity. A larger axial compression ratio could reduce the bearing capacity, and cause the stiffness to degrade faster. Moreover, a hysteretic model of C-CFST columns was also proposed based on an analysis of the test results.

AUTOMATIC DETECTION AND EXTRACTION ALGORITHM OF INTER-GRANULAR BRIGHT POINTS

Feng, Song,Ji, Kai-Fan,Deng, Hui,Wang, Feng,Fu, Xiao-Dong The Korean Astronomical Society 2012 Journal of The Korean Astronomical Society Vol.45 No.6

Inter-granular Bright Points (igBPs) are small-scale objects in the Solar photosphere which can be seen within dark inter-granular lanes. We present a new algorithm to automatically detect and extract igBPs. Laplacian and Morphological Dilation (LMD) technique is employed by the algorithm. It involves three basic processing steps: (1) obtaining candidate "seed" regions by Laplacian; (2) determining the boundary and size of igBPs by morphological dilation; (3) discarding brighter granules by a probability criterion. For validating our algorithm, we used the observed samples of the Dutch Open Telescope (DOT), collected on April 12, 2007. They contain 180 high-resolution images, and each has a $85{\times}68\;arcsec^2$ field of view (FOV). Two important results are obtained: first, the identified rate of igBPs reaches 95% and is higher than previous results; second, the diameter distribution is $220{\pm}25km$, which is fully consistent with previously published data. We conclude that the presented algorithm can detect and extract igBPs automatically and effectively.

K-Charmonium Dissociation Cross Sections and Charmonium Dissociation Rates in Hadronic Matter

Feng-Rong Liu,Shi-Tao Ji,Xiao-Ming Xu 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.69 No.4

K-charmonium dissociation reactions in hadronic matter are studied in the Born approximation, in the quark-interchange mechanism, and with a temperature-dependent quark potential. We obtain the temperature dependence of the unpolarized cross sections for the reactions KJ/ ! ¯D D+ s , ¯D D+ s , ¯DD+ s , and ¯DD+ s ; K 0 ! ¯DD+ s , ¯DD+ s , ¯DD+ s , and ¯DD+ s ; Kc ! ¯DD+ s , ¯DD+ s , ¯D D+ s , and ¯D D+ s . We use the cross sections for charmonium dissociation in collisions with pions, mesons, kaons, vector kaons, and mesons to calculate the dissociation rates of charmonium with five types of mesons. Because of the temperature dependence of the meson masses, dissociation cross sections, and meson distribution functions, the charmonium dissociation rates generally increase with increasing temperature and decrease with increasing charmonium momentum from 2.2 GeV/c. We find that the first derivative of the dissociation rate with respect to the charmonium momentum is zero when the charmonium is at rest. While the + 0 and the + c dissociation reactions can be neglected, the J/ , 0, and c dissociations are caused by collisions with pions, mesons, kaons, vector kaons, and mesons.

Drying Characteristics and Models of Red Ginseng Slice Using Far-Infrared Rays

Ning Xiao Feng,Xu Ji Tong,장문기 한국농업기계학회 2021 바이오시스템공학 Vol.46 No.4

Purpose The aim of this study was to improve the drying quality and find the suitable drying model for the far-infrared drying of red ginseng slice. Methods The far-infrared drying tests on ginseng slice were conducted at two slice thickness of 2 and 4 mm and three drying temperatures of 50, 60, and 70 °C. The results were compared with the hot air drying method. The drying characteristics were analyzed based on factors such as drying rate, color changes, and elastic modulus. The Page, modified Wang and Singh, and logarithmic models were used to select the best model for far-infrared drying of red ginseng slices. Results The results revealed that increasing drying temperature and decreasing ginseng thickness caused a decrease in drying time. The color differences (ΔE) at 70 °C values were higher than 50 and 60 °C values. The highest elastic modulus (32.42 MPa) was observed at drying temperatures of 60 °C ginseng slice of 4mm. The drying quality of far-infrared dryingwas better than that of hot air drying under the same drying condition. The Page model showed the highest R2 values and lowest RMSE values than the other models. Conclusion The quality of red ginseng slice can be improved by far-infrared drying. The Page model was the best model to fit adequately most of the drying conditions for the far-infrared drying of red ginseng slice.

Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p

Lu, Feng-Bin,Chen, Da-Zhi,Chen, Lu,Hu, En-De,Wu, Jin-Lu,Li, Hui,Gong, Yue-Wen,Lin, Zhuo,Wang, Xiao-Dong,Li, Ji,Jin, Xiao-Ya,Xu, Lan-Man,Chen, Yong-Ping Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.12

MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis.

Detection of epistatic interaction of two QTLs, <i>gw8.1</i> and <i>gw9.1</i>, underlying grain weight using nearly isogenic lines in rice

Jin, Feng-Xue,Ji, Shi-Dong,Xie, Xiao-Bo,Kang, Ju-Won,Ju, Hong-Guang,Ahn, Sang-Nag Japanese Society of Breeding 2011 Breeding science Vol.61 No.1

<P>Grain weight (GW) is one of the most important targets for grain yield in rice breeding. In previous studies, two quantitative trait loci (QTLs) for GW, <I>gw8.1</I> and <I>gw9.1</I>, have been identified using progeny derived from a cross between the <I>japonica</I> cultivar Hwaseong and <I>Oryza rufipogon </I>(IRGC 105491). To test whether these quantitative trait loci (QTLs) have an epistatic interaction, we developed an F<SUB>2</SUB> population by crossing two nearly isogenic lines (NILs) harboring <I>gw8.1</I> and <I>gw9.1</I>. Simple sequence repeat (SSR) markers tightly linked to the QTLs were used to select F<SUB>3</SUB> QTL-NILs from the F<SUB>2</SUB> population. A two-way ANOVA revealed an epistatic interaction between the two QTLs in the F<SUB>2</SUB> population (<I>P</I> = 0.0084). This interaction was confirmed by an analysis of F<SUB>3</SUB> QTL-NILs indicating that both QTLs are involved in the same genetic mechanism controlling GW. The <I>gw8.1</I> QTL was further mapped between two SSR markers, RM23204 and RM23211, which are 110.1 kb apart. To our knowledge, this is the first report using QTL-NILs to reveal an epistatic interaction between QTLs for GW.</P>

Detection of Cucumber Powdery Mildew Based on Spectral and Image Information

Xu Ji Tong,Zhang Zhe,Guo Yu Hang,Liu Yufeng,Ning Xiao Feng 한국농업기계학회 2023 바이오시스템공학 Vol.48 No.2

Purpose The aim of this study was to fi nd the optimal detection method for cucumber powdery mildew and improve the identifi cation effi ciency. Methods Image segmentation technology was used to extract spot images and grade classifi cation of powdery mildew. The relationship between powdery mildew spot and spectral refl ectance and intensity was studied. The powdery mildew detection model was established by using qualitative analysis and quantitative prediction methods combined with greenness ( a* ) indices of cucumber leaves. Results There were strong positive correlations between greenness and spectrum in some characteristic bands. Through the extraction of disease spot images and disease classifi cation, it was found that the higher the disease grade of leaves was, the higher the spectral refl ectivity and fl uorescence intensity. In the quantitative prediction model, the R 2 of the NIR spectrum was improved (0.8742) after MSC and SPA, and the eff ect of the fl uorescence spectrum model was not ideal. In the qualitative discriminant model, KNN and ensemble subspace discriminant were obtained for two kinds of spectra, and the identifi cation accuracy of the qualitative model was 97.5% after verifi cation. Conclusion An NIR spectral model can be used for the quantitative prediction of cucumber powdery mildew. The qualitative discriminant model had high accuracy for cucumber powdery mildew.

Value of Postoperative Radiation Therapy for Regional Control after Dissection in Head and Neck Squamous Cell Carcinoma Cases

Li, Xiao-Ming,Di, Bin,Shang, Yao-Dong,Tao, Zhen-Feng,Cheng, Ji-Min,He, Zhan-Guo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Objective: We aimed to define clinicopathologic risk factors associated with regional recurrence (RR) and thus the effectiveness of postoperative radiotherapy (PORT) for neck control for head and neck squamous cell carcinomas (HNSCCs) with differing cervical lymph node status. Methods: A retrospective study was performed in 196 HNSCC patients with pathologically positive neck node (N+) to evaluate the high-risk factors for RR and to define the role of PORT in control after neck dissection and postoperative radiotherapy (PORT). Results: Overall, the RR rate after neck dissection and PORT was 29%. Extracapsular spread (ECS) was confirmed to be the only independent risk factor for RR. There were no significant risk factors associated with RR in the ECS- group. The 5-year disease-specific survival rate was 45%, which descended to 10% with the emergence of RR. Conclusions: ECS remains a determined risk factor for RR after neck dissection and PORT in patients with N+. PORT alone is not adequate for preventing RR in the neck with ECS after neck dissection. More intensive postoperative adjuvant therapies, especially combined chemotherapy and radiotherapy, are needed to prevent regional failure in HNSCC patients with ECS.